Literature
PubmedId Abstract Title Authors PMCId
0 not available not available not available not available
537276 Vasodepressor mechanism of lycorenine (an alkaloid of Lycoris radiata Herb.) was investigated in anesthetized rats. Lycorenine (1--10 mg/kg i.v.) produced dose-related decreases in blood pressure and heart rate and tachyphylaxis developed with repeated injections. In the blood-perfused rat hindquarters, lycorenine (62.5--500 micrograms i.a.) produced dose-related decreases both in mean blood pressure and in perfusion pressure, and the lycorenine-induced decrease in perfusion pressure was abolished by phenoxybenzamine or hexamethonium. Lycorenine (more than 1 mg/kg i.v.) blocked the pressor response to sympathetic nerve stimulation, but failed to block the tachycardia induced by sympathetic nerve stimulation. Lycorenine (7.5 or 15 mg/kg i.v.) reduced the spontaneous splanchnic nerve activity. Lycorenine when given intracerebroventricularly produced decreases in blood pressure and heart rate only in large doses (over 500 micrograms). The maximal bradycardia induced by lycorenine was abolished by bilateral vagotomy. It is suggested that lycorenine may produce a decrease in blood pressure as the result of alpha-adrenergic blockade in conjunction with the reduction of the spontaneous sympathetic nerve activity, and produce bradycardia by modifying vagal activity. Pharmacological studies of lycorenine, an alkaloid of Lycoris radiata Herb.: Vasodepressor mechanism in rats. Miyasaka K, Hiramatsu Y, Takezaki T. No
1195115 Column chromatography on silica gel of a petroleum ether extract of the flowering heads of Achillea millefolium L. allowed three flavones to be separated and identified. Spectral studies (PMR, mass spectrometry, and UV) and a comparison with data for compounds reported in the literature established the flavones as 5-hydroxy-3,6,7,4'-tetramethoxyflavone, artemetin, and casticin. These compounds have not been reported previously as constituents of A. millefolium. Isolation and identification of three new flavones from Achillea millefolium L. Falk AJ, Smolenski SJ, Bauer L, Bell CL No
1368420 Besides isolation and characterization of isorhamnetin-3-O-rutinoside, isorhamnetin-3-O-glucopyranoside and taraxasteryl acetate, a new flavonol trisaccharide was isolated from the aerial parts of Calotropis gigantea, and its structure was established as isorhamnetin-3-O-[2-O-beta-D-galactopyranosyl-6-O-alpha-L-rhamnopy ranosyl]- beta-D-glucopyranoside by a combination of fast atom bombardment mass spectroscopy, 1H and 13C NMR spectra and some chemical degradations. Flavonol glycosides from Calotropis gigantea. Sen S, Sahu NP, Mahato SB. No
1468110 In our search for therapeutic agents from natural sources with potential for the treatment of opportunistic infections in patients afflicted with acquired immunodeficiency syndrome (AIDS), we investigated antibacterial and antifungal activities of water extracts of Cassia alata (C. alata). The extracts are traditionally used in Ivory Coast, West Africa to treat bacterial infections caused by Escherichia coli (E. coli), and fungal infections caused by Candida albicans (C. albicans) and dermatophytes. Our working hypothesis was that the extract contains active ingredient(s) which can be isolated, identified and developed into useful antimicrobial/antifungal agents for the treatment of opportunistic infections in patients with AIDS. We used the broth dilution and agar dilution methods. Specifically, we focused on E. coli and C. albicans and the effectiveness of the extracts was evaluated relative to those of standard antibacterial agent chloramphenicol and antifungal agent amphotericin B. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for the water extract of C. alata against E. coli were 1.6 mg/ml and 60 mg/ml respectively; corresponding data for chloramphenicol were 2 ug/ml. Similarly, the MIC and minimum fungicidal concentration (MFC) for the extract against C. albicans were 0.39 mg/ml and 60 mg/ml in contrast to 0.58 ug/ml and 0.98 ug/ml for amphotericin B. From the dose-response curve plots, the extract had an IC50 of 31 mg/ml for E. coli and 28 mg/ml for C. albicans. The data suggest that C. alata extracts contain agent(s) which have therapeutic potential and might be useful if isolated and developed for the treatment of opportunistic infections of AIDS patients. Cassia alata and the preclinical search for therapeutic agents for the treatment of opportunistic infections in AIDS patients. Crockett CO, Guede-Guina F, Pugh D, Vangah-Manda M, Robinson TJ, Olubadewo JO, Ochillo RF. No
1591991 CONTEXT:Eucalyptus globulus Labill (Myrtaceae) is the principal source of eucalyptus oil in the world and has been used as an antiseptic and for relieving symptoms of cough, cold, sore throat, and other infections. The oil, well known as 'eucalyptus oil' commercially, has been produced from the leaves. Biological properties of the essential oil of fruits from E. globulus have not been investigated much.OBJECTIVE:The present study was performed to examine the antimicrobial activity of the fruit oil of E. globulus (EGF) and the leaf oils of E. globulus (EGL), E. radiata Sieber ex DC (ERL) and E. citriodora Hook (ECL) against multidrug-resistant (MDR) bacteria. Furthermore, this study was attempted to characterize the oils as well as to establish a relationship between the chemical composition and the corresponding antimicrobial properties.MATERIALS AND METHODS:The chemical composition of the oils was analyzed by GLC-MS. The oils and isolated major components of the oils were tested against MDR bacteria using the broth microdilution method.RESULTS:EGF exerted the most pronounced activity against methicillin-resistant Staphylococcus aureus (MIC ~ 250 µg/ml). EGF mainly consisted of aromadendrene (31.17%), whereas ECL had citronellal (90.07%) and citronellol (4.32%) as the major compounds. 1,8-cineole was most abundant in EGL (86.51%) and ERL (82.66%.DISCUSSION AND CONCLUSION:The activity of the oils can be ranked as EGF > ECL > ERL ~ EGL. However, all the oils and the components were hardly active against MDR Gram-negative bacteria. Aromadendrene was found to be the most active, followed by citronellol, citronellal and 1,8-cineole. Antibacterial activity of essential oils from Eucalyptus and of selected components against multidrug-resistant bacterial pathogens. Mulyaningsih S, Sporer F, Reichling J, Wink M. No
1654576 A reinvestigation of the aerial parts of Calendula arvensis afforded, in addition to the oleanolic acid glycosides 1-4 (4), the new glycoside 5 whose structure was elucidated by spectral and chemical studies and determined as 3-O-(beta-D-galactopyranosyl-(1----3) [beta-D-glucopyranosyl-(1----4)]-beta-D-glucopyranosyl) oleanolic acid (28----1)-beta-D-glucopyranosyl ester. Furthermore, some antiviral tests were performed on glycosides 1-5 and on 5a, the hydrolysis product of 5, towards vesicular stomatitis virus (VSV) and rhinovirus (HRV) infection in cell cultures. An inhibitory effect against VSV multiplication was observed for all the compounds tested while HRV replication was significantly affected only by compound 3. Structure and in vitro antiviral activity of triterpenoid saponins from Calendula arvensis De Tommasi N, Conti C, Stein ML, Pizza C No
1982768 From the antitumor-bioactive sap of Pogonopus speciosus, tubulosine [1] was isolated, by activity-directed fractionation using the brine shrimp lethality test, as the major antitumor constituent. 1H-nmr assignments, obtained from HETCOR and COSY, and X-ray crystallographic results are reported for the first time. Psychotrine [2] was also isolated, and its spectral data are also reported. Tubulosine: an antitumor constituent of Pogonopus speciosus. Ma WW, Anderson JE, McKenzie AT, Byrn SR, McLaughlin JL, Hudson MS. No
2204784 Thirteen saponins were isolated and identified from Calendula officinalis, C. arvensis and Hedera helix. Mutagenic and antimutagenic activities of these products were investigated using a modified liquid incubation technique of the Salmonella/microsomal assay. The Salmonella tester strain TA98 +/- S9 mix was used. Screening of the antimutagenic activity was performed with a known promutagen: benzo-[a]pyrene (BaP) and a mutagenic urine concentrate from a smoker (SU). Antimutagenic activities were also compared with the activity of chlorophyllin. All the saponins were found to be non-toxic and non-mutagenic for doses of 400 micrograms. Chlorophyllin inhibited the mutagenic activities of BaP (1 microgram) and SU (5 microliters) in a dose-dependent manner. The four saponins from C. arvensis and the three saponins from H. helix showed antimutagenic activity against BaP (1 microgram) and SU (5 microliters) with a dose-response relationship. The possible mechanism of the antimutagenic activity of saponins is discussed. Antimutagenic activity of some saponins isolated from Calendula officinalis L., C. arvensis L. and Hedera helix L Elias R, De Méo M, Vidal-Ollivier E, Laget M, Balansard G, Dumenil G No
2236289 Tanzanian medicinal plants were extracted and tested for in vitro antimalarial activity, using the multidrug resistant K1 strain of Plasmodium falciparum. Of 49 plants investigated, extracts of three plants were found to have an IC50 between 5-10 micrograms/ml, extracts of 18 other plants showed an IC50 between 10 and 50 micrograms/ml, all others were less active. The three most active extracts were obtained from the tubers of Cyperus rotundus L. (Cyperaceae), the rootbark of Hoslundia opposita Vahl. (Labiatae), and the rootbark of Lantana camara L. (Verbenaceae). Antimalarial activity of Tanzanian medicinal plants. Weenen H, Nkunya MH, Bray DH, Mwasumbi LB, Kinabo LS, Kilimali VA. No
2332827 The study of the chemical composition of Calendula arvensis var. eu arvensis Maire reveals the presence of saponins. These saponins confer a haemolytic property on the plant. In our work we have, first determined the saponin responsible for the haemolytic property; and then we have studied the impact of the saponins on the acute toxicity and the anti-inflammatory activity induced by carrhageenin oedema. Calendula arvensis L. Impact of saponins on toxicity, hemolytic effect, and anti-inflammatory activity Chemli R, Toumi A, Oueslati S, Zouaghi H, Boukef K, Balansard G No
2932521
The anti-mycobacterial activity of Lantana camara a plant traditionally used to treat symptoms of tuberculosis in South-western Uganda
PMC2932521
3004181 The juice from the leaves of Bryophyllum pinnatum S. Kurtz (Crassulaceae) was tested for antibacterial activity. The extract at 5% v/v was bactericidal to a wide spectrum of Gram-positive and Gram-negative bacteria such as Bacillus subtilis, Staphyllococcus aureus, Streptococcus pyogenes; Streptococcus faecalis; Escherichia coli; Proteus spp; Klebsiella spp; Shigella spp; Salmonella spp; Serratia marcescens; and Pseudomonas aeruginosa including the clinical isolates of these organisms possessing multiple antibiotic resistance. Preliminary report on the in vitro antibacterial activity of Bryophyllum pinnatum leaf juice. Obaseiki-Ebor EE. No
3236009 Polypodoside A, a novel intensely sweet constituent of the rhizomes of Polypodium glycyrrhiza, was established by spectral and chemical methods as 26-O-alpha-L-rhamnopyranosyl-polypodogenin-3-O-alpha-L-rhamnopyran osyl- (1----2)-beta-D-glucopyranoside [1]. At the doses tested, this isolate was not acutely toxic for mice and was nonmutagenic with Salmonella typhimurium strain TM677. This compound was rated by a human taste panel as exhibiting 600 times the sweetness intensity of a 6% w/v aqueous sucrose solution. Polypodoside A, an intensely sweet constituent of the rhizomes of Polypodium glycyrrhiza. Kim J, Pezzuto JM, Soejarto DD, Lang FA, Kinghorn AD. No
3832714 Various extracts of one hundred and eight medicinal plants were screened for their anti-implantation activity in female albino rats. Out of these, 50% ethanolic extract of Codonospis ovata Benth (PL); 50% ethanolic, acetone and benzene extracts of Puararia tuberosa DC (TUB); aqueous and methanolic extracts of Punica granatum Linn. (PX) and ethanolic and acetone extracts of Rubus ellipiticus Smith (PX) inhibited pregnancy in 70-90% of rats. Similarly ethanolic extract of Adhatoda vasica Nees (LF) and Kigelia pinnata DC (PL); ethanolic and acetone extracts of Acrostichum aureum Linn. (PL), Juniperus communis Linn. (SD), Lepidium capitatum H.f. & T. (PL); ethanolic and benzene extracts of Citrulus colocynthus Schrad (LF) and acetone extract of Codonopsis ovata Benth (PL) showed 60-70% anti-implantation activity. Extracts of a few plants VIZ. Dolichos biflorus Linn. (SD), Ferule orientalis Linn. (PL), Nerium odoratum Lamk (RT), Randia dumetorum Lamk (SD) and Ruta graveolens Linn. (PL) could inhibit pregnancy in 50-60% of rats. The rest of the plants were either inactive or showed insignificant antifertility activity. Anti-implantation activity of some indigenous plants in rats Prakash AO, Saxena V, Shukla S, Tewari RK, Mathur S, Gupta A, Sharma S, Mathur R No
4967454
Antimicrobial activity of Lawsonia inermis L.
PMC547491
5864721
Isolation of securinol A,B, and C from Securinega suffruticosa Rehd. and the structures of securinol A and B. Horii ZI IKEDA M, Tamura Y, Saito S, Kotera K, Iwamoto T. No
5890308
[SThe structure of radiatine, a new alkaloid occurring in Lycoris radiata herb]. Uyeo S, Yamato Y. No
5967157
Occurrence of the alkaloids vittatine and haemanthamine in Lycoris radiata herb. Uyeo S, Kotera K, Okada T, Takagi S, Tsuda Y. No
7400822 The cytotoxic drimane derivatives cinnamodial (1), capsicodendrin (2), a partially characterized tetramer of 1, and cinnamosmolide (3) were obtained from Capsicodendron dinisii (Canellaceae). A new compound, 6 beta-acetoxyisodrimenin (4) and ugandensolide (5) and futronolide (6) were also isolated, but were not cytotoxic. Potential anticancer agents. XVI. Isolation of bicyclofarnesane sesquiterpenoids from Capsicodendron dinisii. Mahmoud II, Kinghorn AD, Cordell GA, Farnsworth NR. No
7528786 From Thalictrum faberi, thalifaberidine [1], a new aporphine-benzylisoquinoline alkaloid, together with four known alkaloids, thalifaramine [2], thalifaricine [3], thalifarazine [4], and thalifaronine [5], were isolated. Thalifaberidine [1] was identified as 6',8-desmethylthalifaberine, and its 1H- and 13C-nmr data were completely assigned through the use of one- and two-dimensional nmr techniques. Thalifaberidine [1], thalifaberine [6], and thalifasine [7] showed cytotoxic activity against several human cancer cell lines, as well as antimalarial activity. Thalifaberidine, a cytotoxic aporphine-benzylisoquinoline alkaloid from Thalictrum faberi. Lin LZ, Hu SF, Zaw K, Angerhofer CK, Chai H, Pezzuto JM, Cordell GA, Lin J, Zheng DM. No
7623031 Asimicin and a new cytotoxic Annonaceous acetogenin, venezenin [1], were isolated from the bark of Xylopia aromatica by bioactivity-directed fractionation using lethality to brine shrimp. Compound 1 represents an unusual type of C37 Annonaceous acetogenin, lacking either tetrahydrofuran (THF) or epoxide rings and possessing a double bond located two methylenes away from a vicinal diol in the hydrocarbon chain. The structure of 1 was elucidated by 1H- and 13C-nmr, COSY, single-relayed COSY, and by HMBC techniques, and derivatization. Annomontacin 10-one [6] and 18/21-cis-annomontacin-10-one [7], two semi-synthetic mono-THF acetogenins were prepared from 1. These acetogenins showed cytotoxicity, comparable or superior to adriamycin, against three human solid tumor cell lines. Reduction of the 10-keto of 1 to the racemic OH-10 derivative enhanced the bioactivity, as did the conversion of 1 to 6 and 7. Venezenin [1], like other Annonaceous acetogenins, showed inhibition of oxygen uptake by rat liver mitochondria and demonstrated that the THF ring may not be essential to this mode of action. Venezenin: a new bioactive Annonaceous acetogenin from the bark of Xylopia aromatica. Colman-Saizarbitoria T, Gu ZM, Zhao GX, Zeng L, Kozlowski JF, McLaughlin JL. No
7714532 Xylopien [1] and xylomatenin [2], two new bioactive monotetrahydrofuran Annonaceous acetogenins, have been isolated from an EtOH extract of the bark of Xylopia aromatica, using bioactivity-directed fractionation employing lethality to brine shrimp. These new compounds each have a double bond in the hydrocarbon chain and have been identified as C-23, C-24 dehydro analogs of xylopiacin and xylomaticin. Their structures were elucidated by spectral analyses of the parent compounds and/or simple chemical derivatives. Their absolute stereochemistries have been established by 1H- and 2D nmr experiments utilizing the production of Mosher esters. These acetogenins showed cytotoxic potencies superior to adriamycin against three human solid tumor cell lines. Two new bioactive monotetrahydrofuran Annonaceous acetogenins from the bark of Xylopia aromatica. Colman-Saizarbitoria T, Gu ZM, McLaughlin JL. No
7766057 A novel flavonol glycoside named caohuoside-B was isolated from the aerial parts of Epimedium koreanum, along with a known flavonol glycoside, epimedokoreanoside-I. Their structures were established by spectroscopic methods. The new compound was elucidated as anhydroicaritin 3-O-beta-D-(4,6-O- diacetyl)glucopyranosyl-(1-->3)-alpha-L-(4"-O-acetylrhamnopyranoside+ ++)-7-O-beta- D-glucopyranoside. Flavonol glycosides from Epimedium koreanum. Li WK, Xiao PG, Tu GZ, Ma LB, Zhang RY. No
7769394 Two new norditerpenoid alkaloids, blacknine [1] and blacknidine [2], have been isolated from the whole plants of Delphinium elatum var. "black night" along with six known alkaloids. The structures of the new alkaloids 1 and 2 were derived from their spectroscopic data (1H, 13C, DEPT, COSY, HETCOR, and selective INEPT nmr experiments). The phytochemistry of this plant variety has not been examined previously. Two new norditerpenoid alkaloids from Delphinium elatum var. "black night". Park JC, Desai HK, Pelletier SW. No
7786477 From the roots of Clematis chinensis, two new triterpenoid saponins, named clematichinenoside A and B, were isolated and their structures were elucidated as 3-O-beta-D-ribopyranosyl-(1-3)-alpha-L-rhamnopyranosyl-(1-2)-alpha -L- arabinopyranosyl oleanolic acid 28-O-beta-D-glucopyranosyl ester and 3-O-beta-D-glucopyranosyl-(1-4)-beta-D-ribopyranosyl-(1-3)-alpha-L - rhamnopyranosyl-(1-2)-alpha-L-arabinopyranosyl hederagenin 28-O-alpha-L-rhamnopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-6)- beta-D-glucopyranosyl ester, respectively. In addition oleanolic acid, hederagenin and seven known saponins were obtained. Triterpenoid saponins from Clematis chinensis. Shao B, Qin G, Xu R, Wu H, Ma K. No
7859329 A new xanthone C-glycoside, polygalaxanthone III (1), and a new acylated sugar, tenuifoliside E (2) were isolated from the roots of Polygala tenuifolia. Their structures were characterized as 4-C-[beta-D-apiofuranosyl-(1-->6)-beta-D-glucopyranosyl]-1, 3,6-trihydroxy-7-methoxyxanthone (1) and beta-D-(1-O-acetyl-3-O-feruloyl-6-O-sinapoyl)-fructofuranosy l-alpha-D-(2,4,6- O-triacetyl)glucopyranoside (2), respectively, on the basis of chemical and spectral evidence including two dimensional nuclear magnetic resonance (2D-NMR) studies. Xanthone C-glycoside and acylated sugar from Polygala tenuifolia. Ikeya Y, Sugama K, Maruno M. No
8009999 Two new phenolic compounds (crystal VI and crystal IX) have been isolated from Phyllanthus urinaria L. (Euphorbiaceae). Their structures were determined by analysis of their UV, IR, 1H-NMR, 1H-1H COSY, 13C-1H COSY, long range 13C-1H COSY, DEPT, EIMS and HREIMS spectral data. Crystal VI was determined as methyl brevifolincarboxylate. Crystal IX was elucidated as trimethyl ester dehydrochebulic acid. All of the signals of the 13C-NMR of these two new compounds have been assigned mainly according to DEPT, 13C-1H COSY and long range 13C-1H COSY. Accompanying these two new compounds, 8 known compounds have been isolated. By using chemical reactions, UV, IR, 1H-NMR, MS, their structures were elucidated as n-octadecane(I), beta-sitosterol(III), ellagic acid(IV), daucosterol(V), kaempferol(VII), quercetin(VIII), gallic acid(X) and rutin(XI). Crystal II is an aldehyde, its structure elucidation is in progress. [Chemical studies on the constituents of Phyllanthus urinaria L]. Yao QQ, Zuo CX. No
8021648 Bioactive Annonaceous acetogenins have been isolated from the EtOH extract of the bark of Xylopia aromatica by bioactivity-directed fractionation using lethality to brine shrimp. These acetogenins include xylopianin [1 , xylopiacin [2], and xylomaticin [3], which are three new mono-tetrahydrofuran ring type acetogenins, in addition to the known compounds, annomontacin, gigantetronenin, gigantetrocin A, and annonacin. Compounds 1 and 2 are unusual in having hydroxylation at C-8; 3 has the same functionalities as annonacin but with 37 carbons instead of 35 carbons. The structures were elucidated by spectral analysis of the parent compounds and/or simple chemical derivatives. These acetogenins showed cytotoxicities, comparable to adriamycin, against three human solid tumor cell lines. Bioactive annonaceous acetogenins from the bark of Xylopia aromatica. Colman-Saizarbitoria T, Zambrano J, Ferrigni NR, Gu ZM, Ng JH, Smith DL, McLaughlin JL. No
8030415 Six compounds were isolated from the seeds of Arctium lappa L. One of them is a new lignan named neoarctin B(VI). The structure has been elucidated on the basis of spectral (UV, IR, 1H-NMR, 13C-NMR, DEPT, 2D-NMR and MS) analysis. The other five compounds were identified as daucosterol (I), arctigenin (II), arctiin (III), matairesinol (IV) and lappaol F (V). [Studies on the chemical constituents of Arctium lappa L]. Wang HY, Yang JS. No
8069956 From the air-dried roots of Glycyrrhiza glabra L. (Leguminosae) collected in Xinjiang province, China ("Shinkyo-Kanzo" in Japanese), five new flavonoid compounds named glucoliquiritin apioside (1) (a flavonone bisdesmoside), prenyllicoflavone A (5) (a bisprenylflavone), shinflavone (7) (a prenylated pyranoflavanone), shinpterocarpin (9) and 1-methoxyphaseollin (12) (both pyranopterocarpans), were isolated together with eight known saponins, seven known flavonoid glycosides, and eleven flavonoids. The structures of the new compounds have been elucidated on the basis of their chemical and physicochemical properties. Chemical studies of Chinese licorice-roots. I. Elucidation of five new flavonoid constituents from the roots of Glycyrrhiza glabra L. collected in Xinjiang. Kitagawa I, Chen WZ, Hori K, Harada E, Yasuda N, Yoshikawa M, Ren J. No
8277320 The extract of the aerial parts of Calendula arvensis afforded four new sesquiterpene glycosides 1-4 in addition to three known compounds. The structures of the new compounds were established by high field 1H-nmr spectroscopy. Sesquiterpene glycosides from Calendula arvensis Ahmed AA, Jakupovic J, Mabry TJ No
8302956 A new phenolic glycoside, tinosinen (1), was isolated from the fresh stems of Tinospora sinensis Merr. The structure was established on the basis of acid hydrolysis and spectral data (IR, NMR, mass) as (E)-1-(3-hydroxy-1-propenyl)-3,5-dimethoxyphenyl 4-O-beta-D-apiofuranosyl-(1-->3)-beta-D-glucopyranoside. Studies on the constituents of Tinospora sinensis; I. Separation and structure of the new phenolic glycoside tinosinen. Yonemitsu M1, Fukuda N, Kimura T. No
8441785 The essential oils of the aerial parts of Jasonia candicans and J. montana were analyzed by gas chromatography-mass spectrometry (GC/MS) technique. Of twenty-one components identified in the volatile oil of J. candicans, intermediol was the main constituent. Fifty-eight components were characterized in the essential oil of J. montana. Camphor, borneol, bornyl acetate, chrysanthemol, intermediol, and 1,8-cineole were the major constituents in this oil. The two oils showed antibacterial activity against Bacillus subtilis. They also showed a marked antifungal activity against Trichophyton mentagrophytes, Cryptococcus neoformans, and Candida albicans. Chemical composition and antimicrobial activity of essential oils of Jasonia candicans and J. montana. Hammerschmidt FJ, Clark AM, Soliman FM, el-Kashoury ES, Abd el-Kawy MM, el-Fishawy AM No
8569244 The anti-hyperglycemic effect of 12 edible plants was studied on 27 healthy rabbits, submitted weekly to subcutaneous glucose tolerance tests after gastric administration of water, tolbutamide or a traditional preparation of the plant. Tolbutamide, Cucurbita ficifolia, Phaseolus vulgaris, Opuntia streptacantha, Spinacea oleracea, Cucumis sativus and Cuminum cyminum decrease significantly the area under the glucose tolerance curve and the hyperglycemic peak. Brassica oleracea var. botrytis, Allium cepa and Allium sativum only decrease the hyperglycemic peak. The glycemic decreases caused by Psidium guajava, Brassica oleracea and Lactuca sativa var. romana were not significant (P > .05). The integration of a menu that includes the edible plants with hypoglycemic activity for the control and prevention of diabetes mellitus may be possible and recommendable. Anti-hyperglycemic effect of some edible plants. Roman-Ramos R, Flores-Saenz JL, Alarcon-Aguilar FJ. No
8590641 Bioassay-directed fractionation of a methylene chloride extract of the root bark of Erythrina eriotricha resulted in the isolation of a novel isoflavanone, named eriotrichin B, and the five known pterocarpans, isonorautenol, erybraedin A, erybraedin C, erybraedin D and erybraedin E. The structure of the new compound has been investigated by extensive spectroscopic studies, including 2D NMR and chemical evidence. The in vitro antimicrobial spectrum and potencies of the isolated compounds are also reported. Prenylated isoflavanone from Erythrina eriotricha. Nkengfack AE1, Vardamides JC, Fomum ZT, Meyer M. No
8699181 Three new hetisane-type diterpenoid alkaloids, davisinol (6), 18-benzoyldavisinol (7), and Davisin (9) have been isolated from Delphinium davisii Munz. and their structures established by detailed spectroscopic studies. Accurate 1H- and 13C-NMR assignments have been made for kobusine (8), a related hetisane-type alkaloid, and karakoline (5), a norditerpenoid alkaloid. The known norditerpenoid alkaloids 14-acetylperegrine (4), 6-deacetylperegrine (3), and karakoline (5) and the diterpenoid alkaloids hetisine and hetisinone were also isolated. Diterpenoid alkaloids from Delphinium davisii. Ulubelen A, Desai HK, Srivastava SK, Hart BP, Park JC, Joshi BS, Pelletier SW, Meriçli AH, Meriçli F, Ilarslan R. No
8728716 Seven new triterpenoid saponins were isolated and identified from the aerial parts of Fagonia arabica. They were characterized as 3-O-beta-D-xylopyranosyl(1-->2)-[beta-D-glucopyranosyl(1-->3)]-alpha-L- arabinopyranosyl oleanolic acid 28-O-beta-D-glucopyranoside, 3-O-beta-D-glucopyranosyl(1-->2)-[beta-D-glucopyranosyl(1-->3)]-alpha-L- arabino pyranosyl oleanolic acid 28-O-beta-D-glucopyranoside, 3-O-beta-D-xylopyranosyl(1-->2)-[beta-D-glucopyranosyl (1-->3)]-alpha- L-arabinopyranosyl oleanolic acid, 3-O-beta-D-glucopyranosyl(1-->2)-[beta-D-glucopyranosyl(1-->3)]-alpha-L- arabino pyranosyl oleanolic acid, 3-O-beta-D-xylopyranosyl(1-->2)-[beta-D-glucopyranosyl (1-->3)]-alpha-L-arabinopyranosyl 27-hydroxyoleanolic acid, 28-O-beta-D-glucopyranoside, 3-O-beta-D-xylopyranosyl(1-->2)-[beta-D-glucopyranosyl(1-->3)]-alpha-L- arabinopyranosyl ursolic acid 28-O-beta-D-glucopyranoside and 3-O-beta-D-xylopyranosyl (1-->2)-[beta-D-glucopyranosyl (1-->3)-alpha-L-arabinopyranosyl 27-hydroxyursolic acid 28-O-beta-D-glucopyranoside. The structures of the saponins were established by analyses of their 1H and 13C NMR spectra with the aid of 2D experiments. The two genins, 27-hydroxyoleanolic acid and 27-ursolic acid, are new. Saponins from Fagonia arabica. Miyase T, Melek FR, El-Gindi OD, Abdel-Khalik SM, El-Gindi MR, Haggag MY, Hilal SH. No
8864243 From the aerial parts of Consolida oliveriana (DC) Schröd. a new norditerpenoid alkaloid consolidine (2) has been isolated, in addition to the known alkaloids pubescenine (1), gigactonine, and delsoline and the diterpenoid alkaloid ajaconine (4). The structure of alkaloid 2 was established on the basis of its physical and spectroscopic data including detailed NMR studies. A detailed NMR study on ajaconine (4) resulted in the revision of 11 13C chemical shift assignments. Diterpenoid alkaloids from Consolida oliveriana. Ulubelen A, Desai HK, Hart BP, Joshi BS, Pelletier SW, Meriçli AH, Meriçli F, Ozen HC. No
8904847 A crude MeOH extract of Syzygium aromaticum (clove) exhibited preferential growth-inhibitory activity against Gram-negative anaerobic periodontal oral pathogens, including Porphyromonas gingivalis and Prevotella intermedia. By means of bioassay-directed chromatographic fractionation, eight active compounds were isolated from this extract and were identified as 5,7-dihydroxy-2-methylchromone 8-C-beta-D-glucopyranoside, biflorin, kaempferol, rhamnocitrin, myricetin, gallic acid, ellagic acid, and oleanolic acid, based on spectroscopic evidence. The antibacterial activity of these pure compounds was determined against Streptococcus mutans, Actinomyces viscosus, P. gingivalis, and P. intermedia. The flavones, kaempferol and myricetin, demonstrated potent growth-inhibitory activity against the periodontal pathogens P. gingivalis and P. intermedia. Compounds from Syzygium aromaticum possessing growth inhibitory activity against oral pathogens. Cai L, Wu CD. No
8966192 Crude ethanolic extract of Lawsonia inermis L. (0.25-2.0 g/kg) produced significant and dose-dependent anti-inflammatory, analgesic, and antipyretic effects in rats. The extract also produced significant increases in pentobarbitone-induced sleeping time. Using a liquid-liquid extraction procedure, the extract was fractionated into chloroform, butanol, and water fractions, and these were tested for the above activities. The butanol and chloroform fractions showed more potent anti-inflammatory, analgesic, and antipyretic effects than the crude extracts, while the aqueous extract showed significantly less effect. As compared with the other extracts, the butanolic extract (500 mg/kg) was the most effective in the analgesic test. From the chloroform extract, a pure compound was isolated and identified, using chromatographic and spectroscopic techniques, as 2-hydroxy-1,4-naphthaquinone (lawsone). The isolated compound was found to possess significant anti-inflammatory, analgesic, and antipyretic activity. It potentiated significantly the pentobarbitone-induced sleeping time. The anti-inflammatory effect of lawsone (500 mg/kg) was not significantly different from that of the reference drug phenylbutazone (100 mg/kg). Anti-inflammatory, antipyretic, and analgesic effects of Lawsonia inermis L. (henna) in rats Ali BH, Bashir AK, Tanira MO No
9207986 Extracts of dried flowers from Calendula officinalis were examined for their ability to inhibit the human immunodeficiency virus type 1 (HIV-1) replication. Both organic and aqueous extracts were relatively nontoxic to human lymphocytic Molt-4 cells, but only the organic one exhibited potent anti-HIV activity in an in vitro MTT/tetrazolium-based assay. In addition, in the presence of the organic extract (500 micrograms/mL), the uninfected Molt-4 cells were completely protected for up to 24 h from fusion and subsequent death, caused by cocultivation with persistently infected U-937/HIV-1 cells. It was also found that the organic extract from Calendula officinalis flowers caused a significant dose- and time-dependent reduction of HIV-1 reverse transcription (RT) activity. An 85% RT inhibition was achieved after a 30 min treatment of partially purified enzyme in a cell-free system. These results suggested that organic extract of flowers from Calendula officinalis possesses anti-HIV properties of therapeutic interest. Anti-HIV activity of extracts from Calendula officinalis flowers. Kalvatchev Z, Walder R, Garzaro D. No
9214709 The glycosidic fraction from the dried roots of Panax notoginseng (Burk.) F.H. Chen was found to show protective effect on liver injury induced by D-galactosamine and lipopolysaccharide. From the glycosidic fraction with hepatoprotective effect, nine new dammarane-type triterpene oligoglycosides, notoginsenosides-A, -B, -C, -D, -E, -G, -H, -I, and -J and an acetylenic fatty acid glycoside, notoginsenic acid beta-sophoroside, were isolated together with fourteen known dammarane-type triterpene oligoglycosides. The structures of notoginsenosides-A, -B, -C and -D were determined on the basis of chemical and physicochemical evidence, which included the chemical correlation with ginsenoside-Rb1 using photosensitized oxygenation, as follows: notoginsenoside A; 3-O-[beta-D-glucopyranosyl (1-->2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranosyl] 3 beta, 12 beta,20(S),25-tetrahydroxydammar -23-ene; B; 3-O-[beta-D-glucopyranosyl (1-->2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl (1-->6)-beta-D- glucopyranosyl] 3 beta, 12 beta,20(S)-trihydroxydammar-25-en-24-one, C; 3-O-[beta-D-glucopyranosyl (1-->2)-beta-D-glucopyranosyl]-20-O-[beta-D -glucopyranosyl (1-->6)-beta-D-glucopyranosyl] 3 beta,12 beta,20(S)- trihydroxy-24 zeta-hydroperoxydammar-25-ene, and D; 3-O-[beta-D-xylopyranosyl (1-->2)-beta-D-glucopyranosyl (1-->2)-beta-D-glucopyranosyl]-20-O-[beta-D-xylopyranosyl (1-->6)-beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranosyl]20(S)-protopanaxadiol, respectively. Bioactive saponins and glycosides. VIII. Notoginseng (1): new dammarane-type triterpene oligoglycosides, notoginsenosides-A, -B, -C, and -D, from the dried root of Panax notoginseng (Burk.) F.H. Chen. Yoshikawa M1, Murakami T, Ueno T, Yashiro K, Hirokawa N, Murakami N, Yamahara J, Matsuda H, Saijoh R, Tanaka O. No
9324003 Hypoglycaemic, antihyperglycaemic and hypolipidemic activities of the aqueous and 50% ethanolic extracts of Caesalpinia bonducella Fleming (Leguminosae) seeds were studied in normal and streptozotocin (SZ)-diabetic rats. In normal rats, both the extracts exhibited hypoglycaemic activity as early as 4 h after administration at a lower dose of 100 mg/kg. The hypoglycaemia produced by the aqueous extract was of prolonged duration as compared to ethanolic extract. In diabetic rats, both the extracts produced significant (P < 0.01) antihyperglycaemic effect from day 5 onwards. Aqueous extract also exhibited antihypercholesterolemic and antihypertriglyceridemic effects in SZ-diabetic rats. These results suggest that C. bonducella seeds possess an antidiabetic principle and can be useful for treatment of diabetes. Further studies are warranted to fractionate the active principle and to find out its exact mechanism of action Hypoglycaemic, antihyperglycaemic and hypolipidemic activities of Caesalpinia bonducella seeds in rats. Sharma SR, Dwivedi SK, Swarup D. No
9388937 Six compounds were isolated from the roots of Ampelopsis sinica and identified as lupeol, beta-sitosterol, daucosterol, catechin, sucrose and palmitic acid on the basis of spectroscopic. Lupeol is found in Ampelopsis genus for the rirst time. [Chemical constituents in roots of Ampelopsis sinica (Miq.) W.T. Wang]. Chen K. No
9542168 Three new meroterpenoid naphthoquinones, the known cordiaquinone B and a new naphthoxirene have been isolated from the roots of Cordia linnaei. Their structures were established by spectrometric methods including EI, D/CI and FAB mass spectrometry, 1H, 13C and 2D NMR experiments. The naphthoquinones showed activity against Cladosporium cucumerinum, Candida albicans and the larvae of the yellow fever-transmitting mosquito Aedes aegypti, while the naphthoxirene derivative was found to be inactive in the same bioassays. Antifungal and larvicidal meroterpenoid naphthoquinones and a naphthoxirene from the roots of Cordia linnaei. Ioset JR, Marston A, Gupta MP, Hostettmann K. No
9582007 Extracts of 40 different plant species used in the traditional medicine of the Australian Aboriginal people have been investigated for antiviral activity. The extracts have been tested for activity against one DNA virus, human cytomegalovirus (HCMV) and two RNA viruses, Ross River virus (RRV) and poliovirus type 1, at non-cytotoxic concentrations. The most active extracts were the aerial parts of Pterocaulon sphacelatum (Asteraceae) and roots of Dianella longifolia var. grandis (Liliaceae), which inhibited poliovirus at concentrations of 52 and 250 microg/ml, respectively. The extracts of Euphorbia australis (Euphorbiaceae) and Scaevola spinescens (Goodeniaceae) were the most active against HCMV. Extracts of Eremophila latrobei subsp. glabra (Myoporaceae) and Pittosporum phylliraeoides var. microcarpa (Pittosporaceae) exhibited antiviral activity against RRV. Screening of Australian medicinal plants for antiviral activity. Semple SJ, Reynolds GD, O'Leary MC, Flower RL. No
9584394 Five new linear acetylenic compounds, namely, pentadeca-6,8,10-triynoic acid (1), octadeca-8,10,12-triynoic acid (2), trans-pentadec-10-en-6,8-diynoic acid (3), cis-hexadec-11-en-7,9-diynoic acid (4), and cis-octadec-12-en-7,9-diynoic acid (5), were isolated from the bark of Heisteria acuminata by bioassay-guided fractionation, using cyclooxygenase (COX) and 5-lipoxygenase (5-LO) assays as models for antiinflammatory activity. The structures of compounds 1-5 were established by NMR, MS, IR, and Raman spectroscopy. These isolated compounds were found to be potent inhibitors of COX. Compounds 4 and 5 were the most potent inhibitors of 5-LO, whereas the other compounds only showed a weak inhibition at the same concentration. New acetylenes isolated from the bark of Heisteria acuminata. Kraus CM, Neszmélyi A, Holly S, Wiedemann B, Nenninger A, Torssell KB, Bohlin L, Wagner H No
9834164 Three new flavonoids (1-3) and 11 known compounds (genistein, isoprunetin, wighteone, laburnetin, alpinumisoflavone, genistin, genistein 8-C-glucoside, apigenin, isokaempferide, licoflavone C, and D-pinitol) were isolated from the aerial parts of Genista ephedroides. The structures of the new compounds were established as hydroxyalpinumisoflavone (1) [4', 5-dihydroxy-2"-methyl-2"-hydroxymethylpyrano(5",6":6,7)isoflavone], ephedroidin (2) [4',5, 7-trihydroxy-8-(2-hydroxy-3-methyl-3-butenyl)flavone], and genisteone (3) (7-O-glucosylwighteone) by means of spectroscopic methods. Flavonoids from genista ephedroides Pistelli L1, Bertoli A, Giachi I I, Manunta A. No
9868163 A new prenylated flavonol, sophoflavescenol (1), together with five known flavonoids, kurarinol, kushenol K, kushenol H, trifolirhizin, and kuraidin, were isolated from the roots of Sophora flavescens. The structure of 1 was determined by spectroscopic analysis. Among the five known flavonoids, kurarinol, kushenol K, and kushenol H showed weak antiviral activity against Herpes simplex virus types I and II. A new prenylated flavonol from the roots of Sophora flavescens. Woo ER, Kwak JH, Kim HJ, Park H. No
9904502 In present study, we investigated hypoglycemic and antihyperglycemic potential of five extracts (water, ethanol, methanol, hexane, and chloroform) of four plants (i.e., seeds of Eugenia jambolana, fruits of Momordica charantia, leaves of Gymnema sylvestre, and seeds of Trigonella foenum graecum) alone and/or in combination with glimepiride in rats. Ethanol extract of E. jambolana, water extract of M. charantia, ethanol extract of G. sylvestre, and water extract of T. graecum exhibited highest hypoglycemic and antihyperglycemic activity (most active) in rats among all the extracts, while hexane extracts exhibited least activities. Most active extracts were further studied to dose-dependent (200, 100, and 50 mg/kg body weight (bw)) hypoglycemic and antihyperglycemic effects alone and in combination with glimepiride (20, 10, and 5 mg/kg bw). The combination of most active extracts (200 mg/kg bw) and lower dose of glimepiride (5 mg/kg bw) showed safer and potent hypoglycemic as well as antihyperglycemic activities without creating severe hypoglycemia in normal rats, while higher doses (200 mg/kg bw of most active extracts, and 10 and 20 mg/kg bw of glimepiride) were generated lethal hypoglycemia in normal rats. From this study, it may be concluded that the ethanol extract of E. jambolana seeds, water extract of M. charantia fruits, ethanol extract of G. sylvestre leaves, and water extract of T. graecum seeds have higher hypoglycemic and antihyperglycemic potential and may use as complementary medicine to treat the diabetic population by significantly reducing dose of standard drugs. Complementary and comparative study on hypoglycemic and antihyperglycemic activity of various extracts of Eugenia jambolana seed, Momordica charantia fruits, Gymnema sylvestre, and Trigonella foenum graecum seeds in rats. Yadav M, Lavania A, Tomar R, Prasad GB, Jain S, Yadav H. No
10190189 Folk medicinal plants are potential sources of useful therapeutic compounds including some with antiviral activities. Extracts prepared from 10 South American medicinal plants (Baccharis trinervis, Baccharis teindalensis, Eupatorium articulatum, Eupatorium glutinosum, Tagetes pusilla, Neurolaena lobata, Conyza floribunda, Phytolacca bogotensis, Phytolacca rivinoides and Heisteria acuminata) were screened for in vitro antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. The most potent inhibition was observed with an aqueous extract of B. trinervis, which inhibited HSV-1 replication by 100% at 50-200 micrograms/mL, without showing cytotoxic effects. Good activities were also found with the ethanol extract of H. acuminata and the aqueous extract of E. articulatum, which exhibited antiviral effects against both DNA and RNA viruses (HSV-1 and VSV, respectively) at 125-250 micrograms/mL. The aqueous extracts of T. pusilla (100-250 micrograms/mL), B. teindalensis (50-125 micrograms/mL) and E. glutinosum (50-125 micrograms/mL) also inhibited the replication of VSV, but none of the extracts tested had any effect on poliovirus replication. Antiviral activity of some South American medicinal plants. Abad MJ, Bermejo P, Sanchez Palomino S, Chiriboga X, Carrasco L. No
10192967 From the roots of Thalictrum faberi, six new phenolic aporphine-benzylisoquinoline alkaloids, 3-hydroxy-6'-desmethyl-9-O-methylthalifaboramine (1), 3-hydroxythalifaboramine (2), 6'-desmethylthalifaboramine (3); 3,5'-dihydroxythalifaboramine (4), 5'-hydroxythalifaboramine (5) and 3-hydroxy-6'-desmethylthalifaboramine (6) were isolated. Their structures were established through the use of one- and two-dimensional NMR techniques. All of the tested alkaloids showed potent cytotoxic and antimalarial activities. Phenolic aporphine-benzylisoquinoline alkaloids from Thalictrum faberi. Lin LZ, Hu SF, Chu M, Chan TM, Chai H, Angerhofer CK, Pezzuto JM, Cordell GA. No
10323492 Ethanolic and aqueous extracts of seven plant species used in the traditional medicine of Bolivia have been tested for their antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV), and poliovirus type 1. The aqueous extracts of most of the species investigated showed antiviral activity. Two of these plants-namely, Satureja boliviana and Baccharis genistelloides-were active against two different viruses-HSV-1 and VSV. Antiviral activity of Bolivian plant extracts. Abad MJ, Bermejo P, Gonzales E, Iglesias I, Irurzun A, Carrasco L. No
10425113 Two new lignans (R,Z)-3-(3,4-dimethoxybenzyl)-2-(3, 4-dimethoxybenzylidene)-gamma-butyrolactone (1) and (2R, 3S)-2-acetoxy-2,3-bis(3,4-dimethoxybenzyl)-gamma-butyrolactone (2), and four new polyacetylenes, (2Z,8E,10E)-heptadeca-2,8,10-triene-4, 6-diyn-1-ol (3), (2E,9Z)-octadeca-2,9-diene-4,6-diyne-1,18-diol (4), (2E,9Z)-18-hydroxy-octadeca-2,9-diene-4,6-diyn-1-yl acetate (5), and (2E,9Z)-1-hydroxy-octadeca-2,9-diene-4,6-diyn-18-yl acetate (6), were isolated and identified from the hexane extract of aerial parts of Bupleurum acutifolium Boiss. Their structures were elucidated by NMR and MS analyses. Lignans and polyacetylenes from bupleurum acutifolium Barrero AF, Herrador MM, Akssira M, Arteaga P, Romera JL. No
10425123 Six new sucrose esters, sibiricoses A(1)-A(6) (1-6), two new xanthone C-glycosides, sibiricaxanthones A (7) and B (8), and a new acetophenone glycoside, sibiricaphenone (9), were isolated from the roots of Polygala sibirica together with six known glycosides. The structures of new compounds were elucidated on the basis of chemical and spectroscopic evidence. The structure of the known xanthone glycoside, polygalaxanthone III (10), was revised. Sucrose esters and xanthone C-glycosides from the roots of Polygala sibirica. Miyase T, Noguchi H, Chen XM. No
10425134 Two new spirostanol saponins, tuberosides D and E, have been isolated from the seeds of Allium tuberosum. On the basis of spectral data and chemical reactions, their structures were established as (25S)-5alpha-spirostane-2alpha,3beta-diol 3-O-alpha-L-rhamnopyranosyl-(1-->2)-O-[alpha-L-rhamnopyranosyl-(1-->4 )]-O-beta-D-glucopyranoside and (25S)-5alpha-spirostan-2alpha, 3beta-diol 3-O-beta-D-glucopyranosyl-(1-->2)-O-[alpha-L-rhamnopyranosyl-(1-->4)] -O-beta-D-glucopyranoside, respectively. Two new spirostanol saponins from Allium tuberosum. Sang S1, Lao A, Wang H, Chen Z. No
10425149 From the leaves of Alangium platanifolium var. platanifolium collected in Fukuoka Prefecture, Japan, three megastigmane diglycosides (1-3) were isolated, along with two known compounds, benzyl alcohol 7-O-beta-D-(6'-O-beta-D-xylopyranosyl)glucopyranoside and Z-hex-3-en-1-ol 1-O-beta-D-glucopyranoside. The structures of the new compounds, named platanionosides A (1), B (2), and C (3) were elucidated by spectroscopic evidence to be 3S,5R,6R,9R, 7E-megastigma-3,9-diol 3,9-di-O-beta-D-glucopyranoside, 3R,9R, 7E-megastigma-5,7-diene-3,9-diol 3,9-di-O-beta-D-glucopyranoside, and 5R,6S,7E-megastigma-3-on-7-en-9-ol 9-O-beta-D-(6'-O-beta-D-xylopyranosyl)glucopyranoside, respectively. Platanionosides A-C, megastigmane diglycosides from the leaves of Alangium platanifolium. Tamaki A1, Otsuka H, Ide T. No
10433484 Antibacterial activity of aqueous residues of 16 different ethnomedicinal plants have been studied. The effect of the aqueous extract at two different weights of plant residues, 30 and 40 mg, were tested against three gram positive bacteria and seven gram negative bacteria by the filter paper disc diffusion method. Among the tested plants, Cleome gynandropsis and Ageratum conyzoides showed a significant control of the growth of Alkaligens viscolactis, Klebsiella aerogenas, Bacillus cerues and Streptococcus pyogens. The maximum inhibitions were observed in Tridax procumbens, Cleome viscosa, Acalypha indica and Boerhaavia erecta against Aeromonas hydrophilla and Bacillus cerues. Preliminary screening of ethnomedicinal plants from India. Perumal Samy R, Ignacimuthu S, Raja DP. No
10444859 Two new triterpenoid saponins, 3-O-{[beta-D-glucopyranosyl-(1-->2)]-[alpha-L-arabinopyranosyl-(1- ->3)]- alpha-L-arabinopyranosyl}-ursolic acid-28-O-[beta-D-glucopyranosyl] ester (indicasaponin A), 3-O-{[beta-D-glucopyranosyl-(1-->2)]-[alpha-L-arabinopyranosyl-(1- ->3)]- alpha-L-arabinopyranosyl}-oleanolic acid-28-O-[beta-D-glucopyranosyl] ester (indicasaponin B) and two known triterpenoid saponins, 3-O-[beta-D-glucopyranosyl-(1-->3)-alpha-L-arabinopyranosyl]-ur solic acid-28-O-[beta-D-glucopyranosyl] ester, 3-O-[beta-D-glucopyranosyl-(1-->3)-alpha-L-arabinopyranosyl]-olean olic acid-28-O-[beta-D-glucopyranosyl] ester have been isolated from Fagonia indica. The structures were determined primarily by NMR spectroscopy. The assignment of NMR signals was performed by means of 1H-1H COSY, NOESY, ROESY, TOCSY, HMQC and HMBC experiments. Triterpenoid saponins from Fagonia indica Shaker KH, Bernhardt M, Elgamal MH, Seifert K No
10543899 Guided by brine shrimp toxicity and human tumor cell toxicity, fractionation of the alcoholic extract from the stem bark of Goniothalamus marcanii led to the isolation of four new 1-azaanthraquinones: marcanines B (3), C (4), D (5), and E (6), along with two known derivatives: marcanine A and dielsiquinone. A new 5-hydroxy-3-amino-2-aceto-1,4-naphthoquinone (7), a possible 1-azaanthraquinone biosynthetic precursor, was also isolated. The structures of the compounds were elucidated by spectroscopic analyses, mainly 1D and 2D NMR techniques ((1)H, (13)C, NOEDS, COSY, HMQC, and HMBC), as well as comparison with literature data. All the compounds except 6 were evaluated for cytotoxic activity. They exhibited significant cytotoxicity against several human tumor cell lines, A-549, HT-29, MCF7, RPMI, and U251 with the ED(50) in the range of 0.04-3.03 microM. New cytotoxic 1-azaanthraquinones and 3-aminonaphthoquinone from the stem bark of Goniothalamus marcanii. Soonthornchareonnon N, Suwanborirux K, Bavovada R, Patarapanich C, Cassady JM. No
10579863 An investigation of the seeds of Swietenia macrophylla and S. aubrevilleana (Meliaceae) is reported. Three new compounds, augustineolide (1) and 3beta,6-dihydroxydihydrocarapin (2) from S. macrophylla and 6-acetoxyhumilinolide C (3) from S. aubrevilleana were isolated and characterized along with fifteen known compounds. Four of the compounds were subjected to an antifeedant bioassay on the final instar larvae of Spodoptera frugiperda. The antifeedant activity was comparable to that of bicyclononanolides previously tested. Limonoids from Swietenia macrophylla and S. aubrevilleana. Mootoo BS, Ali A, Motilal R, Pingal R, Ramlal A, Khan A, Reynolds WF, McLean S. No
10624899 Holarrhena pubescens (syn. H. antidysenterica) (L.) WALL. stem bark was tested for antibacterial efficacy against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus faecalis, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa using the microdilution broth method as well as the disc diffusion method. The crude methanolic extract was active against all tested bacteria. Further chemical fractionation indicated that the antibacterial activity was mainly associated with the alkaloids. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for the crude extract, the total alkaloids and the neutral fraction using microdilution broth method. The results were compared with reference antibiotics. The total alkaloids showed remarkable activity against S. aureus (MIC = 95 microg/ml). Antibacterial steroid alkaloids from the stem bark of Holarrhena pubescens. Chakraborty A, Brantner AH. No
10650068 From the roots of Delphinium stapeliosum three new norditerpenoid alkaloids, 14-demethyltuguaconitine (1), 14-deacetyl-14-isobutyrylnudicauline (2), and 14-deacetyl-14-isobutyrylajadine (3), and nine known norditerpenoid alkaloids, delbonine (4), methyllycaconitine (5), 14-deacetylnudicauline (6), ajacine (7), deltatsine (8), delcosine (9), 14-deacetylajadine (10), nudicauline (11), and ajadine (12), were isolated. Structure elucidation and identification were based on NMR and mass spectra. Norditerpenoid alkaloids from the roots of Delphinium stapeliosum. Shrestha PM, Katz A. No
10656408 Two nitro compounds, 2-(4-methoxyphenyl)-1-nitroethane named as lysichitalexin and 2-(4-hydroxyphenyl)-1-nitroethane were isolated as stress metabolites from the leaves of Lysichitum americanum Hultén and St. John treated with cupric chloride. Their structures were determined by spectroscopic methods and chemical reactions. The former compound showed antifungal activities against Fusarium oxysporum and Cladosporium herbarum. Both compounds were isolated for the first time from this species and the former was isolated from natural sources for the first time. This is the first report on stress metabolites from a member of the Araceae. Antifungal nitro compounds from skunk cabbage (Lysichitum americanum) leaves treated with cupric chloride. Hanawa F, Tahara S, Towers GH. No
10661881 The crude extract from the bark and seeds of Khaya grandifoliola was active in vitro against Plasmodium falciparum with an IC50 value of 13.23 microg/ml. The extract was purified to obtain seven limonoids--methylangolensate (1), 6-methylhydroxyangolensate (2), gedunin (3), 7-deacetylkhivorin (5), 1-deacetylkhivorin (6), swietenolide (7), 6-acetylswietenolide (8)--and one flavonoid, catechin (4). Five limonoids (1, 3, 5, 6, 8) were active with IC50 values between 1.25 and 9.63 microg/ml. Catechin was practically devoid of activity. The most active limonoid, gedunin, exhibited an additive effect when combined with chloroquine. In vitro antimalarial activity of limonoids from Khaya grandifoliola C.D.C. (Meliaceae). Bickii J, Njifutie N, Foyere JA, Basco LK, Ringwald P. No
10685103 In the present study we analysed the possible antiviral effect on dengue viruses of different flavonoids extracted and identified at the Chemistry Institute, UNAM, from the Mexican plants Tephrosia madrensis, Tephrosia viridiflora and Tephrosia crassifolia. The flavonoids glabranine and 7-O-methyl-glabranine presented 70% inhibition on the dengue virus at a concentration of 25 microM, while methyl-hildgardtol A, hildgardtol A and elongatine had no effect on viral growth. Our results show that glabranine and 7-O-methyl-glabranine isolated from Tephrosia s.p. exert a dose-dependent inhibitory effect in vitro on the dengue virus. Antiviral effect of flavonoids on the dengue virus. Sánchez I, Gómez-Garibay F, Taboada J, Ruiz BH. No
10724189 In addition to the known cordiaquinones A and B, two novel meroterpenoid naphthoquinones, named cordiaquinones J and K, have been isolated from the roots of Cordia curassavica. Their structures were elucidated by spectrometric methods including EI, D/CI mass spectrometry, 1H, 13C and 2D-NMR experiments. The four naphthoquinones demonstrated antifungal activities against Cladosporium cucumerinum, Candida albicans and toxic properties against larvae of the yellow fever-transmitting mosquito Aedes aegypti. Antifungal and larvicidal cordiaquinones from the roots of Cordia curassavica. Ioset JR, Marston A, Gupta MP, Hostettmann K. No
10727815 Diterpenes and phenolic acids, including the new compounds 12 beta-hydroxysandaracopimar-15-ene (4) and 2-propionoxy-beta-resorcylic acid (8) have been isolated from Trichilia heudelotti leaves. The methanol extract showed antimicrobial activity concentrated in the ethyl acetate fraction and some of its constituents. Antimicrobial activity of Trichilia heudelotti leaves. Aladesanmi AJ, Odediran SA. No
10727827 Ferrari F, Delle Monache F, Suarez AI, Compagnone RS. Multicaulisin, a new Diels-Alder type adduct from Morus multicaulis. Ferrari F, Delle Monache F, Suarez AI, Compagnone RS. No
10757739 Two new natural products, a phenylpropanoid derivative characterized as 1-(3'-methoxypropanoyl)-2,4,5-trimethoxybenzene (1) and a prenylated hydroquinone, 2-(2Z)-(3-hydroxy-3,7-dimethylocta-2, 6-dienyl)-1,4-benzenediol (2), have been isolated from the root bark of Cordia alliodora. Both compounds exhibited antifungal properties against the phytopathogenic mold Cladosporium cucumerinum. The phenylpropanoid derivative (1), whose structure is closely related to beta-asarone, also demonstrated a marked activity against larvae of the yellow-fever-transmitting mosquito Aedes aegypti. Antifungal and larvicidal compounds from the root bark of Cordia alliodora. Ioset JR, Marston A, Gupta MP, Hostettmann K. No
10783076 From the leaves of Glochidion zeylanicum, six new butenolide glucoside, named glochidionolactones A-F, were isolated along with a known related compound, phyllanthurinolactone. The structures of glochidionolactones A-D and F were elucidated mainly by spectroscopic analyses. The absolute stereochemistry of glochidionolactone E was established by X-ray crystallographic analysis. Glochidionolactones A-F: butenolide glucosides from leaves of Glochidion zeylanicum (GAERTN) A. JUSS. Otsuka H1, Hirata E, Takushi A, Shinzato T, Takeda Y, Bando M, Kido M. No
10785426 Two new diterpenes (1 and 2) were obtained from the leaves of Tinospora rumphii, along with the known compounds tinotufolin D and vitexilactone. The structures of compounds 1 and 2 were established on the basis of spectroscopic studies. Clerodane diterpenes from Tinospora rumphii. Ragasa CY1, Cruz MC, Gula R, Rideout JA. No
10820816 In addition to two known compounds, 5,4'-di-O-methylalpinumisoflavone and cajanin, a new 3-phenylcoumarin metabolite, named indicanine B, and a new isoflavone derivative, named indicanine C, were isolated from the root bark of Erythrina indica. By means of spectroscopic analysis, the structures of the new compounds were characterized as 4-hydroxy-3-(4'-hydroxyphenyl)-5-methoxy-2",2"-dimethylpyrano [5",6":6,7] coumarin and 4'-hydroxy-5-methoxy-2",2"-dimethylpyrano [5",6":6,7] isoflavone, respectively. The 13C-NMR data of cajanin and the in vitro antimicrobial spectrum and potencies of the isolated compounds are also reported. Indicanines B and C, two isoflavonoid derivatives from the root bark of Erythrina indica. Waffo AK1, Azebaze GA, Nkengfack AE, Fomum ZT, Meyer M, Bodo B, van Heerden FR. No
10820820 Two glycosides, tetracentronsides A and B, were isolated from the stem bark of Tetracentron sinense Oliv., along with ten known compounds, beta-sitosterol, lupeol, betulinic acid, oleanolic acid, vanillic aldehyde, vanillic acid, maslinic acid, huazhongilexin, daucosterol and catechin. On the basis of spectral and chemical evidence, tetracentronside A and B were identified as 3,4,5-trimethoxyphenyl-O-6'-O-vanilloyl-beta-D-glucopyranoside and (8R, 8'R) 9-beta-D-glucopyranosyl dihydrocubebin, respectively. Two glycosides from the stem bark of Tetracentron sinense. Yi JH, Zhang GL, Li BG, Chen YZ. No
10843602 Two new Alangium alkaloids, 1',2'-dehydrotubulosine (1) and alangine (2), were isolated from the dried fruits of Alangium lamarckii along with tubulosine (3), isotubulosine (4), deoxytubulosine, cephaeline, isocephaeline, psychotrine, neocephaeline, 10-O-demethylcephaeline, 2'-N-(1"-deoxy-1" -beta-D-fructopyranosyl)cephaeline, protoemetine, protoemetinol, salsoline, and alangiside. The structures of the new alkaloids (1 and 2) were determined by spectroscopic and chemical means. Two Alangium alkaloids from Alangium lamarckii. Itoh A, Ikuta Y, Tanahashi T, Nagakura N. No
10869220 A new 3-phenylcoumarin, indicanine A (1), has been isolated from the root bark of the African medicinal plant Erythrina indica, together with three known compounds, robustic acid (2), daidzein, and 8-prenyldaidzein. The structure of the new compound was characterized, as 4-hydroxy-5-methoxy-3-(4'-methoxyphenyl)-2" -(1-methylethenyl)dihydrofurano[4",5":6,7]coumarin by means of extensive spectroscopic analyses. The compounds were found to be devoid of in vitro antibacterial activity. Indicanine A, a new 3-phenylcoumarin from root bark of Erythrina indica. Nkengfack AE1, Waffo AK, Azebaze GA, Fomum ZT, Meyer M, Bodo B, van Heerden FR. No
10923841 Two new steroidal saponins, named nigrumnins I and II, together with two known saponins were obtained from the whole plant of Solanum nigrum L. On the basis of spectroscopic analysis (1H-NMR, 13C-NMR, 1H-1H COSY, TOCSY, HMQC, HMBC and FAB-MS), nigrumnin I was established as (25R)-5alpha-spirostan-3beta-ol 3-O-betaD-xylopyranosyl-(1-->3)-[alpha-L-arabinopyranosyl-(1 -->2)]-beta-D- glucopyranosyl-(1-->4)-[alpha-L-rhamnopyranosyl(1-->2)]-beta-D- galactopyranoside (1), and nigrumnin II was elucidated as (25R)-3beta,17alpha-dihydroxy-5alpha-spirostan-1 2-one 3-O-beta-D-xylopyranosyl-(1-->3)-[alpha-L-arabinopyranosyl-(1--> 2)]-beta-D-glucopyranosyl-(1-->4)-[alpha-L-rhamnopyra- nosyl-(1-->2)l-beta-D-galactopyranoside (2). Steroidal oligoglycosides from Solanum nigrum. Ikeda T, Tsumagari H, Nohara T. No
10978199 Two new sucrose esters, arillatoses A (1) and B (2), and four new trisaccharide esters, arillatoses C-F (3-6), were isolated from the roots of Polygala arillata, together with four known sucrose esters, glomeratose E (7) and sibiricoses A(1) (8), A(5) (9), and A(6) (10). The structures of the new compounds were elucidated on the basis of chemical and spectroscopic evidence. Oligosaccharide esters from the roots of Polygala arillata. Kobayashi W, Miyase T, Suzuki S, Noguchi H, Chen XM. No
10978209 Ten new tetrasaccharide multi-esters, watteroses A-J (1-10), and two new xanthone glycosides, wattersiixanthones A (11) and B (12), were isolated from the roots of Polygala wattersii, together with 11 known compounds (10 oligosaccharide multi-esters and a xanthone glycoside). The structures of new compounds were elucidated on the basis of chemical and spectroscopic evidence. Tetrasaccharide multi-esters and xanthone glycosides from the roots of Polygala wattersii. Kobayashi W, Miyase T, Suzuki S, Noguchi H, Chen XM. No
10978212 Three new diterpenoid alkaloids, isoazitine (1), 19-oxodihydroatisine (2), and 22-O-acetyl-19-oxodihydroatisine (3), and eight known alkaloids-azitine (4), dihydroatisine (5), delphinine, neoline, bullatine C (14-acetylneoline), chasmanine, 14-acetylchasmanine, and the quaternary base atisinium chloride (7)-were isolated from the aerial parts of Delphinium staphisagria. Structures of the new alkaloids were established mainly by 1D and 2D NMR spectroscopy, including (1)H COSY, HMQC, HMBC, and ROESY. The (1)H and (13)C NMR data for alkaloids 4 and 5 are also reported. Alkaloids from Delphinium staphisagria. Díaz JG, Ruiz JG, de La Fuente G. No
10985092 Two new isoflavonoids, eryvarin A (1) and eryvarin B (2) were isolated from the wood of Erythrina variegata and their structures were elucidated on the basis of spectroscopic evidence. Two new isoflavonoids from Erythrina variegata. Tanaka H, Etoh H, Shimizu H, Makita T, Tateishi Y. No
11000030 Three new phenol glycosides acylated with (+)-oleuropeic acid, cypellocarpins A (1), B (2), and C (3), along with seven known compounds, were isolated from the dried leaves of Eucalyptus cypellocarpa. Structures of the new compounds were determined on the basis of spectroscopic methods, including 2D NMR experiments and chemical degradation. These new compounds and a known related glucoside (7) showed potent in vitro antitumor-promoting activity in a short-term bioassay evaluating the inhibitory effect on Epstein-Barr virus early antigen activation induced by 12-O-tetradecanoyl phorbol 13-acetate (TPA). These compounds also suppressed an in vivo two-stage carcinogenesis induced with nitric oxide and TPA on mouse skin. Cypellocarpins A-C, phenol glycosides esterified with oleuropeic acid, from Eucalyptus cypellocarpa. Ito H, Koreishi M, Tokuda H, Nishino H, Yoshida T. No
11045440 A new gluco indole alkaloid, 3,4-dehydro-5-carboxystrictosidine, was obtained from Peruvian Uña de Gato (Cat's Claw, original plant: Uncaria tomentosa) together with two known gluco indole alkaloids. This compound was the first example of isolation of a gluco monoterpenoid indole alkaloid having a 3,4-dihydro-beta-carboline ring system from nature. A characteristic feature of the compound was the quick replacement of the methylene hydrogens on C-14 with deuterium that was observed when it was dissolved in CD3OD. We demonstrated a similar proton-deuterium exchange on a model compound, 1-methyl-3,4-dihydro-gamma-carboline, in CD3OD solution. A new gluco indole alkaloid, 3, 4-dehydro-5-carboxystrictosidine, from Peruvian Uña de Gato (Uncaria tomentosa). Kitajima M, Hashimoto K, Yokoya M, Takayama H, Aimi N, Sakai SI. No
11054840 One hundred fifty-two methanol and water extracts of different parts of 71 plants commonly used in Sudanese traditional medicine were screened for their inhibitory effects on hepatitis C virus (HCV) protease (PR) using in vitro assay methods. Thirty-four extracts showed significant inhibitory activity (>/=60% inhibition at 100 microg/mL). Of these, eight extracts, methanol extracts of Acacia nilotica, Boswellia carterii, Embelia schimperi, Quercus infectoria, Trachyspermum ammi and water extracts of Piper cubeba, Q. infectoria and Syzygium aromaticum, were the most active (>/=90% inhibition at 100 microg/mL). From the E. schimperi extract, two benzoquinones, embelin (I) and 5-O-methylembelin (II), were isolated and found as potent HCV-PR inhibitors with IC(50) values of 21 and 46 microM, respectively. Inhibitory activities of derivatives of I against HCV-PR as well as their effects on other serine proteases were also investigated. Inhibitory effects of sudanese medicinal plant extracts on hepatitis C virus (HCV) protease. Hussein G, Miyashiro H, Nakamura N, Hattori M, Kakiuchi N, Shimotohno K. No
11082842 Twelve triterpenoid saponins, including six new, were isolated and identified from the aerial parts of Fagonia glutinosa. The new saponins were characterised as 3-O-[beta-D-glucopyranosyl(1-->2)][beta-D-glucopyranosyl(1-->3)]-alpha-L - arabinopyranosyl-27-hydroxy oleanolic acid 28-O-beta-D-glucopyranosyl ester, 3-O-[beta-D-glucopyranosyl(1-->3)]-alpha-L-arabinopyranosyl ursolic acid, 3-O-alpha-L-arabinopyranosyl ursolic acid 28-O-beta-D-glucopyranosyl ester, 3-O-[beta-D-xylopyranosyl(1-->2)][beta- D-glucopyranosyl(1-->3)]-alpha-L-arabinopyranosyl ursolic acid, 3-O-[beta-D-glucopyranosyl(1-->2)][beta-D- glucopyranosyl(1-->3)]-alpha-L-arabinopyranosyl ursolic acid 28-O-beta-D-glucopyranosyl ester and 3-O-[beta-D-glucopyranosyl(1-->2)][beta-D-glucopyranosyl(1-->3)]-alpha-L - arabinopyranosyl-27-hydroxy ursolic acid 28-O-beta-D-glucopyranosyl ester. The structures of the saponins were established by spectral and chemical evidences. The assignments of the NMR signals were performed by means of HOHAHA, 1H-1H COSY, ROE, HMQC and HMBC experiments. Saponins from Fagonia glutinosa Melek FR, Miyase T, el-Gindy MR, Abdel-Khalik SM, Ghaly NS, el-Kady M No
11087585 Four new xanthones, caledonixanthones A-D (1-4), were isolated from the trunk bark of Calophyllum caledonicum, in addition to 17 known compounds. The structures of 1-4 were determined by means of spectroscopic analysis and chemical derivatization. New xanthones from Calophyllum caledonicum. Morel C, Séraphin D, Oger JM, Litaudon M, Sévenet T, Richomme P, Bruneton J. No
11113996 In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1. Search for antiviral activity in higher plant extracts. Abad MJ, Guerra JA, Bermejo P, Irurzun A, Carrasco L. No
11140606 An unusual biflavonoid named licoagrodin was isolated from the hairy root cultures of Glycyrrhiza glabra (Leguminosae) along with three prenylated retrochalcones, licoagrochalcones B, C, D, a prenylated aurone, licoagroaurone and four known prenylated flavonoids, licochalcone C, kanzonol Y, glyinflanin B and glycyrdione A. From the glycosidic fraction, a isoflavone glycoside, licoagroside A, and a maltol glycoside, licoagroside B were isolated together with four known isoflavone glycosides, two flavone C-glycosides, and three other glycosides. Their structures were elucidated on the basis of spectroscopic evidence Flavonoid constituents from Glycyrrhiza glabra hairy root cultures. Li W, Asada Y, Yoshikawa T. No
11163939 Xylopia frutescens is a tree native to the Brazilian Amazon whose seeds are rich in kaurenoic acid, a diterpene that showed in vitro activity against Trypanosoma cruzi. Aiming to find out alternative sources for kaurenoic acid, the content of some kaurane diterpenes was evaluated in X. aromatica and X. brasiliensis, species occurring in the Cerrado area of Minas Gerais, and also in X. frutescens. A reversed phase HPLC isocratic method was developed and validated to perform the assays. Kaurenoic acid was found to be the most abundant diterpene within the analyzed species, with a 3.16+/-0.97% content in the seeds of X. frutescens, which also presented the highest amount of xylopic acid (1.09+/-0.33%). The highest concentration of 16-alpha-hydroxykauranoic acid (1.96+/-1.58%) was found in the stems of X. aromatica. HPLC quantitation of kaurane diterpenes in Xylopia species. de Melo AC, Cota BB, de Oliveira AB, Braga FC. No
11164504 The essential oil obtained in toto from Santolina insularis was investigated for its antiviral activity on herpes simplex type 1 (HSV-1) and type 2 (HSV-2) in vitro. The IC(50) values, determined by plaque reduction assays, were 0.88 and 0.7 microg/ml for HSV-1 and HSV-2, respectively, while the CC(50) determined by the MTT test on Vero cells was 112 microg/ml, indicating a CC(50)/IC(50) ratio of 127 for HSV-1 and 160 for HSV-2. Results obtained by plaque reduction assays also indicated that the antiviral activity of S. insularis was principally due to direct virucidal effects. Antiviral activity against HSV-1 and HSV-2 was not observed in a post-attachment assay, and attachment assays indicated that virus adsorption was not inhibited. Up to 80% inhibition of HSV-1 was achieved at the concentration of 40 microg/ml by yield reduction assay. Furthermore, reduction of plaque formation assays also showed that S. insularis essential oil inhibits cell-to-cell transmission of both HSV-1 and HSV-2. Inactivation of HSV-1 and HSV-2 and prevention of cell-to-cell virus spread by Santolina insularis essential oil. De Logu A, Loy G, Pellerano ML, Bonsignore L, Schivo ML. No
11167029 Ethanolic extracts of 45 Indian medicinal plants traditionally used in medicine were studied for their antimicrobial activity against certain drug-resistant bacteria and a yeast Candida albicans of clinical origin. Of these, 40 plant extracts showed varied levels of antimicrobial activity against one or more test bacteria. Anticandidal activity was detected in 24 plant extracts. Overall, broad-spectrum antimicrobial activity was observed in 12 plants (L. inermis, Eucalyptus sp., H. antidysentrica, H. indicus, C. equistifolia. T. belerica, T. chebula, E. officinalis, C. sinensis, S. aromaticum and P. granatum). No correlation was observed between susceptibility of test strains with plant extracts and antibiotic resistance behaviour of the microbial strains (Staphylococcus aureus, Salmonella paratyphi, Shigella dysenteriae, Escherichia coli, Bacillus subtilis, Candida albicans). Qualitative phytochemical tests, thin layer chromatography and TLC-bioautography of certain active extracts demonstrated the presence of common phytocompounds in the plant extracts including phenols, tannins and flavonoids as major active constituents. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. Ahmad I, Beg AZ. No
11190388 The dichloromethane extract from the leaves of Guarea macrophylla (Meliaceae) was submitted to adsorption chromatography. Guai-6-en-10beta-ol, isopimara-7,15-dien-2alpha-ol and cycloarta-23,25-dien-3-one were isolated and identified by spectroscopic data, mainly by 1H and 13C NMR analyses; eight other known terpenoids were also isolated. Terpenes from leaves of Guarea macrophylla (Meliaceae). Lago JH, Brochini CB, Roque NF. No
11219815 Four new terpenes including, two sandaracopimaradiene diterpenoids, ent-8(14),15-sandaracopimaradiene-2alpha,18-diol, and ent8(14),15-sandaracopimaradiene-2beta,18-diol, and two lanostane triterpenoids, 23-hydroxy-5alpha-lanosta 7,9(11),24-triene-3-one, and 5alpha-lanosta-7,9(11),24-triene-3alpha,23-diol, were isolated from the methanolic extract prepared from the leaves of G. rhopalocarpa together with the known steroid stigmasterol and the coumarin, scopoletin. The isolates showed weak antiprotozoal activity against Leishmania donovani promastigotes, and Trypanosoma brucei brucei blood stream trypomastigotes, and were devoid of interesting activity towards Plasmodium falciparum. The isolates did not show significant cytotoxic activity against KB cells. Terpenoids from Guarea rhophalocarpa. del Rayo Camacho M, Phillipson JD, Croft SL, Kirby GC, Warhurst DC, Solis PN. No
11243154 Six crystalline substances were isolated from the rhizoma of Pericampylus glaucus and identified as epifriedelinol, melissic acid, palmatic acid, stearic acid, bututic acid and daucosterol. [Chemical constituents of Pericampylus glaucus (Lam.) Merr]. Liang P, Zhou Q, Zhou F. No
11314966 Four isoflavonoids, erysubins C-F, together with ten known compounds were isolated from the roots of Erythrina suberosa var. glabrescences, and their structures were elucidated on the basis of spectroscopic evidence. Erysubin C is an unusual pterocarpan derivative with a formyl group. Erysubins C-F, four isoflavonoids from Erythrina suberosa var. glabrescences. Tanaka H1, Etoh H, Watanabe N, Shimizu H, Ahmad M, Rizwani GH. No
11355766 From the roots of Aconitum sinomontanum, five new norditerpenoid alkaloids, sinomontanitines A (1) and B (2), sinomontanines A (3), B (4) and C (5), were isolated together with the known alkaloids lappaconitine (6) and ranaconitine (7), The structures of the new alkaloids were determined by spectral analysis. Five new norditerpenoid alkaloids from Aconitum sinomontanum. Wang FP, Peng CS, Jian XX, Chen DL. No
11355770 A new diterpenoid alkaloid, racemulotine (1), was isolated from the whole plants of Aconitum racemulosum Franch var. pengzhouense, and its structure was elucidated by 1D- and 2D-NMR spectra. Diterpenoid alkaloids from Aconitum racemulosum Franch var. pengzhouense. Peng CS, Jian XX, Wang FP. No
11374974 Bioassay-guided fractionation of the antiprotozoal extract of Conyza filaginoides led to the isolation of three new flavonol caffeoyl glycosides, namely, kaempferol 3-O-(6' '-O-E-caffeoyl)-beta-D-galactopyranoside (1), isorhamnetin 3-O-(6' '-O-E-caffeoyl)-beta-D-galactopyranoside (2), and quercetin 3-O-(6' '-O-E-caffeoyl)-beta-D-glucopyranoside (3). In addition, seven known compounds, erythrodiol (4), beta-caryophyllene-4,5-alpha-oxide (5), astragalin (6), isoquercitrin (7), nicotiflorin (8), narcissin (9), and rutin (10), were obtained. The structures of the new isolates were elucidated by spectroscopic and chemical methods. Compounds were also assessed for antiamoebic and antigiardial activities, but none was significantly active compared to the standard drugs evaluated. Antiprotozoal activity of the constituents of Conyza filaginoides. Calzada F, Cedillo-Rivera R, Mata R. No
11412954 A new alkaloid identified as 3beta,6beta-ditigloyloxynortropane as 3beta,7beta-ditigloyloxynortropane, 4-hydroxyhygrinic acid, methylecgonidine and tropacocaine have been isolated from the leaves of Erythroxylum argentinum. The new structure was established by means of spectroscopic techniques. Four other species E. deciduum, E. microphyllum, E. pelleterianum and E. cuneifolium collected in the state of Rio Grande do Sul were screened for methylecgonidine and tropacocaine by CG/MS. Two of these plants contain tropacocaine and two contain methylecgonidine. GC/MS analysis of all 5 species for cocaine proved fruitless. The chemotaxonomic significance of these results is discussed. Alkaloids of Erythroxylum (Erythroxylaceae) species from Southern Brazil. Zuanazzi JA, Tremea V, Limberger RP, Sobral M, Henriques AT. No
11421727 Five new prenylated tricyclic and three new bicyclic acylphloroglucinol derivatives have been isolated by bioactivity-guided fractionation of the petroleum ether extract of the dried aerial parts of Hypericum papuanum. The tricyclic compounds (1--5) were named papuaforins A--E. The bicyclic compounds were isolated together with their corresponding tautomers and were named hyperguinones A and B (6/6a,7/7a) and hyperpapuanone (8/8a), respectively. Their structures were elucidated on the basis of extensive 1D and 2D NMR experiments, as well as mass spectrometry. Furthermore, the cytotoxicity toward KB nasopharyngeal carcinoma cells and the antibacterial activity of the isolated compounds were determined. New prenylated bi- and tricyclic phloroglucinol derivatives from Hypericum papuanum. Winkelmann K, Heilmann J, Zerbe O, Rali T, Sticher O. No
11421729 Besides the known compounds longistylines C (1), D (2), and 3,5-dimethoxystilbene (5), five new prenylated stilbenes, named chiricanines A--E (3, 4, 6--8), have been isolated from the root bark of Lonchocarpus chiricanus. Their structures were resolved on the basis of spectrometric methods including (1)H, (13)C, and 2D NMR experiments and mass spectrometry. Compound 3 was the only prenylated stilbene to demonstrate antifungal effects against Cladosporium cucumerinum. Four of the isolated compounds showed toxic properties against larvae of the yellow fever-transmitting mosquito Aedes aegypti. Compound 5 was found to be as potent as rotenone in larvicidal dilution tests. Five new prenylated stilbenes from the root bark of Lonchocarpus chiricanus. Ioset JR, Marston A, Gupta MP, Hostettmann K. No
11421732 Fourteen new sesquiterpenes of the drimane series dendocarbins A--N (1--14) were obtained from ethanol extracts of the Japanese nudibranch Dendrodoris carbunculosa, together with two known compounds, isodrimeninol (15) and 11-epivaldiviolide (16). All structures were elucidated mainly from spectral data, and most of these sesquiterpenes were found to exhibit cytotoxicity. In addition, isodrimeninol (15), the major sesquiterpene of this animal, was found to have a sharp peppery taste Dendocarbins A--N, new drimane sesquiterpenes from the nudibranch Dendrodoris carbunculosa. Sakio Y1, Hirano YJ, Hayashi M, Komiyama K, Ishibashi M. No
11421761 Investigation of an ethanolic extract of the fresh flowers of Hippeastrum vittatum yielded the new alkaloids vittacarboline (1) and O-methylismine (3), together with the known compound ismine (2). The structures of 1--3 were established by spectroscopic methods including one- and two-dimensional NMR and mass spectrometry. Alkaloids of the flowers of Hippeastrum vittatum. Youssef DT. No
11429249 Analysis of the polar fractions of an EtOH extract obtained from the bark of the African medicinal plant Fagara macrophylla led to the isolation and identification of the alkaloids oblongine (6), tembetarine (7) and magnoflorine (8) and the flavonoid hesperidin (9). These compounds, together with other metabolites (1--5) previously isolated from F. macrophylla, were tested for antifeedant activity in a binary-choice bioassay. The acridone alkaloid xanthoxoline (4) was found to have a potent antifeedant activity against larvae of both Spodoptera frugiperda and S. littoralis. 1-Hydroxy-3-methoxy-N-methyl-acridone (2), arborinine (3), tembetarine (7) and magnoflorine (8) were antifeedant against S. frugiperda. Antifeedant constituents from Fagara macrophylla. Tringali C1, Spatafora C, Calì V, Simmonds MS. No
11456091 Baenzigeroside B, a new rearranged clerodane diterpene glucoside, was isolated from the stems of Tinospora baenzigeri. The aglycone of baenzigeroside B, baenzigeride B (isolated as its acetate), was found together with baenzigeride A, baenzigerosides A and B in the leaves of the same plant. Baenzigeride B and baenzigeroside B are the first examples of a new class of rearranged clerodane diterpenes. The possible biogenesis of the compounds is discussed. New rearranged clerodane diterpenes from Tinospora baenzigeri. Tuntiwachwuttikul P1, Taylor WC. No
11456105 From the leaves of Glochidion zeylanicum collected in Okinawa, four flavanol glucosides, named as glochiflavanosides A-D were isolated along with known flavone C-glucosides, vitexin and isoorientin. Their structures were elucidated by spectroscopic analyses. Glochiflavanosides A-D: flavanol glucosides from the leaves of Glochidion zeylanicum (Gaertn) A. Juss. Otsuka H1, Hirata I, Shinzato T, Takeda Y. No
11515588 Following the characterization of hypoglycemic, gastric emptying inhibitory, and gastroprotective principles and the structure elucidation of calendasaponins A, B, C, and D, two new ionone glucosides (officinosides A and B), and two sesquiterpene oligoglycosides (officinosides C and D), were isolated from the flowers of Egyptian Calendula officinalis. The structures of the officinosides were elucidated on the basis of chemical and physicochemical evidence. Medicinal flowers. IV. Marigold. (2): Structures of new ionone and sesquiterpene glycosides from Egyptian Calendula officinalis. Marukami T, Kishi A, Yoshikawa M No
11575954 Nine new xanthones, parvixanthones A-I (1-9), isolated from the dried bark of Garcinia parvifolia, were found to have a common 1,3,6,7-oxygenated pattern for their xanthone nucleus, but various oxygenated isoprenyl or geranyl substituent groups. The structures were determined by spectroscopic methods. Xanthones from Garcinia parvifolia. Xu YJ, Lai YH, Imiyabir Z, Goh SH. No
11596235 Six crystalline substances were isolated from the stem and root of Syzygium buxifolium and identified as friedelin, beta-sitosterol, ursolic acid, pomolic acid, oleanolic acid and beta-daucosterol. [Chemical constituents of the stem and root of Syzygium buxifolium Hook. Et Arn]. Zhou F, Liang P, Zhou Q, Qin Z. No
11600021 Three new spirostanol saponins have been isolated from the seeds of Allium tuberosum. On the basis of acid hydrolysis and comprehensive spectroscopic analysis, their structures were established as tuberoside J, (25R)-5alpha-spirostan-2alpha,3beta,27-triol 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside; tuberoside K, (25R)-5alpha-spirostan-2alpha,3beta,27-triol 3-O-alpha-L-rhamnopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1-->4)]-beta-D-glucopyranoside; and tuberoside L, 27-O-beta-D-glucopyranosyl-(25R)-5alpha-spirostan-2alpha,3beta,27-triol 3-O-alpha-D-rhamnopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1-->4)]-beta-D-glucopyranoside. New spirostanol saponins from Chinese chives (Allium tuberosum). Sang S1, Zou M, Xia Z, Lao A, Chen Z, Ho CT. No
11601299 Five compounds were isolated from the aerial part of Ampelopsis grossedentata and identified as ampelopsin, ambrein, beta-sitosterol, myricetin and myricitrin by means of UV, IR, 1HNMR and MS spectral analysis. They were isolated from this plant for the first time. [Chemical constituents in aerial part of Ampelopsis grossedentata(Hand.-Mazz.) W.T. Wang]. Yuan A, Huang X, Chen J. No
11601301 Studies on the chemical constituents of Phyllanthus urinaria and its antiviral activity against hepatitis B virus were completed. Eleven compounds have been isolated. Two of them are new compounds methyl ester dehydrochebulic acid and methyl brevifolin carboxylate. Antiviral experiments on HBsAg in vitro and liver damage caused by CCl4 have shown that. Phyllanthus urinaria possesses antiviral activities against HBV. [Chemical constituents of Phyllanthus urinaria L. and its antiviral activity against hepatitis B virus]. Zhong Y, Zuo C, Li F, Ding X, Yao Q, Wu K, Zhang Q, Wang Z, Zhou LW, Lan J, Wang X. No
11601353 OBJECTIVE: To explore the medicinal resources of Crataegus and seek compounds with pharmaceutical effects.METHOD: Column chromatography was employed for the isolation and purification of constituents and the structures were elucidated by spectral analysis and chemical evidence.RESULT: Six compounds were obtained and identified as rutin, hyperoside, vitexin, ursolic acid, daucosterol, and nonacosanol.CONCLUSION: The main constituents of the leaves of Crataegus scabrifolia are flavonoids. [Chemical constituents of the leaves of Crataegus scabrifolia (Franch.) Rehd]. Si J, Gao G, Chen D. No
11705026 The main compounds of lipophilic extracts of flower heads of marigold (Calendula officinalis L.) are triterpendiol esters, mainly faradiol laurate, faradiol myristate and faradiol palmitate. These faradiol-3-O-monoesters have been quantified for the first time by means of reversed phase HPLC with internal standardisation in different parts of C. officinalis plants, namely ray florets, disk florests, involucral bracts, receptacles, levaes and seeds. The amounts of the esters were highest in ray florets, approximately 10 times lower in disk florets than in the ray florets, and approximately 10 times lower in involucral bracts than in the disk florets. In the leaves only traces of the esters could be detected, and in the receptacles no esters could be detected at all. Quantification in the seed was not possible using this method because of interfering fatty compounds. Concerning the faradiol esters, the dried ray and disk florets only should be preferred as primary products for remedies as demanded in the recently published supplement of the Pharmacopoiea Europaea (1999). Breeding work should focus on varieties with a greater number of ray florets in order to improve the quality of herbal medicinal products derived from marigoid. Morphogenetic variability of faradiol monoesters in marigold Calendula officinalis L. Zitterl-Eglseer K, Reznicek G, Jurenitsch J, Novak J, Zitterl W, Franz C No
11724237 New dammarane-type triterpene saponins, notoginsenosides-L, -M, and -N, were isolated from the glycosidic fraction of the dried roots of Panax notoginseng (Burk.) F. H. Chen. Their structures were elucidated on the basis of chemical and physicochemical evidence. Immunological adjuvant activities of the principal notoginsenosides and related dammarane-type triterpene saponins were examined and notoginsenosides-D, -G, -H, and -K were found to increase the serum IgG level in mice sensitized with ovalbumin. Bioactive saponins and glycosides. XIX. Notoginseng (3): immunological adjuvant activity of notoginsenosides and related saponins: structures of notoginsenosides-L, -M, and -N from the roots of Panax notoginseng (Burk.) F. H. Chen. Yoshikawa M1, Morikawa T, Yashiro K, Murakami T, Matsuda H. No
11730876 Bioassay-directed fractionation of the CH(2)Cl(2)-MeOH (1:1) extract of the stem bark of Erythrina indica, has resulted in the isolation of two new isoflavone derivatives named indicanines D and E together with 11 known compounds including: six isoflavones (genistein, wighteone, alpinumisoflavone, dimethylalpinumisoflavone, 8-prenyl erythrinin C, and erysenegalensein E), one cinnamate (erythrinassinate B), two pentacyclic triterpenes (oleanolic acid and erythrodiol), and two phytosterols (stigmasterol and its 3-O-beta-D-glucopyranoside). The structures of the new compounds were elucidated by means of spectroscopic analysis. The in vitro cytocidal activity against KB cells of some of the isolated compounds is also reported. Cytotoxic isoflavones from Erythrina indica. Nkengfack AE1, Azebaze AG, Waffo AK, Fomum ZT, Meyer M, van Heerden FR. No
11731930 A new lignan glycoside, named eleutheroside E(2) (1), has been isolated from the roots of Eleutherococcus senticosus (known as "Siberian ginseng"), along with isomaltol 3-O-alpha-D-glucopyranoside (2), eleutherosides B, E and E(1), and thymidine. The structure of 1 was established by spectral interpretations as episyringaresinol 4"-O-beta-D-glucopyranoside. Compound 2 is described here for the first time as a naturally occurring compound. A new lignan glycoside from Eleutherococcus senticosus. Li XC, Barnes DL, Khan IA. No
11738416 From the stems of Securidaca inappendiculata, securixanthones A (1,3,7-trihydroxy-2,8-dimethoxyxanthone) and B (3,7-dimethoxy-4-hydroxyxanthone) along with ten known xanthones were isolated. Their structures were elucidated by analysis chemical and spectroscopic evidence, and the chemotaxonomic significance of these findings are also discussed. Xanthones from the stems of Securidaca inappendiculata. Yang XD, Xu LZ, Yang SL. No
11745024 Two new steroidal saponins, dioscoresides C (1) and D (2), along with a new natural product, pregnadienolone 3-O-beta-gracillimatriose (3), and two known compounds, pregnadienolone 3-O-beta-chacotrioside (4) and pseudoprotodioscin (5), were isolated from the rhizomes of Dioscorea panthaica Prain et Burkill. On the basis of extensive NMR studies and chemical evidence, dioscoresides C and D were determined to be 26-O-beta-D-glucopyranosyl-3 beta,26-dihydroxy-23(S)-methoxy-25(R)-furosta-5,20(22)-dien-3-O-alpha-L-rhamnopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1-->4)]-beta-D-glucopyranoside and 26-O-beta-D-glucopyranosyl-3 beta,26-dihydroxy-20,22-seco-25(R)-furosta-5-en-20,22-dine-3-O-alpha-L-rhamnopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1--> 4)]-beta-D-glucopyranoside. These compounds showed mild cytotoxicity against the cancer cell lines, A375, L929, and HeLa, in a dose-dependent manner. Two new steroidal saponins from the rhizomes of Dioscorea panthaica and their cytotoxic activity. Dong M1, Feng XZ, Wu LJ, Wang BX, Ikejima T. No
11745034 The essential oils of Shorea robusta heartwood and resin were isolated by hydrodistillation of their respective petroleum ether extracts. Nine and seventeen compounds representing 80.35 % and 78.43 % of the oil, respectively were identified by GC-MS. Germacrene-D was found to be the chief constituent of both the oils. This is the first report on heartwood and resin oils of Shorea robusta. GC-MS analysis of essential oils of heartwood and resin of Shorea robusta. Kaur S, Dayal R, Varshney VK, Bartley JP. No
11794526 Six bioactive alkaloids, aristolactam B(1), piperolactam A(2), aristolactam A(3), norcepharadione B(4), cepharadione B(5) and splendidine(6) were isolated by bioactivity-guided fractionation of a methanolic extract of the aerial part of Houttuynia cordata. Several of them exhibited significant cytotoxicity against five human tumor cell lines (A-549, SK-OV-3, SK-MEL-2, XF-498 and HCT-15) in vitro. Cytotoxic alkaloids from Houttuynia cordata. Kim SK1, Ryu SY, No J, Choi SU, Kim YS.
11808064 Neem is one of the most widely researched tropical tree, with almost all it's parts being put for a variety of uses. In the present study, the antibacterial effect of Neem mouthwash against salivary levels of streptococcus mutans and lactobacillus has been tested over a period of 2 months. Also it's effect in reversing incipient carious lesions was assessed. While streptococcus mutans was inhibited by Neem mouthwashes, with or without alcohol as well as chlorhexidine, lactobacillus growth was inhibited by chlorhexidine alone. The initial data appears to prove it's effect in inhibiting S. mutans and reversing incipient carious lesions, longer term clinical trials are essential. The effect of indigenous Neem Azadirachta indica [correction of (Adirachta indica)] mouth wash on Streptococcus mutans and lactobacilli growth. Vanka A, Tandon S, Rao SR, Udupa N, Ramkumar P. No
11830152 Three new (1-3) and two known (4-5) triterpene glycosides were isolated from the leaves of Acanthopanax japonicus (Araliaceae) and elucidated structurally by mass, 1D, and 2D NMR spectroscopy. All the compounds possessed a nor-oleanene triterpene skeleton as the aglycone. The structures of 1-5 were established as 28-O-alpha-L-rhamno-pyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl ester of 3beta-hydroxy- 30-nor-olean-12,20(29)-diene-23,28-dioic acid, designated as acanjaposide A, 3beta- hydroxy-23-oxo-30-nor-olean-12,20(29)-diene-28-oic acid, named acanjaposide B, 3beta,20alpha-dihydroxy-23-oxo-30-nor-olean-12-en-28-oic acid, named acanjaposide C, and nipponoside E, a known saponin, respectively. nor-Oleanene type triterpene glycosides from the leaves of Acanthopanax japonicus.
No
11842325 Two new xanthones, namely caledonixanthones E (1) and F (2), were isolated from the stem bark of Calophyllum caledonicum (Guttiferae). The structural elucidation of these compounds was mainly established on the basis of 1D, 2D NMR and HRMS spectroscopic analysis. Among the isolated compounds, eight other known xanthones were also identified in the course of this phytochemical study. In addition to this report, a preliminary evaluation of the antifungal properties of these polyphenolic compounds against Aspergillus fumigatus and Candida albicans is presented. New and antifungal xanthones from Calophyllum caledonicum. Morel C, Séraphin D, Teyrouz A, Larcher G, Bouchara JP, Litaudon M, Richomme P, Bruneton J. No
11848223 Four new naphthohydroquinones, rubinaphthins A (1), B (2), C (3), and D (4), together with 11 known compounds were isolated and characterized from the roots of Rubia yunnanensis. The structures of 1-4 were elucidated by spectral analysis and chemical transformation. Constituents of the roots of Rubia yunnanensis. Liou MJ1, Wu PL, Wu TS. No
11908967 Nine new polyprenylated benzoylphloroglucinol derivatives, hyperibones A-I (1-9), were isolated from the aerial parts of the Uzbekistan medicinal plant Hypericum scabrum. Their structures were determined mainly on the basis of spectroscopic evidence (2D NMR and HRMS). Compounds 1, 2, and 4 showed mild in vitro antibacterial activity against methicillin-resistance Staphylococus aureus (MRSA) and methicillin-sensitive Staphylococus aureus (MSSA). Benzoylphloroglucinol derivatives from Hypericum scabrum. Matsuhisa M, Shikishima Y, Takaishi Y, Honda G, Ito M, Takeda Y, Shibata H, Higuti T, Kodzhimatov OK, Ashurmetov O. No
11909635 Phytochemical investigation of the dried leaves of Syringa afghanica, has led to the isolation of nine secoiridoid glucosides, safghanosides A-H and 2"-epi-frameroside, as well as an iridoid glucoside, syringafghanoside along with nineteen known compounds. The structures were elucidated by spectroscopic and chemical means. Secoiridoid and iridoid glucosides from Syringa afghanica. Takenaka Y1, Okazaki N, Tanahashi T, Nagakura N, Nishi T. No
11911204 From the leaves of Alangium paltanifolium var. platanifolium, collected in Fukuoka Prefecture, twelve further megastigmane glycosides were isolated. Seven of them, named platanionosides D-J (1-7), were found to be new compounds. Their structures were elucidated from spectroscopic evidence and their absolute structures were determined from beta-D-glucosylation-induced shift trends of 13C-NMR and by application of a modified Mosher's method. Platanionosides D-J: megastigmane glycosides from the leaves of Alangium platanifolium (Sieb. et Zucc.) Harms var. platanifolium Sieb. et Zucc. Otsuka H1, Tamaki A. No
12016899 Demethyltenuicausine (I), a new bisindole alkaloid, was isolated from Melodinus hemsleyanus and its structure was determined by spectral analysis and semisynthesis. This compound showed antitumor activities in pharmacologicat tests.

PMID:
12016899
[PubMed - indexed for MEDLINE]
[Demethyltenuicausine, a new bisindole alkaloid from Melodinus hemsleyanus]. Yan K, Hong S, Feng X. No
12027738 Five new indole alkaloids of the ibogan type (1-5), in addition to 12 other known iboga alkaloids, were obtained from the leaf and stem-bark extract of the Malayan species Tabernaemontana corymbosa, viz., 19(S)-hydroxyibogamine (1), 19-epi-isovoacristine (2), isovoacryptine (3), 3R/S-ethoxyheyneanine (4), and 3R/S-ethoxy-19-epi-heyneanine (5). The structures were determined using NMR and MS analysis and comparison with known related compounds. Five new iboga alkaloids from Tabernaemontana corymbosa. Kam TS1, Sim KM. No
12027751 The new diterpene glucoside 3,15-dihydroxy-ent-labd-7-en-17-oic acid 3-O-beta-D-glucoside (1) and its aglycone (2) have been isolated from Eupatorium glutinosum. The structures were determined by IR, one- and two-dimensional NMR, high-resolution mass spectrometry, chemical transformations, and comparison of spectroscopic data with closely related diterpenes. Crude extracts showed antimicrobial and cytotoxic activities, but compounds 1 and 2 showed only antimicrobial activity. These results support the vernacular medicinal use of the plant as an antimicrobial. New labdene iterpenes from Eupatorium glutinosum. El-Seedi HR, Sata N, Torssell KB, Nishiyama S. No
12031429 A new prenylated xanthone, named allanxanthone A, was isolated from the stem bark of Allanblackia floribunda in addition to known compounds, 1,5-dihydoxyxanthone, 1,5,6-trihydroxy-3,7-dimethoxyxanthone, stigmasterol and stigmasteryl-3-O-beta-D-glucopyranoside. The structure of the new compound was assigned as 1,3,5-trihydroxy-2-(3-methylbut-2-enyl)-4-(1,1-dimethylprop-2-enyl) xanthone, by means of spectroscopic analysis. The 13C NMR spectral data of 1,5-dihydroxyxanthone is reported here for the first time as well as the in vitro cytotoxic activity of xanthone metabolites against the KB cell line. A prenylated xanthone from Allanblackia floribunda. Nkengfack AE, Azebaze GA, Vardamides JC, Fomum ZT, van Heerden FR. No
12036019 From leaves of Turpenia ternata (Staphylaceae), one megastigmane and seven of its glucosides (1-8) were isolated. Megastigmane and two of the glucosides were found to be known compounds, namely, 3S,5R,6R,9S-tetrahydroxymegastigmane (1), corchoionoside C (2), and icariside B4 (3). The structures of compounds 4-8 (turpinionosides A-E, respectively) were elucidated by means of spectroscopic analyses, and then their absolute structures were determined by the modified Mosher's method to be (3S,5R,6S,9S)-3,6,9-trihydroxymegastigman-7-ene 3-O- and 9-O-beta-D-glucopyranosides (4, 5, respectively), (1S,3S,5R,6S,9R)-3,9,12-trihydroxymegastigmane 3-O-beta-D-glucopyranoside (6), (3S,4R,9R)-3,4,6-trihydroxymegastigman-5-ene 3-O-beta-D-glucopyranoside (7), and (2S,9R)-2,9-dihydroxymegastigman-5-en-4-one 2-O-beta-D-glucopyranoside (8). Turpinionosides A-E: megastigmane glucosides from leaves of Turpinia ternata Nakai. Yu Q1, Otsuka H, Hirata E, Shinzato T, Takeda Y. No
12049217 Three pentacyclic triterpenoids including one new guajavanoic acid (2) and two known obtusinin (1) and goreishic acid I (3) have been isolated from the leaves of Psidium guajava. The new constituent 2 has been characterized as 2alpha-hydroxy-3beta-p-E-coumaroyloxyurs-12, 18-dien-28-oic acid through 1H-NMR and 13C-NMR (broad band and DEPT). This is the first report of isolation of compound 1 and 3 from the genus Psidium. Triterpenoids from Psidium guajava leaves. Begum S, Siddiqui BS, Hassan SI. No
12067160 Three new xanthone glycosides, securixanside A (1), securixanside B (2), and securixanside C (3) were isolated from the stems of Securidaca inappendiculata. These compounds were characterized by spectrometric and chemical methods, including FABMS and one- and two-dimensional NMR experiments. New xanthone glycosides from Securidaca inappendiculata. Yang XD, An N, Xu LZ, Yang SL. No
12135099 The ethyl acetate extract of the leaves of Ixeris sonchifolia afforded two new and two known sesquiterpene lactone glucosides of the guaiane-type, together with a known alkenol glucoside. The known compounds were identified as ixerin Z (1), ixerin Z-6'-p-hydroxyphenylacetate (2), and (Z)-3-hexen-1-ol-beta-D-glucopyranoside (3), respectively. The structures of the new compounds were elucidated as 11,13a-dihydroixerin Z [4, 3-hydroxy-2-oxo-guaia-1(10), 3-dien-5alpha,6beta,7alpha, 11betaH-12,6-olide-3-O-beta-D-glucopyranoside], and 3,10beta-dihydroxy-2-oxo-guaia-3,11(13)-dien-1alpha,5alpha,6alpha,7aH-12,6-olide-10-O-beta-D-glucopyranoside (5), respectively. The cytotoxicity of these compounds against human hepatocellular carcinoma cell (HepG2) and human melanoma cell (SK-MEL-2) was evaluated. Cytotoxic constituents of the leaves of Ixeris sonchifolia. Suh J, Jo Y, Kim ND, Bae SJ, Jung JH, Im KS. No
12141854 Bioassay-guided fractionation of a CH2Cl2-MeOH extract of the bark of Ochrocarpos punctatus resulted in the isolation of seven new coumarins, ochrocarpins A-G (1-7), three new benzophenone derivatives, ochrocarpinones A-C (8-10), and five known coumarins, mammea A/AC cyclo F (11), mammea A/AD cyclo D (12), mammea A/AB cyclo F (13), mammea A/AA cyclo F (14), mammea A/AB cyclo D (15), and 15,16-dihydro-16-hydroperoxyplukenetione (16). The structures of compounds 1-10 were established on the basis of extensive 1D and 2D NMR spectroscopic data interpretation. All compounds exhibited cytotoxicity against the A2780 ovarian cancer cell line. New cytotoxic coumarins and prenylated benzophenone derivatives from the bark of Ochrocarpos punctatus from the Madagascar rainforest. Chaturvedula VS, Schilling JK, Kingston DG. No
12141879 A new norditerpene alkaloid was isolated, 18-demethylpubescenine (1), in addition to four known compounds, 14-demethyltuguaconitine (2), takaosamine (3), gigactonine (4), and delcosine (5), from fresh, whole plants of Consolida orientalis. The structure of 1 was established by spectroscopic methods, including various 2D NMR techniques and HRESIMS. As a result of a detailed NMR study, complete 1H NMR chemical shift assignments for alkaloids 1-5 are presented herein, and some 13C NMR signal assignments for 2-4 have been revised. Norditerpenoid alkaloids from Consolida orientalis and complete 1H and 13C NMR signal assignments of some lycoctonine-type alkaloids. Hohmann J, Forgo P, Hajdú Z, Varga E, Máthé I. No
12150799 Three prenylated flavonoid derivatives; 5,7,4'-trihydroxy-8-(3"'-methylbut-2"'-enyl)-6-(2"-hydroxy-3"-methylbut-3"enyl) isoflavone (isoerysenegalensein E), 5,7,2'-trihydroxy-4'-methoxy-5'-(3"-methylbut-2"-enyl) isoflavanone (lysisteisoflavanone), 5, 4'-dihydroxy-6-(3"'-methylbut-2"'-enyl)-2"-hydroxyisopropyl dihydrofurano [4",5":8,7] isoflavone (isosenegalensin), together with the four known flavonoids abyssinone V-4'-methylether, alpinumisoflavone, wighteone and burttinone were isolated from the stem bark of Erythrina lysistemon Hutch. (Leguminosae). Structures were elucidated by spectroscopic methods. Prenylated flavonoids of Erythrina lysistemon grown in Egypt. el-Masry S1, Amer ME, Abdel-Kader MS, Zaatout HH. No
12188619 Cascarilla is a commercially available and cheap source of polyfunctionalized diterpenoids belonging to the clerodane structural type. In addition to the bitter triol cascarillin, 10 additional new diterpenoids (eluterins A-J) have been isolated and characterized by spectroscopic means. Structural diversity within cascarilla clerodanes involves mainly the linkage between the carbocyclic and the heterocyclic moieties and the functionalization of C-3, C-4, and C-6 of the decalin core. Cascarillin was shown to be a mixture of interconverting gamma-lactols and not a gamma-hydroxyaldehyde as previously reported. Diterpenoids from Cascarilla (Croton eluteria Bennet). Fattorusso E1, Taglialatela-Scafati O, Campagnuolo C, Santelia FU, Appendino G, Spagliardi P. No
12240986 Tanacetum santolinoides, Essential Oil Composition, n-Hexane-Ether Extract Composition The essential oil of the aerial parts of Tanacetum santolinoides was analyzed by capillary GLC and GLC-MS. Altogether 30 components were identified. The main constituents were thymol (18%), trans-thujone (17.5%), trans-chrysanthenyl acetate (13.2%), cis-chrysanthenyl acetate (9.2%), umbellulone (9.7%) and 1,8-cineole (4.7%). Similar essential oil pattern in addition to palmitic acid methyl ester, palmitic acid, stigmasterol, sitosterol and two flavonoidal aglycons were found in the n-hexane-ether extract. The oil showed strong in vitro activity against E. coli, Bacillus subtilis and Candida albicans. Composition and antimicrobial activity of essential oil and hexane-ether extract of Tanacetum santolinoides (dc.) Feinbr. and Fertig. El-Shazly A, Dorai G, Wink M. No
12242822 OBJECTIVE: To investigate the chemical constituents of Aralia subcapitata.METHOD: The methanol extract of A. subcapitata was separated by repeated column chromatography, and the compounds were identified on the basis of spectroscopic analysis(IR, EI-MS, 1HNMR and 13CNMR) and physicochemical properties.RESULT: Four compounds were isolated from the root bark of A. subcapitata. Their structures were identified as 3 beta-hydroxy-oleana-11, 13(18)-dien-28-oic acid(I), 3-oxo-oleana-12-en-28-oic acid (II), oleanolic acid(III) and daucosterol(IV).CONCLUSION: All these compounds were isolated from this plant for the first time. Among them, compounds I and II were obtained for the first time from Aralia. [Triterpenoids from the root bark of Aralia subcapitata]. Xu X, Zhang Y, Yang J, Zhu Z. No
12350145 Four new alkylene resorcinols, (Z,Z)-5-(trideca-4,7-dienyl)resorcinol (1), (Z,Z,Z)-5-(trideca-4,7,10-trienyl)resorcinol (2), (Z,Z,E)-5-(trideca-4,7,10-trienyl)resorcinol (3), and (Z)-5-(trideca-4-enyl)resorcinol (4), were isolated from the MeOH-CH(2)Cl(2) extract of Lithraea molleoides. The structures of these compounds were determined by one- and two-dimensional NMR including selective decoupling experiments. In vitro all four compounds showed strong paralyzing effects on the nematode Caenorhabditis elegans at concentrations between 6 and 50 microg/mL, whereas the activity of compounds 1 and 2 against the nematode Trichostrongylus colubriformis was less pronounced and no activity against this nematode was observed for compounds 1-4 in a rodent model. Nematicidal alkylene resorcinols from Lithraea molleoides. Valcic S, Wächter GA, Eppler CM, Timmermann BN. No
12350148 Five new triterpenoids, rubiarbonones D (1), E (5), and F (2), and rubiarbosides F (3) and G (4), together with nine known compounds, were isolated from the roots of Rubia yunnanensis. The structures of 1-12 were elucidated by spectroscopic methods. The antiplatelet aggregation activities of rubiarbonone A (6) and rubiarbonol A (8) and B (9) were investigated with a standard protocol. Triterpenoids from Rubia yunnanensis. Liou MJ1, Wu TS. No
12365606 Six flavonoids have been isolated from the leaves of lemon balm (Melissa officinalis L., Lamiaceae). Their structures were determined on the basis of spectral data (UV, 1R, 1H NMR, 13C NMR and FAB MS) as luteolin, luteolin 7-O-beta-D-glucopyranoside, apigenin 7-O-beta-D-glucopyranoside, luteolin 7-O-beta-D-glucuronopyranoside, luteolin 3'-O-beta-D-glucuronopyranoside and luteolin 7-O-beta-D-glucopyranoside-3'-Obeta-D-glucuronopyranoside. The last three glycosides have been found in lemon balm for the first time and luteolin 7-O-beta-D-glucopyranoside-3'-O-beta-D-glucuronopyranoside is a new compound found in plants. Flavonoids from lemon balm (Melissa officinalis L., Lamiaceae). Patora J, Klimek B.
12377233 Two triterpenoids, 20beta-acetoxy-2alpha,3beta-dihydroxyurs-12-en-28-oic acid (guavanoic acid, 3), and 2alpha,3beta-dihydroxy-24-p-z-coumaroyloxyurs-12-en-28-oic acid (guavacoumaric acid, 7), along with six known compounds 2alpha-hydroxyursolic acid (1), jacoumaric acid (2), isoneriucoumaric acid (4), asiatic acid (5), ilelatifol D (6) and beta-sitosterol-3-O-beta-D-glucopyranoside (8), have been isolated from the leaves of Psidium guajava. Their structures were determined through spectroscopic methods. Compound 5 showed dose-dependent (10-500 microg/ml) spasmolytic activity in spontaneously contracting isolated rabbit jejunum preparations. Triterpenoids from the leaves of Psidium guajava. Begum S, Hassan SI, Siddiqui BS, Shaheen F, Ghayur MN, Gilani AH. No
12406606 The authors report on the HPLC investigation of the carotenoid composition of the steams, leaves, petals and pollens of Calendula officinalis L. In the petals and pollens, the main carotenoids were flavoxanthin and auroxanthin while the stem and leaves mostly contained lutein and beta-carotene. Five different herbal tea and two tinctures made from the flower of C. officinalis L. were also investigated and the carotenoid composition of the industrial products was compared to the starting material. HPLC study on the carotenoid composition of Calendula products. Bakó E, Deli J, Tóth G No
12444688 Bioassay-directed fractionation of a CH(2)Cl(2)-MeOH extract of the leaves of Oncostemon bojerianum resulted in the isolation of eight new 5-alkylresorcinols, named oncostemonols A-H (1-8), and two known derivatives, (8'Z)-1,3-dihydroxy-5-[16'-(3' ',5' '-dihydroxyphenyl)-8'-hexadecen-1'-yl]benzene (9) and (8'Z)-1,3-dihydroxy-5-[14'-(3' ',5' '-dihydroxyphenyl)-8'-tetradecen-1'-yl]benzene (10). The structures of the new compounds 1-8 were elucidated on the basis of extensive 1D and 2D NMR spectroscopic interpretation and chemical derivatization. All the compounds exhibited cytotoxic activity against the A2780 ovarian cancer cell line. New cytotoxic bis 5-alkylresorcinol derivatives from the leaves of Oncostemon bojerianum from the Madagascar rainforest. Chaturvedula VS, Schilling JK, Miller JS, Andriantsiferana R, Rasamison VE, Kingston DG. No
12453526 From the chloroform extract of the root of Ecbolium linneanum Kurz., a furofuran type of unsymmetrical lignan named as Ecbolin A was isolated. The structure was established by spectroscopic methods and confirmed by single crystal X-ray diffraction studies. A lignan from the root of Ecbolium linneanum Kurz. Venkataraman R, Gopalakrishnan S. No
12494342 Bioactivity-guided fractionation of petroleum ether- and EtOAc-soluble extracts of the seeds of Ziziphus jujuba using a cyclooxygenase-2 assay as a monitor indicated that the triglyceride, 1,3-di-O-[9(Z)-octadecenoyl]-2-O-[9(Z),12(Z)-octadecadienoyl]glycerol (3), and a fatty acid mixture of linoleic, oleic and stearic acids, were the major active components. A new pentacyclic lupane-type triterpene derivative, 3-O-[9(Z)-octadecenoyl]betulinic acid (1), and betulinic acid (2) were also isolated and identified. All isolates as well as pure linoleic, oleic and stearic acids were evaluated for their inhibitory effects against both cyclooxygenases-1 (COX-1) and -2 (COX-2). Activity-guided fractionation of the seeds of Ziziphus jujuba using a cyclooxygenase-2 inhibitory assay Su BN, Cuendet M, Farnsworth NR, Fong HH, Pezzuto JM, Kinghorn AD. No
12494352 Two new triterpenoids, guajavolide (2 alpha,3 beta,6 beta,23-tetrahydroxyurs-12-en-28,20 beta-olide; 1) and guavenoic acid (2 alpha,3 beta,6 beta,23-tetrahydroxyurs-12,20(30)-dien-28-oic acid; 2) along with one known triterpene oleanolic acid (3) were isolated from the fresh leaves of Psidium guajava. Their structure elucidation and stereochemistry were determined by spectroscopic experiments, including 2D-NMR techniques. Two new triterpenoids from the fresh leaves of Psidium guajava. Begum S, Hassan SI, Siddiqui BS. No
12515224 OBJECTIVE: To study the chemical constituents of Lagopsis supina.METHOD: Compounds were separated by solvent extraction, column chromatography and TLC, and the structures were determined by chemical and spectral analyses.RESULTS: Six compounds were isolated from the ethanol extract of L. supina and identified as eicosanoic acid octadecyl ester, eicosanoic acid-16-methyl-15, 16-hetadecenyl ester, palmitic acid, beta-sitosterol, oleanolic acid and daucosterol.CONCLUSION: Except beta-sitosterol, all the compounds were isolated from this genus for the first time. [Studies on chemical constituents of Lagopsis supina (Steph.) Ik.-GaI]. Yuan JR, Li QW, Li ZL. No
12521264 This review covers the structures of compounds with antiplasmodial activity isolated from plants and is organized according to plant family. A total of 170 structures has been reviewed from 186 references found in the literature up to December 2000. Antimalarial activity of plant metabolites. Schwikkard S, van Heerden FR. No
12525072 OBJECTIVE: To isolate and elucidate the chemical constituents in the roots of Rabdosia japonica.METHOD: The constituents were extracted by ether and isolated by chromatography on silica gel and ODS. The structures were determined by IR, H NMR, 13C NMR and MS spectra respectively.RESULT: Four compounds were elucidated as glaucocalgxin A, oleanolic acid, ursolic acid and daucosterol.CONCLUSION: Oleanolic acid and daucosterol were isolated from this plant for the first time. [Studies on chemical constituents in the roots of Rabdosia japonica (Burm.f). Hara var. glaucocalyx (Maxin) Hara]. Jin YR, Gui MY, Wang BZ. No
12525120 OBJECTIVE: To study the chemical constituents in the dried buds of Lonicera similis.METHOD: Chromatography and spectral analysis were used to isolate and elucidate the constituents.RESULT: Nine compounds were isolated from the dried buds of L. similis, and identified as caffeic acid; 3,5-O-dicaffeoylquinic acid; chlorogenic acid; palmitic acid; luteolin; quercetin; beta-sitosterol; nonacosanol; nonacosane.CONCLUSION: All the compounds were isolated from the plant for the first time. [Studies on chemical constituents in dried buds of Lonicera similis Hemsl]. Li YM, Wang TZ, Wang ZX. No
12542336 Ten new bisindole alkaloids of the vobasinyl-ibogan type, viz., conodiparines A-F (1-6), conodutarines A and B (7, 8), and cononitarines A and B (9, 10), were obtained from the leaf extract of the Malayan species Tabernaemontana corymbosa. The structures were determined using NMR and MS analysis. New bisindole alkaloids from Tabernaemontana corymbosa. Kam TS1, Sim KM, Pang HS. No
12542368 Four new cephalotaxus alkaloids, cephalotaxine alpha-N-oxide (1), cephalotaxine beta-N-oxide (2), 11-beta-hydroxycephalotaxine beta-N-oxide (3), and isocephalotaxine (4), were isolated, together with several known alkaloids from an EtOAc extract of the fruits of Cephalotaxus fortunei. The structures were determined by spectral analysis including mass spectrometry and 2D NMR. Compounds 1, 2, 3, and 4 displayed cytotoxicity against nasopharynx KB cells with IC50 values of 30, 14, 31, and 15 micro g/mL, respectively. New alkaloids from Cephalotaxus fortunei. Bocar M, Jossang A, Bodo B. No
12560028 Five oleanane-type pentacyclic triterpenoids were isolated by chromatographic separation of a chloroform extract of the stem bark of Embelia schimperi. Three of these compounds have a methyleneoxy bridge. Two compounds, embelinone and schimperinone, are reported here for the first time from a natural source (they have been synthesized previously during chemical transformations). Their structures were determined by spectroscopic techniques, among which 2-D NMR was useful for complete characterization. Three of the triterpenoids exhibited mild antibacterial properties against the gram-positive bacterial strain Rhodococcus sp. Pentacyclic triterpenoids from Embelia schimperi. Machocho AK, Kiprono PC, Grinberg S, Bittner S. No
12567778 AIM: To study the active constituents of Swertia davidi Franch..METHODS: Chromatography was used to isolate and purify the chemical components, their structures were identified by spectral analysis.RESULTS: Three compounds were identified as 1,7-dihydroxy-3,8-dimethoxyxanthone (gentiacaulein) (V), 1,8-dihydroxy-3,7-dimethoxyxanthone (methylswertianin) (VI) and 1,8-dihydroxy-3,4,7-trimethoxyxanthone (VII).CONCLUSION: Compound VII is a novel xanthone, named daviditin A, the others were isolated from Swertia davidi Franch. for the first time. [Studies of the chemical constituents of Swertia davida Franch]. Tan GS, Xu KP, Xu PS, Hu GY, Li YJ. No
12583176 OBJECTIVE: To study the chemical constituents from Ampelopsis grossedentata (hand-Mazz) W.T. Wang.METHODS: Chromatography and spectroscopic analysis were employed to isolate and elucidate the chemical constituents in the plant.RESULTS: Seven compounds were isolated and elucidated as ampelopsin (I), myricetin (II), myricitrin (III), gallic acid (IV), beta-sitosterol (V), stigmasterol (VI) and dihydroquercetin (VII).CONCLUSION: Among these compounds, IV, V, VI and VII were isolated from this plant for the first time, and compound VII was obtained from genus Ampelopsis for the first time. [Studies on the chemical constituents from Ampelopsis grossedentata]. Wang Y, Zhou L, Li R, Wang Y. No
12608849 We describe the isolation and spectrometric structure elucidation of eight new xanthones, fuscaxanthone A (1), B (2), C (3), D (4), E (5), F (6), G (7), and H (8), together with eight known xanthones from the stem bark of Garcinia fusca collected in Thailand. All the new xanthones were shown to have a terpenoid (prenyl and/or geranyl) side chain(s) in their molecules. We also present the results of a primary screening of the inhibitory effects of eight xanthones (9-16) isolated as major components of this plant on 12-O-tetradecanoylphorbol-13-acetate induced Epstein-Barr virus early antigen activation in Raji cells. Chemical constituents of Garcinia fusca: structure elucidation of eight new xanthones and their cancer chemopreventive activity. Ito C, Itoigawa M, Takakura T, Ruangrungsi N, Enjo F, Tokuda H, Nishino H, Furukawa H. No
12608871 Three new 2'-oxygenated flavonoids, (2S)-5,7,2',3',4'-pentamethoxyflavanone (1), 5-hydroxy-7,8,2',5'-tetramethoxyflavone (2), and echioidinin 2'-O-beta-d-(6' '-O-acetyl) glucopyranoside (3), together with four known flavonoids, 7-O-methyldihydrowogonin, 7-O-methylwogonin, skullcapflavone I 2'-methyl ether, and skullcapflavone I, and two diterpenoids, andrograpanin and 14-deoxy-11,12-didehydroandrographolide, were isolated from the whole plant of Andrographis affinis. The structures were elucidated by spectral and chemical studies. New 2'-oxygenated flavonoids from Andrographis affinis. Bhaskar Reddy MV, Kishore PH, Rao CV, Gunasekar D, Caux C, Bodo B. No
12608878 Two new 27-nor-triterpene glycosides, tomentosides A (1) and B (2), were isolated from Peruvian "Uña de Gato" (cat's claw, plant of origin: Uncaria tomentosa), a traditional herbal medicine in Peru. Their structures were determined by spectroscopic analysis and chemical interconversions. This is the first report of naturally occurring pyroquinovic acid glycosides. Two new nor-triterpene glycosides from peruvian "Uña de Gato" (Uncaria tomentosa). Kitajima M, Hashimoto K, Yokoya M, Takayama H, Sandoval M, Aimi N. No
12611281 From the aerial parts of Consolida scleroclada (Boiss.) Schrod. collected in Turkey, a new diterpenoid alkaloid named willipelletierine has been isolated along with the known diterpenoid alkaloids aconitine, lapaconidine, and delcosine. The structure for willipelletierine was established on the basis 1H, 13C, DEPT, homonuclear 1H COSY, HETCOR and NOESY NMR studies. Willipelletierine, a new diterpenoid alkaloid from Consolida scleroclada (Boiss.) Schrod. Meriçli F, Meriçli AH, Seyhan GV, Bahar M, Desai HK, Ozçelik H, Ulubelen A. No
12612412 Five megastigmane glucosides were isolated from the leaves of Glochidion zeylanicum. One of them was a known compound, blumenol C O-beta-D-glucopyranoside (1), and the structures of the four new compounds, glochidionionosides A-D (2-5), were mainly elucidated by spectroscopic methods, including a modified Mosher's method. The absolute configurations of the six-membered ring of glochidionionoside D (5) were deduced by beta-D-glucopyranosylation-induced shift trends in the (13)C-NMR spectra and confirmed by X-ray analysis as its p-bromobenzoate (5b), and the axis chirality of C-7 was determined to be R. Glochidionionosides A-D: megastigmane glucosides from leaves of Glochidion zeylanicum (Gaertn.) A. Juss. Otsuka H1, Kijima H, Hirata E, Shinzato T, Takushi A, Bando M, Takeda Y. No
12628419 Ganodermadiol, lucidadiol and applanoxidic acid G were isolated as first triterpenes from the European Basidiomycete Ganoderma pfeifferi. The compounds show antiviral activity against influenza virus type A and HSV type 1. Antiviral lanostanoid triterpenes from the fungus Ganoderma pfeifferi. Mothana RA, Awadh Ali NA, Jansen R, Wegner U, Mentel R, Lindequist U. No
12648543 A prenylated flavonoid, moralbanone, along with seven known compounds kuwanon S, mulberroside C, cyclomorusin, eudraflavone B hydroperoxide, oxydihydromorusin, leachianone G and alpha-acetyl-amyrin were isolated from the root bark of Morus alba L. Leachianone G showed potent antiviral activity (IC(50) = 1.6 microg/ml), whereas mulberroside C showed weak activity (IC(50) = 75.4 microg/ml) against herpes simplex type 1 virus (HSV-1). Their structures were elucidated by spectroscopic methods. Antiviral flavonoids from the root bark of Morus alba L. Du J, He ZD, Jiang RW, Ye WC, Xu HX, But PP. No
12648545 Two 3-phenoxychromones, eryvarins F and G, were isolated from the roots of Erythrina variegata. Their structures were established to be 3-(2,4-dihydroxyphenoxy)-7-hydroxy-6,8-di(3,3-dimethylallyl)chromen-4-one and 3-(2,4-dihydroxyphenoxy)-8-(3,3-dimethylallyl)-2,2-dimethylpyrano[5,6:6,7]chromen-4-one on the basis of spectroscopic and chemical evidence. Eryvarins F and G are unusual 3-phenoxychromone derivatives with two isoprenoid groups. Eryvarins F and G, two 3-phenoxychromones from the roots of Erythrina variegata. Tanaka H1, Hirata M, Etoh H, Shimizu H, Sako M, Murata J, Murata H, Darnaedi D, Fukai T. No
12648813 Kuwanon G was isolated from the ethyl acetate fraction of methanol extract of Morus alba and its structure was elucidated by 13C-NMR, 1H-NMR and FAB-MS. Antibacterial activity of kuwanon G was investigated by the minimum inhibitory concentration (MIC) test and the viable cell count method. MIC of kuwanon G against Streptococcus mutans causing dental caries was determined to be 8.0 microg/ml. The bactericidal test showed that kuwanon G completely inactivated S. mutans at the concentration 20 microg/ml in 1 min. Kuwanon G also significantly inhibited the growth of other cariogenic bacteria such as Streptococcus sobrinus and Streptococcus sanguis, and Porpyromonas gingivalis causing periodontitis. Transmission electron microscopy (TEM) of kuwanon G treated cells demonstrated remarkable morphological damage of the cell wall and condensation of the cytoplasm Kuwanon G: an antibacterial agent from the root bark of Morus alba against oral pathogens. Park KM, You JS, Lee HY, Baek NI, Hwang JK. No
12662111 Two new phenolic compounds, polygalolide A (1) and polygalolide B (2), together with three known xanthones were isolated from the roots and stems of Polygala fallax. The structures of 1 and 2 were elucidated on the basis of spectroscopic evidence. New phenolics from Polygala fallax. Ma W, Wei X, Ling T, Xie H, Zhou W. No
12684889 In the course of the search for new antimalarial compounds, a study of plants traditionally used against malaria in Burkina Faso was made. An ethnobotanical study permitted the identification of plants currently used by the traditional healers and herbalists. Two plants among them were selected for further study: Pavetta crassipes (K. Schum) and Acanthospermum hispidum (DC). Alkaloid extracts of these plants were tested in vitro against two reference clones of Plasmodium falciparum: the W2 chloroquine-resistant and the D6 chloroquine-sensitive strains. Significant inhibitory activity was observed with Pavetta crassipes (IC(50)=1.23 microg/ml) and A. hispidum (IC(50)=5.02 microg/ml). Antiplasmodial activity was also evaluated against six Plasmodium falciparum isolates from children between 4 and 10 years old. The IC(50) values for the alkaloid extracts were in the range 25-670 ng/ml. These results indicated that P. falciparum wild strains were more sensitive to the alkaloid extracts than strains maintained in continuous culture. Moreover, the alkaloid extracts exhibit good in vitro antimalarial activity and weak cytotoxicity against three human cell lines (THP1, normal melanocytes, HTB-66). Isolation and structural determination are now necessary in order to precisely determine the active compounds. Antiplasmodial activity of alkaloid extracts from Pavetta crassipes (K. Schum) and Acanthospermum hispidum (DC), two plants used in traditional medicine in Burkina Faso. Sanon S, Azas N, Gasquet M, Ollivier E, Mahiou V, Barro N, Cuzin-Ouattara N, Traore AS, Esposito F, Balansard G, Timon-David P. No
12713413 Assay-guided fractionation of the ethanol extract of Nymphaea odorata resulted in the identification of two lignans, one new (1) and one known (2), together with six known flavonol glycosides (3-8). The structures of 1-8 were established by spectroscopic analysis as nymphaeoside A (1), icariside E(4) (2), kaempferol 3-O-alpha-l-rhamnopyranoside (afzelin, 3), quercetin 3-O-alpha-l-rhamnopyranoside (4), myricetin 3-O-alpha-l-rhamnopyranoside (myricitrin, 5), quercetin 3-O-(6' '-O-acetyl)-beta-d-galactopyranoside (6), myricetin 3-O-beta-d-galactopyranoside (7), and myricetin 3-O-(6' '-O-acetyl)-beta-d-galactopyranoside (8). Compounds 3, 4, and 7 showed marginal inhibitory effect against fatty acid synthase with IC(50) values of 45, 50, and 25 microg/mL, respectively. Phenolic compounds from Nymphaea odorata. Zhang Z, ElSohly HN, Li XC, Khan SI, Broedel SE Jr, Raulli RE, Cihlar RL, Burandt C, Walker LA. No
12727498 Dichloromethane, methanol and aqueous extracts of the aerial parts of Terminalia australis were evaluated for their antifungal activity. The methanol and aqueous extracts were found to be effective against the tested Aspergillus and Candida strains. Antifungal activity of Terminalia australis. Carpano SM, Spegazzini ED, Rossi JS, Castro MT, Debenedetti SL. No
12762798 The aqueous acetone extract from the roots of a Chinese herbal medicine, Rubia yunnanensis, showed a potent inhibitory effect on nitric oxide production in lipopolysaccharide-activated macrophages. Five new arborinane-type triterpenes, rubianols-a (1), -b (2), -c (3), -d (4), and -e (5), and a new arborinane-type triterpene glycoside, rubianoside I (6), were isolated from the herbal crude extract together with 10 known compounds. The absolute stereostructures of 1-6 were determined on the basis of chemical and physicochemical evidence, including the application of the modified Mosher's method. The effects of the isolated constituents on nitric oxide production in lipopolysaccharide-activated macrophages were examined, and several triterpenes were found to show inhibitory activity. Absolute stereostructures of new arborinane-type triterpenoids and inhibitors of nitric oxide production from Rubia yunnanensis. Morikawa T1, Tao J, Ando S, Matsuda H, Yoshikawa M. No
12762799 A water extract of the rhizomes of Dioscorea spongiosa, which showed antiosteoporotic activity, was examined, and four new pregnane glycosides, named spongipregnolosides A-D (1-4), and two new cholestane glycosides, named spongiosides A (5) and B (6), were isolated together with 15 known glycosides. Their structures were determined on the basis of spectroscopic analysis and chemical methods. Among the isolated compounds, spongioside A (5), hypoglaucin G (7), methylprotodioscin (8), and (R)-oct-1-en-3-yl O-alpha-l-arabinopyranosyl-(1-->6)-alpha-d-glucopyranoside (9) showed potent inhibition against bone resorption induced by parathyroid hormone in a bone organ culture system. Steroidal glycosides from the rhizomes of Dioscorea spongiosa. Yin J1, Kouda K, Tezuka Y, Tran QL, Miyahara T, Chen Y, Kadota S. No
12765199 The new flavone diglycoside 7-O-[6-O-(alpha-L-arabifuranosyl)-beta-D-glucopyranosyl]-gossypetin, named as pyrropetioside, along with 13 known compounds (including 3 artifacts) have been isolated from Pyrrosia petiolosa. Their structures have been elucidated by means of chemical and spectroscopic methods including IR, MS, 1D and 2D NMR techniques Chemical constituents of Pyrrosia petiolosa. Yang C, Shi JG, Mo SY, Yang YC. No
12770595 From the stem bark of Erythrina burttii, a new isoflavone, 5,2',4'-trihydroxy-7-methoxy-6-(3-methylbut-2-enyl)isoflavone (trivial name, 7-O-methylluteone) and a new flavanone, 5,7-dihydroxy-4'-methoxy-3'-(3-methylbutadienyl)-5'-(3-methylbut-2-enyl)flavanone (trivial name, burttinonedehydrate) along with three known isoflavonoids (8-prenylluteone, 3-O-methylcalopocarpin and genistein) were isolated. The structures were determined on the basis of spectroscopic evidence. Two prenylated flavonoids from the stem bark of Erythrina burttii. Yenesew A1, Irungu B, Derese S, Midiwo JO, Heydenreich M, Peter MG. No
12770600 The bark of Garcinia merguensis yielded 10 xanthones, merguenone, 1,5-dihydroxy-6'-methyl-6'-(4-methyl-3-pentenyl)-pyrano(2',3':3,2)-xanthone, subelliptenone H, 8-deoxygartanin, rheediaxanthone A, morusignin G, 6-deoxyjacareubin, 1,3,5-trihydroxy-4,8-di(3-methylbut-2-enyl)-xanthone, rheediachromenoxanthone and 6-deoxyisojacareubin. The structure of merguenone was determined using spectroscopic techniques, mainly 1D and 2D NMR spectroscopy. Xanthones from the bark of Garcinia merguensis. Nguyen LH, Vo HT, Pham HD, Connolly JD, Harrison LJ. No
12770606 An investigation of methanolic extract of Warburgia stuhlmannii leaves has led to the isolation of two new drimane-type sesquiterpene glycosides characterized as mukaadial 6-O-beta-D-glucopyranoside, mukaadial 6-O-alpha-L-rhamnopyranoside together with two other novel flavonol glycosides identified as 3',5'-O-dimethylmyricetin 3-O-beta-D-2",3"-diacetylglucopyranoside and 3'-O-methylquercetin 3-O-beta-D-2",3",4"-triacetylglucopyranoside. The known compounds; mukaadial, deacetylugandensolide, quercetin, kaempferol, kaempferol 3-O-alpha-L-rhamnopyranoside, quercetin 3-O-beta-D-glucopyranoside, kaempferol 7-O-beta-D-glucopyranoside, myricetin 3-O-alpha-L-rhamnopyranoside, quercetin 3-O-alpha-L-rhamnopyranoside, quercetin 3-O-sophoroside and isorhamnetin 3-O-beta-D-glucopyranoside were also isolated from the same extract Flavonol and drimane-type sesquiterpene glycosides of Warburgia stuhlmannii leaves. Manguro LO1, Ugi I, Hermann R, Lemmen P. No
12774356 OBJECTIVE: To investigate the chemical components of the leaves of Cyclocarya paliurus.METHOD: The chemical components were isolated by solvent extraction and column chromatography. The chemical structures were identified on the basis of physic-chemical constant and spectral data.RESULT: Six compounds were isolated and identified as kaempferol(I), quercetin(II), isoquercitrin(III), ellagic acid(IV), daucosterol(V) and cyclocaric acid B(VI).CONCLUSION: Compounds I, II, III and IV were obtained for the first time from this plant. [Studies on the chemical constituents in the leaves of Cyclocarya paliurus]. Yi X, Shi JG, Zhou GX, Xie MY. No
12774374 OBJECTIVE: To investigate the chemical constituents from the root and rhizome of Rheum rhizastachyum.METHOD: The compounds were isolated by column chromatography on silica gel, Sephadex LH-20, MCI-gel CHP-20P separately and their structures were elucidated by chemical and spectral technology.RESULT: Six compounds were isolated and identified as chrysophanol, emodin, gallic acid, sucrose, 1-O-beta-D-glucopyranosyl-chrysophanols, 1-O-beta-D-glucopyranosyl-emodin.CONCLUSION: All the compounds were obtained from this plant for the first time. [Studies on the chemical constituents in root of Rheum rhizastachyum]. Zhao J, Chang JM, Du NS. No
12776319 OBJECTIVE:To isolate and characterize compounds from the stems of Schisandra propinqua.METHOD:Extracting with solvent, isolating by column chromatography and identifying by the spectroscopic methods.RESULT:Six dibenzocyclooctadiene lignans were isolated and identified as tigloylgomisin P(1), angeloylgomisin O(2), angeloylisogomisin O(3), kadsulignan L(4), (+/-) 5,8-epoxyl-6, 7-dimethyl-2',3',2",3"-dimethylenedioxy-4', 1"-dimethyl-1,2:3,4-dibenzo-1, 3-cyclooctadiene(5) and wuweizisu C(6).CONCLUSION:Compounds 4 and 5 were the first two dibenzocyclooctadiene lignans with an 6,9-epoxy bridge cycle discovered in the genus Schisandra. The others were originally isolated from S. propinqua. [Studies on chemical constituents of Schisandra propinqua (Wall.) Hook. f. et Thoms]. Chen YG, Qin GW, Xie YY. No
12776320 OBJECTIVE: To study the chemical constituents from the whole plant of Dracocephalum moldavica.METHOD: The compounds were isolated using RA polystyrene resin and silica gel column chromatography, and the structures were elucidated by means of spectral method.RESULT: Four compounds were identified as tilianin, agastachoside, acacetin and oleanolic acid.CONCLUSION: All the compounds were isolated from this plant for the first time. [Studies on chemical constituents from Dracocephalum moldavica L]. Li JB, Ding Y. No
12776330 OBJECTIVE: To study the chemical constituents of Ligularia vellerea.METHOD: The compounds were isolated by column chromatography, and the structures were identified by NMR spectral data and other methods.RESULT AND CONCLUSION: Seven compounds were isolated and identified as 4-hydroxyacetophenone, 8 alpha-hydroxy-7(11)-eremophilen-12, 8 beta-olide, umbelliferone, tiglic acid, 6 beta-hydroxy-7(11)-eremophilen-12, 8 alpha-olide, daucosterin, beta-sitosterol and stigmasterol. All the compounds were isolated for the first time from this plant. [Constituents of Liguliria vellerea (Franch.) Hand-Mazz]. Li YS, Luo SD, Zhang M, Chen JJ, Wang ZT. No
12776331 OBJECTIVE: To study the chemical constituents of Carduus crispus.METHOD: Using chromatographic methods to isolate compounds and using chemical and spectral methods to elucidate the structures of the isolated compounds.RESULT: Eight compounds were elucidated as beta-amyrin palmitate, taraxastery acetate, luteolin-7-O-alpha-L-rhamanopyranosyl-(1-->2)-beta-D-glucopyranoside, luteolin-7-O-beta-D-glucopyranoside, triacontanic acid, beta-sitosterol, stigmasterol and stigmast-7-en-3 beta-ol.CONCLUSION: All the compounds were obtained from this plant for the first time. [Studies on the chemical constituents of Carduus crispus L]. Zhang QY, Wang XY, Ying HP, Cheng TM, Zhao YY. No
12776333 OBJECTIVE: To study the constituents of Callicarpa bodinieri.METHOD: The compounds were isolated by chromatography. Their structures were identified by physical and spectral data.RESULT: Seven compounds were isolated and elucidated as 5-hydroxy-4',3,6,7-tetramethoxy flavone(I), luteolin-7-O-glucoside (II), chrysoeriol-4'-O-glucoside(III), luteolin-4'-O-glucoside(IV), beta-sitosterol(V), ursolic acid(VI), betulinic acid(VII).CONCLUSION: All the compounds were isolated from this plant for the first time; Compounds I, III, IV were isolated from Callicarpa genus for the first time. [Studies on flavonoids from leaves of Callicarpa bodinieri Levl]. Ren FZ, Luan XH, Zhao YM, Qu HH. No
12776359 OBJECTIVE: To study the chemical constituents from Aquilegia ecalcarata.METHOD: Compounds were isolated with silica gel and polyamide chromatography and their structures were determined by spectral analysis and chemical evidence.RESULT: Five compounds were obtained and identified as beta-sitosterol, isoorientin-7-O-glucoside, isovitexin-4'-O-glucoside, isovitexin-2'-O-rhamnoside, luteolin-7-O-glucoside.CONCLUSION: All the compounds were isolated from this plant for the first time. [Studies on the chemical constituents from Aquilegia ecalcarata Maxim]. Chen SB, Gao GY, Wang LW, Liao MC, Yang JS, Xiao PG. No
12776426 OBJECTIVE: To study chemical constituents of the above ground part of Salix oritrepha.METHOD: Using chromatography to isolate compounds and their structure were identified by physical, chemical and spectral techniques.RESULT: Four compounds were isolated and elucidated as beta-sitosterol, beta-sitosteryl-3-O-glucoside, friedelin, luteolin-7-O-glucoside.CONCLUSION: All of them were isolated from S. oritrepha for the first time. Friedelin from the plants of genus Salix for the first time. [Chemical constituents from Salix oritrepha Schneid]. Feng SI, Su C, Duan SY, Chen LR. No
12776495 OBJECTIVE: To study the chemical constituents from the roots and rhizomes of Gerbera piloselloides.METHOD: The compounds were isolated by silica gel column chromatography, and their structures were elucidated by means of spectral analysis.RESULT: Seven compounds were identified as bothrioclinin (I), hydroquinone (II), marmesin (III), succinic acid (IV), umbelliferone (V), isoarborinol (VI) and beta-sitosterol (VII).CONCLUSION: Compound I, V and VI were isolated from Gerbera genus for the first time. [Studies on the chemical constituents from the roots and rhizomes of Gerbera piloselloides]. Xao Y, Li JB, Ding Y. No
12776516 OBJECTIVE: To study the chemical constituents of Ligusticum chuanxiong.METHOD: The constituents were separated and purified by column chromatography with silica gel and ODS, and identified by NMR, MS spectral data.RESULT: Eight compounds were isolated and identified as Ligustilide(I), 4,5-dihydro-3-butylphthalide(II), Z, Z'-6,6',7,3 alpha-diligustili-de(III), Z-6,8',7,3'-diligustilide(IV), Z'-3,8-dihydro-6,6'7,3' alpha-diligustilide(V), 4-hydroxy-3-butylphthalide(VI), ferulic acid(VII) and Xiongterpene(VIII).CONCLUSION: Compound VIII is a new compound. [Studies on chemical constituents of the rhizomae of Ligusticum chuanxiong]. Xiao YQ, Li L, You XL, Taniguchi M, Baba K. No
12781809 Three sterols, 5alpha-ergost-7-en-3beta-ol, 5alpha-ergosta-7,22-dien-3beta-ol and 5,8-epidioxy-5alpha,8alpha-ergosta-6,22-dien-3beta-ol and five triterpenes, applanoxidic acids A, C, F, G and H, have been isolated from Ganoderma annulare. The applanoxidic acids A, C and F were found to inhibit the growth of the fungi Microsporum cannis and Trichophyton mentagrophytes at concentrations of 500 to 1000 microg/ml. Antifungal activity of sterols and triterpenes isolated from Ganoderma annulare. Smania EF, Delle Monache F, Smania A Jr, Yunes RA, Cuneo RS. No
12797740 The essential oils from leaves and inflorescences of Hyptis martiusii Benth were analyzed by GC-MS. Twenty-six compounds representing 93.2% of the essential oil of leaves were characterized; Delta-3-carene (22.5%), 1,8-cineole (24.27%), beta-caryophyllene (6.15%), and bicyclogermacrene (6.32%) were found as the major components. In the essential oil of inflorescences 27 compounds representing 87.7% of the oil were identified. The major components were Delta-3-carene (13.5%), alpha-pinene (5.78%), beta-caryophyllene (6.59%), viridiflorene (8.25%), and germacrene B (5.21%). The essential oil of leaves and 1,8-cineole showed pronounced insecticidal effect against Aedes aegypti larvae and Bemisia argentifolii, the vectors of dengue fever and white fly fruit plague, respectively. Insecticidal activity and chemical composition of volatile oils from Hyptis martiusii Benth. Araújo EC, Silveira ER, Lima MA, Neto MA, de Andrade IL, Lima MA, Santiago GM, Mesquita AL. No
12802722 Using activity against Candida albicans as a lead, the bioactivity-guided fractionation of the dichloromethane extract of Phyllanthus piscatorum led to the isolation of the arylnaphthalide lignan justicidin B (1), which was found to be present in high amounts, and a new C-11 hydroxylated derivative that we named piscatorin (2). We provide evidence that justicidin B (1) is the main active principle of P. piscatorum, showing the same biological effects that were found for the raw extracts. Justicidin B inhibited the growth of the pathogenic fungi Aspergillus fumigatus (MIC > or = 1 microg/mL), Aspergillus flavus (MIC > or = 12 microg/mL), and Candida albicans (MIC > or = 4 microg/mL), but was not effective against Cryptococcus neoformans and Blastoschizomyces capitatus. Justicidin B also exhibited strong activity against the trypomastigote form of Trypanosoma brucei rhodesiense (IC50 = 0.2 microg/mL) and moderate activity against Trypanosoma cruzi (IC50 = 2.6 microg/mL). Testing against Plasmodium falciparum showed only weak activity. This is the first report on the in vitro fungicidal and antiprotozoal effects of justicidin B. In addition, both compounds exhibited a non-specific cytotoxicity in neoplastic and primary cell cultures. No antibacterial effects were detected. Both compounds were piscicidal against zebra fish and it is shown for the first time that piscatorin (2) and justicidin B (1) are the piscicidal principles of P. piscatorum, exhibiting a potency that is comparable to rotenone. Antifungal, antiprotozoal, cytotoxic and piscicidal properties of Justicidin B and a new arylnaphthalide lignan from Phyllanthus piscatorum. Gertsch J, Tobler RT, Brun R, Sticher O, Heilmann J No
12820240 An alkaloidal extract of the stem barks of Zanthoxylum chiloperone var. angustifolium exhibited antifungal activity against Candida albicans, Aspergillus fumigatus and Trichophyton mentagrophytes var. interdigitale using a TLC bioautographic method. Bioassay-guided fractionation of this extract resulted in the isolation of two active compounds identi fi ed as canthin-6-one and 5-methoxycanthin-6-one. Canthin-6-one exhibited a broad spectrum of activities against Aspergillus fumigatus, A. niger, A. terreus, Candida albicans, C. tropicalis, C. glabrata, Cryptococcus neoformans, Geotrichum candidum, Saccharomyces cerevisiae, Trichosporon beigelii, Trichosporon cutaneum and Trichophyton mentagrophytes var. interdigitale with MICs values between 5.3 and 46 micro mol/L. 5-methoxy-canthin-6-one was active against only Trichophyton mentagrophytes var. interdigitale with a MIC value of 12.3 micro mol/L. Antifungal compounds from Zanthoxylum chiloperone var. angustifolium. Thouvenel C, Gantier JC, Duret P, Fourneau C, Hocquemiller R, Ferreira ME, Rojas de Arias A, Fournet A. No
12837370 The methanolic extract and total alkaloids of the aerial parts of Glaucium oxylobum exhibited good activity against Microsporum gypseum, Microsporum canis, Trichophyton mentagrophytes and Epidermophyton floccosum. Four alkaloids, dicentrine, glaucine, protopine, and alpha-allocryptopine, were identified as the compounds responsible for the antifungal activity of this plant. Antifungal activity of the methanolic extract and alkaloids of Glaucium oxylobum Morteza-Semnani K, Amin G, Shidfar MR, Hadizadeh H, Shafiee A. No
12843596 Prenylated xanthones, isolated from the fruit hulls and the edible arils and seeds of Garcinia mangostana, were tested for their antituberculosis potential. Alpha- and beta-mangostins and garcinone B exhibited strong inhibitory effect against Mycobacterium tuberculosis with the minimum inhibitory concentration (MIC) value of 6.25 microg/ml. Tri- and tetra-oxygenated xanthones with di-C5 units or with a C5 and a modified C5 groups are essential for high activities. Substitution in the A and C rings has been shown to modify the bioactivity of the compounds. Antimycobacterial activity of prenylated xanthones from the fruits of Garcinia mangostana. Suksamrarn S, Suwannapoch N, Phakhodee W, Thanuhiranlert J, Ratananukul P, Chimnoi N, Suksamrarn A. No
12889902 [structure: see text] Two types of Daphniphyllum alkaloids with unprecedented fused-hexacyclic ring systems, calyciphyllines A (1) and B (2), have been isolated from the leaves of Daphniphyllum calycinum (Daphniphyllaceae), and the structures and relative stereochemistry were elucidated on the basis of spectroscopic data. Calyciphyllines A and B, two novel hexacyclic alkaloids from Daphniphyllum calycinum. Morita H1, Kobayashi J. No
12895671 Valerian is an ancient tranquillizing drug obtained from the underground organs of several Valeriana species. Its active principles were assumed to be terpenoids in the form of valepotriates and/or as components of the essential oil. However, unknown active compounds were not discarded and synergic effects were suspected. We have recently isolated 6-methylapigenin (MA) from Valeriana wallichii and proved that it is a benzodiazepine binding site (BDZ-bs) ligand [Planta Med. 68 (2002) 934]. The present paper is the first report of the presence of 2S(-)-hesperidin in valeriana and describes that it has sedative and sleep-enhancing properties. MA, in turn, was found to have anxiolytic properties and was able to potentiate the sleep-enhancing properties of hesperidin (HN).MA and HN are new members of the growing family of natural flavonoids with activity on the CNS, and their properties suggest that they are promising drug leads in the field. 6-methylapigenin and hesperidin: new valeriana flavonoids with activity on the CNS Marder M, Viola H, Wasowski C, Fernández S, Medina JH, Paladini AC No
12913233 From the stems of Rubia wallichiana DECNE, thirty-four structurally related compounds were isolated and identified. Three of them, namely rubiawallin-A (1), -B (2), and -C (3), constitute the first report of their occurrence from the natural source. Their structures were determined by comprehensive analyses of their 1D and 2D NMR, and electron impact (EI) mass spectral data. Furthermore, an in vitro screening of cytotoxicity of the isolated compounds was also evaluated. Among the testing compounds, 1-hydroxy-2-hydroxymethyl-3-methoxyanthraquinone (4) demonstrated most effective cytotoxicity towards Hepa-3B and Colo-205 cells. Cytotoxic anthraquinones from the stems of Rubia wallichiana Decne. Wu TS1, Lin DM, Shi LS, Damu AG, Kuo PC, Kuo YH. No
12931858 GC-MS analysis of the essential oil of Shorea robusta bast (cambium + secondary phloem) has revealed the presence of twenty-eight compounds, of which nine compounds, constituting 48.79% of the oil, were identified as T-cadinol (16.75%), alpha-cadinol (16.45%), globulol (4.52%), alpha-copaene (3.79%), gamma-cadinene (2.34%), viridiflorene (1.62%), beta-elemene (1.54%), alpha-terpineol (1.33%) and gamma-muurolene (0.45%). This is the first report on the volatile constituents of the bast which may be significant in influencing host location for Hoplocerambyx spinicornis, the most injurious heartwood borer of Shorea robusta. GC-MS analysis of essential oil of Shorea robusta bast. Kaur S, Varshney VK, Dayal R. No
12932126 Two new xanthone derivatives, garcinianones A (1) and B (2), two new benzophenone derivatives, 4,6,4'-trihydroxy-2,3'-dimethoxy-3-prenylbenzophenone (3) and 4,6,3',4'-tetrahydroxy-2-methoxybenzophenone (4), and a new inseparable mixture of (1E,22Z)-1,22-diferuloyloxydocosane and (1E,24Z)-1,24-diferuloyloxyteracosane (5), together with the previously known 3,8-dihydroxy-2,4,6-trimethoxyxanthone, 6,3'-dihydroxy-2,4-dimethoxybenzophenone, maclurin (6), 2,4,6,3'-tetrahydroxybenzophenone (7), and naringenin, were isolated from the stems of Garcinia multiflora. The structures of 1-5 were elucidated by extensive analysis of their spectral data. Compounds were evaluated in the brine shrimp lethality test and in the DPPH antioxidant assay. Xanthones and benzophenones from the stems of Garcinia multiflora. Chiang YM, Kuo YH, Oota S, Fukuyama Y. No
12932139 Bioassay-guided fractionation resulted in the isolation of four antifungal agents from the roots of Cudrania cochinchinensis. Two of these were new compounds, cudraxanthone S [1, 1,3,5,6-tetrahydroxy-2-(1,1-dimethyl-2-propenyl)xanthone] and cudraflavanone B (2, 2',4',5,7-tetrahydroxy-6-prenylflavanone). The remaining two compounds were known compounds, toxyloxanthone C (3) and wighteone (4). Among these compounds, 1, 3, and 4 exhibited antifungal activities against Cryptococcus neoformans, Aspergillusfumigatus, and A. nidulans (MICs = 2-8 microg/mL). Compounds 1 and 3 also showed antifungal activity against Candida glabrata (MICs = 4-8 microg/mL). Antifungal agents from the roots of Cudrania cochinchinensis against Candida, Cryptococcus, and Aspergillus species. Fukai T, Yonekawa M, Hou AJ, Nomura T, Sun HD, Uno J. No
12943771 Four coumarin derivatives, theraphins A (1), B (2), C (3), and D (4), along with three known xanthones, 2-hydroxyxanthone, 1,7-dihydroxyxanthone, and 5-hydroxy-1-methoxyxanthone, were isolated from the bark of Kayea assamica (Clusiaceae) native to Myanmar. Their structures were determined using spectroscopic and chemical techniques. The absolute configuration of 1 was established by the modified Mosher ester method. Theraphins A (1), B (2), and C (3) exhibited good cytotoxicity against Col2, KB, and LNCaP human cancer cell lines. Theraphin D (4) showed mild activity only against the KB cell line. The coumarins also exhibited mild antimalarial activities. Biologically active alkylated coumarins from Kayea assamica. Lee KH, Chai HB, Tamez PA, Pezzuto JM, Cordell GA, Win KK, Tin-Wa M. No
13524919
Studies on the hypoglycemic and pharmacological actions of some stilbenes HARANATH PS, RANGANATHARAO K, ANJANEYULU CR, RAMANATHAN JD. No
14255131
CHROMATOGRAPHIC INVESTIGATION OF THE ESSENTIAL OIL OF ACHILLEA FRAGRANTISSIMA. SHALABY AF, RICHTER G. No
14531492 The present study was conducted to ascertain the modulatory influences of Ferula asafoetida L. (asafoetida, flavoring agent) on the mammary epithelial tissue differentiation, hepatic drug metabolizing enzymes, antioxidant profiles and N-methyl-N-nitrosourea (MNU)-induced mammary carcinogenesis in Sprague-Dawley rats. Feeding with two doses of asafoetida (1.25 and 2.5% w/w in diet) showed a remarkable increase in the development and differentiation of ducts/ductules (p < 0.01-0.005), lobules (p < 0.005) and a decrease in terminal end buds (p < 0.05-0.005) as compared to both normal and MNU-treated control animals. To assess the biochemical parameters, effect of asafoetida on drug-metabolizing enzymes was evaluated in the liver of rats. Asafoetida treatment significantly reduced (p < 0.05) the levels of cytochrome P450 and b5. There was an enhancement in the activities of glutathione S-transferase (p < 0.05-0.005), DT-diaphorase (p < 0.05-0.01), superoxide dismutase (p < 0.01-0.005) and catalase (p < 0.05-0.005) and in the level of reduced glutathione (p < 0.05-0.005), followed by asafoetida treatment. Also, asafoetida significantly restored the level of antioxidant system, depleted by MNU-treatment. The strengthening of antioxidant system by the lower and higher doses of asafoetida in the presence and absence of MNU was further substantiated by a significant inhibition (p < 0.005) in lipid peroxidation as measured by thiobarbituric acid-reactive substances (TBARS) in the liver of rat. Further, in long-term animal studies, where MNU was used to induce mammary carcinogenesis, asafoetida treatment resulted in a significant reduction in the multiplicity (p < 0.001) and size of palpable mammary tumors (p < 0.005-0.001) and a delay in mean latency period of tumor appearance (p < 0.005). Together, these findings indicate the chemopreventive potential of asafoetida against MNU-induced mammary carcinogenesis. Thus, asafoetida needs further investigation with regard to identification and characterization of its active principle(s) and mechanism of action, for this compound to be developed as a potential chemopreventive agent for human cancers. Chemomodulatory influence of Ferula asafoetida on mammary epithelial differentiation, hepatic drug metabolizing enzymes, antioxidant profiles and N-methyl-N-nitrosourea-induced mammary carcinogenesis in rats. Mallikarjuna GU, Dhanalakshmi S, Raisuddin S, Rao AR. No
14559287 Four new flavonol gycosides: kaempferide 3-O-beta-xylosyl (1-->2)-beta-glucoside, kaempferol 3-O-alpha-rhamnoside-7,4'-di-O-beta-galactoside, kaempferol 3,7,4'-tri-O-beta-glucoside and quercetin 3-O-[alpha-rhamnosyl (1-->6)] [beta-glucosyl (1-->2)]-beta-glucoside-7-O-alpha-rhamnoside, were characterized from a methanolic leaf extract of Warburgia ugandensis. The known flavonols: kaempferol, kaempferol 3-rhamnoside, kaempferol 3-rutinoside, myricetin, quercetin 3-rhamnoside, kaempferol 3-arabinoside, quercetin 3-glucoside, quercetin, kaempferol 3-rhamnoside-4'-galactoside, myricetin 3-galactoside and kaempferol 3-glucoside were also isolated. Structures were established by spectroscopic and chemical methods and by comparison with authentic samples. Flavonol glycosides of Warburgia ugandensis leaves. Manguro LO, Ugi I, Lemmen P, Hermann R. No
14561514 Two prenylated xanthones, 1,4,5,6-tetrahydroxy-7,8-di(3-methylbut-2-enyl)xanthone (1) and 1,2,6-trihydroxy-5-methoxy-7-(3-methylbut-2-enyl)xanthone (2), were isolated from the wood of Garcinia xanthochymus along with a known xanthone, 12b-hydroxy-des-D-garcigerrin A (3). Their structures were elucidated by spectroscopic analysis. Compound 1 (10 muM), 2 (10-30 muM) and 3 (10 muM) showed a markedly enchancing activity of nerve growth factor (NGF)-mediated neurite outgrowth on PC12D cells. Prenylated xanthones with NGF-potentiating activity from Garcinia xanthochymus. Chanmahasathien W, Li Y, Satake M, Oshima Y, Ruangrungsi N, Ohizumi Y. No
14568082 Nine xanthones, nigrolineaxanthones A-I, together with nine known xanthones, were isolated from the crude methanol extract of the stem bark of Garcinia nigrolineata; two of which have previously been reported as synthetic xanthones. The structures were elucidated by analysis of spectroscopic data, especially using 1D and 2D NMR spectroscopic data. Xanthones from the stem bark of Garcinia nigrolineata. Rukachaisirikul V, Ritthiwigrom T, Pinsa A, Sawangchote P, Taylor WC. No
14577632 The water-distilled essential oil the leaves of Coridothymus capitatus were analyzed by GC/MS and also analyzed by direct thermal desorption GC/MS. Comparison was made between two analyses techniques. The essential oil consisted mainly of monoterpenes 98.9%, while oxygenated hydrocarbons were identified as 55.6 % and non-oxygenated hydrocarbons as 43.6%. As major components were found carvacrol (35.6%), p-cymene (21.0%), thymol (18.6%), gamma-terpinene (12.3%), alpha-terpinene (3.2%), beta-myrcene (3.0%) and alpha-thujene (1.3%) by hydrodistillation and by the GC/MS method. The direct thermal desorption GC/MS analysis also showed the same major components, namely carvacrol (51.6%), thymol (21.7%), p-cymene (9.7%) gamma-terpinene (8.2%), alpha-terpinene (1.64%). The essential oil of C. capitatus showed strong activity against S. aureus, P. vulgaris, P. aeruginosa, E. coli, K. pneumonia, B. Analysis of essential oil of Coridothymus capitatus (L.) and its antibacterial and antifungal activity. Goren AC, Bilsel G, Bilsel M, Demir H, Kocabaş EE. No
14595583 The volatile constituents of Achillea clavennae L. (Asteraceae), rare plant of Europe, have been analysed using GC/MS. Twenty- five components making up 81.6% of the oil were characterized with camphor (29.5%), myrcene (5.5%), 1,8-cineole (5.3%), beta-caryophyllene (5.1%) and linalool (4.9%) being the major constituents. The essential oil was evaluated for antibacterial and antifungal activities. The screening of the antimicrobial activity of essential oil was conducted by a disc diffusion test against Gram-positive (Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Streptococcus faecalis), Gram-negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis) and fungal organisms (Aspergillus niger, Aspergillus fumigatus, Candida albicans). The activity was more pronounced against Gram-negative and fungal organisms than against Gram-positive bacteria. A. clavennae oil was found to possess antimicrobial activity against Klebsiella pneumoniae, Pseudomonas aeruginosa and all fungal organisms. Composition and antimicrobial activity of Achillea clavennae L. essential oil. Bezić N, Skocibusić M, Dunkić V, Radonić A. No
14599529 Three pyrones and a 2(5H)-furanone, designated pectinolides D-G, have been isolated from the dichloromethane extract of Hyptis pectinata. The metabolites were characterized on the basis of 1D and 2D NMR spectroscopic techniques. The pyrones were identified as 6S-[3S,6S-(diacetoxy)-5R-hydroxy-1Z-heptenyl]-5S-hydroxy-5,6-dihydro-2H-pyran-2-one (1)- pectinolide D, 6S-[3S,5R,6S-(triacetoxy)-1Z-heptenyl]-5S-acetoxy-5,6-dihydro-2H-pyran-2-one (2)- pectinolide E and 6S-[3S,5R,6S-(triacetoxy)-1Z-heptenyl]-5S-acetoxy-4R-methoxy-3,4,5,6-tetrahydro-4H pyran-2-one (3)- pectinolide F. The furanone was identified as [2'Z,5(1')Z] 5-(4'S,6'R,7'S-triacetoxy-2-octenylidene)-2(5H)-furanone (4)-pectinolide G. Alpha-pyrones and a 2(5H)-furanone from Hyptis pectinata. Boalino DM1, Connolly JD, McLean S, Reynolds WF, Tinto WF. No
14600386 A new prenylated xanthone, 1,3,5,6-tetrahydroxy-4,7,8-tri(3-methyl-2-butenyl)xanthone (1), was isolated from the wood of Garcinia xanthochymus together with a known xanthone, garciniaxanthone E (2). Their structures were determined by spectroscopic analysis. Compounds 1 (3 microM) and 2 (10 microM) elicited marked enhancement of nerve growth factor-mediated neurite outgrowth in PC12D cells Prenylated xanthones from Garcinia xanthochymus. Chanmahasathien W, Li Y, Satake M, Oshima Y, Ishibashi M, Ruangrungsi N, Ohizumi Y. No
14630175 The methanol extract of stem barks of Alianthus excelsa was partitioned with chloroform. The chloroform extract showed fungistatic and fungicidal activity against Aspergillus niger, A. fumigatus, Penicillium frequentence, P. notatum and Botrytis cinerea Antifungal activity of the stem bark of Ailanthus excelsa. Joshi BC, Pandey A, Chaurasia L, Pal M, Sharma RP, Khare A. No
14638393 wenty Thai medicinal plant extracts were evaluated for anti-herpes simplex virus type 1 (HSV-1) activity. Eleven of them inhibited plaque formation of HSV-1 more than 50% at 100microg/ml in a plaque reduction assay. Aglaia odorata, Moringa oleifera, and Ventilago denticulata among the 11 were also effective against thymidine kinase-deficient HSV-1 and phosphonoacetate-resistant HSV-1 strains. These therapeutic efficacies were characterized using a cutaneous HSV-1 infection in mice. The extract of M. oleifera at a dose of 750mg/kg per day significantly delayed the development of skin lesions, prolonged the mean survival times and reduced the mortality of HSV-1 infected mice as compared with 2% DMSO in distilled water (P<0.05). The extracts of A. odorata and V. denticulata were also significantly effective in limiting the development of skin lesions (P<0.05). There were no significant difference between acyclovir and these three plant extracts in the delay of the development of skin lesions and no significant difference between acyclovir and M. oleifera in mean survival times. Toxicity of these plant extracts were not observed in treated mice. Thus, these three plant extracts may be possible candidates of anti-HSV-1 agents. Efficacy of Thai medicinal plant extracts against herpes simplex virus type 1 infection in vitro and in vivo. Lipipun V, Kurokawa M, Suttisri R, Taweechotipatr P, Pramyothin P, Hattori M, Shiraki K. No
14646312 Phytochemical investigation of the whole plant of Andrographis viscosula has led to the isolation of three new 2'-oxygenated flavonoids, (2R)-5-hydroxy-7,2',3'-trimethoxyflavanone (1), 7,2',5'-trimethoxyflavone (2), 5,7,2',3'-tetramethoxyflavone (3), and eight known flavones, 5,7,2'-trimethoxyflavone (4), 5,7,2',4',5'-pentamethoxyflavone (5), echioidinin (6), 5,2',6'-trihydroxy-7-methoxyflavone (7), 5-hydroxy-7,2'-dimethoxyflavone (8), echioidin (9), echioidinin 5-O-glucoside (10), and 5,2',6'-trihydroxy-7-methoxyflavone 2'-O-glucoside (11). The structures of 1-11 were elucidated by physical and spectral methods, including extensive 2D NMR studies. The presence of 2'-oxygenated flavonoids is apparently restricted to Andrographis species in Acanthaceae. Therefore, 2'-oxygenated flavonoids are regarded as chemotaxonomic markers of Andrographis genus in the Acanthaceae family. Flavonoids from Andrographis viscosula. Rao YK, Damu AG, Rao AJ, Venkatesan S, Kuo PC, Rao CV, Wu TS. No
14669259 The antifungal activity of the wogonin isolated from Holmskioldia sanguinea Retz was determined on four different fungal organisms. The pure compound wogonin significantly inhibited the growth of Aspergillus niger, Penicillium frequentance, P. notatum and Botrytis cinerea, in a concentration dependent fashion, when tested by turbidity and spore germination methods. The effects produced by the compound were compared with the standard antifungal agent griseofulvin. Antifungal activity of wogonin. Pal M, Joshi H, Kapoor VP, Pushpangadan P, Chaurasia L. No
14695790 Ten new 1,3,5-trioxygenated xanthones, nigrolineaxanthones J-S (1-10), one new quinone derivative, nigrolineaquinone A (11), and one new isoflavone-like compound, nigrolineaisoflavone A (12), were isolated from the leaves of Garcinia nigrolineata along with four known xanthones and friedelin. Among the xanthones, only nigrolineaxanthone N (5) showed significant antibacterial activity against methicillin-resistant Staphylococcus aureus. Antibacterial xanthones from the leaves of Garcinia nigrolineata. Rukachaisirikul V, Kamkaew M, Sukavisit D, Phongpaichit S, Sawangchote P, Taylor WC. No
14713158 Two new phenylethanoid glycosides integrifoliosides A (2) and B (3), along with a known phenylethanoid glycoside alyssonoside (1) and a flavone glucoside chrysoeriol-7-O-beta-D-glucopyranoside (4) were isolated from the aerial parts of Phlomis integrifolia. The structures of the new compounds were identified as 3,4-dihydroxy-beta-phenylethoxy-O-beta-D-apiofuranosyl-(1 --> 4)-alpha-L-rhamnopyranosyl-(1 --> 3)-4-O-feruloyl-beta-D-glucopyranoside (2) and 3-hydroxy-4-methoxy-beta-phenylethoxy-O-beta-D-apiofuranosyl-(1 --> 4)-alpha-L-rhamnopyranosyl-(1 --> 3)-4-O-feruloyl-beta-D-glucopyranoside (3), on the basis of spectroscopic (UV, IR, 1D- and 2D-NMR, and HR-FABMS) methods. Phenylethanoid glycosides from Phlomis integrifolia Hub.-Mor. Saracoglu I, Varel M, Hada J, Hada N, Takeda T, Donmez AA, Calis I. No
14738396 Four new nortriterpenes, lancifodilactones B-E (1-4), were isolated from the leaves and stems of Schisandra lancifolia. Their structures were determined by analysis of 1D and 2D NMR spectroscopic data. These compounds are members of a new highly oxygenated cycloartane skeletal class with a biosynthetically modified eight-membered ring D. Lancifodilactones B-E, new nortriterpenes from Schisandra lancifolia. Li RT, Xiang W, Li SH, Lin ZW, Sun HD. No
14750030 Two new xanthones, caledonixanthone M 1 and caloxanthone L 2, and one new acid, caledonic acid 6 were isolated from the hexane-soluble extract of the stem bark of Calophyllum caledonicum. In the course of this phytochemical study, seven other known compounds - calothwaitesixanthone, calozeyloxanthone, allanxanthone, isoapetalic acid 3, calolongic acid 4, apetalic acid 5 and isocalolongic acid 7 - were isolated. Their antifungal activity against the growth of the human pathogenic fungus Aspergillus fumigatus was then investigated. The results indicated that the crude extract, calolongic acid 4 and isocalolongic acid 7 exhibited strong inhibitory effects with MIC (80) values of 8, 4, 2 microg/mL, respectively. Besides, calolongic acid 4, its lactone derivative 4a and isocalolongic acid 7 markedly reduced the respiration of pea seed mitochondria Antifungal chromans inhibiting the mitochondrial respiratory chain of pea seeds and new xanthones from Calophyllum caledonicum. Hay AE, Guilet D, Morel C, Larcher G, Macherel D, Le Ray AM, Litaudon M, Richomme P. No
14753677 Hydrodistillation (HD), simultaneous distillation-solvent extraction (SDE), microwave-assisted hydrodistillation (MWHD), and supercritical fluid (CO2) extraction (SFE), were employed to isolate volatile secondary metabolites from Colombian Xylopia aromatica (Lamarck) fruits. Static headspace (S-HS), simultaneous purge and trap (P&T) in solvent (CH2Cl2), and headspace (HS) solid-phase microextraction (SPME) were utilised to obtain volatile fractions from fruits of X. aromatica trees, which grow wild in Central and South America, and are abundant in Colombia. Kováts indices, mass spectra or standard compounds, were used to identify more than 50 individual components in the various volatile fractions. beta-Phellandrene was the main component found in the HD and MWHD essential oils, SDE and SFE extracts (61, 65, 57, and ca. 40%, respectively), followed by beta-myrcene (9.1, 9.3, 8.2 and 5.1%), and alpha-pinene (8.1, 7.3, 8.1 and 5.9%). The main components present in the volatile fractions of the X. aromatica fruits, isolated by S-HS, P&T and HS-SPME were beta-phellandrene (53.8, 35.7 and 39%), beta-myrcene (13.3, 12.3 and 10.1%), p-mentha-1(7),8-diene (7.1, 10.6 and 10.4%), alpha-phellandrene (2.2, 5.0 and 6.4%), and p-cymene (2.2,4.7 and 4.4%), respectively. Analysis of volatile secondary metabolites from Colombian Xylopia aromatica (Lamarck) by different extraction and headspace methods and gas chromatography. Stashenko EE, Jaramillo BE, Martínez JR. No
14964671 Assafoetida is an oleo-gum resin obtained from the roots and stems of many Ferula species such as F. foetida Regel, F. asafoetida Linn, F. alliacea Boiss. and F. narthex Boiss, family Umbelliferae (carrot family), plants growing indigenously in Iran, Afghanistan and north of India. Assafoetida is commonly used as a flavoring agent in food and as a traditional medicine for many diseases in many parts of the world. In this work, the effect of Assafoetida on growth of Trichomonas vaginalis in vitro was studied and compared to metronidazole as a reference drug. It showed that Assafoetida had a potent antiparasitic effect on T. vaginalis compared to metronidazole, thus, worth further investigation to study its applicability in treatment of parasitic infections. The in vitro effect of Assafoetida on Trichomonas vaginalis. Ramadan NI, Al Khadrawy FM. No
14984086 Five constituents including one new pentacyclic triterpenoid guajanoic acid (1) and four known compounds beta-sitosterol (2), uvaol (3), oleanolic acid (4), and ursolic acid (5) have been isolated from the leaves of Psidium guajava. The new constituent 1 has been characterized as 3beta-p-E-coumaroyloxy-2alpha-methoxyurs-12-en-28-oic acid through 2D NMR techniques and chemical transformations. This is the first report of isolation of compound 3 from the genus Psidium. Chemical constituents from the leaves of Psidium guajava. Begum S, Hassan SI, Ali SN, Siddiqui BS. No
15015374 OBJECTIVE:To examine the saponins constituents in roots of Panax quinquefolium cultivated in China.METHOD:The methanol extract from roots of P. quinquefolium cultivated in Jiling province of China was extracted by chloroform and n-butanol successively. Ten pure saponins were isolated from the n-butanol extract by silica gel and RP-8 reversed-phase column chromatography. Their structures were identified by means of spectral methods and compared with known compounds.RESULT:Ten saponins were isolated from P. quinquefolium. They were identified as ginsenoside Rg1(2), Re(5), Rd(7), Rc(8), Rb1(9), Rb2(10), 24(R)-ginsenoside Rg3(3), 24(R)-pseudoginsenoside RT5(1), F11(4) and notoginsenoside K(6), respectively.CONCLUSION:This work has elucidated the saponins constituents of P. quinquefolium cultivated in Jilin province of China and has shown that compound 1 was isolated from this plant for the first time. [Studies on saponin constituents in roots of Panax quinquefolium]. Su J1, Li HZ, Yang CR. No
15015375 OBJECTIVE: To study chemical constituents of Ilex kudingcha.METHOD: The constituents were isolated by chromatographic method and the structures were identified on the basis of spectral analysis.RESULT: Seven compounds, lupeol(I); 3 beta-hydroxyl-lup-20(29)-ene-24-methyl ester(II); lup-20(29)-ene-3 beta-, 24-diol(III); beta-sitosterol(IV); ursolic acid(V); daucosterol (VI); mannitol were obtained(VII).CONCLUSION: I, III, VI and VII compounds were obtained from genus Ilex for the first time. [Studies on chemical constituents in leafs of Ilex kudingcha]. Liu S, Qin Y, Du FL. No
15022161 The phytochemicals betulinic acid (1), wogonin (2) and oroxindin (3) isolated from the aerial parts of Bacopa monnieri and Holmskioldia sanguinea showed significant antifungal activity against the two fungi Alternaria alternata and Fusarium fusiformis. Inhibition of root growth germination of wheat seeds was observed for all three compounds which showed 100% inhibition at 10 micro g/mL. Compounds (1) and (2) showed potent inhibition of Alternaria alternata compared with oroxindin at a concentration of 4 micro g/mL, whereas compound (2) was an effective inhibitor of both fungi. The structures of the compounds were established by spectral and chemical studies. Phytotoxic and antimicrobial constituents of Bacopa monnieri and Holmskioldia sanguinea. Chaudhuri PK, Srivastava R, Kumar S, Kumar S. No
15040088 AIM: To study the active constituents of Swertia davidi Franch. METHODS: Chemical components were isolated by column chromatography and their structures were established mainly by spectroscopic means (UV, IR, NMR, 2D-NMR, MS). RESULTS: Three substances were identified as 2,5-dimethoxyl-1, 4-dicarboxyl benzene (VIII), 1,5,8-trihydroxyl-3,4-dimethoxyl xanthone (IX) and 1,8-dihydroxyl-3-(3'-hydroxyl-butoxy) xanthone (X). CONCLUSION: Compounds VIII and IX were isolated from Swertia davidi Franch, for the first time, whereas compound X is a new xanthone, named daviditin B with antioxygenated activity in vitro. [Daviditin B from Swertia davidi Franch]. Tan GS, Xu KP, Li FS, Xu PS, Hu GY, Jiang DJ, Li YJ. No
15040089 AIM: To study the chemical constituents of the seeds of Ziziphus jujuba Mill var. spinosa (Bunge) Hu ex. H.F. Chou.

METHODS:

To separate the constituents by using various kinds of chromatography methods and identify their structures on the basis of spectral analysis.

RESULTS:

Seven compounds were isolated. Their structures were established as jujuboside E (1), jujuboside B (2), jujuboside A (3), betulic acid (4), stearic acid (5), sucrose (6) and inosine (7).

CONCLUSION:

Compound 1 is a new compound named jujuboside E. Compounds 5, 6, 7 were isolated for the first time from this plant.
Structure identification of jujuboside E Bai YJ, Cheng G, Tao J, Wang B, Zhao YY, Liu Y, Ma LB, Tu GZ No
15056964 A new compound, nigeglanine (1), and its new artificial derivative (1a), were isolated from the seeds of Nigella glandulifera, together with a known aporphine alkaloid, fuzitine (2). Their structures were established by spectral analysis, including two-dimensional (2D)-NMR spectroscopy. Nigeglanine (1) is the third natural product determined to contain an indazole nucleus. A new alkaloid and its artificial derivative with an indazole ring from Nigella glandulifera. Liu YM1, Yang JS, Liu QH. No
15074599 Essential oils obtained by steam distillation from the aerial parts of Achillea fragrantissima (Forssk.) Sch. Bip. and the flower, leaf and stem of A. santolina L. as well as their lipophilic constituents obtained by solvent extraction were analysed using GLC and GLC-mass spectrometry. Nineteen constituents in the essential oil of A. fragrantissima were identified, in addition to 41 compounds from its n-hexane-ether extract. The hydrodistilled oil and the solvent extract contain santolina alcohol, artemisia alcohol, artemisia ketone, cis-thujone and trans-thujone as major constituents. In A. santolina altogether 54 volatile components were detected. The major components were 1,8-cineole, fragranol, fragranyl acetate and terpin-4-ol. Furthermore, the essential oils and the n-hexane-ether extracts of the two plants were screened for their antimicrobial activity. Comparative study of the essential oils and extracts of Achillea fragrantissima (Forssk.) Sch. Bip. and Achillea santolina L. (Asteraceae) from Egypt. el-Shazly AM, Hafez SS, Wink M. No
15081299 From the quaternary alkaloidal fraction of the bark and the root of Xylopia parviflora (Annonaceae), four isoquinoline alkaloids, xylopinidine, dehydrocoreximine, N, N-dimethylanomurine and N-methylphoebine were isolated along with the known compounds, pycnarrhine, lotusine, 6,7-dimethoxy-2-methyl-isoquinolinium salt, 1,2-dehydroreticuline, (-)-phellodendrine, (+)-tembetarine, (-)-litcubine, (+)-magnoflorine, tetradehydroreticuline, (-)-oblongine, (+)-menisperine, (+)-N-methylcorydine, stepharanine, (+)-xanthoplanine, dehydrodiscretine, jatrorrhizine and palmatine. 3,4-Dihydro-6,7-dimethoxy-2-methyl-isoquinolinium and N-methylpurpuerine were isolated as natural products for the first time. Their structures were determined on the basis of spectroscopic evidence. Quaternary isoquinoline alkaloids from Xylopia parviflora. Nishiyama Y1, Moriyasu M, Ichimaru M, Iwasa K, Kato A, Mathenge SG, Chalo Mutiso PB, Juma FD. No
15104511 Two new xanthones, 6-O-methyl-2-deprenylrheediaxanthone B (1) and vieillardixanthone (2), were isolated from the stem bark of Garcinia vieillardii, as were four known compounds (4-7). The structures of 1 and 2 were determined by means of spectroscopic analysis and chemical derivatization. Each isolate was tested for its antioxidant properties based on a scavenging activity study using the stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. Antioxidant xanthones from Garcinia vieillardii. Hay AE, Aumond MC, Mallet S, Dumontet V, Litaudon M, Rondeau D, Richomme P. No
15124093 Nine new triterpenoid saponins were isolated from the bulbs of Bolbostemma paniculatum (Maxim.) Franquet (Cucurbitaceae): 7beta,18,20,26-tetrahydroxy-(20S)-dammar-24 E-en-3-O-alpha-L-(3-acetyl)arabinopyranosyl-(1-->2)-beta-D-glucopyranoside, 7beta,18,20,26-tetrahydroxy-(20S)-dammar-24E-en-3-O-alpha-L-(4-acetyl)arabinopyranosyl-(1-->2)-beta-D-glucopyranoside, 7beta,18,20,26-tetrahydroxy-(20S)-dammar-24E-en-3-O-alpha-L-arabinopyranosyl-(1-->2)-beta-D-(6-acetyl)glucopyranoside, 7beta,20,26-trihydroxy-(20S)-dammar-24E-en-3-O-alpha-L-arabinopyranosyl-(1-->2)-beta-D-glucopyranoside, 7beta,20,26-trihydroxy-(20S)-dammar-24E-en-3-O-alpha-L-(3-acetyl)arabinopyranosyl-(1-->2)-beta-D-glucopyranoside, 7beta,20,26-trihydroxy-(20S)-dammar-24E-en-3-O-alpha-L-(4-acetyl)arabinopyranosyl-(1-->2)-beta-D-glucopyranoside, 7beta,20,26-trihydroxy-8-formyl-(20S)-dammar-24E-en-3-O-alpha-L-(3-acetyl)arabinopyranosyl-(1-->2)-beta-D-glucopyranoside, 7beta,20,26-trihydroxy-8-formyl-(20S)-dammar-24E-en-3- O-alpha-L-(4-acetyl)arabinopyranosyl-(1-->2)-beta-D-glucopyranoside and 6'-O-palmitoyltubeimoside I. In addition, four known triterpenoid saponins: tubeimoside I, tubeimoside II, tubeimoside III and tubeimoside IV were isolated. The structures of the above compounds were elucidated based on spectroscopic studies, and the configuration of C-20 of tubeimoside IV was revised as S rather than R as reported in previous literature. The compounds were tested for their antiviral activity New triterpenoid saponins from bulbs of Bolbostemma paniculatum. Liu WY1, Zhang WD, Chen HS, Gu ZB, Li TZ, Chen WS. No
15125576 A new steroidal saponin, dioscoreside E (1), and a known compound, protodioscin (2), were isolated from an ethanol extract of the rhizomes of Dioscorea panthaica. The structure of 1 was established as 3-O-[bis-alpha-L-rhamnopyranosyl-(1 --> 2 and 1 --> 4)-beta-D-glucopyranosyl]-26-O-beta-D-glucopyranosyl-20(R)-methoxy-25(R)-furosta-5,22(23)-diene-3beta,26-diol, on the basis of spectral and chemical evidence. Compounds 1 and 2 showed cytotoxic activity against a panel of tumor cell lines. Steroidal saponins from Dioscorea panthaica and their cytotoxic activity. Dong M1, Feng XZ, Wang BX, Ikejima T, Wu LJ. No
15133212 The dimeric butenolides glochidiolide and isoglochidiolide, a new glucoside of a nitrogen-containing C(8) dimer, acuminaminoside, and glucosides of C(8) compounds, designated glochidacuminosides A-D, were isolated from the leaves of Glochidion acuminatum. The structures of glochidionolactone and acuminaminoside were determined by X-ray analyses, and those of the remaining C(8) glucosides by NMR spectroscopic analyses, chemical conversion, and a modified Mosher's method. Glochidiolide, isoglochidiolide, acuminaminoside, and glochidacuminosides A-D from the leaves of Glochidion acuminatum MUELL. Otsuka H1, Takeda Y, Hirata E, Shinzato T, Bando M. No
15138013 The objective of this study was to investigate the inhibitory effect of the crude extracts from some herbs on adherence of Streptococcus mutans (S. mutans) ATCC 25175 and TPF-1 in vitro. Six herbs, Andrographis paniculata; Cassia alata; Chinese black tea (Camellia sinensis); guava (Psidium guajava); Harrisonia perforata and Streblus asper, were extracted with 50 or 95% ethanol and dried. Herbal extracted solution at 0.5% concentration (w/v) was initially tested for bacterial adherence on glass surfaces. In order to identify type and effective concentration of the extracts, the extracts that showed the inhibition on glass surfaces were then tested on saliva-coated hydroxyapatite by the use of radiolabeled bacteria. To study the mechanism of action, the effect of the extracts at such concentration on glucosyltransferase and glucan-binding lectin activities were examined. It was found that all extracts, but Streblus asper, showed significant inhibitory effect on bacterial adherence to glass surfaces. For the saliva-coated hydroxyapatite adherence assay, Andrographis paniculata, Cassia alata, Chinese black tea and Harrisonia perforata could inhibit adherence of S. mutans ATCC 25175. Chinese black tea was the strongest inhibitor followed by Andrographis paniculata, Cassia alata and Harrisonia perforata, respectively. For S. mutans TPF-1, adherence inhibition was observed from Andrographis paniculata and Cassia alata at similar levels. The lowest concentrations of the extracts that inhibited the adherence at least 50% were 0.5% of Andrographis paniculata, 0.5% of Cassia alata, 0.3% of Chinese black tea and 0.5% of Harrisonia perforata for S. mutans ATCC 25175. For S. mutans TPF-1, the effective concentrations were 0.5% of Andrographis paniculata and 0.4% of Cassia alata. All extracts at such concentrations decreased the activity of glucosyltransferase from both strains. Only Andrographis paniculata and Cassia alata eliminated or decreased the activity of glucan-binding lectin from both strains. These findings suggested that Andrographis paniculata, Cassia alata, Chinese black tea and Harrisonia perforata could inhibit adherence of S. mutans ATCC 25175, while Andrographis paniculata and Cassia alata had an effect on S. mutans TPF-1 in vitro at the concentrations employed in this study. Inhibitory effect of some herbal extracts on adherence of Streptococcus mutans. Limsong J, Benjavongkulchai E, Kuvatanasuchati J. No
15139128 OBJECTIVE: To investigate the chemical constituents of Chinese herb Melastoma dodecandrum.METHOD: The chemical constituents were isolated by solvent extraction and chromatography. Their structure were identified on the basis of physic-chemical constants and specral data.RESULT: Five compounds were isolated and identified as hexacosanoic acid (I), quercetin(II), avicularin(III), gallic acid (IV), kaempferol(V).CONCLUSION: The above-mentioned compounds were separated from the plant for the first time. [Studies on the chemical constituents of Chinese herb Melastoma dodecandrum]. Zhang C, Fang YX. No
15165151 Two new quassinoids, javanicolides C and D, and five new quassinoid glucosides, javanicosides B-F, were isolated from the seeds of Brucea javanica, along with eight known quassinoids, i.e., yadanziolides A, C, D, and S, bruceins D and E, brusatol, and the aglycone of yadanzioside D, and 19 known quassinoid glucosides, i.e., yadanziosides A-G, I, and K-P, bruceosides A-C and E, and bruceantinoside A. Their structures were elucidated by analysis of spectroscopic data and chemical evidence New quassinoids, javanicolides C and D and javanicosides B--F, from seeds of Brucea javanica. Kim IH1, Takashima S, Hitotsuyanagi Y, Hasuda T, Takeya K. No
15165154 Two new 18(13-->12)-abeo-lanostane triterpenoid acids, ananosic acids B (1) and C (2), were isolated from the stems of Kadsura anaosma. Their structures were elucidated by spectral studies and chemical transformation. Compounds 1 and 2 were evaluated for cytotoxicity using CCRF-CEM leukemia cells and HeLa cells. Ananosic acids B and C, two new 18(13-->12)-abeo-lanostane triterpenoids from Kadsura ananosma. Chen YG, Hai LN, Liao XR, Qin GW, Xie YY, Halaweish F. No
15229808 Among the different xanthones previously isolated from the stem bark of Calophyllum caledonicum, caledonixanthone E presented the strongest activity (MIC (80) = 8 microg/mL) in acidic conditions (pH 3) against the human pathogenic fungus Aspergillus fumigatus. Phase-contrast microscopy studies suggested the assembly or synthesis of cell wall components as the target of the drug. Moreover, the use of fluorescent lectins further supported an impact of caledonixanthone E on the synthesis of chitin, the major structural polysaccharide of the fungal wall. These results suggest that caledonixanthone E may be an interesting model for the design of new antifungal drugs Investigation of the antifungal activity of caledonixanthone E and other xanthones against Aspergillus fumigatus. Larcher G, Morel C, Tronchin G, Landreau A, Séraphin D, Richomme P, Bouchara JP. No
15270578 A new homoerythrina alkaloid, C-3-epi-wilsonione (1), a new tetraflavonoid, taiwanhomoflavone C (2), and a new stereoisomer of desmethylcephalotaxinone (3) have been isolated from the leaves and heartwood of Cephalotaxus wilsoniana, respectively. The structures were elucidated by spectroscopic methods. Compound 1 showed cytotoxic activity against a number of human cancer cell lines in vitro. New alkaloids and a tetraflavonoid from Cephalotaxus wilsoniana. Wang LW, Su HJ, Yang SZ, Won SJ, Lin CN. No
15279971 Four new clerodane furano diterpene glucosides (amritosides A, B, C and D) were isolated as their acetates from Tinospora cordifolia stems. The structures of these compounds were established on the basis of spectroscopic studies. Amritosides A, B, C and D: clerodane furano diterpene glucosides from Tinospora cordifolia. Maurya R1, Manhas LR, Gupta P, Mishra PK, Singh G, Yadav PP. No
15279978 From the aerial parts of Sideritis ozturkii, three new flavonoids, chrysoeriol 7-O-[2'''-O-caffeoyl-O-acetyl-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside], chrysoeriol 7-O[2'''-O-caffeoyl-beta-D-glucopyranosyl-(1 -->2)-beta-D-glucopyranoside] and chrysoeriol 7-O[2'''-O-p-coumaroyl-6'''-beta-O-acetyl-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside] named as ozturkosides A, B and C, respectively, were isolated, along with three known phenylethanoid glycosides, verbascoside, leucoseptoside A, martynoside and five known diterpenoids, 7-epicandicandiol, linearol, sidol, sideroxol, epoxyisolinearol. The structures were elucidated mainly by spectroscopic methods. Three acylated flavone glycosides from Sideritis ozturkii Aytac & Aksoy. Sahin FP, Taşdemir D, Rüedi P, Ezer N, Caliş I. No
15279983 Aerial parts of a collection of Delphinium pentagynum Lam. from Niebla, Southern Spain, furnished one diterpene alkaloid, 2-dehydrodeacetylheterophylloidine, two norditerpene alkaloids, 14-demethyl-14-isobutyrylanhweidelphinine and 14-demethyl-14-acetylanhweidelphinine, the known alkaloids 14-deacetylnudicauline, methyllycaconitine, 14-deacetyl-14-isobutyrylnudicauline, 14-acetylbrowniine, browniine, delcosine, lycoctonine, 18-methoxygadesine, neoline, karakoline and the aporphine alkaloid magnoflorine. Structures of the alkaloids were established by MS, 1D and 2-D NMR techniques. Alkaloids from Delphinium pentagynum. Díaz JG, Ruiz JG, Herz W. No
15376387 OBJECTIVE: To study the chemical constituents from Buddleja purdomii W. W. Smith.METHODS: The constituents were isolated and purified by various chromatographic methods and structurally identified by spectral analysis.RESULTS: 4 compounds were identified as vanillin (I), vanillic acid (II), acteoside (III), acteoside isomer (IV).CONCLUSION: All these compounds were obtained from this plant for the first time. [Study on the chemical constituent from Buddleja purdomii]. Gao Y, Li C, Zhang C, Xu Y, Tao B. No
15376388 OBJECTIVE: To study the chemical constituents from Eucommia ulmoides Oliv.METHODS: The chemical constituents were isolated and repeatedly purified on silica gel column. They were identified and structurally elucidated by means of physio-chemical constants and spectral analysis.RESULTS: Seven compounds were obtained. They were (-)epicatechin (I), catechin (II), n-octacosanoic acid (III), tetracosanoic-2, 3-dihydroxypropylester (IV), rutin (V), chlorogenic acid (VI), and caffeic acid (VII), respectively.CONCLUSION: Four compounds (II, III, IV, V) were isolated from bark of Eucommia ulmoides Oliv for the first time. [Studies on chemical constituents from Eucommia ulmoides Oliv]. Sun Y, Dong J, Wu S. No
15387663 Chemical investigation of the CHCl(3) extracts from the roots of Delphinium scabriflorum has resulted in the isolation of a new diterpenoid alkaloid, 13-(2-methylbutyryl)azitine (1), along with 11 known alkaloids, delbine (2), 14-deacetyl-14-isobutyrylajadine (3), methyllycaconitine (4), 14-deacetylnudicauline (5), delectinine (6), deltatsine (7), dictysine (8), geyerline (9), ajacine (10), lycoctonine (11), and delcosine (12). The structure of 1 was determined by spectroscopic data interpretation. Complete NMR data for alkaloids 2-8 are presented. Some earlier (13)C NMR assignments made for alkaloids 4-7 were revised. Diterpenoid alkaloids from the roots of Delphinium scabriflorum. Shrestha PM, Katz A. No
15387668 A new indole alkaloid, akuammiginone (1), and a new glycosidic indole alkaloid, echitamidine-N-oxide 19-O-beta-d-glucopyranoside (2), together with the five known alkaloids, echitaminic acid (3), echitamidine N-oxide (4), N(b)-demethylalstogustine N-oxide (5), akuammicine N-oxide (6), and N(b)-demethylalstogustine (7), were isolated from the trunk bark of Alstonia scholaris collected in Timor, Indonesia. The structures of all compounds were elucidated by spectroscopic methods. This is the first report of compounds 3-5and 7 in A. scholaris. Some NMR assignments of the known compounds were revised. New indole alkaloids from the bark of Alstonia scholaris. Salim AA1, Garson MJ, Craik DJ. No
15451323 Xanthones, 1,3-dihydroxy-5-methoxyxanthone-4-sulfonate and 1,3-dihydroxy-5-O-beta-D-glycopyranosylxanthone-4-sulfonate, together with nine known compounds were obtained from H. sampsonii. This is the first report of sulfonated xanthonoids. Furthermore, compounds 1 and 2 exhibited significant cytotoxicity against the P388 cancer cell line. Sulfonated xanthones from Hypericum sampsonii. Hong D, Yin F, Hu LH, Lu P. No
15474559 The antimalarial activity of 22 xanthones against chloroquino-resistant strains of Plasmodium falciparum was evaluated. Natural caloxanthone C (1), demethylcalabaxanthone (2), calothwaitesixanthone (3), calozeyloxanthone (4), dombakinaxanthone (5), macluraxanthone (6), and 6-deoxy-gamma-mangostin (7) were isolated from Calophyllum caledonicum. 1,6-dihydroxyxanthone (8), pancixanthone A (9), isocudraniaxanthone B (10), isocudraniaxanthone A (11), 2-deprenylrheediaxanthone B (12) and 1,4,5-trihydroxyxanthone (13) were isolated from Garcinia vieillardii. Moreover, synthetic compounds (14-22) are analogues or intermediates of xanthones purified from Calophyllum caledonicum (Oger J.M., Morel C., Helesbeux J.J., Litaudon M., Seraphin D., Dartiguelongue C., Larcher G., Richomme P., Duval O. 2003. First 2-Hydroxy-3-Methylbut-3-Enyl substituted xanthones isolated from Plants: structure elucidation, synthesis and antifungal activity. Natural Product Research 17(3), 195-199; Helesbeux J.J., Duval O., Dartiguelongue C., Seraphin D., Oger J.M., Richomme P., 2004. Synthesis of 2-hydroxy-3-methylbut-3-enyl substituted coumarins and xanthones as natural products. Application of the Schenck ene reaction of singlet oxygen with ortho-prenylphenol precursors. Tetrahedron 60(10), 2293-2300). The relationship between antimalarial activity and molecular structure of xanthones has also been explored. The most potent xanthones (2), (3) and (7) (IC50 = c.a. 1.0 microg/mL) are 1,3,7 trioxygenated and prenylated on the positions 2 and 8. Antimalarial xanthones from Calophyllum caledonicum and Garcinia vieillardii. Hay AE, Hélesbeux JJ, Duval O, Labaïed M, Grellier P, Richomme P. No
15476301 Screening was done of some plants of importance in the Ayurvedic system of traditional medicine used in India to treat enteric diseases. Fifty four plant extracts (methanol and aqueous) were assayed for their activity against multi-drug resistant Salmonella typhi. Strong antibacterial activity was shown by the methanol extracts of Aegle marmelos, Salmalia malabarica, Punica granatum, Myristica fragrans, Holarrhena antidysenterica, Terminalia arjuna and Triphal (mixture of Emblica of fi cinalis, Terminalia chebula and Terminalia belerica). Moderate antimicrobial activity was shown by Picorhiza kurroa, Acacia catechu, Acacia nilotica, Cichorium intybus, Embelia ribes, Solanum nigrum, Carum copticum, Apium graveolens, Ocimum sanctum, Peucedanum graveolens and Butea monosperma. Antimicrobial evaluation of some medicinal plants for their anti-enteric potential against multi-drug resistant Salmonella typhi. Rani P, Khullar N. No
15478193 The antibacterial and antifungal activities of methanol extract, ethyl acetate, dichloromethane, n-hexane, n-butanol and chloroform fractions of Senecio inaequidens DC. and Senecio vulgaris L. were assayed. The hexane extract of Senecio vulgaris L. (MIC 0.031 mg/ml) showed significant activity against Trichophyton tonsurans Antibacterial and antifungal activity of Senecio inaequidens DC. and Senecio vulgaris L. Loizzo MR, Statti GA, Tundis R, Conforti F, Bonesi M, Autelitano G, Houghton PJ, Miljkovic-Brake A, Menichini F No
15497949 Six new xanthones, drimiopsins A-F (1-6), have been isolated from the South African Drimiopsis maculata. The structures of these compounds were difficult to elucidate due to the lack of correlating protons seen in the NMR spectra, and INADEQUATE spectra were used to confirm the structures. Xanthones have not previously been reported from the Hyacinthaceae. Xanthones from Drimiopsis maculata. Mulholland DA, Koorbanally C, Crouch NR, Sandor P. No
15501261 Two xanthones, namely virgataxanthone A and B, have been isolated from the stem bark of Garcinia virgata, together with two formylated tocotrienols and the known delta-tocotrienol, griffipavixanthone and 2,6-dihydroxy-4-methoxybenzophenone (cotoin). Their structures were mainly established using one and two-dimensional NMR and mass spectroscopies. When sufficient material was available, the antioxidant activities of the crude extracts as well as the isolated compounds were evaluated. Prenylated xanthones and tocotrienols from Garcinia virgata. Merza J, Aumond MC, Rondeau D, Dumontet V, Le Ray AM, Séraphin D, Richomme P. No
15501264 Seven new steroidal glycosides named pentandrosides A(1)-G(7) were isolated from the EtOH extract of the aerial parts of Tribulus pentandrus. Pentandrosides A(1)-E(5) possess cholestane aglycones, pentandroside F(6) a furostan-type aglycone and pentandroside G(7) an unusual acyloxypregnane aglycone probably derived from the degradation of a furostan skeleton. Structure elucidation of 1-7 was accomplished through the extensive use of 1D- and 2D NMR experiments including 1H-1H (DQF-COSY, 1D-TOCSY) and 1H-13C (HSQC, HMBC) spectroscopy along with ESIMS and HRESIMS. Steroidal saponins from the aerial parts of Tribulus pentandrus Forssk. Hamed AI1, Oleszek W, Stochmal A, Pizza C, Piacente S. No
15541744 Sixteen more Amaryllidaceae alkaloids have been isolated from bulbs of Crinum kirkii Baker of which noraugustamine and 4a,N-dedihydronoraugustamine are hitherto unknown. Their structures and those of earlier known alkaloids have been established by physical and spectroscopic analysis. Application of 2D NMR techniques was used for complete characterization of the alkaloids as well as of 3-O-acetylsanguinine. 1,2-Diacetyllycorine and 3-O-acetylsanguinine showed activity against Trypanosoma brucci rhodesiense, the parasite associated with sleeping sickness. 3-O-acetylsanguinine also showed some activity against Trypanosoma cruzi. Augustamine type alkaloids from Crinum kirkii. Machocho AK1, Bastida J, Codina C, Viladomat F, Brun R, Chhabra SC. No
15541747 Quassinoid glucosides, javanicosides I, J, K and L, were isolated from the seeds of Brucea amarissima (Lour.) Desv. ex B. A. Gomes (Simaroubaceae), along with two known quassinoids, i.e. bruceins D and E, and seven known quassinoid glucosides, yadanziosides B, C, E, I and K, bruceoside B and yadanzigan. Their structures were elucidated by analysis of the spectral data and chemical evidence. Quassinoid glucosides from seeds of Brucea amarissima. Kim IH1, Hitotsuyanagi Y, Takeya K. No
15563060 AIM: To study the chemical constituents of Ziziphus jujuba Mill var. Spinosa (Bunge) Hu ex. H. F. Chou. Structure identification of jujuboside D Liu QX, Wang B, Liang H, Zhao YY, Liu MJ No
15575205 OBJECTIVE: To investigate the chemical constituents of the dried buds of Lonicera confusa.METHOD: Chromatography and spectral analysis were respectively used to isolate and identify the constituents.RESULT: Seven compounds were isolated from the dried buds of L. confusa, and identified as rutin, quercetin, luteilin-7-O-beta-D-galactoside, lonicerin, chlorogenic acid, beta-sitosterol and tetratriacontane.CONCLUSION: Rutin was isolated from the genus for the first time, and the others were isolated from the species for the first time. [Studies on chemical constituents in dried buds of Lonicera confusa]. Chai XY, Li P, Tang LY. No
15577257 From the stem bark of Albizzia julibrissin DURAZZ (Leguminosae), two new phenolic glycosides (albibrissinosides A and B) were isolated. Their structures were determined by spectroscopic analysis. The albibrissinoside B was found to be a radical scavenger on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. Phenolic glycosides from the stem bark of Albizzia julibrissin. Jung MJ, Kang SS, Jung YJ, Choi JS. No
15587597 Two new alkaloids, namely 8-hydroxylycorin-7-one and 2-deoxylycorine were isolated from Crinum bulbispermum along with the known compounds vittatine, 11-hydroxyvittatine and hippamine. Alkaloids from the bulbs of Crinum bulbispermum. Aboul-Ela MA1, El-Lakany AM, Hammoda HM. No
15606029 AIM:To study the chemical constituents of Polygala aureocauda Dunn..METHODS: Chemical compounds were isolated by column chromatography and their structures were determined mainly by spectroscopic means (UV, IR, MS, 1HNMR, 13CNMR, HMQC, HMBC).RESULTS: Three compounds were isolated and identified as 3-hydroxy-1,4-dimethoxyxanthone (I), 1, 7-dihydroxy-2, 3-methylendioxyxanthone (II), 7-hydroxy-1-methoxy-2, 3-methylendioxyxanthone (III).CONCLUSION: Compounds I-III were isolated from Polygala aureocauda Dunn. for the first time, whereas compound I is a new xanthone. [A new xanthone from Polygala aureocauda Dunn]. Huang ZH, Xu KP, Zhou YJ, Hu GY, Tan GS. No
15620237 A new furofuran lignan, styraxlignolide B (1), and four new dibenzyl-gamma-butyrolactone lignans, styraxlignolides C-F (2-5), were isolated from the EtOAc-soluble fraction of stem bark of Styrax japonica. Known compounds, taraxerol (6), syringin (7), and (-)-pinoresinol glucoside (8), were also obtained. The structures of styraxligonolides B-F were determined as 2alpha-(4'-hydroxy-3'-methoxyphenyl)-6alpha-(3' ',4' '-methylenedioxyphenyl)-8-oxo-3,7-dioxabicyclo[3.3.0]octane 4'-O-(beta-D-glucopyranoside) (1), (2S,3S)-2alpha-(3' '-hydroxy-4' '-methoxybenzyl)-3beta-(4'-hydroxy-3'-methoxybenzyl)-gamma-butyrolactone 4'-O-(beta-D-glucopyranoside) (2), (2S,3S)-2alpha-(4' '-hydroxy-3' '-methoxybenzyl)-3beta-(4'-hydroxy-3'-methoxybenzyl)-gamma-butyrolactone 4'-O-(beta-D-glucopyranoside) (3), (2S,3S)-2alpha-(4' '-hydroxy-3' '-methoxybenzyl)-3beta-(4'-hydroxy-3'-methoxybenzyl)-gamma-butyrolactone 4' '-O-(beta-D-glucopyranoside) (4), and (2S,3S)-2alpha-(3' ',4' '-dimethoxybenzyl)-3beta-(4'-hydroxy-3'-methoxybenzyl)-gamma-butyrolactone 4'-O-(beta-D-glucopyranoside) (5) by spectroscopic means including 2D NMR. Compounds 1-8 were tested in vitro for antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Styraxlignolide C (2), styraxlignolide D (3), styraxlignolide E (4), and (-)-pinoresinol glucoside (8) exhibited weak radical-scavenging activity in the DPPH assay, with IC50 values of 380, 278, 194, and 260 microM, respectively. New furofuran and butyrolactone lignans with antioxidant activity from the stem bark of Styrax japonica. Min BS, Na MK, Oh SR, Ahn KS, Jeong GS, Li G, Lee SK, Joung H, Lee HK. No
15635221 Phytochemical investigation of Ixeris chinensis NAKAI (Asteraceae) has resulted in the isolation of a new guaianolide-type sesquiterpene lactone, ixerochinolide (1) as well as the related glucoside, ixerochinoside (2). In addition, the known guaianolides, 8beta-hydroxy-3-oxo-guaia-4(15),10(14),11(13)-trien-1alpha,5alpha,6beta,7alphaH-12,6-olide (8beta-hydroxydehydrozaluzanin), 8beta,15-dihydroxy-2-oxo-guaia-1(10),3,11(13)-trien-5alpha,6beta,7alphaH-12,6-olide (lactucin), 3beta,8alpha,10alpha-trihydroxy-guaia-4(15),11(13)-dien-1alpha,5alpha,6beta,7alphaH-12,6-olide (10alpha-hydroxy-10,14-dihydro-desacylcynaropicrin) and 3beta-D-glucopyranosyloxy-8beta-(p-hydroxyphenylacetyloxy)-guaia-4(15),10(14),11(13)-trien-1alpha,5alpha,6beta,7alphaH-12,6-olide (8-epicrepioside) were identified. The structures were determined on the basis of spectral analyses, especially 1- and 2D NMR. Compound 1 exhibited significant cytotoxicity against human PC-3 tumor cells. Two new sesquiterpene lactones from Ixeris chinensis. Khalil AT, Shen YC, Guh JH, Cheng SY. No
15649516 From the stem wood of Erythrina latissima, two isoflavones and a flavanone were isolated and characterized as 7,3'-dihydroxy-4'-methoxy-5'-(gamma,gamma-dimethylallyl)isoflavone (erylatissin A), 7,3'-dihydroxy-6'',6''-dimethyl-4'',5''-dehydropyrano [2'',3'': 4',5']isoflavone (erylatissin B), (-)-7,3'-dihydroxy-4'-methoxy-5'-(gamma,gamma-dimethylallyl)flavanone (erylatissin C), respectively, in addition to 10 known flavonoids. Structures of these compounds were determined on the basis of their spectroscopic data. These compounds showed antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida mycoderma. The isolated compounds also exhibited weak radical scavenging properties towards DPPH radical. Antimicrobial and radical scavenging flavonoids from the stem wood of Erythrina latissima. Chacha M1, Bojase-Moleta G, Majinda RR. No
15656137 OBJECTIVE: To study the chemical constituents from the herb of Gentianopsis paludosa.METHOD: Column chromatogrophy and spectral analysis were used to isolate and identify the constituents.RESULT: Six compounds were isolated and identified as 1,7-dihydroxy-3,8-dimethoxyxanthone (I), 1-hydroxy-3, 7, 8-trimethoxyxanthone (II), 1, 8-dihydroxy-3, 7-dimethoxyxanthone (III), 1-hydroxy-3, 7-dimethoxyxanthone (IV), beta-sitosterol (V), daucosterol (VI).CONCLUSION: Compounds III-VI were isolated from the plant for the first time. [Study on the chemical constituents from the herb of Gentianopsis paludosa]. Wang HD, Tan CY, Du YG, Bai XF, Lu HF. No
15669767 Novel ceramides, rel-(3S,4S,5S)-3-[(2R)-2-hydroxycosanoyl-hexacosanoylamino]-4-hydroxy-5-[(4Z)-tetradecane-4-ene]-2,3,4,5-tetrahydrofuran (1a-g), and a new glucoceramide, 1-O-beta-D-glucopyranosyl-(2S,3S,4R,8E)-2-[(2R)-2-hydroxytetracosanoylamino]-1,3,4-octodecanetriol-8-ene (2) were isolated from the aqueous ethanolic extract of the roots of Incarvillea arguta, together with eight known compounds: beta-sitosterol (3), oleanolic acid (4), ursolic acid (5), piperin (6), maslinic acid (7), beta-sitosterol 6'-O-acyl-beta-D-glucopyranoside (8), 8-epideoxyloganic acid (9), and plantarenaloside (10). Their structures were elucidated on the basis of spectral data including IR, MS, NMR [1H NMR, 13C NMR (distortionless enhancement by polarization transfer), 1H-1H COSY, heteronuclear multiple-quantum coherence, and heteronuclear multiple-bond coherence correlations]. The relative configurations were established by nuclear Overhauser effect spectroscopy experiments and by comparison of the NMR spectral data and coupling constants with those already reported in the literature Novel ceramides and a new glucoceramide from the roots of Incarvillea arguta. Luo Y, Yi J, Li B, Zhang G. No
15678368 The essential oils obtained by hydrodistillation from fresh leaves of Cymbopogon citratus and Ocimum gratissimum growing in Cameroon were analyzed by GC and GC/MS. The main constituents of the oil of Ocimum gratissimum were gamma-terpinene (21.9 %), beta-phellandrene (21.1 %), limonene (11.4 %) and thymol (11.2 %), while the oil of Cymbopogon citratus contained geranial (32.8 %), neral (29.0 %), myrcene (16.2 %) and beta-pinene (10.5 %). The effects of these oils on the growth of Plasmodium berghei were investigated. Both oils showed significant antimalarial activities in the four-day suppressive in vivo test in mice. At concentrations of 200, 300 and 500 mg/kg of mouse per day, the essential oil of C. citratus produced the highest activity with the respective percentages of suppression of parasitaemia: 62.1 %, 81.7 % and 86.6 %. The corresponding values for the oil of O. gratissimum at the same concentrations were 55.0 %, 75.2 % and 77.8 %, respectively. Chloroquine (10 mg/kg of mouse, positive control) had a suppressive activity of 100 %. In vivo antimalarial activity of essential oils from Cymbopogon citratus and Ocimum gratissimum on mice infected with Plasmodium berghei Tchoumbougnang F, Zollo PH, Dagne E, Mekonnen Y. No
15693716 A dichloromethane extract from the leaves of Lithraea molleoides (Anacardiaceae), an argentine medicinal plant, showed cytotoxicity on human hepatocellular carcinoma cell line. Bioassay guided fractionation of this extract led to the isolation of a new active 5-alkyl resorcinol: 1,3-dihydroxy-5-(tridec-4',7'-dienyl)benzene. Chemical structure was established based on spectroscopic data (UV, IR, MS, 1H-NMR, 13C-NMR, COSY). This compound presented cytotoxic activity on 3 human tumoral cell lines: hepatocellular carcinoma cell line-Hep G2 (IC50 +/- SD of 68 +/- 2 microM), mucoepidermoid pulmonary carcinoma cell line-H292 (IC50 +/- SD of 63 +/- 5 microM) and mammary gland adenocarcinoma cell line -MCF7 (IC50 +/- SD of 147 +/- 5). 1,3-dihydroxy-5-(tridec-4',7'-dienyl)benzene: a new cytotoxic compound from Lithraea molleoides. López P, Ruffa MJ, Cavallaro L, Campos R, Martino V, Ferraro G. No
15702639 Three new diterpenes, namely jasonin-a (1), jasonin-b (2), and jasonin-c (3) were isolated from the aerial parts of Jasonia montana (Asteraceae). Their structures were elucidated on the basis of spectral data as [(1E)-2-((2S)-1,2,5-trimethylbicyclo[3.2.l]octan-8-yl)vinyl] benzene-3-carboxylic acid (1), [3-((2S, 5S)-1,2, 5-trimethylcycloheptanyl)propyl]benzene-3-carboxylic acid (2), and [(1E)-3-((7R)-1,7-dimethy-4-methylenecycloheptanyl)prop-1-enyl] benzene-3-carboxylic acid (3). In addition, the previously reported 5,7,3'-trihydroxy-3,6,4'-trimethoxy flavone designated as centaureidin (4), was also isolated and characterized from this source. The different extracts of the plant were also screened for hypoglycemic, antidiabetic, and antimicrobial activities, wherein the petroleum ether and ethanolic extracts exhibited hypoglycemic and antidiabetic activity, and the petroleum ether and chloroform extracts showed antimicrobial activity. Three new diterpenes and the biological activity of different extracts of Jasonia montana. Al-Howiriny TA, Al-Rehaily AJ, Polsc JR, Porter JR, Mossa JS, Ahmed B. No
15706847 OBJECTIVE: To study the chemical constituents from the whole plant of Dracocephalum moldavica.METHOD: The compounds were isolated by using column chromatography with RA polystyrene resin, polyamide and silica gel as packing materials, and the structures of the compounds were identified by means of spectral data.RESULT: eight compounds were identified as apigenin(I), luteolin(II), kaempferol(III), isorhamnetin(IV), tilianin(V), agastachoside(VI), acacetin-7-O-(6-O-Malonyl-beta-D-glucopyranoside) (VII) and syringaresinol(VIII).CONCLUSION: Compounds I, II and III were isolated from genus Dracocephalum for the first time and compounds IV, VII and VIII were isolated from Dracocephalum moldavuca for the first time. [Studies on chemical constituents from herb of Dracocephalum moldavica]. Gu HF, Chen RY, Sun YH, Liu F. No
15709380 OBJECTIVE: To investigate the flavonoid constituents of the Rhododendron anthopogonoides systematically.METHOD: The chemical components were isolated by sephadex LH-20, polyamide and silica gel column chromatography. The chemical structures were elucidated on the basis of physic-chemical properties and spectral data.RESULT: Eight compounds were isolated and identified as: Quercetin (I), isorhamnetin (II), hyperoside (III), cacticin (IV), hirsutine (V), kaemferol-3-beta-D-galactoside (VI) myricetin-3-beta-D-Xyloside (VII), (6''-O-(4-Hydroxybenzoyl) hyperoside (VIII).CONCLUSION: Compounds II, IV, V, VI, VII, and VIII were obtained from this plant for the first time. Compounds VII and VIII were separated from the Ericaceae plant for the first time. [Studies on the flavonoid compounds of Rhododendron anthopogonoides]. Dai SJ, Chen RY, Yu DQ. No
15714817 OBJECTIVE: To study the chemical constituents from Eremosparton songoricum.METHOD: The compounds were isolated with silica gel column chromatography and the structures of these compounds were elucidated by means of spectral analysis.RESULT: The seven compounds were identified as: 5,7,4'-trihydroxyflavone (apigenin) (I), 5-hydroxy-7,4'-dimethoxyflavone (II), 5,7-dihydroxy-3',4'-dimethoxyflavone (III), 5,7-dihydroxy-4'-methoxyflavone (acacetin) (IV), 5,7,4'-trihydroxy-3'-methoxyflavone (chrysoeriol) (V), 5,6,3',4'-tetrahydroxy-7-methoxyflavone (pedalitin) (VI) and 5,4'-dihydroxy-7,3'-dimethoxyflavone-4'-O-D-glucoside (flavogadorinin) (VII).CONCLUSION: These constituents were obtained from E. songoricum for the first time. [Studies on the flavonoid constituents in herb of Eremosparton songoricum]. Ding L, Liu GA, He L, Wang HQ. No
15724403 OBJECTIVE:To study the chemical constituents in bark of Larix olgensis var. koreana.METHOD:The compounds were isolated with silica gel column chromatography and their structures were elucidated on the basis of spectral analysis (IR, EI-MS, 1H-NMR, 13C-NMR).RESULT:Eight compounds were isolated and identified as isopimaric acid (I), beta-sitosterol (II), 24R,5alpha-stigmast-3,6-dione (III), larixol (IV), ferulic acid (V), lariciresinol (VI), secroisolariciresinol (VII) and isolariciresinol (VIII).CONCLUSION:All the compounds were isolated from this plant for the first time. [Studies on chemical constituents in bark of Larix olgensis var. koreana]. Yang BH, Zhang WD, Gu ZB, Li TZ, Zhang C, Zhou Y. No
15729626 Five new caged-tetraprenylated xanthones, scortechinones L - P (1-5), together with six known scortechinones (A, B, D, F, I and J) and one known xanthone, 4 '',5 ''-dihydro-1,5-dihydroxy-6 ',6 '-dimethylpyrano(2 ',3 ':6,7)-4 '',4 '',5 ''-trimethylfurano(2 '',3 '':3,4)- xanthone, were isolated from the crude methanol extract of the stem bark of Garcinia scortechinii. The structures were elucidated by analysis of spectroscopic data and comparison of the NMR data with those reported previously. The antibacterial activity of all caged-polyprenylated xanthones, isolated from the latex and stem bark of G. scortechinii, was evaluated. Scortechinone B (6) exhibited significant antibacterial activity against a methicillin-resistant Staphylococcus aureus strain with an MIC value of 2 microg/mL. From the MIC values, some structure-antibacterial activity relationships were established. Antibacterial caged-tetraprenylated xanthones from the stem bark of Garcinia scortechinii. Rukachaisirikul V, Phainuphong P, Sukpondma Y, Phongpaichit S, Taylor WC. No
15752627 A novel oleanene triterpenetetrol was isolated from the chloroform extract of the aerial parts of Leontodon filii. Its structure was shown to be 2beta,3beta,15alpha,21beta-olean-12-ene-2,3,15,21-tetrol by chemical and spectroscopic methods. The fungicidal efficacy of the chloroform and methanol extracts of the plant was also evaluated, a protective effect being found against Plasmopara viticola, Botrytis cinerea, particularly powerful against Pyricularia oryzae. A novel pentacyclic triterpene from Leontodon filii. Tostão Z, Noronha JP, Cabrita EJ, Medeiros J, Justino J, Bermejo J, Rauter AP. No
15770552 Bioassay-guided fractionation of a CHCl (3) extract prepared from the Mexican medicinal plant Hyptis pectinata led to the isolation of four pyrones, pectinolides A-C ( 1-3) and H ( 4). Activity was tracked using cultured KB cells. Multidrug-resistant strains of Staphylococcus aureus were sensitive to pectinolide H ( 4; KB > 20 microg/mL) in the concentration range of 32-64 microg/mL. The absolute stereochemistry of this new compound was established as 5 S-[(4 S-acetyloxy)-(1 S-hydroxy)-2 Z-octenyl]-2(5 H)-furanone on the basis of spectral, chiroptical data and chemical correlation with pectinolide A ( 1). Mosher ester derivatives were used with pectinolide B ( 2) for confirmation of the 3'-( S) absolute stereochemistry on the side chain chiral center of pectinolides A-C. Anti-staphylococcal and cytotoxic compounds from Hyptis pectinata. Fragoso-Serrano M1, Gibbons S, Pereda-Miranda R. No
15787246 Bioactivity-guided fractionation of the methanol extract from the leaves of Santolina insularis led to the isolation of one new xanthone, (E)-3-(6-[(E)-3-hydroxy-3-oxo-1-propenyl]-9-oxo-9H-xanthen-2-yl)-2-propenoic acid, together with six known flavonoids: hispidulin, nepetin, cirsimaritin, rhamnocitrin, luteolin and luteolin 7-O-beta-D-glucopyranoside. The structures were elucidated by means of 1D-, 2D-NMR spectroscopy and mass spectrometry. The topical anti-inflammatory activity of all isolated compounds and extracts was investigated employing the croton oil-induced dermatitis in mouse ear. The most active compound, luteolin, showed an ID50 of 0.3 micromol/cm(2) and prevented ear oedema more effectively than an equimolar dose of indomethacin within 24 h. Topical anti-inflammatory activity of flavonoids and a new xanthone from Santolina insularis. Cottiglia F, Casu L, Bonsignore L, Casu M, Floris C, Sosa S, Altinier G, Della Loggia R. No
15787450 Two new isoflavonoids, 7-hydroxy-6,4'-dimethoxy-isoflavonequinone (1) and 2'-hydroxy-6,4',6' ',4' ''-tetramethoxy-[7-O-7' ']-bisisoflavone (2), and seven other known flavonoids, 3-hydroxy-9-methoxypterocarpan (medicarpin), 3,10-dihydroxy-9-methoxypterocarpan, 3,9-dimethoxypterocarpan (homopterocarpin) (3), 2,3,9-trimethoxypterocarpan (4), 3,4-dihydroxy-9-methoxypterocarpan (vesticarpan) (5), 2',4,4'-trihydroxychalcone (isoliquiritigenin), and 7,4'-dihydroxyflavanone (liquiritigenin) (6), were isolated from the heartwood of Platymiscium floribundum. The structures of compounds 1 and 2 were established by spectroscopic methods. Compounds 3-6 showed cytotoxic activity when evaluated against five human cancer cell lines in vitro. Cytotoxic flavonoids from Platymiscium floribundum. Falcão MJ1, Pouliquem YB, Lima MA, Gramosa NV, Costa-Lotufo LV, Militão GC, Pessoa C, de Moraes MO, Silveira ER. No
15807240 OBJECTIVE: To study the major chemical constituents from the whole herb of Swertia kauitchensis Franch.METHOD: Compounds were separated and purified with silica gel column and microreticular resin, and their structures were elucidated on the basis of spectroscopic evidence (UV, IR, MS, NMR).RESULT: Ten compounds were identified as oleanolic acid (1), beta-sitosterol (2), daucosterol (3), gentiacaulein (4), bellidifolin (5), methylswertianin (6), swertianin (7), 1,7-dihydroxy-3,4,8-trimethoxyxanthone (8), swertianolin (9),1,3,5,8-tetrahydroxyxanthone (10).CONCLUSION: All compounds were isolated from Swertia kauitchensis Franch for the first time. [Study on chemical constituents from Swertia kauitchensis Franch]. Qing Z, Jiachun C, Yanwen L. No
15814260 Thirty-three plants commonly used in West tropical Africa by traditional healers for the treatment of malaria were collected and ethanolic extracts were obtained by decoction. The antiplasmodial activity of extracts was evaluated in vitro against the chloroquine-resistant FcB1 strain of Plasmodium falciparum. Cytotoxicity was determined on the human MRC-5 and the rat L-6 cell lines. Of the 33 plant extracts, eight (24.5%) showed significant antimalarial activity (IC(50) values ranging from 2.3 to 13.7 microg/ml), 14 (42.5%) weak activity (IC(50) values ranging from 15 to 50 microg/ml) and 11 (33%) appeared inactive (IC(50) values >50 microg/ml). Five plants were of particular interest, associating good antiplasmodial activity and weak cytotoxicity. These five included Nauclea latifolia with known antiplasmodial activity and four, Fagara macrophylla, Funtumia elastica, Phyllanthus muellerianus and Rauvolfia vomitoria, for which the description of antiplasmodial activity is entirely novel. In vitro antiplasmodial activity and cytotoxicity of 33 West African plants used for treatment of malaria. Zirihi GN, Mambu L, Guédé-Guina F, Bodo B, Grellier P. No
15837655 OBJECTIVE: To investigate the effects of Alternanthera philoxeroides Griseb extracts against dengue virus in vitro.METHODS: MTT assay and observation of cytopathic effect (CPE) were carried out to determine the cytotoxicity of Alternanthera philoxeroides Griseb on C6/36 cell lines and its effects on dengue virus.RESULTS: None of the 4 kinds of Alternanthera philoxeroides Griseb extracts exhibited obvious cytotoxicity on the cells at different concentrations with the exception of that over 320 microg/ml. The 4 extracts all showed inhibitory effects on dengue virus. Statistical analysis of TD(50) and ED(50) by Probit regression method suggested that extracts from coumarin had the lowest toxicity on the cells (TD(50)=535.91), whereas extracts from petroleum ether showed the strongest inhibitory effects on dengue virus (ED(50)=47.43) among the 4 extracts.CONCLUSION: Alternanthera philoxeroides Griseb possesses antiviral effects on dengue virus in vitro. [Effects of Alternanthera philoxeroides Griseb against dengue virus in vitro]. Jiang WL, Luo XL, Kuang SJ. No
15856416 The natural product electron transport inhibitor surangin B was examined for its ability to inhibit in vitro mycelial growth and spore germination in several species of fungi. As an inhibitor of mycelial growth, surangin B showed strongest activity against Rhizoctonia solani (IC50 = 3.8 microM) and Botrytis cinerea (IC50 = 11.2 microM). Inhibitory effects were less pronounced in Alternaria dauci, Fusarium oxysporum and Penicillium sp. (IC50 values > 30 microM) and absent in Trichoderma harzianum. Surangin B reduced the level of spore germination in Fusarium oxysporum (IC50 = 2.3 microM) and Botrytis cinerea (IC50 = 1.4 microM), although Alternaria dauci was considerably more tolerant of this coumarin (IC50 = 500 microM). Our results indicate that surangin B may have potential as an antifungal agent. Antifungal properties of surangin B, a coumarin from Mammea longifolia. Deng Y, Nicholson RA. No
15856419 Three new xanthones, 2-hydroxy-1,6,7-trimethoxyxanthone (1), 1,4-dimethoxy-2,3-methylenedioxyxanthone (2), and 7-hydroxy-1,2-dimethoxyxanthone (3), together with five known compounds, 2,7-dihydroxy-1-methoxyxanthone (4), 1-methoxy-2,3-methylenedioxyxanthone (5), 7-hydroxy-1-methoxyxanthone (6), euxanthone (1,7-dihydroxyxanthone) (7), and gentitein (1,3,7-trihydroxyxanthone) (8), were isolated from the roots of Polygala caudata. Their structures were established on the basis of spectral evidence. In the antioxidation activity screening in vitro with luminol chemiluminescence methods, compounds 1 - 5 and 7 and 8 showed H2O2 scavenger activity, with a scavenging effect of 58.4 - 94.5% at 10 microg/mL, and 26.0 - 84.7% at 2 microg/mL. Compounds 4 and 8 also exhibited scavenging effects on the reactive oxygen free radicals produced by macrophage respiratory bursts, with a scavenging effect of 71.7% and 63.4% at 10 microg/mL, 41.2% and 47.8% at 2 microg/mL, respectively. In the vasodilatation assay, compounds 4 - 7 exhibited relaxing activity on the contractions evoked by KCl in Wistar rat thoracic aorta rings in a dose-dependent manner. Xanthones from the roots of Polygala caudata and their antioxidation and vasodilatation activities in vitro. Lin LL, Huang F, Chen SB, Yang DJ, Chen SL, Yang JS, Xiao PG. No
15875672 AIM: To study the chemical constituents of Rhododendron ovatum Planch.METHODS: The chemical constituents were isolated and purified by silica gel column chromatography and identified on the basis of their physiochemical and spectral data.RESULTS: Seven compounds were isolated and identified. Their structures were established as 3,5,7-trihydroxylchromone 3-O-beta-D-xylopyranoside (I), taraxerol (II), beta-sitosterol (III), betulinic acid (IV), quercetin (V), quercetin-3-O-alpha-L-rhamnopyranoside (VI), and D-glucose (VII).CONCLUSION: Compound I is a new compound. Compounds II-VII were isolated from this plant for the first time. The chemical constituents of Rhododendron ovatum Planch. Feng ZM, Wang YH, Zhang PC. No
15885815 Phyllanthus urinaria Linnea (Euphorbiaceae) is one of the traditional medicinal plants that are widely applied by oriental people, especially by Chinese and Indian, to ameliorate various kinds of ailments. Many biological activities, including anti-hepatitis B virus, anti-Epstein-Barr virus and anti-retroviral reverse transcriptase, of P. urinaria have been reported, but not against herpes simplex virus (HSV). In this study, the anti-HSV-1 and HSV-2 activities of different solvents extracted from P. urinaria were investigated in vitro by plaque reduction assay. Results showed that acetone, ethanol and methanol extracts of P. urinaria inhibited HSV-2 but not HSV-1 infection. The 50% inhibitory concentration against HSV-2 infection (IC50) of acetone, ethanol and methanol extracts was 4.3 +/- 0.5, 5.0 +/ -0.4 and 4.0 +/- 0.9 mcg/ml, respectively. All three extracts showed no cytotoxic effect against Vero cells at concentrations of 10.0 mcg/ml or below. The time-of-addition study demonstrated that these three extracts were only effective when added during the HSV-2 infection which, therefore, suggested that they disturb the initial stage of HSV-2 infection. Furthermore, they can diminish virus infectivity without significantly affecting incubation time and temperature. Therefore, the acetone, ethanol and methanol extracts of P. urinaria were concluded to likely inhibit HSV-2 infection through disturbing the early stage of virus infection and through diminishing the virus infectivity. Acetone, ethanol and methanol extracts of Phyllanthus urinaria inhibit HSV-2 infection in vitro. Yang CM, Cheng HY, Lin TC, Chiang LC, Lin CC. No
15921420 Twelve new oligosaccharide multi-esters, tricornoses A-L, together with eight known compounds were isolated from the roots of Polygala tricornis. The structures of the new compounds were elucidated on the basis of chemical and spectroscopic evidence. Tricornoses A-L, oligosaccharide multi-esters from the roots of Polygala tricornis. Li J, Jiang Y, Tu PF. No
15921422 Six new secoiridoid glycosides, gentiascabraside A (1), 6beta-hydroxyswertiajaposide A (2), 1-O-beta-d-glucopyranosyl-4-epiamplexine (3), and scabrans G3 (4), G4 (5), and G5 (6), have been isolated from the rhizomes and roots of Gentiana scabra together with a known compound, swertiajaposide A (7). The structures of the new compounds were determined by spectroscopic (NMR, MS) and chemical means. Secoiridoid glycosides from Gentiana scabra. Kikuchi M, Kakuda R, Kikuchi M, Yaoita Y. No
15921423 Four novel triterpenoid saponins, silenorubicosides A-D (1-4), together with four known saponins were isolated from the roots of Silene rubicunda. Their structures were established on the basis of spectroscopic analysis, including extensive 1D and 2D NMR (DQF-COSY, TOCSY, HETCOR, HMBC, and phase-sensitive NOESY) studies and chemical degradation. Silenorubicosides A-D, triterpenoid saponins from Silene rubicunda. Fu H, Koike K, Li W, Nikaido T, Lin W, Guo D. No
15921433 Four new cinnamylphenols, pulchelstyrenes A (1), B (2), C (3), and D (4), together with citrusinol, yukovanol, methyl piperitol, and 4-hydroxy-2,3-dimethoxybenzaldehyde were isolated from the aerial part of Phyllodium pulchellum. The structures of these compounds were determined by spectroscopic methods. Compounds 2, 3, and citrusinol exhibited marginal cytotoxicity against KB cells, and citrusinol also showed mild cytotoxicity against the HepG2 cell line. Cinnamylphenols from Phyllodium pulchellum. Shen CC, Wang ST, Tsai SY, Yang HC, Shieh BJ, Chen CC. No
15925220 Three extracts of Calendulae officinalis flos (Asteraceae): heptane, ethyl acetate and methanol were introduced to a human skin fibroblast (HSF) cells culture and a culture of human breast cancer cells (T47D), cell culture collection ECACC number 85102201. The ethyl acetate but not the heptane and methanol extracts in concentrations above 25 microg/mL, can stimulate cell proliferation and cellular metabolism by increase of mitochondrial dehydrogenase activity. However, concentrations exceeding 75 microg/mL are toxic for cells. The second part of the study concerned elaborating of optimal chromatographic systems for quantitative analysis of these extracts by the use of HPTLC with densitometry. Oleanolic acid, beta-amyrin, beta-amyrin acetate, rutin, narcissin, 3-glucoside of isorhamnetin, quercetin, isoquercitrin, vanillic acid, caffeic acid, chlorogenic acid, protokatechuic acid, p-coumaric acid and syringic acid were all identified. The influence of Calendulae officinalis flos extracts on cell cultures, and the chromatographic analysis of extracts. Matysik G, Wójciak-Kosior M, Paduch R No
15927216 From the stems and twigs of the mangrove plant, Ceriops tagal, seven dolabrane-type diterpenes, namely tagalsins A-G (1-7), and the norditerpene tagalsin H (8) were isolated. Their structures were established on the basis of extensive spectroscopic analysis. Tagalsins A-H, dolabrane-type diterpenes from the mangrove plant, Ceriops tagal. Zhang Y, Deng Z, Gao T, Proksch P, Lin W. No
15934239 The chemical constituents of Salacia hainanensis were studied for the first time. Five compounds were isolated from the roots of the plant by silica-gel column chromatography and reverse phase preparative chromatography. Four of them have been identified as friedelin, beta-sitosterol, ursolic acid and mangiferin. [Studies on chemical constituents of the roots of Salacia hainanensis]. Yuan G, Yi Y. No
15941314 The chemical composition of the essential oil obtained by hydrodistillation from the aerial parts of Hyptis verticillata Jacq. was elucidated by a combination of GC and GC-MS analyses. The oil was dominated by the sesquiterpenoids cadina-4,10(15)-dien-3-one (15.1%) (1) and aromadendr-1(10)-en-9-one (squamulosone) (30.7%) (2). The oil exhibited chemosterilant activities against the cattle tick, Boophilus microplus Canest., and toxic action against adult Cylas formicarius elegantulus Summer, the most destructive pest of sweet potato (Ipomoea species). Biological activity and chemical composition of the essential oil from Jamaican Hyptis verticillata Jacq. Facey PC, Porter RB, Reese PB, Williams LA. No
15974621 From the leaves of Macaranga tanarius, three new constituents, tanarifuranonol (1), tanariflavanone C (2), and tanariflavanone D (3), together with seven known compounds, were isolated and identified. Substances obtained in this investigation were evaluated against a panel of bioassays. Constituents of the leaves of Macaranga tanarius. Phommart S, Sutthivaiyakit P, Chimnoi N, Ruchirawat S, Sutthivaiyakit S. No
15981875 OBJECTIVE: To study the chemical constituents from Elsholtza bodinieri Vaniot.METHOD: The chemical constituents were isolated and repeatedly purified on silica gel column and the structures were elucidated by the NMR spectra and physico-chemical properties.RESULTS: Seven compounds were obtained and they are identified as 5,7-dihydroxyflavone, 5,7,3',4-tetrahydroxyflavone, 5,3',4'-trihydroxy-7-O-beta-D-glucoside, acacetin, oleanolic acid, beta-sitosterol, daucosterol.CONCLUSION: All the compounds were obtained from Elsholtzia bodinieri Vaniot for the first time. [Studies on chemical constituents from Elsholtzia bodinieri Vaniot]. Zhao D, Yang Y, Zhang W, Liu X, Zhai C, Wang H. No
15981878 OBJECTIVE: To study the chemical constituents from Salvia roborowskii Maxim.METHODS: Chromatography and spectroscopic analysis were employed to isolate and elucidate the chemical constituents in the plant.RESULTS: Three compounds were isolated and elucidated as 1 beta-hydroxy-lupeol (I), quercetin-3-O-beta-D-glucoside (II), rutin (III).CONCLUSION: I - III were isolated from this plant for the first time. [Studies on chemical constituents from Salvia roborowskii Maxim]. Li C, Liu Y, Gao Y, Zhang C. No
15987199 Two novel bisdolabrane backbone tetraditerpenoids, tagalsins I (1) and J (2), were isolated from the mangrove plant, Ceriops tagal, and their structures were elucidated by means of extensive two-dimensional NMR (COSY, HMQC, HMBC, and NOESY), IR, and MS data analysis. The stereochemistry of 1 was further determined by single-crystal X-ray diffraction. Tagalsins I and J, two novel tetraterpenoids from the mangrove plant, Ceriops tagal. Zhang Y, Lu Y, Mao L, Proksch P, Lin W. No
15997139 Chemical investigation of leaves of Staphylea bumalda DC., collected in the suburbs of Hiroshima City, afforded 11 new megastigmane glucosides, named staphylionosides A-K (3-13), along with two known megastigmane glucosides (1, 2). The relative structures were elucidated from spectroscopic evidence, and the absolute structures of the aglycones were determined by means of the combination of beta-D-glucosylation-induced shift-trends and the modified Mosher's method. Staphylionosides A-K: megastigmane glucosides from the leaves of Staphylea bumalda DC. Yu Q1, Matsunami K, Otsuka H, Takeda Y. No
15997152 A new caged-tetraprenylated xanthone, hanburinone (1), was isolated from the fresh fruits of Garcinia hanburyi together with four known caged-tetraprenylated xanthones; isomoreollin B (2), morellin (3), moreollic acid (4) and morellic acid (5). Their structures were elucidated by analysis of spectroscopic data and comparison of the NMR data with those reported previously. Compounds 4 and 5 showed moderately antibacterial activity against methicillin-resistant Staphylococcus aureus with a MIC value of 25 microg/ml. Antibacterial caged-tetraprenylated xanthones from the fruits of Garcinia hanburyi. Sukpondma Y, Rukachaisirikul V, Phongpaichit S. No
16011096 OBJECTIVE: To investigate the flavonoid constituents of Ipomoea batatas.METHOD: The leaf was extracted with EtOH and partitioned with CHCl3, EtOAc and n-BuOH respectively. The chemical components of EtOAc fraction were isolated by silica gel and sephadex LH-20 column chromatography. The chemical structures were elucidated on the basis of physic-chemical properties and spectral data.RESULT: Five compounds were isolated and identified as: tiliroside (I), astragalin (II), rhamnocitrin (III), rhamnetin (IV) and kaempferol (V).CONCLUSION: All of these five compounds were obtained from this plant for the first time. [Study on flavonoids from leaf of Ipomoea batatas]. Luo JG, Kong LY. No
16011283 Abstarct:"OBJECTIVE:To analyse the chemical constituents of Artemisia dracunculus.METHOD:The essential oil from A. dracunculus was extracted by steam distillation and the chemical constituents were analysed by capillary GC-MS method. The relative content of each component was calculated by area normalization.RESULT:36 peaks were separated and all of them were identified. The main chemical components were 3,7-dimethyl-1,3,7-octatriene (38.43%), 1S-alpha-pinene (36.96%), 1-methoxy-4-(2-Propenyl)-benzene (8.57%), limonene (6.33%), 1R-alpha-pinene (3.40%), etc.CONCLUSION:The study provided solid and scientific proof for the further exploitation and utilization of A. dracunculus." [Studies on the chemical constituents of the essential oil of Artemisia dracunculus]. Zhang Y, Zhang J, Yao J, Yang YL, Wang L, Dong LN. No
16038540 The fruits of Garcinia scortechinii afforded 10 new compounds: four caged-tetraprenylated xanthones (scortechinones Q-T, 1-4), four rearranged xanthones (scortechinones U-X, 5-8), and two sesquiterpene derivatives (scortechterpenes A, B, 9, 10), together with 14 known compounds: one sesquiterpene, two biflavonoids, and 11 caged-polyprenylated xanthones. Their structures were elucidated by analysis of spectroscopic data and comparison of the NMR data with those reported previously. All xanthone derivatives were evaluated for antibacterial activity against methicillin-resistant Staphylococcus aureus. Xanthone and sesquiterpene derivatives from the fruits of Garcinia scortechinii. Sukpondma Y, Rukachaisirikul V, Phongpaichit S. No
16038552 Three new C20-diterpenoid alkaloids, trifoliolasines D-F (1-3), were isolated from the aerial parts of Delphinium trifoliolatum, and their structures were determined by the interpretation of spectroscopic data and by the single-crystal X-ray crystallographic analysis of 1. C20-diterpenoid alkaloids from Delphinium trifoliolatum. Zhou XL, Chen DL, Chen QH, Wang FP. No
16075715 OBJECTIVE: To study the chemical constituents of Anoectochilus roxburghii.METHOD: Silica gel and sephadex LH-20 column chromotography were used in the isolation from the ethanol extracts of the whole plant, the compounds were determined on the basis of various modern spectroscopic analysis and physical constants.RESULT: Five compounds were isolated from the CHCl3 soluble portion, identified as p-Hydroxybenzaldehyde (I), ferulic acid (II), quercetin (III), daucosterol (IV), cirsilineol (V).CONCLUSION: All these compounds were isolated from the plant for the first time, compound V was isolated from the Orchid Family for the first time, the other compounds were isolated from this genus for the first time. [Study on chemical constituents in herbs of Anoectochilus roxburghii II]. He CN, Wang CL, Guo SX, Yang JS, Xiao PG. No
16075732 OBJECTIVE: To study the chemical constituents of Dracocephalum moldavica.METHOD: The compounds were isolated by using RA polystyrene resin, polyamide and silica gel column chromatography, The structures of the compounds were elucidated on the basis of physic-chemical properties and spectra data.RESULT: Six compounds were identified as syringaresinol4-O-beta-D-monoglucoside (I), sy-ringaresinol-4,4'-O-bis-beta-D-glucoside (II), kaempferol-3-O-beta-D-(6"-O-p-coumaroyl)-galactopyranoside (III), 2"-p-coumarylastragalin (IV), takakin-8-O-beta-D-glucopyranoside (V), beta-daucosterol (VI).CONCLUSION: Compounds I-V were obtained from genus Dracocephalum for the first time. [Studies on chemical constituents in herbs of Dracocephalum moldavica II]. Gu HF, Chen RY, Sun YH, Xing JG. No
16083924 Two xanthones, bangangxanthone A (1) [1,5,8-trihydroxy-6'-methyl-6'-(4-methylpent-3-enyl)- pyrano[2',3':3,4]xanthone] and B (2) [1,4,8-trihydroxy-2-prenylxanthone], along with two known xanthones, 1,5-dihydroxyxanthone, 2-hydroxy-1,7-dimethoxyxanthone and the pentacyclic triterpenoids, friedelin, oleanolic acid and lupeol were isolated from the chloroform extract of the stem bark of Garcinia polyantha. The structures of these compounds were assigned by spectroscopic analysis. Compounds 1-4 showed antioxidant DPPH radical scavenging activities. Bangangxanthone A and B, two xanthones from the stem bark of Garcinia polyantha Oliv. Lannang AM, Komguem J, Ngninzeko FN, Tangmouo JG, Lontsi D, Ajaz A, Choudhary MI, Ranjit R, Devkota KP, Sondengam BL No
16087640 From the roots of Securidaca inappendiculata, one new sterol glycoside securisteroside (1) has been isolated, along with two known sterols, spinasterol (2) and 3-O-beta-D-glucopyranosyl-spinasterol (3). The new sterol was characterized by chemical and spectrometric methods, including EIMS, FABMS and one- and two-dimensional NMR experiments. A new sterol glycoside from Securidaca inappendiculata. Zhang LJ, Yang XD, Xu LZ, Zou ZM, Yang SL. No
16104502 OBJECTIVE: To investigate the chemical constituents from the flowers of Carthamus tinctorius.METHOD: The chemical constituents were isolated and repeatedly purified on silica gel column. They were identified and structurally elucidated by means of physio-chemical constants and spectral analysis.RESULT: Eight compounds were identifed as palmitic acid (I), 1-O-hexadecanolenin (II), trans-3-tridecene-5,7,9,11-tetrayne-1,2-diol (III), trans-trans-3,11-tridecadiene -5,7,9-triyne -1,2-diol (IV), coumaric acid (V), daucosterol (VI), apigenin (VIl) and kaempferol (VIII), respectively.CONCLUSION: Two compounds (II and III) were obtained from the genus of Carthamus for the first time. [Studies on chemical constituents from the flowers of Carthamus tinctorius L]. Liu Y, Yang J, Liu Q. No
16110862 OBJECTIVE: To study the chemical constituents in roots of P. fallax and their anti-oxidation activities in vitro.METHOD: Column chromatographic techniques were employed for isolation and purification of chemical constituents of the plant. The structures were elucidated on the basis of the spectral evidence and the physical and chemical character. The isolated compounds were screened with four anti-oxidation models in vitro.RESULT: Seven xanthones, 1,7-dihydroxy-2,3-methylenedioxyxanthone (1), 1-methoxy-2,3-methylenedioxyxanthone (2), 3-hydroxy-1,2-dimethoxyxanthone (3), 1,6,7-trihydroxy-2,3-dimethoxyxanthone (4), 7-hydroxy-1-methoxy-2,3-methylenedioxyxanthone (5), 1,3-dihydroxy-2-methoxyxanthone (6) and 1,3,7-trihydroxy-2-methoxyxanthone (7), were isolated from the roots of P. fallax. And compounds 1 - 7 showed different anti-oxidation activities in the different pharmacological models.CONCLUSION: Compounds 2, 3, 5 and 7 were isolated from this plant for the first time. Xanthones from this plant showed anti-oxidation activities. The pharmacological activities of the pure compounds from this plant were also reported for the first time. [Chemical constituents in roots of Polygala fallax and their anti-oxidation activities in vitro]. Lin LL, Huang F, Chen SB, Yang DJ, Chen SL, Yang JS, Xiao PG. No
16114086 Crude extracts and VLC fractions from the stem bark of Barringtonia acutangula (L.) Gaertn (Fam. Lecythidaceae) were screened for their antimicrobial activities against two Gram-positive bacteria, two Gram-negative bacteria and two fungi using a microdilution titre assay. Among the crude extracts, petroleum ether extract showed good activity against all test organisms. The VLC fraction PE 16 was found to be very effective against Bacillus subtilis (MIC=25 microg/ml) and Aspergillus niger (MIC=12.5 microg/ml). The activities were compared to standard antibiotics-kanamycin and fluconazole. The major compound from PE16 was identified as 12, 20(29)-lupadien-3-ol by NMR spectroscopy. Antimicrobial activities of Barringtonia acutangula. Rahman MM, Polfreman D, MacGeachan J, Gray AI No
16115104 Summary In this study, 36 extracts derived from 10 plant species were selected to screen for their antifungal activity against clinical isolates of Candida albicans, Cryptococcus neoformans and Microsporum gypseum. Selection was based on their use by traditional Thai healers or their reported antimicrobial activities in an attempt to find bioactive medicines for use in the treatment of opportunistic fungal infections in AIDS patients. The disc diffusion and hyphal extension-inhibition assays were primarily used to test for inhibition of growth. Minimum inhibitory concentration was determined by dilution methods. The chloroform extracts of Alpinia galanga and Boesenbergia pandurata had pronounced antifungal activity against C. neoformans and M. gypseum, but exhibited weak activity against C. albicans. Alpinia galanga and B. pandurata are excellent candidates for the development of a remedy for opportunistic fungal infections in AIDS patients. Antifungal activities of extracts from Thai medicinal plants against opportunistic fungal pathogens associated with AIDS patients. Phongpaichit S, Subhadhirasakul S, Wattanapiromsakul C. No
16124756 Six new lignans (2-7) were isolated from the bark of Larix olgensis var. koreana, and their structures were determined on the basis of their spectroscopic data. Seven known lignans were also obtained and identified as (+)-lariciresinol 9'-p-coumarate (1), (+)-lariciresinol, (-)-secoisolariciresinol, (+)-isolariciresinol, vladinol D, sesquipinsapol B, and ehletianol C. Compound 1 showed weak inhibition against K562, SHG44, HCT-8, A549, and PC-3M tumor cells with IC50 values of 2.9, 21.4, 32.9, 33.8, and 28.0 microg/mL, respectively. Lignans from bark of Larix olgensis var. koreana. Yang BH, Zhang WD, Liu RH, Li TZ, Zhang C, Zhou Y, Su J. No
16124757 Twelve new compounds, vittarin-A (1), -B (2), -C (3), -D (4), -E (5), -F (6), 3-O-acetylniduloic acid (7), ethyl 3-O-acetylniduloate (8), methyl 4-O-coumaroylquinate (9), vittarilide-A (10), and -B (11), and vittariflavone (12), as well as 20 known compounds have been isolated from the whole plant of Vittaria anguste-elongata. The structures of these compounds were determined by spectroscopic and chemical transformation methods. 5,7-Dihydroxy-3',4',5'-trimethoxyflavone (18) displayed moderate cytotoxicity against human lung carcinoma and central nervous system carcinoma cell lines with inhibition of 89 and 61% at a concentration of 58 microM, respectively. Vittarilide-A (10) and -B (11) and ethyl 4-O-caffeoylquinate (14) exhibited moderate DPPH radical scavenging activity with IC50 values of 91, 290, and 234 microM, respectively. Constituents of Vittaria anguste-elongata and their biological activities. Wu PL, Hsu YL, Zao CW, Damu AG, Wu TS. No
16124764 One new benzopyran, nigrolineabenzopyran A (1), two new biphenyls, nigrolineabiphenyls A and B (2, 3), and four new tetraoxygenated xanthones, nigrolineaxanthones T-W (4-7), were isolated from the crude methanol extract of the twigs of Garcinia nigrolineata along with 11 known xanthones. The xanthones isolated from the twigs as well as those from the stem bark were evaluated for antibacterial activity against methicillin-resistant Staphylococcus aureus. Nigrolineaxanthone F, latisxanthone D, and brasilixanthone showed significant activity, with an equal MIC value of 2 microg/mL. Benzopyran, biphenyl, and tetraoxygenated xanthone derivatives from the twigs of Garcinia nigrolineata. Rukachaisirikul V, Tadpetch K, Watthanaphanit A, Saengsanae N, Phongpaichit S. No
16144318 AIM: To study the chemical constituents of the underground part of Ligularia pleurocaulis (Franch.) Hand-Mazz.METHODS: The dried roots and rhizomes of L. pleurocaulis were extracted with methanol. Isolation and purification were performed by silica gel column chromatography and recrystallization etc. Structures of the pure compounds were established on basis of spectral analysis.RESULTS: Twelve compounds were obtained from L. pleurocaulis, they were 6-angeloyloxy-furanoligularenone (1), 2-oxo-3-hydroxy-eremophila-1(10),3,7(11),8-tetraen-8,12-olide (2), tiglic acid (3), oleanolic acid (4), lupeol (5), beta-sitosterol (6), daucosterol (7), caffeic acid (8), emodin (9), 7-methoxy-coumarin (10), ferulic acid (11) and 4-hydroxy-2,5-dimethoxy-benzaldehyde (12).CONCLUSION: Compound 1 is a new eremophilane and compound 2 is a new natural compound. All above compounds were obtained for the first time from L. pleurocaulis. The chemical constituents of Ligularia pleurocaulis. Zhang M, Zhang CF, Wang ZT. No
16144319 AIM: To study the chemical constituents of Sphaerophysa salsula (Pall.) DC.METHODS: Compounds were isolated by repeated chromatography, on silica gel and Sephadex LH-20 columns and identified by spectroscopic data (ESI-MS, 1H and 13C NMR, HSQC, HMBC).RESULTS: Eight compounds have been isolated and identified as: isorhamnetin-3-O-[6"-(3-hydroxy-3-methylglutaroyl)-beta-D-glucopyranoside] (I), quercitrin (II), n-butyl-O-beta-D-fructopyranoside (III), nicotinic acid (IV), succinic acid (V), erythrol (VI), D-mannitol (VII), uridine (VIII).CONCLUSION: Compound I is a new compound. Compounds I -VI were isolated from this genus for the first time. [A new flavonoid glycoside from the roots and stems of Sphaerophysa salsula]. Hou BL, Li ZL, Li X. No
16144320 AIM: To study the benzophenones in the roots of Securidaca inappendiculata Hassk.METHODS: Column chromatography (including silica gel and Sephadex LH-20) was used to isolate benzophenones whose structures were elucidated by HREI-MS, NMR (1D and 2D) methods.RESULTS: A new benzophenone was isolated and identified as 2-methoxy-3,4-methylenedioxy-benzophenone (I), along with a known compound 4-hydroxy-2,6-dimethoxy-benzophenone (II).CONCLUSION: Compound I is a new one named as securiphenone B, compound II was isolated from the genus for the first time. [A new benzophenone from the root of Securidaca inappendiculata]. Zhang LJ, Zhang L, Xu LZ, Yang XD, Zou ZM, Yang SL. No
16153670 The dichloromethane extract of the stem bark of Warburgia ugandensis afforded three new coloratane sesquiterpenes, namely: 6alpha,9alpha-dihydroxy-4(13),7-coloratadien-11,12-dial (1), 4(13),7-coloratadien-12,11-olide (2), and 7beta-hydroxy-4(13),8-coloratadien-11,12-olide (3), together with nine known sesquiterpenes, i.e., cinnamolide-3beta-acetate (4), muzigadial (5), muzigadiolide (6), 11alpha-hydroxymuzigadiolide (7), cinnamolide (8), 7alpha-hydroxy-8-drimen-11,12-olide (9), ugandensolide (10), mukaadial (11), ugandensidial (12), and linoleic acid (13). Their structures were assigned on the basis of 1D and 2D-NMR spectroscopic and GC-MS analysis. The compounds were examined for their antimycobacterial activity against Mycobacterium aurum, M. fortuitum, M. phlei and M. smegmatis; and the active constituents showed MIC values ranged from 4 to 128 microg/ml compared to the antibiotic drugs ethambutol (MIC ranged from 0.5 to 8 microg/ml) and isoniazid (MIC ranged from 1 to 4 microg/ml). Sesquiterpenes from Warburgia ugandensis and their antimycobacterial activity. Wube AA1, Bucar F, Gibbons S, Asres K. No
16173114 A new polyisoprenylated benzophenone, guttiferone I, together with the known compounds cambogin, 1,7-dihydroxyxanthone, 1,3,6,7-tetrahydroxyxanthone and 1,3,5,6-tetrahydroxyxanthone were isolated from the stem bark of Garcinia griffithii. The acetone extract of the heartwood of Garcinia mangostana contained one new diprenylated xanthone (mangoxanthone) and a new benzophenone (3',6-dihydroxy-2,4,4'-trimethoxybenzophenone) as well as the known xanthones dulxanthone D, 1,3,7-trihydroxy-2-methoxyxanthone, 1,3,5-trihydroxy-13,13-dimethyl-2H-pyran[7,6-b]xanthen-9-one. Their structures were established on the basis of spectroscopic studies and chemical correlation. Xanthones and benzophenones from Garcinia griffithii and Garcinia mangostana. Nguyen LH, Venkatraman G, Sim KY, Harrison LJ. No
16243465 Sesuvium portulacastrum has long been used as a remedy for fever and scurvy. Hydrodistillation was used to extract the essential oil from the fresh leaves of Sesuvium portulacastrum. The essential oil yield obtained was 0.15%. Using GC-MS analysis, alpha-pinene, camphene, beta-pinene, alpha-terpinene, O-cymene, limonene, 1,8-cineole, alpha-terpinene, bornyl acetate, tridecane, trans-caryophyllene and alpha-humulene were identified. The hole plate diffusion method was used for antibacterial testing. The essential oil exhibited antibacterial activity against Acetobacter calcoacetica, Bacillus subtillis, Clostridium sporogenes, Clostridium perfringens, Escherichia coli, Salmonella typhii, Staphylococcus aureus and Yersinia enterocolitica. The mycelium growth inhibition method was used for the antifungal testing. The oil exhibited antifungal activity against Candida albicans, Aspergillus niger, Aspergillus flavus and Penicillium notatum. Using the beta-carotene, acetone and linoleic acid method for the antioxidant testing, the essential oil showed antioxidant activity threshold of 15.9 mm mean zone of color retention. Chemical composition and biological activities of essential oil from the leaves of Sesuvium portulacastrum. Magwa ML, Gundidza M, Gweru N and Humphery G No
16297892 Two new apigenin triglycosides, apigenin 6-C-[beta-D-xylopyranosyl-(1'''-->2'')-beta-D-galactopyranoside]-7-O-beta-D-glucopyranoside and apigenin 6-C-[beta-D-xylopyranosyl-(1'''-->2'')-beta-D-galactopyranoside]-7-O-beta-D-(6-O-p-coumarylglucopyranoside) were isolated from the ethanol extract of the seeds of Syzygium aromaticum. Their structures were elucidated by chemical and spectral analysis (UV, FABMS, 1H, 13C NMR, HMQC, HMBC, NOESY and DEPT). Flavonoid triglycosides from the seeds of Syzygium aromaticum. Nassar MI. No
16297941 The CH(2)Cl(2)/MeOH extract of the stem bark of Erythrina vogelii (Fabaceae) from Nigeria has yielded two novel isoflavones, 7,4'-dihydroxy-8-(gamma,gamma-dimethylallyl)-2''zeta-(4''-hydroxyisopropyl)dihydrofurano[1'',3'':5,6]isoflavone (vogelin H) (1) and 7,4'-dihydroxy-8-[(2'''zeta,3'''-dihydroxy-3'''-methyl)butyl]-2'',2''-dimethyl-3'',4''-dehydropyrano[1'',4'':5,6]isoflavone (vogelin I) (2), a novel flavone, 7,4'-dihydroxy-2'',2''-dimethyl-3'',4''-dehydropyrano[1'',4'':5,6]flavone (vogelin J) (3), and eight known flavonoids. Flavones and isoflavones from the west African Fabaceae Erythrina vogelii. Kamdem Waffo AF1, Coombes PH, Mulholland DA, Nkengfack AE, Fomum ZT. No
16308195 Two new bisbenzylisoquinoline alkaloids, neothalfine (1) and thaliatrine (2), together with three known dimeric alkaloids, thalifaberine, thalistine, and thalirecebine, have been isolated from the roots of Thalictrum atriplex Finet et Gagnep. Their structures have been established by spectroscopy. Compound 1 showed in vitro antiplatelet aggregation activities. Novel dimeric alkaloids from the roots of Thalictrum atriplex. Gao GY1, Chen SB, Chen SL, Wang LW, Xiao PG. No
16310231 Two xanthones and two caged-prenylated xanthones, named cochinchinones A-D, respectively, and a synthetically known caged-prenylated xanthone, together with seven known compounds were isolated from the roots of Cratoxylum cochinchinense (Lour.) Blume. Their structures were assigned on the basis of analyses of spectroscopic data. Some of the compounds exhibited effective antioxidative properties. Xanthone derivatives from Cratoxylum cochinchinense roots. Mahabusarakam W, Nuangnaowarat W, Taylor WC. No
16323543 OBJECTIVE: To study the chemical constituents of the flowers of Apocynum venetum.METHOD: Chromatographic methods were used to isolate compounds from the flowers of A. venetum and spectral methods were used to identify the structures of the isolated compounds.RESULT: Seven compounds, kaempferol (I), quercetin (II), quercetin-3-O-beta-D-glucoside (III), kaempferol-3-O-beta-D-glucoside (IV), vanillic acid (V), baimaside (VI), daucosterol (III), were isolated from the flowers of A. venetum.CONCLUSION: Compound I, V, VI, VII were obtained from this plant for the first time. [Studies on chemical constituents from flowers of Apocynum venetum]. Chen L, Du Li-jun, Ding Y, Xing DM, Wang W. No
16335823 OBJECTIVE: To in vestigate the constituents in root of Gentiana macrophylla. METHOD: Various column chromatographic techniques were used for isolation and purification of the principles. The structures were elucidated on the basis of spectral data (UV, IR, MS, 1H-, 13C-NMR etc.) and identified by comparing with standard substance. RESULT: Eight compounds were identified. Four compounds isolated from the chloroform fraction are: 5-carboxyl-3,4-dihydrogen-1H-2-benzopyran-1-one (1), erythrocentauric acid (2), roburic acid (3), oleanolic acid (4). Water fraction gave four known secoiridoid glucosides. They were: gentiopicroside (5), swertiamarine (6), sweroside (7), 6'-O-beta-D-glucosylgentiopicroside (8). CONCLUSION: 1 is a novel compound. It was named as erythrocentauric acid. 2 was isolated from genus Gentiana and 8 was isolated from G. macrophylla for the first time. [Studies on the chemical constituents in root of Gentiana macrophylla from Shaanxi]. Chen QL, Shi ZY, Tu GZ, Sun WJ. No
16364643 (+)-12alpha-Hydroxysophocarpine (8), a new quinolizidine alkaloid was isolated from the roots of Sophora flavescens, together with 10 known quinolizidine alkaloids, (+)-oxymatrine (1), (+)-matrine (2), (+)-9alpha-hydroxymatrine (3), (+)-allomatrine (4), (+)-oxysophocarpine (5), (-)-sophocarpine (6), (-)-9alpha-hydroxysophocarpine (7), (+)-lehmannine (9), (-)-13,14-dehydrosophoridine (10), and (-)-anagyrine (11). Their structures were elucidated by spectroscopic methods, and the stereochemistry of 8 was confirmed by X-ray analysis. These alkaloids were tested for anti-hepatitis B virus (HBV) activity in vitro, compounds 5, 6, 9, and 10 showed significant anti-HBV activity with inhibitory potency against HBsAg secretion at 48.3-79.3% and that against HBeAg secretion at 24.6-34.6%. (+)-12alpha-Hydroxysophocarpine, a new quinolizidine alkaloid and related anti-HBV alkaloids from Sophora flavescens. Ding PL, Liao ZX, Huang H, Zhou P, Chen DF. No
16375831 Juniper essential oil (Juniperi aetheroleum) was obtained from the juniper berry, and the GC/MS analysis showed that the main compounds in the oil were alpha-pinene (29.17%) and beta-pinene (17.84%), sabinene (13.55%), limonene (5.52%), and mircene (0.33%). Juniper essential oil was evaluated for the antimicrobial activity against sixteen bacterial species, seven yeast-like fungi, three yeast and four dermatophyte strains. Juniper essential oil showed similar bactericidal activities against Gram-positive and Gram-negative bacterial species, with MIC values between 8 and 70% (V/V), as well as a strong fungicidal activity against yeasts, yeast-like fungi and dermatophytes, with MIC values below 10% (V/V). The strongest fungicidal activity was recorded against Candida spp. (MIC from 0.78 to 2%, V/V) and dermatophytes (from 0.39 to 2%, V/V). Antimicrobial activity of juniper berry essential oil (Juniperus communis L., Cupressaceae). Pepeljnjak S, Kosalec I, Kalodera Z, Blazević N. No
16378382 A new benzophenone O-glycoside, tricornoside A, and five new xanthone O-glycosides, tricornosides B-F, were isolated from the roots of Polygala tricornis together with three known glycosides. The structures of new compounds were elucidated on the basis of chemical and spectroscopic evidence. Xanthone O-glycosides and benzophenone O-glycosides from the roots of Polygala tricornis. Li J, Jiang Y, Tu PF. No
16379418 OBJECTIVE: To study the chemical Constituents in the root of Rheum glabricaule.METHODS: Compounds were isolated by various column chromatographies with sillica gel. Their structures were identified by spectral analysis (MS, 1HNMR, 13 CNMR) and chemical evidences.RESULTS: Seven compounds were isolated from this plant, including n-hexacosnic acid (I), palmitic acid (II), daucosterol (III), chrysophanol-8-Me ether (IV), citreorosein (V), chrysophanol 8-O-beta-D-glucopyranoside (VI) and 2,5-dimethyl-7-methoxychromone (VII).CONCLUSION: All above compounds are obtained from this plant for the first time. Studies on chemical constituents of Rheum glabricaule. Wei Y, Wu X, Zhang C, Li C. No
16395418 The leaf of Psidium guajava Linn. (family, Myrtaceae) is used traditionally in African folk medicine to manage, control, and/or treat a plethora of human ailments, including diabetes mellitus and hypertension. In order to scientifically appraise some of the anecdotal, folkloric, ethnomedical uses of P. guajava Linn., the present study was undertaken to investigate the hypoglycemic and hypotensive effects of P. guajava leaf aqueous extract (PGE, 50-800 mg/kg) in rat experimental paradigms. The hypoglycemic effect of the plant's extract was examined in normal and diabetic rats, using streptozotocin (STZ)-induced diabetes mellitus model. Hypertensive Dahl salt-sensitive rats were used to investigate the hypotensive (antihypertensive) effect of the plant's extract. Chlorpropamide (CPP; 250 mg/kg, p.o.) was used as the reference hypoglycemic agent for comparison. Acute oral administrations of the plant's extract (PGE; 50-800 mg/kg, p.o.) caused dose-related, significant (p < 0.05-0.001) hypoglycemia in normal (normoglycemic) and STZ-treated, diabetic rats. Moreover, acute intravenous administrations of the plant's extract (PGE, 50-800 mg/kg i.v.) produced dose-dependent, significant reductions (p < 0.05-0.001) in systemic arterial blood pressures and heart rates of hypertensive, Dahl salt-sensitive rats. Although the exact mechanisms of action of the plant's extract still remain speculative at present, it is unlikely that the extract causes hypotension in the mammalian experimental animal model used via cholinergic mechanisms, since its cardiodepressant effects are resistant to atropine pretreatment. The numerous tannins, polyphenolic compounds, flavonoids, pentacyclic triterpenoids, guiajaverin, quercetin, and other chemical compounds present in the plant are speculated to account for the observed hypoglycemic and hypotensive effects of the plant's leaf extract. However, the results of this experimental animal study indicate that the leaf aqueous extract of P. guajava possesses hypoglycemic and hypotensive properties, and thus lend pharmacological credence to the suggested folkloric, ethnomedical uses of the plant in the management or control of adult-onset, type 2 diabetes mellitus and hypertension in some rural African communities. Hypoglycemic and hypotensive effects of Psidium guajava Linn. (Myrtaceae) leaf aqueous extract. Ojewole JA. No
16400942 OBJECTIVE: To investigate the alkaloids from the stem of Artabotrys hainanensis.METHOD: Compounds in plant extracts were separated by silica gel and sephadex LH-20 column chromatography. Chemical structures were elucidated by chemical and spectral analyses including UV, 1H-NMR, 13C-NMR, ESIMS and ESI-MS-MS.RESULT: Eight alkaloids were isolated and identified as spinosine (1), 3-hydroxynornuciferine (2), juzirine (3), artabotrine (4), liridine (5), assimilobine (6), isococlaurine (7), N-demethylarmepavine (8).CONCLUSION: All alkaloids were isolated from this plant for the first time and compounds 1, 3, 7 and 8 were isolated from genus Artabotrys for the first time. [Studies on the alkaloids from stem of Artabotrys hainanensis]. Han CR, Zhu GY, Chen GY, Zhang HY, Bi HP, Fang HX. No
16408812 AIM:To study the alkaloid constituents of the root of Lindera angustifolia Cheng.METHODS:The constituents were isolated and purified by column chromatography and the structures were characterized by spectral analysis.RESULTS:Seven aporphine alkaloids, laurotetanine (I), N-methyllaurotetanine (II), boldine (III), isoboldine (IV), norboldine (V), N-ethoxycarbonyllaurotetanine (VII) and a quaternary isoquinoline alkaloid, magnocurarine (VI), were isolated and identified. The structure of VII was further identified by semi-synthesis with I as starting material.CONCLUSION:All compounds were obtained from this plant for the first time and compound VII was found as a naturally occurring compound for the first time. [Alkaloids from the root of Lindera angustifolia]. Zhao QZ1, Zhao YM, Wang KJ. No
16408813 AIM: To investigate the secondary metabolites of the mangrove plant Ceriops tagal.METHODS: Column chromatography techniques including HPLC were used for the separation and purification, and extensive spectral analysis including various 2D NMR spectra were employed for structure elucidation.RESULTS: Nine compounds, namely, tagalsins A (1), ent-5alpha-dolabr-4 (18) -ene-15S,16-diol (2), squalene (3), betulinic acid (4), lup-20 (29) -en-3-on-28-oic acid (5), betulin (6), lup-20 (29) -en-3-on-28-ol (7), beta-sitosterol (8), n-hexacosanylferulate (9) were obtained. Of which 1 and 2 belong to dolabrane diterpene.CONCLUSION: Compound 1 is a new compound, and 2 to 9 are isolated from this species for the first time. [Chemical constituents from the mangrove plant Ceriops tagal]. Zhang Y, Deng ZW, Gao TX, Fu HZ, Lin WH. No
16422538 OBJECTIVE: To investigate the constituents of Elsholtzia blanda.METHOD: The chemical components were isolated by polyamide and silica gel column chromatography. Their structures were identified with extensive spectral (EI-MS, ESI-MS, 1H-NMR, 13C-NMR, DEPT, 1H-1H-COSY, HMBC, HMQC) and chemical methods.RESULT: Five compounds were isolated and identified as luteolin (I), luteolin-5-O-beta-D-glucopyranoside (II), luteolin-7-O-beta-D-glucopyranoside (III), 5-hydroxy-7, 8 -dimethoxyflavone (IV) and 5-hydroxy-6, 7-dimethoxyflavone (V).CONCLUSION: Compounds III, IV, V were isolated from E. blanda for the first time and I was firstly separated from the genus Elsholtzia [Chemical constituents from Elsholtzia blanda]. Chen HY, Zhou CX, Lou YJ, Duan ZH, Zhao Y. No
16441073 Four new Daphniphyllum alkaloids, daphnilongeranins A-D (1-4), along with four known ones, daphniphylline, longistylumphylline A, deoxyisocalyciphylline B, and daphnicyclidin D, were isolated from the leaves and stems of Daphniphyllum longeracemosum. Daphnilongeranin A (1) is the first seco-10,17-longistylumphylline A type Daphniphyllum alkaloid, and daphnilongeranin B (2) is a new C-22 nor-Daphniphyllum alkaloid based on a new C21 skeleton. Their structures and stereochemistry were determined using spectroscopic data and chemical methods Alkaloids from Daphniphyllum longeracemosum. Yang SP, Zhang H, Zhang CR, Cheng HD, Yue JM. No
16445836 Natural products are still an untapped source of promising lead compounds for the generation of antineoplastic drugs. Here, we investigated for the first time the antiproliferative and apoptotic effects of highly purified oxindole alkaloids, namely isopteropodine (A1), pteropodine (A2), isomitraphylline (A3), uncarine F (A4) and mitraphylline (A5) obtained from Uncaria tomentosa, a South American Rubiaceae, on human lymphoblastic leukaemia T cells (CCRF-CEM-C7H2). Four of the five tested alkaloids inhibited proliferation of acute lymphoblastic leukaemia cells. Furthermore, the antiproliferative effect of the most potent alkaloids pteropodine (A2) and uncarine F (A4) correlated with induction of apoptosis. After 48 h, 100 micromol/l A2 or A4 increased apoptotic cells by 57%. CEM-C7H2 sublines with tetracycline-regulated expression of bcl-2, p16ink4A or constitutively expressing the cowpox virus protein crm-A were used for further studies of the apoptosis-inducing properties of these alkaloids. Neither overexpression of bcl-2 or crm-A nor cell-cycle arrest in G0/G1 phase by tetracycline-regulated expression of p16INK4A could prevent alkaloid-induced apoptosis. Our results show the strong apoptotic effects of pteropodine and uncarine F on acute leukaemic lymphoblasts and recommend the alkaloids for further studies in xenograft models. Oxindole alkaloids from Uncaria tomentosa induce apoptosis in proliferating, G0/G1-arrested and bcl-2-expressing acute lymphoblastic leukaemia cells. Bacher N, Tiefenthaler M, Sturm S, Stuppner H, Ausserlechner MJ, Kofler R, Konwalinka G. No
16468372 OBJECTIVE: To study the chemical constituents in root of Coleusforskohlii.METHOD: The chemical constituents were isolated by column chromatography. The structures were elucidated on the basis of IR, MS, 1H-NMR, 13C-NMR and 2D-NMR experiments.RESULT: Six compounds were obtained and the structures were identified as 14-deoxycoleon U (1), demethylcryptojaponol (2), alpha-amyrin (3), betulic acid (4), alpha-cedrol (5) and beta-sitosterol (6).CONCLUSION: Compounds 1 and 2 were isolated from the genus Coleus for the first time. Compounds 3 and 4 were isolated from C. forskohlii for the first time. [Studies on the chemical constituents in root of Coleus forskohlii]. Xu LL, Lu J, Li WJ, Kong LY. No
16483385 BACKGROUND:The antimicrobial activity and Minimal Inhibitory Concentration (MIC) of the extracts of Bidens pilosa L., Bixa orellana L., Cecropia peltata L., Cinchona officinalis L., Gliricidia sepium H.B. & K, Jacaranda mimosifolia D.Don, Justicia secunda Vahl., Piper pulchrum C.DC, P. paniculata L. and Spilanthes americana Hieron were evaluated against five bacteria (Staphylococcus aureus, Streptococcus beta hemolític, Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli), and one yeast (Candida albicans). These plants are used in Colombian folk medicine to treat infections of microbial origin.METHODS:Plants were collected by farmers and traditional healers. The ethanol, hexane and water extracts were obtained by standard methods. The antimicrobial activity was found by using a modified agar well diffusion method. All microorganisms were obtained from the American Type Culture Collection (ATCC). MIC was determined in the plant extracts that showed some efficacy against the tested microorganisms. Gentamycin sulfate (1.0 microg/ml), clindamycin (0.3 microg/ml) and nystatin (1.0 microg/ml) were used as positive controls.RESULTS:The water extracts of Bidens pilosa L., Jacaranda mimosifolia D.Don, and Piper pulchrum C.DC showed a higher activity against Bacillus cereus and Escherichia coli than gentamycin sulfate. Similarly, the ethanol extracts of all species were active against Staphylococcus aureus except for Justicia secunda. Furthermore, Bixa orellana L, Justicia secunda Vahl. and Piper pulchrum C.DC presented the lowest MICs against Escherichia coli (0.8, 0.6 and 0.6 microg/ml, respectively) compared to gentamycin sulfate (0.9 8 g/ml). Likewise, Justicia secunda and Piper pulchrum C.DC showed an analogous MIC against Candida albicans (0.5 and 0.6 microg/ml, respectively) compared to nystatin (0.6 microg/ml). Bixa orellana L, exhibited a better MIC against Bacillus cereus (0.2 microg/ml) than gentamycin sulfate (0.5 microg/ml).CONCLUSION:This in vitro study corroborated the antimicrobial activity of the selected plants used in folkloric medicine. All these plants were effective against three or more of the pathogenic microorganisms. However, they were ineffective against Streptococcus beta hemolytic and Pseudomonas aeruginosa. Their medicinal use in infections associated with these two species is not recommended. This study also showed that Bixa orellana L, Justicia secunda Vahl. and Piper pulchrum C.DC could be potential sources of new antimicrobial agents. Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: a possible alternative in the treatment of non-nosocomial infections. Rojas JJ, Ochoa VJ, Ocampo SA, Muñoz JF. PMC1395329
16494028 OBJECTIVE: To study the chemical constituents from leaves of Phyllostachys pubescens.METHOD: Column chromatography on sephadex LH-20 as well as preparative HPLC and spectral analysis were used to isolate and elucidate the constituents.RESULT: Six compounds were isolated from leaves of Phyllostachys pubescens, and identified as vanillin (I), syringic aldehyde (II), p-hydroxybenzaldehyde (III), vanillic acid (IV), syringic acid (V), 4-hydroxy-3-methoxy cinnamic acid, ethyl ester (VI).CONCLUSION: All above the compounds were isolated from the plant for the first time. [Chemical constituents from leaves of Phyllostachys pubescens I]. Zhou HY, Zhang H, Li SM. No
16499020 OBJECTIVE: To further investigate the flavonoids of Rhododendron anthopogonoide.METHOD: The compounds were isolated and purified by Sephadex LH-20, polyamide and silica gel column chromatography. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.RESULT: Six compounds were isolated and identified as: taxifolin (I), guaijaverin (II), reynoutrin (III), quercitrin (IV), polystachoside (V) and quercetin-4'-O-beta-D-galactoside (VI).CONCLUSION: For the first time, compound VI was separated from Ericaceae plant, compounds II and V were isolated from Rhododendron plant, and compounds I and II were obtained from this plant. [Studies on the flavonoids in stem of Rhododendron anthopogonoide II]. Dai SJ, Yu DQ. No
16499021 OBJECTIVE: To study the chemical constituents of the root of Rhaponticum uniflorum.METHOD: Separation and purification were performed on silica gel and Sephadex LH-20 column chromatography. Their structure were elucidated on the basis of physicochemical and spectral analysis.RESULT: Five triterpenoid compounds were isolated and identified as ursolic acid (1), 3-oxo-19alpha-hydroxyurs-12-en-28-oic acid (2), pomolic acid (3), arjunic acid (4) and tormentic acid (5), respectively.CONCLUSION: Compounds 1 approximately 5 were isolated from the genus Rhaponticum for the first time. [Triterpenes from root of Rhaponticum uniflorum]. Zhang YH, Zhang JG, Xie JM, Chen GL, Cheng DL. No
16499331 A new trinorcycloartane triterpenoid, lancifodilactone H (1), and a new A ring-secocycloartane triterpenoid, lancifoic acid A (2), together with a known compound, nigranoic acid (3), were isolated from the leaves and stems of Schisandra lancifolia. Their structures were elucidated by spectroscopic data analysis. Compound 1 features a biosynthetically modified seven-membered lactone ring, which was confirmed by single-crystal X-ray diffraction. Compounds 1-3 exhibited weak anti-HIV-1 activity in vitro. Triterpenoids from Schisandra lancifolia with anti-HIV-1 activity. Xiao WL, Tian RR, Pu JX, Li X, Wu L, Lu Y, Li SH, Li RT, Zheng YT, Zheng QT, Sun HD. No
16514886 OBJECTIVE: To study the chemical constituents of Buddleja purdomii W. W Smith.METHODS: The constituents were isolated and purified by various chromatographic methods and structurally identified by spectral analysis.RESULTS: 4 compounds were obtained as cryptomeridiol (I), aucubin (II), galactilol (III), daucosterol (IV).CONCLUSION: All these compounds are obtained from this plant for the first time. [Study on the chemical constituents of Buddleja purdomii]. Zhang Y, Li C, Zhang C, Tao B. No
16518004 Burkholderia pseudomallei (Pseudomonas pseudomallei) causes melioidosis, a life-threatening infection common among paddy cultivators in Southeast Asian countries. No plant materials have been investigated for its activity against B. pseudomallei. Therefore, a preliminary study was carried out using disc diffusion and minimum inhibitory concentration (MIC) methods to evaluate the anti-B. pseudomallei activity of five Indian medicinal plants documented to have been used for several ailments in the ancient Indian scriptures. The leaf extracts of Tamarindus indica, Lawsonia inermis, and Hibiscus rosa-sinensis, the rhizome extracts of Curcuma longa and the seeds of Vigna radiata were prepared using methanol as solvent. The disc diffusion and MIC methods were used to assess the anti-B. pseudomallei activity of the plants tested. Only methanol leaf extracts of Tamarindus indica exhibited anti-B. pseudomallei activity starting from disc concentrations of 150 mug by the disc diffusion method. The other plants failed to show any zone of inhibition. MIC assay revealed that the MIC and minimum bactericidal concentration (MBC) for B. pseudomallei were 125 mug/ml. Our preliminary finding showed that methanolic extracts of Tamarindus indica has anti-B. pseudomallei inhibitory potentials under in vitro conditions. Extensive animal studies may be required before investigating the role of Tamarindus indica for treating melioidosis. The effect of methanolic extract of Tamarindus indica Linn. on the growth of clinical isolates of Burkholderia pseudomallei. Muthu SE, Nandakumar S, Rao UA No
16536578 As part of ongoing research on cancer chemopreventive agents from botanical dietary supplements, Garcinia mangostana L. (commonly known as mangosteen) was selected for detailed study. Repeated chromatography of a CH2Cl2-soluble extract of the pericarp led to the isolation of two new highly oxygenated prenylated xanthones, 8-hydroxycudraxanthone G (1) and mangostingone [7-methoxy-2-(3-methyl-2-butenyl)-8-(3-methyl-2-oxo-3-butenyl)-1,3,6-trihydroxyxanthone, 2], together with 12 known xanthones, cudraxanthone G (3), 8-deoxygartanin (4), garcimangosone B (5), garcinone D (6), garcinone E (7), gartanin (8), 1-isomangostin (9), alpha-mangostin (10), gamma-mangostin (11), mangostinone (12), smeathxanthone A (13), and tovophyllin A (14). The structures of compounds 1 and 2 were elucidated by spectroscopic data analysis. Except for compound 2, which was isolated as a minor component, the antioxidant activities of all isolates were determined using authentic and morpholinosydnonimine-derived peroxynitrite methods, and compounds 1, 8, 10, 11, and 13 were the most active. Alpha-mangostin (10) inhibited 7,12-dimethylbenz[alpha]anthracene-induced preneoplastic lesions in a mouse mammary organ culture assay with an IC50 of 1.0 microg/mL (2.44 microM). Antioxidant xanthones from the pericarp of Garcinia mangostana (Mangosteen). Jung HA, Su BN, Keller WJ, Mehta RG, Kinghorn AD. No
16562826 Six new ent-labdane diterpenoids, 3-O-beta-D-glucopyranosyl-14,19-dideoxyandrographolide (1), 14-deoxy-17-hydroxyandrographolide (2), 19-O-[beta-D-apiofuranosyl(1-->2)-beta-D-glucopyranoyl]-3,14-dideoxyandrographolide (3), 3-O-beta-d-glucopyranosylandrographolide (4), 12S-hydroxyandrographolide (5), and andrographatoside (6), together with 17 known analogues, were isolated from the aerial parts of Andrographis paniculata. The structures of 1-6 were determined by spectroscopic data analysis. All compounds isolated were evaluated for their inhibitory activity against several bacterial and fungal strains. ent-Labdane diterpenoids from Andrographis paniculata. Shen YH, Li RT, Xiao WL, Xu G, Lin ZW, Zhao QS, Sun HD. No
16568659 To do investigation of flavones from the leaves of Cyclocarya paliums (Batal.) Iljinsk. The pure products were isolated by column chromatography of silica gel, thin layer chromatography of silica gel and column chromatography of Sephadex LH-20, their chemical structures were elucidated by NMR, IR and UV spectroscopic evidence. Four flavones were isolated from the leaves of Cyclocarya paliums. They are quercetin (I), kaempferol (II), kaempferol-7-O-alpha-L-rhamnopyranosyl (III) ,and kaempferol-4'-methoxyl-7-O-beta-D-mancopyranosyl (IV). Compounds III and IV are obtained for the first time from this plants. [Studies on flavones in the leaves of Cyclocarya paliums (batal.) Iljinsk]. Li J, Lu Y, Xu Z, Li F, Wen G, Su X. No
16568660 The ethanol extract from Akebia quinata was seperated by dichloromethane, n-butanol in sequence. Six compounds were isolated and identified as beta-sitosterol (I), delta(5,22) stigmasterol (II), oleanolic acid (III), hederagenin (IV), daucosterol (V), delta(5,22) stigmasterol-3-O-beta-D-glycopyranoside (VI). Compound VI was isolated from Akebia plants for the first time. [Study on sterols and triterpenes from the stems of Akebia quinata]. Liu G, Zheng J, Yu Z, Zhang J, Lin R. No
16570801 OBJECTIVE: To investigate the chemical constituents from the root of Lasianthus acuminatissimus of Rubiaceae.METHOD: Normal, reverse phase silica gel, macroporous resin column and HPLC chromatography were used for isolation. Spectroscopic methods (13C-NMR, 1H-NMR, DEPT, EI-MS) were used for identification.RESULT: Six compounds were isolated and elucidated as isoscopletin(I), isoscopletin-6-O-beta-D-glucopyranoside (II), beta configuration of Mudanoside-A (Ill), asperuloside (IV), (-)-pinoresinol 4-O-beta-D-glucopyranoside (V), daucosterol (VI).CONCLUSION: These compounds were obtained from this plant for the first time. [Chemical constituents from root of Lasianthus acuminatissimus I]. Li B, Zhang DM, Luo YM. No
16571139 Abstarct:"BACKGROUND:Candida albicans has become resistant to the already limited, toxic and expensive anti-Candida agents available in the market. These factors necessitate the search for new anti-fungal agents.METHODS:Sixty-three plant extracts, from 56 Tanzanian plant species obtained through the literature and interviews with traditional healers, were evaluated for anti-Candida activity. Aqueous methanolic extracts were screened for anti-Candida activity by bioautography agar overlay method, using a standard strain of Candida albicans (ATCC 90028).RESULTS:Twenty- seven (48%) out of the 56 plants were found to be active. Extracts of the root barks of Albizia anthelmintica and Balanites aegyptiaca, and roots of Plectranthus barbatus showed strong activity.CONCLUSION:The extracts that showed strong anti-Candida activity are worth of further investigation in order to isolate and identify the active compounds." Screening of Tanzanian medicinal plants for anti-Candida activity. Runyoro DK, Matee MI, Ngassapa OD, Joseph CC, Mbwambo ZH. PMC1481531
16595969 Bioassay-directed fractionation of the stem bark extract of Erythrina variegata L. has resulted in the isolation of three new isoflavones: 5,4'-dihydroxy-8-(3,3-dimethylallyl)-2''-methoxyisopropylfurano[4,5:6,7]isoflavone (1), 5,7,4'-trihydroxy-6-(3,3-dimethylallyloxiranylmethyl)isoflavone (2), 5,4'-dihydroxy-8-(3,3-dimethylallyl)-2''-hydroxymethyl-2''-methylpyrano[5,6:6,7]isoflavone (3) and a new isoflavanone, 5,4'-dihydroxy-2'-methoxy-8-(3,3-dimethylallyl)-2'',2''-dimethylpyrano[5,6:6,7]isoflavanone (4) together with seven known compounds, euchrenone b10 (5), isoerysenegalensein E (6), wighteone (7), laburnetin (8), lupiwighteone (9), erythrodiol (10), and oleanolic acid (11). The structures were determined on the basis of spectroscopic analyses and chemical evidence. The effect of these compounds on the proliferation of rat osteogenic sarcoma (UMR106) is also reported. Four new isoflavonoids from the stem bark of Erythrina variegata. Xiaoli L1, Naili W, Sau WM, Chen AS, Xinsheng Y. No
16598148
Cinnamolid-3beta-ol hemihydrate and 3beta-hydroxycinnamolide acetate, two drimanolide-class sesquiterpene lactones from Warburgia ugandensis. Zhang YX1, Jackson SH, Rajab MS, Fronczek FR, Watkins SF. No
16600543 Plagiochasma appendiculatum (Aytoniaceae) of the order Marchantiales is widely used in the form of paste ethnomedicinally by Gaddi tribe in Kangra valley for treating skin diseases. In this context, antimicrobical potential of Plagiochasma appendiculatum against a wide range of microorganisms was studied. To validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing activity. The plant (alchoholic and aqueous extract) showed significant antibacterial and antifungal activity against almost all the organisms: Micrococcus luteus, Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Streptococcus pneumoniae, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhimurium, and eight fungi Candida albicans and Cryptococcus albidus-dimorphic fungi, Trichophyton rubrum-dermatophyte fungi, Aspergillus niger, Aspergillus flavus, Aspergillus spinulosus, Aspergillus terreus and Aspergillus nidulans-systemic fungi, with especially good activity against the dermatophyte (Trichophyton rubrum) and some infectious bacteria (Escherichia coli, Proteus mirabilis and Salmonella typhimurium) with an MIC of 2.5 microg/disc. The results show that Plagiochasma appendiculatum extract has potent wound healing capacity as evident from the wound contraction and increased tensile strength. The results also indicated that Plagiochasma appendiculatum extract possesses potent antioxidant activity by inhibiting lipid peroxidation and increase in the superoxide dismutase (SOD) and Catalase activity. Antimicrobial, wound healing and antioxidant activity of Plagiochasma appendiculatum Lehm. et Lind. Singh M, Govindarajan R, Nath V, Rawat AK, Mehrotra S. No
16643024 The first chemical study on the stems and leaves of Daphniphyllum yunnanense led to the isolation of five new alkaloids, daphniyunnines A-E (1-5). Daphniyunnines B-E (2-5) are four unusual C-22 nor-Daphniphyllum alkaloids. The structures of these alkaloids were characterized by spectroscopic methods, especially 2D NMR techniques. A single-crystal X-ray diffraction analysis was used to confirm the structure of 1. Daphniyunnine D (4) showed cytotoxicity against two tumor cell lines, P-388 and A-549, with IC(50) values of 3.0 and 0.6 microM, respectively. Daphniyunnines A-E, alkaloids from Daphniphyllum yunnanense. Zhang H1, Yang SP, Fan CQ, Ding J, Yue JM. No
16643044 Six new nortriterpenoids, lancifodilactones I-N (1-6), as well as nine known ones, were isolated from the leaves and stems of Schisandra lancifolia. Their structures were elucidated on the basis of spectroscopic methods including 2D NMR analysis, and the structures of compounds 1 and 4 were further confirmed by single-crystal X-ray crystallography. In addition, all new compounds were tested for anti-HIV-1 activity. Nortriterpenoids from Schisandra lancifolia. Xiao WL, Huang SX, Zhang L, Tian RR, Wu L, Li XL, Pu JX, Zheng YT, Lu Y, Li RT, Zheng QT, Sun HD. No
16651783 A new prenylated xanthone, 5-O-methylcelebixanthone (1), together with six known compounds; celebixanthone (2), 1,3,7-trihydroxy-2,4-di(3-methylbut-2-enyl)xanthone (3), cochinchinone A (4), alpha-mangostin (5), beta-mangostin (6) and cochinchinone C (7) were isolated from roots of Cratoxylum cochinchinense. Their structures were elucidated by spectroscopic methods. Compounds 2 and 4-7 showed cytotoxic activity against the human lung cancer cell line (NCI-H187) with IC(50) values ranging from 0.65 to 5.2 microg/ml. Compounds 1, 2, 6 and 7 also showed antimalarial activity against Plasmodium falciparum with IC(50) values of 3.2, 4.9, 7.2 and 2.6 microg/ml, respectively. Cytotoxic and antimalarial prenylated xanthones from Cratoxylum cochinchinense. Laphookhieo S, Syers JK, Kiattansakul R, Chantrapromma K. No
16678369 A series of 61 Indian medicinal plants belonging to 33 different families used in various infectious disorders, were screened for their antimicrobial properties. Screening was carried out at 1000 and 500 microg/ml concentrations by agar dilution method against Bacillus cereus var mycoides, Bacillus pumilus, Bacillus subtilis, Bordetella bronchiseptica, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus faecalis, Candida albicans, Aspergillus niger and Saccharomyces cerevisiae. Twenty-eight plant extracts showed activity against at least one of the test organisms used in the screening. On the basis of the results obtained, we conclude that the crude extracts of Dorema ammoniacum, Sphaeranthus indicus, Dracaena cinnabari, Mallotus philippinensis, Jatropha gossypifolia, Aristolochia indica, Lantana camara, Nardostachys jatamansi, Randia dumetorum and Cassia fistula exhibited significant antimicrobial activity and properties that support folkloric use in the treatment of some diseases as broad-spectrum antimicrobial agents. This probably explains the use of these plants by the indigenous people against a number of infections. Search for antibacterial and antifungal agents from selected Indian medicinal plants Kumar VP, Chauhan NS, Padh H, Rajani M. No
16678870 Tetraoxygenated xanthones, cowaxanthones A-E, together with 10 previously reported tetraoxygenated xanthones, were isolated from the crude hexane extract of the fruits of Garcinia cowa. Cowaxanthone B has previously been reported as a synthetic xanthone. Their structures were elucidated by analysis of spectroscopic data, especially by 1D and 2D NMR. The antibacterial activities of the isolated compounds were also evaluated. Tetraoxygenated xanthones from the fruits of Garcinia cowa. Panthong K, Pongcharoen W, Phongpaichit S, Taylor WC. No
16683532 AIM: To study the chemical constituents of the fruits of Psoralea corylifolia L.METHODS: The constituents were separated and purified by column chromatography with normal phase silica gel and polyamide. Their structures were identified on the basis of physical and spectral data (MS, NMR, HMBC and HMQC).RESULTS: Six compounds were isolated and identified as psoralen (1), isopsoralen (2), bavachalcone (3), psoralidin (4), daidzin (5) and bavadin (6).CONCLUSION: Compound 6 is a new compound. Compound 5 was isolated from this plant for the first time. [Isolation and structure identification of a new isoflavone from Psoralea corylifolias]. Yang TT, Qin MJ. No
16711423 OBJECTIVE: To study the flavonoids of the aerial parts of Andrographis paniculata.METHOD: Twelve flavonoids from the 85% EtOH extract were isolated by the silica gel column, Sephadex LH-20 column, ODS column chromatography and HPLC, and their structures were identified by the spectal analyses and chemical evidences.RESULT: The 12 compounds were obtained and identified as 7-O-methylwogonin (1), 7-O-methyldihydrowogonin (2), 5-hydroxy-7, 8, 2', 5'-tetramethoxy-flavone (3), skullcapflavone-2'-methoxylether (4), 5-hydroxy-7, 8, 2', 3'-tetramethoxyflavone (5), 5, 4'-dihydroxy-7, 8, 2', 3'-tetramethoxyflavone (6), dihydroskullcapflavone (7), 5, 7, 8-trimethoxydihydroflavone (8), 5, 2'-dihydroxy-7, 8-dimethoxyflvone (9), andrographidine C (10), 5, 7, 4'-trihydroxyflavone (11), 5, 7, 3', 4'-tetrahydroxyflavone (12).CONCLUSION: Compound 6 is a new naturally occurring product isolated from A. paniculata, and compound 8, 11 and 12 are obtained for the first time from the Andrographis species. [Studies on flavonoids of Andrographis paniculata]. Chen LX, Qu GX, Qiu F. No
16711424 OBJECTIVE: To study the flavone constituents in Chrozophora sabulosa (Xinjiang origin).METHOD: The compounds were extracted with 95% ethyl alcohol, isolated by various column chromatography and identified by spectroscopic methods.RESULT: Seven flavanoids were isolated and identified as quercetin (I), kaempferol (II), apigenin (III), chrysoerid (IV), isoquercitrin (V), chrysoerin-7-O-beta-D-glucoside (VI) and quercetin-3-O-alpha-D-arabinfuranoside (VII).CONCLUSION: All of these seven compounds were obtained from this genus for the first time. [Studies on the flavones in of Chrozophora sabulosa]. Shi XH, Liu YQ, Kong LY. No
16711425 OBJECTIVE: To study the antiviral constituents in the stems and leaves of Pithecellibium clypearia.METHOD: The constituents of P. clypearia were systematically separated with various chromatographic techniques in combination with antiviral activity monitoring. Their structures were elucidated by physical and chemical properties and spectral data.RESULT: Six compounds were isolated from P. clypearia and were identified as: tricetiflavan (5, 7, 3', 4', 5'-pentahydroxylflavan) (1), myricitrin (myricetin-3-O-alpha-L-rhamnopyranoside) (2), quercitrin (quercetin-3-O-alpha-L-rhamnopyranoside) (3), quereetin (4), methyl gallate (5) and gallic acid (6).CONCLUSION: Compound 1 approximately 5 were obtained from this plant for the first time. Compound 4 was found to show an obvious anti-respiratory syncytial virus (RSV) activity. [Studies on antiviral constituents in stems and leaves of Pithecellibium clypearia]. Li YL, Li KM, Su MX, Leung KT, Chen YW, Zhang YW. No
16713157 Antimalarial activity of 10 vegetal extracts (9 ethanolic extracts and 1 crude alkaloid extract), obtained from eight species traditionally used in Colombia to treat malaria symptoms, was evaluated in culture using Plasmodium falciparum chloroquine resistant (FcB2) strain and in vivo on rodent malaria Plasmodium berghei. The activity on ferriprotoporphyrin biomineralization inhibition test (FBIT) was also assessed. Against Plasmodium falciparum, eight extracts displayed good activity Abuta grandifolia (Mart.) Sandwith (Menispermaceae) leaves, Acacia farnesiana (L.) Willd. (Mimosaceae) leaves, Acnistus arborescens (L.) Schltdl. (Solanaceae) aerial part, Croton leptostachyus Kunth (Euphorbiaceae) aerial part, Piper cumanense Kunth (Piperaceae) fruits and leaves, Piper holtonii C. DC. (Piperaceae) aerial part and Xylopia aromatica (Lam.) Mart. (Annonaceae) bark with IC(50) values ranging from <1 to 2.1 microg/ml, while in the in vivo model only Abuta grandifolia alkaloid crude extract exhibits activity, inhibiting 66% of the parasite growth at 250 mg/kg/day. In the FBIT model, five extracts were active (Abuta grandifolia, Croton leptostachyus, Piper cumanense fruit and leaves and Xylopia aromatica). Antimalarial activity of some Colombian medicinal plants. Garavito G, Rincón J, Arteaga L, Hata Y, Bourdy G, Gimenez A, Pinzón R, Deharo E. No
16722376 OBJECTIVE: To study the chemical constituents of Myricaria bracteata.METHOD: The chemical constituents were isolated by silica gel column chromatography and the structures were elucidated by spectroscopic methods.RESULT: Eleven compounds were obtained and identified as rhamnetin, 3,5,4'-trihydroxy-7,3'-dimethoxyflavone, 3,5,4'-trihydroxy-7-methoxyflavone, quercetin-3-O-alpha-L-rhamnopyranoside, kaempferol, quercetin, chrysoerio, gallic acid, gallic acid ethylester, beta-sitosterol, daucosterol.CONCLUSION: All compounds were obtained from M. bracteata for the first time. [Studies on chemical constituents in herb of Myricaria bracteata]. Zhou R, Wang T, Du XZ. No
16724853 Two new antiviral flavan derivatives were isolated from a methanol extract of leaves of Pithecellobium clypearia as guided by antiviral assays. The structures were characterized, by spectroscopic analyses, as 7-O-galloyltricetifavan (1) and 7,4'-di-O-galloyltricetifavan (2). Cytopathic effect (CPE) reduction assay showed that both compounds 1 and 2 possess antiviral activity against respiratory syncytial virus (RSV), with 50% inhibition concentration (IC(50)) values of 5 and 10 microg/mL, respectively; influenza A (H1N1) virus, with IC(50) values of 15.7 and 30 microg/mL; Coxsackie B3 (Cox B3) virus, with IC(50) values of 12.5 and 25 microg/mL, respectively; and Herpes simplex virus type 1 (HSV-1) with IC(50) values of 30 and 20 microg/mL, respectively. Cytotoxicity evaluation using the MTT assay showed that both compounds 1 and 2 were also moderately toxic to several cultured cell lines. Antiviral flavans from the leaves of Pithecellobium clypearia. Li Y1, Leung KT, Yao F, Ooi LS, Ooi VE.
16740282 A phytochemical investigation of the constituents of the roots of Vismia laurentii has resulted in the isolation of two xanthone derivatives named laurentixanthone A (1) (6-hydroxy-3,3-dimethyl-11-(3-methylbut-2-enyl)pyrano[2,3-c]xanthen-7(3H)-one) and laurentixanthone B (2) (1-hydroxy-5,6,7,8-tetramethoxyxanthone), along with 11 known compounds: 1,7-dihydroxyxanthone, vismiaquinone, vismiaquinone B, bivismiaquinone, 3-geranyloxy-6-methyl-1,8-dihydroxyanthraquinone, O(1)-demethyl-3',4'-deoxypsorospermin-3',4'-diol, 6-deoxyisojacareubin, 1,8-dihydroxy-6-methoxy-3-methylanthraquinone, kaempferol, friedelin and stigmasterol. The structures of compounds were established by means of spectroscopic methods. Furthermore, the compounds were screened for antimicrobial activities in vitro. Laurentixanthones A and B, antimicrobial xanthones from Vismia laurentii. Nguemeving JR, Azebaze AG, Kuete V, Eric Carly NN, Beng VP, Meyer M, Blond A, Bodo B, Nkengfack AE. No
16753781 Three new xanthones, 3, 6-dihydroxy-1, 2, 7-trimethoxyxanthone (2), 3, 7-dihydroxy- 1, 2-dimethoxyxanthone (4) and 1, 2, 7-trihydroxy-3-methoxyxanthone (9), together with seven known compounds were isolated from the roots of Polygala japonica Houtt. Their structures were determined on the basis of spectroscopic data. Three new xanthones from the roots of Polygala japonica Houtt. Fu J, Zhang DM, Chen RY. No
16755064 Further investigation of the dichloromethane extract of the twigs of Mammea siamensis led to the isolation of four novel coumarins, named siamenols A-D (1-4) together with three known xanthones. The structures of the new coumarins were elucidated by spectroscopic analysis, including 2D NMR spectroscopic data. In addition, the absolute stereochemistry of hydroxyl group of siamenol C (3) was determined to be S configuration by using modified Mosher's method. Siamenols A-D, four new coumarins from Mammea siamensis. Prachyawarakorn V, Mahidol C, Ruchirawat S. No
16758994 AIM: To investigate the glycosidic constituents in the rhizomes of Alpinia officinarum Hance.METHODS: The isolation and purification of glycosides were done with column chromatography on macro porous resin, polyamides and Sephadex LH-20, whilst the structure elucidation was done by HRCI-MS and NMR (1D and 2D) methods.RESULTS: A glycosidic ester identified as 4'-hydroxy-2'-methoxyphenol-beta-D-{6-0-[4"-hydroxy-3", 5"-dimethoxy (benzoate)]}-glucopyranoside (I), along with a known compound n-butyl-beta-D-fructopyranoside (II), were isolated and characterized.CONCLUSION: I was found to be a new compound, named as alpinoside A, whilst II was isolated from the genus Alpinia for the first time A new glycoside from Alpinia officinarum. An N, Lin J, Yang SL, Zou ZM, Xu LZ. No
16869487 The fresh leaves and brine of leaves of Thymbra spicata var. spicata (KARAKIZ) were analyzed by hydrodistillation, headspace and GC/MS techniques. The main components were determined as carvacrol, p-cymene, beta-myrcene, gamma-terpinene, a-terpinene and trans-caryophyllene. The essential oil and the main compounds, carvacrol and trans-caryophyllene, have been tested against E. coli, S. epidermidis, B. subtilis, S. aureus, S. typhimurium, K. pneumoniae, P aeruginosa, E. faecalis and C. albicans. While the essential oil and carvacrol showed strong activity against all microorganisms, except P. aeruginosa, trans-caryophyllene showed activity only against C. albicans. The essential oil and carvacrol also showed strong antimycobacterial activity. Analysis of essential oil composition of Thymbra spicata var. spicata: antifungal, antibacterial and antimycobacterial activities. Kiliç T. No
16882158 AIMS: The aim of the present study was to investigate the anti-Streptococcus mutans activity and the in vitro effects of subminimal inhibitory concentrations of guaijaverin isolated from Psidium guajava Linn. on cariogenic properties of Strep. mutans.METHODS AND RESULTS: Bioautography-directed chromatographic fractionation, yield biologically active compound, quercetin-3-O-alpha-l-arabinopyranoside (guaijaverin), from crude methanol extract of P.guajava. Growth-inhibitory activity of the compound against Strep. mutans of both clinical and type strain cultures was evaluated. The anti-Strep. mutans activity of the guaijaverin was found to be bacteriostatic, both heat and acid stable and alkali labile with the minimum inhibitory concentration (MIC) of 4 mg ml(-1) for MTCC 1943 and 2 mg ml(-1) for CLSM 001. The sub-MIC concentrations (0.0078-2 mg ml(-1)) of the guaijaverin were evaluated for its cariogenic properties such as acid production, cell-surface hydrophobicity, sucrose-dependent adherence to glass surface and sucrose-induced aggregation of Strep. mutans.CONCLUSIONS: The active flavonoid compound, quercetin-3-O-alpha-l-arabinopyranoside (guaijaverin) demonstrated high potential antiplaque agent by inhibiting the growth of the Strep. mutans.SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrated the new growth-inhibitory compound guaijaverin against Strep. mutans and led to the acceptance of traditional medicine and natural products as an alternative form of health care. Guaijaverin -- a plant flavonoid as potential antiplaque agent against Streptococcus mutans. Prabu GR, Gnanamani A, Sadulla S. No
16884745 Six xanthones, 1,3,7-trihydroxy-2-(2-hydroxy-3-methyl-3-butenyl)-xanthone (1), 1,7-dihydroxy-2,3-[2''-(1-hydroxy-1-methylethyl)-dihydrofurano]-xanthone (2), 1,3,7-trihydroxy-5-methoxyxanthone (3), 1,7-dihydroxy-5,6-dimethoxyxanthone (4), 4,5-dihydroxy-2,3-dimethoxyxanthone (5), 1,3-dihydroxy-2,4-dimethoxyxanthone (6) and 21 known xanthones were isolated from the leaves and stems of Hypericum chinense. Their structures were established based on spectroscopic studies. Xanthones from Hypericum chinense. Tanaka N, Takaishi Y. No
16973190 An investigation of an ethyl acetate extract of Embelia schimperi leaves has led to the isolation of 10 oleanane-type triterpenes characterized as 3beta,16alpha-di-O-acetyl-13beta, 28-epoxyoleanane (1), 3beta-acetyl-16-oxo-13beta, 28-epoxyoleanane (2), 3beta-acetyl-16alpha-hydroxy-13beta, 28-epoxyoleanane (3), 3beta-acetyl-16alpha-hydroxyoleanane-13beta, 28-olide (4), 3beta-acetyl-28-hydroxy-16-oxo-12-oleanene (5), 3beta, 28-di-O-acetyl-16alpha-hydroxy-12-oleanene (6), 3beta-acetyl-11alpha, 28-dihydroxy-16-oxo-12-oleanene (7), 3beta, 11alpha, 16alpha, 28-tetrahydroxy-12-oleanene (8), 3beta-acetyl-16alpha, 28alpha-dihydroxy-13beta, 28-oxydooleanane (9) and 3beta, 28alpha-dihydroxy-16-oxo-13beta, 28-oxydooleanane (10). The known compounds isolated from the same extract included 3beta, 16alpha-dihydroxy-13beta, 28-epoxyoleanane (protoprimulagenin A) (11), 3beta-hydroxy-16-oxo-13beta, 28-epoxyoxyoleanane (aegicerin) (12), 3, 16-dioxo-13beta, 28-epoxyoleanane (embilionone) (13), 3beta, 28-dihydroxy-16-oxo-12-oleanene (schimperinone) (14), taraxerone (15), taraxerol (16) and stigmasterol (17). Structure elucidations were carried out spectroscopically. Oleanane-type triterpenes of Embelia schimperi leaves. Manguro LO, Okwiri SO, Lemmen P. No
16989532 Three new alkaloids, gelsebanine (1), 14alpha-hydroxyelegansamine (2), and 14alpha-hydroxygelsamydine (3), and a new extraction artifact , gelsebamine (4), together with 12 known alkaloids, were isolated from the stems and leaves of Gelsemium elegans. The structures of 1-4 were determined by spectroscopic methods, especially 2D NMR techniques. Compounds 1-4 were evaluated for cytotoxic activity against four tumor cell lines, and gelsebamine (4) selectively inhibited the A-549 human lung adenocarcinoma cell line. Alkaloids from Gelsemium elegans. Xu YK1, Yang SP, Liao SG, Zhang H, Lin LP, Ding J, Yue JM. No
17019779 One hundred ninety hexanic and ethanolic extract from 27 plant species from the Cerrado biome of Brazil were tested for larvicidal activity against 3rd-stage Aedes aegypti larvae at 500 microg/ml. Fourteen extracts from 7 species showed activity (>65% mortality) against the larvae. Of these Dugeutia furfuracea, Piptocarpha rotundifolia, Casearia sylvestris var. lingua, Serjania lethalis, and Xylopia aromatica were active at 56.6, 162.31, 232.4, 285.76, and 384.37 microg/ml, respectively. Annona crassiflora and Cybistax antisyphilitica showed activity at 23.06 and 27.61 microg/ml. The larvicidal properties of these species are described for the first time, and may prove to be promising in active chemical compound isolation. Larvicidal activity of some Cerrado plant extracts against Aedes aegypti. Rodrigues AM, De Paula JE, Degallier N, Molez JE, Espindola LS. No
17034775 The antiangiogenic effect of Lygodium flexuosum extract was evaluated in Wistar rats intoxicated with N-nitrosodiethylamine (NDEA) in preventive and curative models. In preventive groups, NDEA was administered for 20 weeks. Daily doses of L. flexuosumn-hexane extract (200mg/kg) started 1 week before the onset of NDEA intoxication and continued for 20 weeks. In curative animals, NDEA was administered for 20 weeks followed by treatment with the n-hexane extract of L. flexuosum for 28 days. Rats intoxicated with NDEA had elevated levels of serum gamma-GT, AST, ALT, LDH levels and hepatic MDA and decreased levels of hepatic GSH. When treated with L. flexuosum extract had normal levels of gamma-GT, AST, ALT, LDH levels, hepatic MDA and GSH. NDEA administered rat liver showed an overexpressed levels of angiopoietins 1 (Ang-1) and 2 (Ang-2) and its receptor Tie-2 mRNA. L. flexuosum extract treatment significantly (p<or=0.05) reduced the levels of Ang-1 and Ang-2 and Tie-2 in rat livers evidenced by RT-PCR. Immunohistochemical analysis showed that vascular endothelial growth factor (VEGF) was overexpressed and localized around periportal area of liver sections intoxicated with NDEA and its overexpression was effectively reduced by the treatment with L. flexuosum extract. Histopathological observations also substantiated NDEA-induced hepatotoxicity and the effect was significantly (p<or=0.05) reduced by L. flexuosum extract treatment. Thus, L. flexuosum extract at a dose of 200mg/kg effectively reversed the hepatotoxicity induced by N-nitrosodiethylamine in both experimental models. Antiangiogenic effect of Lygodium flexuosum against N-nitrosodiethylamine-induced hepatotoxicity in rats Wills PJ, Suresh V, Arun M, Asha VV No
17048566 OBJECTIVE: To study the phenolic constituents of the leaves of pineapple.METHOD: Chromatographic methods were used to isolate compounds from the leaves of pineapple and spectroscopic methods were used to identify the structures of the isolated compounds.RESULT: 7 compounds, ananasate (1), 1-O-caffeoylglycerol (2), 1-O-p-coumaroylglycerol (3), caffeic acid (4), p-coumaric acid (5), beta-sitosterol (6) and daucosterol (7), were isolated from the leaves of pineapple.CONCLUSION: 1 was a new compound, and others were obtained from this plant for the first time. [Studies on phenolic constituents from leaves of pineapple (Ananas comosus)]. Wang W, Ding Y, Xing DM, Wang JP, Du LJ. No
17048586 OBJECTIVE: To investigate the constituents of Ervatamia hainanensis systematically.METHOD: Various chromatographic techniques were applied to isolate and purify the constituents of this plant. The structures were elucidated by spectroscopic analysis.RESULT: Eight compounds were obtained, which were identified as alpha-amyrin acetate (1), 11-oxo-alpha-amyrin acetate (2), beta-sitosterol (3), cycloart-23-ene-3beta, 25-diol(4), cycloart-25-ene-3beta, 24-diol (5), 5alpha, 8alpha-epidioxyergosta-6, 22-dien-3beta-ol (6), ibogamin-3-one (7), beta-daucosterol (8).CONCLUSION: Compounds 1, 2, 4- 7 were isolated from this plant for the first time. [Studies on constituents from root and stem of Ervatamia hainanensis]. Huang JP, Ma YM, Jiang JS, Zhang PC. No
17048605 OBJECTIVE: To study the active constituents from Alternanthera philoxeroides.METHOD: The constituents were isolated with silica gel and Toyopearl HW-40C gel column chromatography and purified by HPLC. Their structures were elucidated by spectroscopy.RESULT: Nine compounds were isolated and identified as phaeophytin a (1), pheophytin a' (2), oleanoic acid (3), beta-sitosterol (4), 3beta-hydroxystigmast-5-en-7-one (5), alpha-spinasterol (6), 24-methylenecycloartanol (7), cycloeucalenol (8), phytol (9).CONCLUSION: Compounds 1,2,5,7-9 were isolated from this plant for the first time. [Chemical constituents from herb of Alternanthera philoxeroides]. Fang JB, Duan HQ, Zhang YW, Yoshihisa T. No
17049570 The chemical investigation of leaves of Bridelia glauca f. balansae afforded six megastigmane glucosides, named bridelionosides A-F, along with seven known megastigmane glucosides. Their structures were determined by a combination of spectroscopic analyses and by application of the modified Mosher's method. Bridelionosides A-F: Megastigmane glucosides from Bridelia glauca f. balansae. Sueyoshi E1, Liu H, Matsunami K, Otsuka H, Shinzato T, Aramoto M, Takeda Y. No
17061555 OBJECTIVE: To study the flavonoids in the leaves of Apocynum ventum.METHOD: Compounds were separated by repeated polyacrylamide column chromatgraphy combined with Sephadex LH-20 column chromatgraphy, their structures were confirmed by the analysis of spectral data and physicochemical properties.RESULT: Six compounds were isolated from A. ventum. They were elucidated as quercetin (1), kaempferol (2), kaempferol-3-O-(6"-O-acetyl)-beta-D-glucopyranoside (3), quercetin-3-O-(6"-O-acetyl )-beta-D-glucopyranoside (4), quercetin-3-O-(6"-O-acetyl)-beta-D-galactopyranoside (5), kaempferol-7-O-alpha-L-rhamnopyranoside (6).CONCLUSION: Compound 5 and 6 were isolated from the genus Apocynum for the first time. [Study on flavonoids in leaves of Apocynum ventum]. Li LH, Yuan Z. No
17067171 Three new 9,19-cycloartane triterpene glycosides, asiaticoside A (1), asiaticoside B (2), and 25-O-ethylcimigenol-3-O-beta-D-xylopyranoside (3), together with cimiacemoside I (4), 25-O-acetylcimigenol-3-beta-O-D-xyloside (5), and 25-anhydrocimigenol-beta-O-D-xyloside (6) were isolated from the roots/rhizomes extract of Actaea asiatica, and their structures were established by spectroscopic methods (IR, HRESIMS, and NMR). Compounds 1-3, 5, and 6 had notable cytotoxicity against HepG2 and MCF-7 cancer cell lines. Cytotoxic cycloartane triterpene saponins from Actaea asiatica. Gao J, Huang F, Zhang J, Zhu G, Yang M, Xiao P. No
17076236 OBJECTIVE: To study the chemical constituents from Serissa serissoides DC. roots.METHODS: Seven compounds were obtained from the 80% ethanol extract of Serissa serissoides roots by repeated column chromatography over silica gel, Sephadex LH-20 and Polyamide. Their structures were identified by analysis of their spectral data and physics character.RESULTS: Seven compounds obtained from the plant leaves were identified as: (+)-pinoresinol(1), (-)-syringaresinol( 2), (+)-meduiresinol(3), (-)-olivil(4), beta-sitosterol(5), oleanolic acid(6) and daucosterol(7).CONCLUSION: They are all known compounds. Compound 2 and 4 was obtained from this genus plants for the first time, and compounds 1,3 and 7 as known compounds were obtained from the root of the plant firstly. [Studies on chemical constituents of Serissa serissoides roots]. Zhang Q, Sun LR. No
17113739 Phyllanthus urinaria Linnea (Euphorbiaceae) is a widely used traditional medicinal plant by oriental countries and has been reported to possess various biological activities. Previously, the acetone extract from Phyllanthus urinaria was found to inhibit herpes simplex virus (HSV) infection. In this study, geraniin and 1,3,4,6-tetra-O-galloyl-beta-D-glucose (1346TOGDG), both of which were isolated from the acetone extract of Phyllanthus urinaria, were examined for their activity against HSV-1 and HSV-2 in vitro. Results showed that geraniin actively suppressed HSV-2 infection, whereas 1346TOGDG effectively inhibited HSV-1 infection. The 50% inhibitory concentration (IC(50)) was 18.4+/-2.0 microM for geraniin against HSV-2 infection, and 19.2+/-4.0 microM for 1346TOGDG against HSV-1. No toxic effect towards the host cell was observed at the antiviral concentrations. In conclusion, geraniin and 1346TOGDG were found to inhibit HSV-1 and HSV-2 multiplication at different magnitudes of potency. The in vitro activity of geraniin and 1,3,4,6-tetra-O-galloyl-beta-D-glucose isolated from Phyllanthus urinaria against herpes simplex virus type 1 and type 2 infection. Yang CM, Cheng HY, Lin TC, Chiang LC, Lin CC. No
17125227 Four new triterpene saponins, clematomandshurica saponins A-D (1-4), together with three known saponins (5-7) have been isolated from the roots and rhizomes of Clematis mandshurica. Their structures were elucidated on the basis of their spectroscopic evidence and hydrolysis. Clematomandshurica saponins A and B showed significant inhibitory activity on cyclooxygenase-2 (IC50 = 2.66 and 2.58 microM, respectively). Triterpene saponins from clematis mandshurica. Shi S, Jiang D, Dong C, Tu P. No
17125240 Three new acylphloroglucinol derivatives have been isolated from the hexane extract of the aerial parts of Hypericum prolificum L.: prolificin A (1), prolifenone A (2), and prolifenone B (3). The structures were elucidated on the basis of extensive 2D NMR and MS data. All three compounds were evaluated for in vitro cell proliferation inhibitory activity against human breast (MCF-7), lung (NCI-H460), CNS (SF-268), stomach (AGS), and colon (HCT-116) tumor cell lines. Prolificin A showed growth inhibition of all cell lines with IC50 values ranging from 23 to 36 microM. Prolifenones A and B were inactive at the concentrations tested. Acylphloroglucinol derivatives from Hypericum prolificum. Henry GE, Raithore S, Zhang Y, Jayaprakasam B, Nair MG, Heber D, Seeram NP. No
17127518 From aerial parts of Swertia longifolia Boiss., which grows in the north of Iran, five xanthones, two of which in diglycosidic form, were isolated. The structures were confirmed by means of their spectral data as isobellidifolin, bellidin, gentisein, 1,5-dihydroxy-3-methoxy-6-O-primeverosyl xanthone, and 8-hydroxy-3,5-dimethoxy-1-O-primeverosyl xanthone, the latter two of which were new derivatives in the plant kingdom. Two new xanthone diglycosides from Swertia longifolia Boiss. Hajimehdipoor H, Dijoux-Franca MG, Mariotte AM, Amanzadeh Y, Sadat-Ebrahimi SE, Ghazi-Khansari M. No
17161920 The antifungal activity of methanolic extract and alkaloidal fraction of Berberis aetnensis against Candida species was investigated. The crude extract was active against Candida species, this activity being higher than that of the alkaloidal fraction and berberine. Activity of Berberis aetnensis root extracts on Candida strains. Iauk L, Costanzo R, Caccamo F, Rapisarda A, Musumeci R, Milazzo I, Blandino G. No
17165582 OBJECTIVE: To study the diterpenoids in the 85% ethanol extract of the aerial parts of Andrographis paniculata.METHOD: Fourteen diterpenoids from the 85% EtOH extract were isolated by the silica gel, Sephadex LH-20, ODS column chromatography and HPLC, and their structures were identified by the spectal analyses and chemical evidences.RESULT: The 14 compounds were obtained and identified as neoandrographolide (1), 3, 14-di-deoxyandrographolide (2), andrographolide (3), 14-deoxy-11, 12-didehydroandrographolide (4), 19-hydroxy-8 (17), 13-labdadien-15, 16-olide (5), 14-deoxy-andrographolide (6), 3-oxo-14-deoxy-andrographolide (7), isoandrographolide, (8), bisandrographolide (9, 10, 11), deoxyandrographiside (12), 14-deoxy-11, 12-didehydroandrographiside (13), andrographiside (14), respectively.CONCLUSION: Compound 7 was a new natural product, and compound 5 was obtained for the first time from the Andrographis species. [Studies on diterpenoids from Andrographis paniculata]. Chen LX, Qu GX, Qiu F. No
17174000 Essential oil extracted from the leaves of Chenopodium ambrosioides Linn. (Chenopodiaceae) was tested against the aflatoxigenic strain of test fungus Aspergillus flavus Link. The oil completely inhibited the mycelial growth at 100 microg/ml. The oil exhibited broad fungitoxic spectrum against Aspergillus niger, Aspergillus fumigatus, Botryodiplodia theobromae, Fusarium oxysporum, Sclerotium rolfsii, Macrophomina phaseolina, Cladosporium cladosporioides, Helminthosporium oryzae and Pythium debaryanum at 100 microg/ml. The oil showed significant efficacy in inhibiting the aflatoxin B1 production by the aflatoxigenic strain of A. flavus. During in vivo investigation it protected stored wheat from different storage fungi for one year. Chenopodium oil also exhibited potent antioxidant activity when tested by ABTS method. All these observations suggest the possible exploitation of the Chenopodium oil as potential botanical fungitoxicant in ecofriendly control of post harvest biodeterioration of food commodities from storage fungi. Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity. Kumar R, Mishra AK, Dubey NK, Tripathi YB. No
17188496 Three new Lycopodium alkaloids, carinatumins A-C (1-3), have been isolated from the club moss Lycopodium carinatum. Structures and stereochemistry of 1-3 were elucidated on the basis of 2D NMR correlations. Carinatumins A (1) and B (2) exhibited a potent inhibitory activity against acetylcholinesterase. Carinatumins A-C, new alkaloids from Lycopodium carinatum inhibiting acetylcholinesterase. Choo CY, Hirasawa Y, Karimata C, Koyama K, Sekiguchi M, Kobayashi J, Morita H. No
17190453 Five new yuzurimine-type alkaloids, yunnandaphnines A-E (2-6), together with two known analogues, macrodaphniphyllamine (1) and calycinine A (7), have been isolated from the leaves and twigs of Daphniphyllum yunnanense. The structures of the new alkaloids were elucidated by spectroscopic methods. Yunnandaphnine E (6) is a novel heptacyclic yuzurimine-type alkaloid with an oxazine ring. Yuzurimine-type alkaloids from Daphniphyllum yunnanense. Di YT1, He HP, Li CS, Tian JM, Mu SZ, Li SL, Gao S, Hao XJ. No
17191901 Three new isoflavonoids, eryvarins M-O (1-3), two new 2-arylbenzofurans, eryvarins P and Q (4 and 5), and a new 3-aryl-2,3-dihydrobenzofuran, eryvarin R (6), together with three known compounds, were isolated from the roots of Erythrina variegata. The structures of these compounds were established by spectroscopic analysis. Eryvarin R (6) is an unusual 3-aryl-2,3-dihydrobenzofuran derivative with a formyl (CHO) group. Eryvarin Q (5) showed potent antibacterial activity against methicillin-resistant Staphylococcus aureus. Six new constituents from the roots of Erythrina variegata. Tanaka H1, Hirata M, Etoh H, Sako M, Sato M, Murata J, Murata H, Darnaedi D, Fukai T. No
17202693 In our investigation on the chemical constituents of Hippophae rhamnoides L., the chloroform-soluble fraction of the 80% acetone extract of branch bark was observed to inhibit nitric oxide (NO) production in a lipopolysaccharide and recombinant mouse interferon-gamma-activated murine macrophage-like cell line, RAW 264.7 cells. Two new triterpenoids, 2-O-trans-p-coumaroyl maslinic acid (1) and 2-O-caffeoyl maslinic acid (2), and three known triterpenoids, oleanolic acid (3), 3-O-trans-p-coumaroyl oleanolic acid (4), and 3-O-caffeoyl oleanolic acid (5), and 6-methoxy-2H-1-benzopyran (6) and beta-sitosterol (7) were isolated from the branch bark extract. Their inhibitory activities on the production of NO in RAW 264.7 cells and radical-scavenging activities were examined. Triterpenoids from Hippophae rhamnoides L. and their nitric oxide production-inhibitory and DPPH radical-scavenging activities. Triterpenoids from Hippophae rhamnoides L. and their nitric oxide production-inhibitory and DPPH radical-scavenging activities. No
17212047 OBJECTIVE: To study the chemical constituents from dry roots of Ligusticum glaucescens Franch.METHODS: Chromatography and spectrum analysis were employed to isolate and elucidate the chemical constituents in the plant.RESULTS: 4 compounds were isolated and identified as levistolide A (I), ligustilide (II), pleuchiol (III) and ferulic acid (IV).CONCLUSION: I-IV were isolated from this plant for the first time. The work provided evidence for the exploitation and utilization of this plant resource. [Studies on chemical constituents from Ligusticum glaucescens Franch]. Chen JT, Yang CR. No
17225539 OBJECTIVE: To study the chemical constituents from the roots and rhizomes of Clematis hexapetala.METHOD: The compounds were separated by means of solvent extraction, repeated chromatography with silica gel and HPLC. The structures were determined by spectral analysis.RESULT: Nine compounds were isolated as friedelin (1) , anemonin (2) , beta-sitosterol (3) , palmitic acid (4) , vanillic acid (5) , isolariciresinol (6) , 5-hydroxumethyl-5H-furan-2-one (7) , n-nonane (8) , daucosterol (9).CONCLUSION: All the compounds were isolated from the plant for the first time. [Studies on chemical constituents from root of Clematis hexapetala]. Dong CX, Shi SP, Wu KS, Tu PF. No
17228656 OBJECTIVE: to study the chemical components of the leaves from Crataegus pinnatifida Bge. var major N. E. Br.METHODS: Constituents were isolated by using silica gel, porous resin, Sephadex LH-20 chromatographic technique, etc. Their structures were elucidated by chemical and spectroscopic methods.RESULTS: Fourteen compounds were identified as quercetin (1), hyperoside (2), quercetin 3-O-beta-D-glucoside (3), rutin (4), quercetin 3-O-[alpha-L-rhamnopyranosyl(1-4)-alpha-L-rhamnopyranosyl-(1-6)-beta-glucopyranoside] (5), vitexin (6), 6"-O-acetyl-vitexin (7), vitexin 2"-O-rhamnoside (8), vitexin 4"-O-glucoside (9), chlorogenic acid (10), ursolic acid (11), beta-sitosterol (12), beta-daucosterol (13), n-triacontanol (14).CONCLUSION: Compound 5 and 14 were isolated from Crataegus L. for the first time. [Study on the chemical constituents of the leaves from Crataegus pinnatifida Bge. var. major N. E. Br]. Liu RH, Yu BY. No
17253308 The defatted ethanolic extract of the fresh flowers of Pancratium maritimum L. yielded the four known alkaloids lycorine, maritidine, lycoramine, and galanthamine. The structures of the isolated alkaloids were determined mainly through spectroscopic studies including one- and two-dimensional NMR (COSY, NOESY, DEPT, HETCOR, and HMBC) and CD techniques. Some spectral data are newly reported or revised. Alkaloids of the flowers of Pancratium maritimum. Youssef DT, Frahm AW. No
17262260 Seven flavonol 3- O-glycosides were isolated from the flowers of CALENDULA OFFICINALIS L. Their structures were elucidated as isorhamnetin 3- O-glucoside, rutinoside, neohesperidoside, 2 (G)-rhamnosylrutinoside, quercetin glucoside, neohesperidoside, and 2 (G)-rhamnosylrutinoside by paper and thin layer chromatography, UV, (13)C-NMR, and mass spectroscopy. The interglycosidic linkages of isorhamnetin 3- O-neohesperidoside, 2 (G)-rhamnosylrutinoside, quercetin 3- O-neohesperidoside and structural determination of quercetin 2 (G)-rhamnosylrutinoside are described for the first time in CALENDULA OFFICINALIS. Flavonol glycosides from Calendula officinalis flowers. Vidal-Ollivier E, Elias R, Faure F, Babadjamian A, Crespin F, Balansard G, Boudon G No
17329884 Six new triterpenoid saponins of the ursane types were isolated from the MeOH extract of the leaves of Ilex oblonga. They were oblonganoside A (1), oblonganoside B (2), oblonganoside C (6), oblonganoside D (8), oblonganoside E (9), and oblonganoside F (10), together with three known triterpenoid saponins. The structures of these compounds were elucidated on the basis of spectroscopic analysis, and compound 1 showed appreciable inhibitory activity against TMV replication with EC50 value 0.074 mM. Six new triterpenoid saponins from the leaves of Ilex oblonga and their inhibitory activities against TMV replication. Wu ZJ1, Ouyang MA, Wang CZ, Zhang ZK. No
17329890 Two new flavonoid glycosides, 5-hydroxy-7,8-dimethoxy (2R)-flavanone-5-O-beta-D-glucopyranoside (1) and 5-hydroxy-7,8,2',5'-tetramethoxy-flavone-5-O-beta-D-glucopyranoside (2), and a new diterpenoid, andrographic acid (3), along with andrographidine A (4) were isolated from Andrographis paniculata, and their structures were determined on the basis of physicochemical and spectroscopic analysis. Compound 3 was evaluated for cytotoxicity to KB cells along with andrographolide, isoandrographolide, neoandrographolide and 14-deoxy-11,12-didehydroandrographolide obtained from A. paniculata in the present study. Cytotoxicity was observed for andrographolide and isoandrographolide with ED50 values of 6.5 and 5.1 microg/ml, respectively. Secondary metabolites from Andrographis paniculata. Li W, Xu X, Zhang H, Ma C, Fong H, van Breemen R, Fitzloff J. No
17329900 Four new triterpene glycosides, named nigellosides A, B, C, and D, were from the air-dried aerial parts of Nigella damascena L. (Ranunculaceae), and the structures were elucidated on the basis of spectroscopic data including 2D NMR spectra and chemical evidence. Their chemical structures have been characterized as 3-O-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranosyl gypsogenin 28-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl ester, 3-O-beta-D-glucopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranosyl gypsogenin 28-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl ester, 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-xylopyranosyl hederagenin 28-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl ester, and 3-O-beta-D-glucopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-xylopyranosyl hederagenin 28-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl ester. Four new triterpene glycosides from Nigella damascena. Yoshimitsu H1, Nishida M, Okawa M, Nohara T. No
17340259 Extracts of the roots of TINOSPORA CAPILLIPES Gagnep. (Menispertnaceae) afforded the quaternary protoberberine alkaloids palmatine, jatrorrhizine, columbamine, stepharanine and dehydrodiscretamine and the quaternary aporphine alkaloids menisperine and magnoflorine. Quaternary Alkaloids of Tinospora capillipes. Chang HM1, El-Fishawy AM, Slatkin DJ, Schiff PL Jr. No
17340262 The essential oil of NEPETA NEPETELLA L. growing in the Aosta Valley (Valnontey), Italy, obtained by steam distillation of the leaves and the flowers, was investigated by capillary gas chromatography in combination with mass spectrometry. To facilitate identification, fractionation of the oil with straight phase HPLC was performed. Nepetalactone (76.5%) is the main component of the oil. The isomers epinepetalactone (0.6%) and neonepetalactone (0.4%) together with the dihydro (1.6%) and dehydro (traces) nepetalactone derivatives were also identified. These compounds are considered to be responsible for the feline attractant activity of NEPETA NEPETELLA L. Constituents of Essential Oil of Nepeta nepetella. Bicchi C, Mashaly M, Sandra P. No
17342600 From the roots of UNCARIA TOMENTOSA six oxindol alkaloids have been isolated and identified as isopteropodin, pteropodin, mitraphyllin, isomitraphyllin, rynchophyllin and isorynchophyllin. By ion pair chromatography on HPLC a fingerprint analysis has been worked out which allowed to differentiate between various UNICARIA-species and drug preparations. With the exception of mitraphyllin and rynchophyllin all alkaloids showed a pronounced enhancement effect on phagozytosis, determined in two IN VITRO tests and the IN VIVO-carbon clearance test. Die Alkaloide von Uncaria tomentosa und ihre Phagozytose-steigernde Wirkung. Wagner H, Kreutzkamp B, Jurcic K. No
17348190 OBJECTIVE: To study the chemical constituents of the leaves of Isatis indigotica.METHOD: The leaves of I. indigotica were extracted with 80% ethanol. The EtOH extract was dispersed in H20 and extracted with petroleum, EtOAc and BuOH successively. The EtOAc fraction was isolated and purified by column chromatography on silica gel, Sephadex LH -20 and Rp-8, Rp-18. All the compounds were identified on the basis of spectral analyses (including MS, 1H-NMR, 13 C-NMR) , RESULT: Eleven compounds were isolated from the leaves of I. indigotica, and structures were characterized as 10H-indolo [3, 2-b] quinoline (1), indirubin (2), 4 (3H)-quinazo-linone (3), (E)-3-(3', 5'-dimethoxy-4'-hydroxybenzylidene) -2-indolinone (4), 2, 3-dihydropyrrolo [2, 1-b] quinazolin-9(1H) -one (5) , benzoic acid (6) , o-droxy-benzoic acid (7), ( - ) -lariciresinol (8) , ( + ) -isolariciresinol (9), isovitexin (10), 6-f-D-glucopyranosyldiosmetin (11).CONCLUSION: 1, 4, 5, 8, 9, 11 were obtained from the leaves of I. indigotica for the first time. [Studies on chemical constituents from leaves of Isatis indigotica]. Liu JF, Zhang XM, Xue DQ, Jiang ZY, Gu Q, Chen JJ. No
17348191 OBJECTIVE: To study the chemical constituents of radix and rhizome of Ligularia sagitta.METHOD: Isolation and purification were carried out on the column chromatography of silica gel and sephadex LH -20. The structures were identified by spectral analysis.RESULT: Seven terpenoid compounds were isolated from the radix and rhizome of L. sagitta. Five of them are sesquiterpenoids, the structures were determined as 7alpha-hydroxy-9(10) -ene-1, 8-dioxo-6, 7-dihydrofuranoeremophilane (1), 1p, 10P3-epoxy-6beta, 8 beta-dihydroxy-eremophil7 (11) -en-12, 8alpha-olide (2) , 1-oxo-9-desoxycacalol (3), benzofuranoeremophil-l-ene (4) and bakkenolide (5); other two compounds belong to triterpenoid. 3p, 16P3-dihydroxy-12-oleanen-28-al (6) and lupeol (7).CONCLUSION: Compoundsl-3 and 6 were: isolated from L. sagitta for the first time, and compounds 3 and 6 were obtained from Ligularia for the first time. [Terpenoid compounds isolated from Ligularia sagitta]. Liu SJ, Zhang M, Wang ZT, Liu JQ, Zhang CF. No
17365188 A new isoflavone, corylinin (1), along with six known compounds, isopsoralen (2), psoralen (3), sophoracoumestan A (4), neobavaisoflavone (5), daidzin (6) and uracil (7), have been isolated from the dried fruits of Psoralea corylifolia L. The structure of 1 was established as 7,4'-dihydroxy-3'-[(E)-3,7-dimethyl-2,6-octadienyl]isoflavone on the basis of the spectroscopic methods. Structures of the known compounds were identified by comparison of the literature. Studies on the chemical constituents of Psoralea corylifolia L. Ruan B, Kong LY, Takaya Y, Niwa M. No
17393660 A new xanthone named cratoxylumxanthone A (1), together with five known compounds: dulcisxanthone B (2), alpha-mangostin (3), beta-mangostin (4), 2-geranyl-1,3,7-trihydroxy-4-(3-methylbut-2-enyl)xanthone (5) and tectochrystin (6), was isolated from Cratoxylum cochinchinense stems. The structure of new compound was characterized by 1D and 2D NMR techniques. The isolated compounds showed free radical scavenging against DPPH and lipid peroxidation inhibition. Antioxidant xanthones from Cratoxylum cochinchinense. Phuwapraisirisan P, Udomchotphruet S, Surapinit S, Tip-Pyang S. No
17404954 In addition to the alkaloids canthin-6-one; dictamnine, chelerythrine, gamma-fagarine, skimmianine, magnoflorine and 11-(2'-ketobutane)-dihydrochelerythrine, four further alkaloids were isolated from the bark of Fagara mayu (Bert. ex Hook et Arn.) Engler and edulinine, bocconoline, tembetarine were identified. A new base, N-methylpseudolaudanine, was also isolated. Further alkaloids from bark of Fagara mayu. Assem EM1, Benages IA, Albonico SM. No
17447394 Abstarct:"OBJECTIVE:In traditional medical practices of Ethiopia the aqueous extracts obtained from the stem bark of Combretum molle (R. Br. Ex. G. Don.) Engl & Diels (Combretaceae) have a longstanding reputation for the treatment of liver diseases malaria and tuberculosis. Owing to the widespread traditional uses of this plant, the studyinvestigated the antimicrobial activity the bark extract of this plant against Gram positive and and Gram negative bacteria.METHODS:Petroleum ether, dichloromethane and acetone fractions of the bark of the plant were prepared by soxhlet extraction and screened for their antimicrobial activity. The acetone fraction exhibited a powerful activity and was therefore further tested against twenty-one bacterial and six fungal strains. The minimum inhibitory concentration (MIC) of this extract was determined by checker board technique using nutrient agar medium. The zones of inhibition produced by the extract against bacteria and fungi were determined and compared by disc diffusion technique with those of pure ciprofloxacin and griseofulvin, respectively.RESULTS:The highest antibacterial action of the acetone extract was against the Gram negative organisms EscherIchia coli and Shigella spp with an MIC value of 50 mg/ml. The activity of the extract against these bacteria was comparable to that of ciprofloxacin when assessed by the disc diffusion technique. Among the fungal strains tested Candida albicans showed high susceptibility to the extract and growth was completely inhibited at a concentration of 400 microg/ml. At the same concentration, the acetone extract and the standard antifungal drug griseofulvin produced comparable zones of inhibition on C. albicans. Studies on the mode of action of the extract indicated that it was bactericidal and fungicidal. The antimicrobial activity of the extract was attributed to the high amount of hydrolysable tannins present in the bark of the plant.CONCLUSION:The acetone extract of the stem bark of C. molle has the potential for use as a natural antimicrobial agent. Further in vivo antimicrobial, phytochemical and toxicological studies are requireed to evaluate the chemotherapeutic effect of the plant." Antibacterial and antifungal activities of extracts of combretum molle. Asres K, Mazumder A, Bucar F. No
17489357 A new lignan glycoside was isolated from the stem bark of Styrax japonica (Styracaceae). This lignan glycoside, named styraxjaponoside C (1), was identified by spectroscopic methods. In addition, six known compounds, arctiin (2), pinoresinol-4-O-beta-D-glucopyranoside (3), matairesinoside (4), methylsyringin (5), syringin (6), and egonol (7) were isolated from this plant. The structures of 1-7 were determined on the basis of spectroscopic and physicochemical data. Compounds 2 and 5 were isolated from this plant for the first time. A new lignan glycoside from the stem bark of Styrax japonica S. et Z. Kim MR, Moon HT, Lee DG, Woo ER. No
17489633 Investigation of an organic extract of Schisandra rubriflora led to the isolation of three new highly oxygenated nortriterpenoids, named rubriflorins A-C (1-3), together with six related known compounds (4-9). Their structures were elucidated by spectroscopic evidence. Compounds 1-3 feature the opening of ring A compared with related known nortriterpenoids isolated from the genus Schisandra and showed anti-HIV-1 activities with EC50 values of 10.0, 16.2, and 81.3 microg/mL, respectively. Triterpenoids from Schisandra rubriflora. Xiao WL, Li XL, Wang RR, Yang LM, Li LM, Huang SX, Pu JX, Zheng YT, Li RT, Sun HD. No
17498888 Leaves, root and stem bark of Diospyros anisandra were screened against two strains of Mycobacterium tuberculosis, one resistant and one susceptible to antibiotics, using the microplate Alamar blue assay test. The lypophylic fractions of the root and bark showed significant inhibitory activity against both strains, with the hexane fraction of the bark showing the strongest activity (MIC 6.25 microg/ml) against the resistant strain and a significant antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Candida albicans, Aspergillus niger, and Colletotrichum gloeosporioides. The bioassay-guided purification of the bioactive hexane fraction resulted in the isolation and identification of the naphthoquinone plumbagin as one of the metabolites responsible for the biological activity. Antimicrobial activity of Diospyros anisandra. Borges-Argáez R, Canche-Chay CI, Peña-Rodríguez LM, Said-Fernández S, Molina-Salinas GM No
17511144 OBJECTIVE: To study the chemical constituents of the leaves of pineapple.METHOD: Chromatographic methods were used to isolate compounds from the leaves of pineapple and spectral methods were used to identify the structures of the isolated compounds.RESULT: Compound 1 was isolated from the leaves of pineapple. It was identified as 1-O-beta-D-glucopyranosyl-(2S, 3R, 4E, 11E)-2-[(2(R)-hydroxydocosanoyl) amido]-4, 11-hexadecanediene-1, 3-diol.CONCLUSION: Compound 1 was a new compound. [New cerebroside from leaves of pineapple]. Wang JP, Wang HY, Du LJ, Ding Y, Xing DM, Wang W. No
17511147 OBJECTIVE: To study the chemical constituents of Salsola collina.METHOD: The EtOH extract from the whole plant of S. collina were isolated and purified by column chromatography. These compounds were identified by their physical properties and spectroscopic data.RESULT: Eleven compounds were isolated and identified as ferulic acid (1), p-coumaric acid (2), salicylic acid (3), tricin (4), selagin (5), acanthoside D (6), tricin -7-O-beta-D-glucopyranoside (7), tricin-4'-O-beta-D-apioside (8), isorhamnetin-7-O-beta-D-glucopyranoside (9), isorhamnetin-3-O-beta-D-glucopyranoside (10), isorhamnetin-3-O-alpha-L-arabinopyranosyl (1-->6) -beta-D-glucopyranoside (11).CONCLUSION: wherein compound 1, 2, 3, 5, 6 and 9 were isolated from the Salsola for the first time. [Studies on chemical constituents of Salsola collina]. Xiang Y, Li YB, Zhang J, Li P, Yao YZ. No
17541202 Two new xanthones, 1,5,6-trihydroxy-6',6'-dimethyl-2H-pyrano(2',3':3,4)-2-(3-methylbut-2-enyl)xanthone (1) and 1,6,7-trihydroxy-6',6'-dimethyl-2H-pyrano(2',3':3,2)-4-(3-methylbut-2-enyl)xanthone (2), have been isolated from the stem bark of Garcinia lancilimba (Guttiferae), together with six known xanthones. Their structures were identified on the basis of extensive spectral evidence including detailed 2D NMR and HR-MS data. Two new compounds showed moderate inhibitory effect on human breast cancer MDA-MB-435S cell line. Two new xanthones isolated from the stem bark of Garcinia lancilimba. Yang NY, Han QB, Cao XW, Qiao CF, Song JZ, Chen SL, Yang DJ, Yiu H, Xu HX. No
17547068 This study reports the in vitro inhibitory potential of crude extract of Quercus lusitanica (Q. lusitanica) seeds on the replication of dengue virus type 2 (DEN-2). In vitro antiviral activity of Q. lusitanica extract, assessed in C6/36 cells (cloned cells of Aedes albopictus larvae) employing a virus inhibition assay, showed dose-dependent inhibition. The Q. lusitanica extract at its maximum non-toxic concentration of 0.25 mg/ml completely inhibited 10-1,000 TCID50 of virus, as indicated by the absence of cytopathic effect (CPE). The low dose of Q. lusitanica (0.032 mg/ml) showed 100% inhibition with 10 TCID50 of virus, but only 50% and 25% inhibition with 100 and 1,000 TCID50, respectively. The NS1 is a glycoprotein present in all flaviviruses and appears essential for virus viability. To further evaluate Q. lusitanica extract as an antiviral compound, we investigated the effect of Q. lusitanica extract on the NS1 protein expression of infected C6/36 cells through proteomics technique. The result showed downregulation of NS1 protein expression of infected C6/36 cells after treatment with this extract. In conclusion, we found that Q. lusitanica extract has a good inhibitory effect on the replication of dengue virus type 2, both in conventional cell culture and proteomics technique. Inhibitory potential of Quercus lusitanica extract on dengue virus type 2 replication Muliawan SY, Kit LS, Devi S, Hashim O, Yusof R. No
17552154 OBJECTIVE: To study the chemical constituents of Cynanchum forrestii.METHOD: Chromatographic techniques were applied to isolated chemical constituents. The structures were identified on the basis of physico-chemical constants and spectroscopic data.RESULT: Eight compounds were isolated from the 95% ethanol extract of the roots of C. forrestii and elucidated as ( + ) -5'-methoxyisolariciresinol 3a-O-beta-D-glucopyranoside (1), hexahydroxycholest-7-en-6-one (2), tylophorinidine (3), sucrose (4), palmitic acid (5), beta-sitosterol (6), daucosterol (7), nonanedioic acid (8).CONCLUSION: Compounds 1-3 from this genus, and compounds 4-8 from the plant were obtained for the first time. [Studies on chemical constituents of Cynanchum forrestii]. Liu Y, Liu J, Yu SS, Huang XZ, Hu YC. No
17552563 Bioactivity-directed fractionation of an extract of the leaves of Alvaradoa haitiensis, using the KB (human oral epidermoid carcinoma) cell line, led to the isolation and identification of 10 new anthracenone C-glycosides, alvaradoins E-N (1-10), along with the known compound chrysophanol (11). The cytotoxicity of all compounds was evaluated, and preliminary structure-activity relationships are suggested. The most potent compounds in the in vitro assays (1 and 2) were evaluated in vivo versus the P388 (murine lymphocytic leukemia) model, and alvaradoin E (1) showed antileukemic activity (125% T/C) at a dose of 0.2 mg kg-1 per injection when administered intraperitoneally. Alvaradoins E-N, antitumor and cytotoxic anthracenone C-glycosides from the leaves of Alvaradoa haitiensis. Phifer SS, Lee D, Seo EK, Kim NC, Graf TN, Kroll DJ, Navarro HA, Izydore RA, Jiménez F, Garcia R, Rose WC, Fairchild CR, Wild R, Soejarto DD, Farnsworth NR, Kinghorn AD, Oberlies NH, Wall ME, Wani MC. No
17571763 Active compounds from Actinidia deliciosa were separated and identified. Column chromatography was used for isolation. Physicochemical and spectroscopic analysis were employed for structural identification. 5 compounds were isolated and identified as 2alpha, 3beta, 19, 23-tetrahydroxylolean-12-en-28-oic acid( I ), 2alpha, 3alpha, 23-trihydroxy-urs-12-en-28-oic acid( II ) , 2alpha, 3beta, 19,23-tetra-hydroxyl-urs-12-en-oic acid( I ) , undecanoic acid( IV) ,beta-sitoserol( V ). I , IV and V were isolated from roots of Actinidia deliciosa for the first time. [Study on the chemical structure from the roots of Actinidia deliciosa]. Lai Y, Xu DP. No
17571764 OBJECTIVE: To study the chemical constituents of the flower of Paulownia fortunei (Seem.) Hemsl.METHODS: The constituents were isolated by column chromatography and their structures were elucidated through spectroscopic analysis.RESULTS: The compounds were identified as diplacone( I ) ,3 '-O-methyldiplacone ( II ) , mimulone ( ll) , ursolic acid( IV ), beta-sitosterol ( V ), daucosterol ( VI).CONCLUSION: All these compounds were obtained from the flower for the first time. [Chemical constituents from the flower of Paulownia fortunei( seem. ) Hemsl]. Duan WD, Zhang J, Xie G, Zhang CZ, Li C. No
17582701 Crude methanol and water extracts of 36 plants, employed in the treatment of diseases of probable bacterial etiology by the Venda people, were screened for antibacterial activity. Combretum molle, Peltophorum africanum, Piper capense, Terminalia sericea and Zanthoxylum davyi were the most active and presented MIC values < or =1.00 mg/ml. Antibacterial activity of Venda medicinal plants. Steenkamp V, Fernandes AC, van Rensburg CE. No
17583201 OBJECTIVE: To investigate the chemical constituents from flowers of Sesamum indicum.METHOD: Column chromatography with silica gel, C18 and Sephadex LH -20 as packing materials was used to separate the chemical constituents, and the structures were determined by chemical and spectroscopic methods.RESULT: Six flavones were isolated and elucidated as apigenin (1), ladanetin (2), ladanetin-6-O-beta-D-glucoside (3), apigenin-7-O-glucuronic acid (4), pedalitin (5), and pedalitin-6-O-glucoside (6).CONCLUSION: All of the compounds were isolated from this plant for the first time. [Flavones from flowers of Sesamum indicum]. Hu YM, Wang H, Ye WC, Zhao SX. No
17583202 OBJECTIVE: To study the chemical constituents of Serissa serissoides.METHOD: Chemical constituents were isolated with the column chromatographic methods including silica gel and sephadex LH -20, and their structures were elucidated on the basis of their spectral and chemical evidences.RESULT: Eight compounds were obtained and were identified as: palmitic acid (1), corosolic acid (2), daucosterol (3), urs-12-en-28-ol (4), oleanolic acid (5), uroslic acid (6), 4-hydroxy-3-methoxybenzoic acid (7), and 2,6-dimethoxy-p-benzoquinone (8).CONCLUSION: Except compound 5, other seven compounds were found from the genus Serissa for the first time. [Studies on chemical constituents from Serissa serissoides]. Li YL, Wang G, Xue JY, Cen YZ. No
17583499 Mosquitoes are important vectors of diseases and nuisance pests. Repellents minimize contact with mosquitoes. Repellents based on essential oils (EO) are being developed as an alternative to DEET (N,N-diethyl-m-methylbenzamide), an effective compound that has disadvantages including toxic reactions, and damage to plastic and synthetic fabric. This work evaluated the repellency against Aedes aegypti of EO from aromatic plants that grow in Argentina: Acantholippia seriphioides, Achyrocline satureioides, Aloysia citriodora, Anemia tomentosa, Baccharis spartioides, Chenopodium ambrosioides, Eucalyptus saligna, Hyptis mutabilis, Minthostachys mollis, Rosmarinus officinalis, Tagetes minuta and Tagetes pusilla. Most EO were effective. Variations depending on geographic origin of the plant were detected. At a 90% EO concentration, A. satureoides and T. pusilla were the least repellent. At concentrations of 12.5% B. spartioides, R. officinalis and A. citriodora showed the longest repellency times. Comparisons of the principal components of each EO suggest that limonene and camphor were the main components responsible for the repellent effects. Mosquito repellent activity of essential oils of aromatic plants growing in Argentina. Gillij YG, Gleiser RM, Zygadlo JA. No
17603210 A new C(19)-diterpenoid alkaloid, ouvrardiantine (1) and two new C(20)-diterpenoid alkaloids, ouvrardiandines A (2) and B (3) were isolated from the root of Aconitum ouvrardianum HAND.-MAZZ. The structure of the new alkaloids was established on the basis of spectral data (1D- and 2D-NMR, HR-MS). Three new diterpenoid alkaloids from roots of Aconitum ouvrardianum HAND.-MAZZ. Hou LH, Chen DL, Jian XX, Wang FP. No
17608220 OBJECTIVE: To study the chemical constituents in the root of Isatis indigotica.METHOD: The constituents root were separated through various chromatographic techniques and their structures were elucidated by means of physicochemical properties and the analysis of their spectral data.RESULT: Eleven compounds were isolated and identified as (+) -isolariciresinol (1), lariciresinol (2), lariciresinol-9-O-beta-D-glucopyranoside (3), lariciresinol-4'-O-beta-D-glucopyranoside (4), lariciresinol-4,4'-bis-O-beta-D-glucopyranoside (5), 3-formylindole (6), 1-methoxy-3-indolecarbaldehyde (7), 1-methoxy-3-indoleacetonitrile (8), deoxyvasicinone (9), epigoitrin (10), adenosine (11).CONCLUSION: Compounds 4-8 were isolated from I. indigotica for the first time. [Studies on chemical constituents in root of Isatis indigotica]. Zuo L, Li JB, Xu J, Yang JZ, Zhang DM, Tong YL. No
17608221 OBJECTIVE: To study the chemical constituents from the stems and leaves of Calophyllum inophyllum.METHOD: The compounds were isolated by column chromatography on silica gel, Sephadex LH-20 and preparative TLC. Their structures were elucidated by chemical methods and NMR, MS spectroscopic data.RESULT: Nine compounds were identified as 2-hydroxyxanthone (1), 4-hydroxyxanthone (2), 1, 5-dihydroxyxanthone (3), 1, 7-dihydroxyxanthone (4), 1, 3, 5-trihydroxy-2-methoxyxanthone (5), 6-deoxyjacareubin (6), amentoflavone (7), kaempferol-3-O-alpha-L-rhamnoside (8) and quercetin-3-O-alpha-L-rhamnoside (9).CONCLUSION: Compounds 8 and 9 were isolated from the genus Calophyllum and compounds 1, 2, 4-6 were isolated from this plant for the first time. [Studies on flavonoids from stems and leaves of Calophyllum inophyllum]. Li YZ, Li ZL, Hua HM, Li ZG, Liu MS. No
17608222 OBJECTIVE: To study the chemical constituents from the leaves of Morus multicaulis.METHOD: The compounds were isolated by ion exchange resin, silica gel and Sephadex LH -20 column chromatographies. Their structures were elucidated on the basis of physico-chemical properties and spectral data.RESULT: Twelve compounds were isolated from the leaves of this plant. Their structures were identified as 1-deoxynojirimycin (1), fagomine (2), 2-O-alpha-D-galactopyranosyl-1-deoxynojirimycin (3), quercetin-7-O-beta-D-glucoside (4), kaempferol (5), quercetin (6), scopoletin (7), D-aspartic acid (8), L-proline (9), D-alpha-alanine (10), myo-inositol (11) and dausterol (12).CONCLUSION: All compounds were isolated from this plant for the first time. [Chemical constituents from leaves of Morus multicaulis]. Han WL, Liu L, Zhang XQ, Ye WC, Pan YL, Yao XS. No
17613618 A new compound, kaempferol 3-O-(2''-O-galloylrutinoside) (1), was isolated from the white flower of Nymphaea candida, together with nine known flavonol glycosides, kaempferol (2), kaempferol 3-O-beta-D-glucopyranoside (3), kaempferol 3-O-alpha-l-rhamnopyranoside (4), kaempferol 3-O-alpha-l-rhamnopyranosylglucopyranoside (5), kaempferol 7-O-beta-D-glucopyranoside 3-(O-alpha-l-rhamnopyranosylglucopyranoside) (6), quercetin (7), quercetin 3-O-beta-D-xylopyranoside (8), myricetin (9), myricetin 3'-O-beta-D-xylopyranoside (10). The structure of 1 was established on the basis of the analysis of its 1D and 2D NMR spectral data. Compounds 1-7 and 9 exhibited moderate to significant antioxidant activities, which were evaluated by measurement of low-density lipoprotein (LDL) and malondialdehyde (MDA) levels in vitro. Compounds 1, 3, 4, 6 and 9 exhibited promising neuroprotective effects on ischemic injury model of cultured rat cortical neurons treated with sodium dithionite in glucose-free medium. Furthermore, compounds 1, 5, and 9 had distinct cytotoxicity to adrenal gland pheochromocytoma, PC12 cells, being treated by the same way. A new flavonol glycoside and activity of compounds from the flower of Nymphaea candida. Liu RN, Wang W, Ding Y, Xie WD, Ma C, Du LJ. No
17613626 Chemical investigation on the roots of Cudrania fruticosa resulted in the isolation of two new xanthones, 1,6,7-trihydroxy-2-(1,1-dimethyl-2-propenyl)-3-methoxyxanthone (1) and 3,6,7-trihydroxy-1-methoxyxanthone (2), together with three known ones, 1,3,5-trihydroxy-4-(3-hydroxy-3-methylbutyl)xanthone (3), 1,3-dihydroxy-6,7-dimethoxyxanthone (4) and 3,5,6-trihydroxy-1-methoxyxanthone (5), respectively. Their structures were elucidated on the basis of spectral and chemical techniques. Xanthones from the roots of Cudrania fruticosa Wight. Liang B, Li HR, Xu LZ, Yang SL. No
17616904 One new pentacyclic triterpenoid psidiumoic acid (5) along with four known compounds beta-sitosterol (1), obtusol (2), oleanolic acid (3), and ursolic acid (4) have been isolated from the leaves of Psidium guajava. The new constituent 5 has been characterized as 2 alpha-glycolyl-3beta-hydroxyolean-12-en-28-oic acid through 2D NMR techniques. This is the first report of isolation of compound 2 from the genus Psidium. A new ethylene glycol triterpenoid from the leaves of Psidium guajava. Begum S, Ali SN, Hassan SI, Siddiqui BS. No
17639983 OBJECTIVE: To isolate and elucidate the constituents of herbs of Polygala hongkongensis.METHOD: The constituents were isolated and purify by chromatographic on silica gel, Sephadax LH-20 and semi-preparative HPLC. The structures were determined by NMR and MS spectral analysis.RESULT: Six compounds were identified as euxanthone (1), 1, 3, 6-trihydroxyxanthone (2), 1, 4, 7-trihydroxy-3-methoxy-xanthone (3), p-hydroxybenzoic acid (4), 3, 4-dihydroxybenzoic acid (5), and methy 1 2, 5-dihydroxybenzoate (6).CONCLUSION: The known compounds 1-6 were isolated from this plant for the first time. [Isolation and structure identification of chemical constituents from Polygala hongkongensis II]. Wu JF, Chen SB, Wu LJ, Chen SL, Tu PF. No
17639985 OBJECTIVE: To study the chemical constituents of Eriophyton wallichii.METHOD: The constituents were isolated and purified by silica gel column and Sephadex LH-20 chromatography. Their structures are elucidated by physicochemical properties and spectral analysis.RESULT: Seven compounds were obtained and identified as beta-sitosterol (1), marrubiin (2), ursolic acid (3), cimigoside (4), 5-deoxyantirrhinoside (5), 8-epiloganic acid (6) and apigenin 7-(6"-p-coumaroyl) glucoside (7).CONCLUSION: Compounds 1-7 were isolated from this plant for the first time. [Studies on chemical constituents of Eriophyton wallichii]. Zhang S, Geshang SL, Dawa ZM, Zhou Y, Peng SL. No
17655148 OBJECTIVE: To study the chemical constituents of Taraxacum mongolicum.METHOD: Repeated column chromatography over silica gel, C-18 and Sephadex LH-20 were used to isolate chemical constitutes and their structures were elucidated by spectroscopic methods MS, 1H-NMR, 13C-NMR including 2D-NMR techniques and by direct comparing spectral data with those reported in literatures.RESULT: Eight compounds were identified as artemetin (1), quercetin (2), luteolin (3), luteolin-7-O-beta-D-glucopyranoside (4), caffeic acid (5), esculetin (6), stigmasterol (7), taraxasteryl acetate (8), respectively.CONCLUSION: Compound 1 was obtained from this genus and compound 6, 8 from this species for the first time. Furthermore, the 13 carbon data of compound 6 from the literature were corrected with 2D-NMR techniques in this paper. [Studies on constitutes from Taraxacum mongolicum]. Yao W, Lin WY, Zhou CX, Zhao Y. No
17655149 OBJECTIVE: To study the aqueous constituents of Houttuynia cordata.METHOD: Various columns including Diaion HP-20, Sephadex LH-20, ODS and silica gel were employed for the isolation and purification of compounds from H. cordata. The structures of the compounds were identified by physiochemical properties and spectral analysis.RESULT: Five compounds were isolated, and their structures were identified as chlorogenic methyl ester (1), (E)-4-Hydroxy-4-[3'-(beta-D-glucopyranosyloxy) butylidene]-3, 5, 5-trimethyl-2-cyclohexen-1-one (2), 2-(3, 4-dihydroxyphenyl) ethyl-beta-D-glucopyranoside (3), p-hydroxyphenethyl-beta-D-glucoside (4), 4-(beta-D-glucopyranosyloxy)-3-hydroxy-Benzoic acid (5).CONCLUSION: All compounds were isolated from this plant for the first time. [Studies on chemical constituents of phenols in fresh Houttuynia cordata]. Meng J, Dong XP, Zhou YS, Jiang ZH, Leung SY, Zhao ZZ. No
17718198 Four new furostanol saponins, named as macrostemonoside O, macrostemonoside P, macrostemonoside Q and macrostemonoside R, along with five known compounds, were isolated from the dried bulbs of Allium macrostemon Bunge. The structures of these new compounds were established by the spectral data elucidation (IR, ESIMS, 1D and 2D NMR) as 26-O-beta-D-glucopyranosyl-22-hydroxyl-5beta-furost-25 (27)-ene-3beta, 26-diol-3-O-beta-D-glucopyranosyl (1-->2)-beta-D-galactopyranoside (macrostemonoside O), (25R)-26-O-beta-D-glucopyranosyl-22-hydroxyl-5beta-furost-1beta, 3beta, 26-triol-3- O-beta-D-glucopyranosyl (1-->2)-beta-D-galactopyranoside (macrostemonoside P), (25R)-26-O-beta-D-glucopyranosyl-22-hydroxy-5beta-furost-1alpha, 2beta, 3beta, 26-tetraol-3-O-beta-D-glucopyranosyl (1-->2)-beta-D-galactopyranoside (macrostemonoside Q) and (25R)-26-O-beta-D-glucopyranosyl-22-hydroxyl-5beta-furost-2alpha, 3beta, 26-triol-3-O-beta-D-glucopyranosyl (1-->2) [beta-D-glucopyranosyl (1-->3)]-beta-D-glucopyranosyl (1-->4)-beta-D-galactopyranoside (macrostemonoside R), respectively. Their cytotoxic activities on several cancer cell lines including solid tumor (HepG2, MCF-7, NCI-H460 and SF-268) and drug resistant tumor (R-HepG2) were investigated and five compounds showed diverse cytotoxity to these cancer cell lines which suggest that they might be used as potential leading compounds to cure cancer diseases. New furostanol saponins from the bulbs of Allium macrostemon Bunge and their cytotoxic activity. Chen H1, Wang G, Wang N, Yang M, Wang Z, Wang X, Yao X. No
17720339 Phyllanthus amarus Schum. et Thonn. (Bhuia amla; Euphorbiacae) is a herb common to central and southern India. It is an ayurvedic herb and has a wide range of traditional uses in different diseases. The aim of this work was to evaluate the hepatoprotective effect of ethanolic extract of Phyllanthus amarus (Phyllanthus amarus) on aflatoxin B(1)-induced liver damage in mice using different biochemical parameters and histopathological studies. Aflatoxin was administered orally (66.6 microg kg(-1)BW 0.2 ml(-1)day(-1)) to the mice of each group except control to which normal saline and ascorbic acid (0.1g kg(-1)BW 0.2 ml(-1)day(-1)) were given, respectively. Ethanolic extract of Phyllanthus amarus (0.3g kg(-1)BW 0.2 ml(-1)day(-1)) was given to all groups except control groups (gp. I and gp. V) after 30 min of aflatoxin administration. The entire study was carried out for 3 months and animals were sacrificed after an interval of 30 days till the completion of study. Phyllanthus amarus extract was found to show hepatoprotective effect by lowering down the content of thiobarbituric acid reactive substances (TBARS) and enhancing the reduced glutathione level and the activities of antioxidant enzymes, glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT). Histopathological analyses of liver samples also confirmed the hepatoprotective value and antioxidant activity of the ethanolic extract of the herb, which was comparable to the standard antioxidant, ascorbic acid. The overall data indicated that Phyllanthus amarus possesses a potent protective effect against aflatoxin B(1)-induced hepatic damage, and the main mechanism involved in the protection could be associated with its strong capability to reduce the intracellular level of reactive oxygen species by enhancing the level of both enzymatic and non-enzymatic antioxidants. Hepatoprotective effect of ethanolic extract of Phyllanthus amarus Schum. et Thonn. on aflatoxin B1-induced liver damage in mice. Naaz F, Javed S, Abdin MZ. No
17723297 Two new alkaloids, hypserpanines A and B (1, 11), together with eleven known compounds, phenolbetain (2), acutumine (3), acutumidine (4), dechloroacutumine (5), dauricumine (6), dauricumidine (7), pronuciferine (8), glaziovine (9), S-reticuline (10), magnoflorine (12) and laurifoline(13), were isolated from Hypserpa nitida Miers. (Menispermaceae) and chemically elucidated through spectral analyses. All the isolated alkaloids were evaluated for their anti-HBV activities in vitro using the HBV transfected Hep G2.2.15 cell line. The most active compound, dauricumidine (7), exhibited an IC(50) value of 0.450 mM (SI=4.13) on hepatitis B virus (HBV) surface antigen (HBsAg) secretion of the Hep G2.2.15 cell line Two new alkaloids and active anti-hepatitis B virus constituents from Hypserpa nitida. Cheng P, Ma YB, Yao SY, Zhang Q, Wang EJ, Yan MH, Zhang XM, Zhang FX, Chen JJ. No
17727058 OBJECTIVE: To isolate and identify the chemical constituents from the whole plant of Pyrethrum tatsienense, which has been used as traditonal herb medicine in the treatment of some diseases.METHODS: The compounds were isolated by column chromatography and their structures were elucidated through spectroscopic analysis (NMR).RESULTS: Six compounds were isolated and identified as: linoeic acid, pinoresinol, (+)-pinoresinol-4'-O-beta-D- glucopyranoside, betulabuside A, dihydrosyringin.CONCLUSION: All these compounds were obtained from Pyrethrum tatsienense for the first time. [Chemical constituents from Pyrethrum tatsienense]. Yang AM, Lu RH, Shi YP. No
17727061 OBJECTIVE: To investigate the chemical constituents of the aerial parts of Plumbago zeylanica Linn.METHODS: The constituents of the EtOAc-soluble portion in the 95% ethanol extract were isolated and purified by means of chromatography. Compounds were identified by their physical characteristics and spectral features.RESULTS: Nine compounds were isolated as plumbagin (I), isoshinanolone (II), plumbagic acid (III), beta-sitosterol (IV), 4-hydroxybenzaldehyde (V), trans-cinnamic acid (VI), vanillic acid (VII), 2, 5-dimethyl-7-hydroxychromone (VIII), indole-3-carboxaldehyde (IX).CONCLUSION: Compounds V, VII, VIII and IX were isolated for the first time from Plumbago Linn. [Chemical constituents from aerial parts of Plumbago zeylanica Linn]. Zhang QR, Mei ZN, Yang GZ, Xiao YX. No
17845001 A new dimeric aporphine alkaloid, bidebiline E (1), and a new natural product, octadeca-9,11,13-triynoic acid (2), along with three known sesquiterpenes, alpha-humulene (3), caryophyllene oxide (4), and (-)-alpha-cadinol (5), and four known isoquinoline alkaloids, laudanosine (6), codamine (7), laudanidine (8), and reticuline (9), were isolated from the roots of Polyalthia cerasoides. The structures of compounds 1 and 2 were established on the basis of their 1D and 2D NMR spectroscopic data. Among these isolates, 1, 2, 4, 7, and 8 exhibited antimalarial activity against Plasmodium falciparum, while 1- 3 showed antimycobacterial activity against Mycobacterium tuberculosis using in vitro assays. Bioactive constituents of the roots of Polyalthia cerasoides. Kanokmedhakul S1, Kanokmedhakul K, Lekphrom R. No
17851394 Four new diterpenoid alkaloids: tiantaishansine (1), tiantaishannine (2), tiantaishanmine (3), and tiantaishandine (4) have been isolated from the roots of Delphinium tiantaishan. Their structures were elucidated by chemical evidence and spectral analyses, including ESI-MS, HR-EI-MS, 1D- and 2D-NMR. New diterpenoid alkaloids from the roots of Delphinium tiantaishanense. Li J, Chen DL, Jian XX, Wang FP. No
17852738 Two highly oxygenated hetisine-type diterpenoid alkaloids, delphigraciline (1), 14-hydroxyhetisinone N-oxide (2), and the norditerpenoid alkaloid 8-methoxykarakoline (3), were isolated from a neutral extract of Delphinium gracile. Their structures were elucidated on the basis of spectroscopic data and by comparison with previously reported spectroscopic data of similar alkaloids. Their antiparasitic and insecticidal activities are also discussed. Diterpenoid alkaloids from Delphinium gracile. Reina M, Mancha R, Gonzalez-Coloma A, Bailen M, Rodriguez ML, Martinez-Diaz RA. No
17879729 OBJECTIVE: To investigate the chemical constituents of the rhizome of Ervatamia hainanensis.METHOD: The solvent extraction and silica column chromatography were used to separate the chemical constituents, and their structures were identified by physico chemical properties and spectra analysis.RESULT: Twelve compounds were isolated and their structures were identified as voacangine (1), ibogaine (2), ibogamine (3), coronaridine (4), 19-heyneanine (5), 19-epi-heyneanine (6), 3-hydroxyl coronaridine (7), coronaridine hydroxyindolenine (8), 3-(2-oxopropyl) coronaridine (9), vobasine (10), alpha-amyrin (11), alpha-amyrin acetate (12).CONCLUSION: Compounds 1, 2, 6, 11 and 12 were first found from this plant. [Study on chemical constituents in rhigome of Ervatamia hainanensis]. Liang S, Chen HS, Jin YS, Jin L, Lu J, Du JL. No
17885833 Two new neolignan glycosides, davidioside A (1) and davidioside B (2), have been isolated along with six known compounds from the branch bark of Davidia involucrata. Identification of their structures was achieved by 1D and 2D NMR experiments, including (1)H-(1)H COSY, NOESY, HMQC, and HMBC methods as well as FAB mass spectral data. Neolignan and lignan glycosides from branch bark of Davidia involucrata. Ouyang MA, Huang J. No
17885837 Two new compounds, alternanthin B (1) and N-trans-feruloyl-3,5-dimethoxytyramine (2), along with four known compounds (3-6) were isolated from the aerial parts of Alternanthera philoxeroides. Their structures were elucidated on the basis of spectroscopic methods. The antitumor activity of the isolated compounds was also evaluated. Antitumor constituents from Alternanthera philoxeroides. Fang JB, Jia W, Gao WY, Yao Z, Teng J, Zhao AH, Duan HQ. No
17885838 Two new pregnane saponins, solanigroside A (1) and solanigroside B (2), along with two known compounds (3 and 4), were isolated from 60% ethanolic extract of the dried herb of Solanum nigrum L. The structures of 1 and 2 were elucidated as 5alpha-pregn-16-en-3beta-ol-20-one 3-O-beta-D-xylopyranosyl-(1 --> 3)-O-[alpha-L-arabinopyranosyl-(1 --> 2)]-O-beta-D-glucopyranosyl-(1 --> 4)-O-[alpha-L-rhamnopyranosyl-(1 --> 2)]-O-beta-D-galactopyranoside (1) and 5alpha-pregn-16-en-3beta-ol-20-one 3-O-beta-D-glucopyranosyl-(1 --> 2)-O-[beta-D-glucopyranosyl-(1 --> 3)]-O-beta-D-glucopyranosyl-(1 --> 4)-O-beta-D-galactopyranoside (2), respectively, on the basis of extensive spectroscopic analysis as well as comparison with reported spectroscopic data of related compounds. This paper deals with the isolation and structural characterisation of pregnane glycosides from S. nigrum L. Pregnane glycosides from Solanum nigrum. Zhou XL, He XJ, Zhou GX, Ye WC, Yao XS. No
17887722 Four new Daphniphyllum alkaloids, daphnipaxianines A-D ( 1- 4), along with six known ones, have been isolated from the leaves and fruits of Daphniphyllum paxianum. Daphnipaxianines A and B ( 1, 2), a pair of epimers differing at C-10, are the first caliciphylline A type Daphniphyllum alkaloids with a Delta (9(15))-unsaturated cyclic ketone unit, and daphnipaxianine D ( 4) is the first yuzurine-type Daphniphyllum alkaloid containing a hexacyclic ring system. The structures of these alkaloids were characterized by spectroscopic methods, especially 2D NMR techniques. A single-crystal X-ray diffraction analysis was used to confirm the structure of 1. Daphnipaxianines A-D, alkaloids from Daphniphyllum paxianum. Mu SZ1, Li CS, He HP, Di YT, Wang Y, Wang YH, Zhang Z, Lü Y, Zhang L, Hao XJ. No
17894898 Abstarct:"BACKGROUND:New prophylactic and therapeutic tools are needed for the treatment of herpes simplex virus infections. Several essential oils have shown to possess antiviral activity in vitro against a wide spectrum of viruses.AIM:The present study was assess to investigate the activities of the essential oil obtained from leaves of Artemisia arborescens against HSV-1 and HSV-2METHODS:The cytotoxicity in Vero cells was evaluated by the MTT reduction method. The IC50 values were determined by plaque reduction assay. In order to characterize the mechanism of action, yield reduction assay, inhibition of plaque development assay, attachment assay, penetration assay and post-attachment virus neutralization assay were also performed.RESULTS:The IC50 values, determined by plaque reduction assay, were 2.4 and 4.1 microg/ml for HSV-1 and HSV-2, respectively, while the cytotoxicity assay against Vero cells, as determined by the MTT reduction method, showed a CC50 value of 132 mug/ml, indicating a CC50/IC50 ratio of 55 for HSV-1 and 32.2 for HSV-2. The antiviral activity of A. arborescens essential oil is principally due to direct virucidal effects. A poor activity determined by yield reduction assay was observed against HSV-1 at higher concentrations when added to cultures of infected cells. No inhibition was observed by attachment assay, penetration assay and post-attachment virus neutralization assay. Furthermore, inhibition of plaque development assay showed that A. arborescens essential oil inhibits the lateral diffusion of both HSV-1 and HSV-2.CONCLUSION:This study demonstrates the antiviral activity of the essential oil in toto obtained from A. arborescens against HSV-1 and HSV-2. The mode of action of the essential oil as antiherpesvirus agent seems to be particularly interesting in consideration of its ability to inactivate the virus and to inhibit the cell-to-cell virus diffusion." Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells. Saddi M, Sanna A, Cottiglia F, Chisu L, Casu L, Bonsignore L, De Logu A. PMC2099429
17918436 OBJECTIVE: To study the chemical constituents of Buddleja purdomii W. W. Smith.METHODS: The constituents were isolated and purified by various chromatographic methods and structurally identified by spectral analysis.RESULTS: 7 compounds were obtained as luteolin (I), luteolin-7-O-beta-D-glucoside (II), trans-caffeic acid (III), cis-caffeic acid (IV), beta-stiosterol (V), stigmasterol (VI), nonacosane (VII).CONCLUSION: All these compounds are obtained from this plant for the first time. [Study on the terpenoids of chemical constituents of Buddleja purdomii (II)]. Gao Y, Cai L, Li HY, Li C. No
17918437 OBJECTIVE: To investigate the chemical constituents from the branches and leaves of Cupressus duolouxiana native to China in order to obtain a more comprehensive understanding of its effective components.METHODS: Compounds were isolated and purified by silica gel column chromatography. They were identified and structurally elucidated by spectral analysis.RESULTS: Six compounds were isolated from the ethanolic extracts of Cupressus duolouxiana, which were identified as (7S,8S)-3-methoxy-3',7-epoxy-8,4'-oxy-neoligna-4,9,9'-triol (I), kaempferol (II), quercetin (III), juglanin (IV), quercetin-3'-methoxy-3-O-alpha-L-arabinofuranoside (V) and avicularin (VI).CONCLUSION: All compounds are obtained from this plant for the first time. [Studies on the chemical constituents from the branches and leaves of Cupressus duolouxiana]. Xu JF, Tan NH. No
17944186 Abstarct:"OBJECTIVE:Chemical constituents of the non-saponins from Solonum nigrum L. were studied.METHODS:The separation was performed on the silic gel CC, Sephadex-LH20 CC as well as preparative HPLC. The constituents were isolated and identified by spectral methods.RESULTS:Five compounds isolated from 60% ethanol extract werre identified as 6-methoy-hydroxycoumarin (I), syringaresinol-4-O-beta-D-glucopyranoside (II), pinoresinol-4-O-beta-D-glucopyranoside (III), 3, 4-dihydroxhbenzoic acid (IV), p-hydroxybenzoic acid (V), 3-methoxy-4-hydroxyienzoic acid (VI), adenosine (VII).CONCLUSION:II, III, and VII were isolated from this genus for the first time." [Non-saponins from Solanum nigrum L]. Wang LY, Wang NL, Yao XS. No
17944188 BJECTIVE: To study the chemical constituents in the whole herb of Anoectochiluts roxburghii (Wall) Lindl.METHOD: The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by IR, NMR and MS evidences.RESULT: Fourteen compounds were obtained and there into ten were identified as beta-sitosterol(2), ferulic acid(3), oleanolic acid(6), lanosterol(7), p-hydroxybenzaldehyde(8), 3-methoxyl-p-hydroxybenzaldehlde(9), daucosterol(10),3',4',7-trimethoxy-3, 5-dihydroxyflavone(11), isorhamnetin-3-O-beta-D-rutinoside(12) and rutin(13).CONCLUSION: Compounds 6,7,9 and 11 from genera Anoeetochilus were isolated for the first time. [Studies on the chemical constituents from herba anoectochili]. Yang XW, Han MH, Jin YP. No
17966354 OBJECTIVE: To investigate the chemical constituents of Helwingia Chinensis.METHOD: Compounds were isolated with silica gel, Sephadex LH - 20 and polyamide chromatography, and their structures were elucidated by means of spectral analysis.RESULT: Six compounds were isolated and identified as cinnamic acid (1), gult-5-en-3beta-ol (2), friedelin (3), alpha-amyrin (4), luteolin-7-O-beta-D-glucoside (5) and 4, 5-dimethoxy-1, 2-benzoquinone (6).CONCLUSION: All these Compounds were obtained from H. chinensis for the first time. [Studies on chemical constituents of Helwingia chinensis]. Yang J, Lou FM, Niu YK. No
17970593 Four new triterpenoids, kadlongilactones C-F (2-5), containing a consecutive hexacyclic [7,7,5,6,6,6] ring system, were isolated from the leaves and stems of Kadsura longipedunculata. In comparison with the NMR data of kadlongilactones A (1) and D (3), a significant phenomena was discovered that ring D of 3 inverted from a half-chair in 1 to a half-boat conformation when the HO-16 group changed from alpha- to beta-orientation. The structures of 2-5 were established on the basis of detailed spectroscopic analysis, and DFT computational methods were applied in the structural validation of compounds 3 and 5. Compounds 1-4 showed significant cytotoxicity against A549, HT-29, and K562 cell lines with IC50 values of 0.49-3.61 microM in vitro. Isolation and structure elucidation of kadlongilactones C-F from Kadsura longipedunculata by NMR spectroscopy and DFT computational methods. Pu JX, Huang SX, Ren J, Xiao WL, Li LM, Li RT, Li LB, Liao TG, Lou LG, Zhu HJ, Sun HD. No
17972581 OBJECTIVE: To study chemical constitutents of the rhizomes of Ligusticum chuanxiong.METHOD: The rhizomes of L. chuanxiong were extracted with water to afford a water extract which was participated between H2O and EtOAc, n-BuOH, successively. The compounds were isolatedand purified by column chromatography from the EtOAc fraction, and identified based on spectral analyses.RESULT: Eleven compounds were isolated from the rhizomes of L. chuanxiong, and structures were characterized as (4S)-p-menth-1-ene4, 7-diol (1), aromadendrane-4alpha, 10beta-diol (2), aromadendrane-4beta, 10alpha-diol (3), aromadendrane-4beta, 10beta-diol (4), augustic acid (5), xiongterpene (6), pregnenolone (7), 3(R), 8(S), 9(Z)-falcarindiol (8), senkyunolide F (9), senkyunolide I (10), 4-hydroxyl-3-butylphthalide (11).CONCLUSION: Compounds 1-5 were obtained from the rhizomes of L. chuanxiong for the first time. [Studies on chemical constituents of rhizomes of Ligusticum chuanxiong]. Chang XL, Ma YB, Zhang XM, Jiang ZY, Chen JJ. No
17985919 Guajadial (1), a novel caryophyllene-based meroterpenoid, was isolated from the Leaves of Psidium guajava (guava). The structure and relative stereochemistry of guajadial (1) were elucidated by extensive spectroscopic analysis. A possible biosynthetic pathway for 1 was proposed. Guajadial: an unusual meroterpenoid from guava leaves Psidium guajava. Yang XL, Hsieh KL, Liu JK. No
17994391 Fourteen prenylated xanthone derivatives were isolated from gamboge, the dry latex of Garcinia hanburyi, and their structures were elucidated by a detailed spectroscopic analysis. Five of them, isogambogenic acid (1), desoxymorellinin (2), 10-methoxygambogenic acid (3), 10-methoxygambogic acid (4) and 10-ethoxy gambogic acid (5), are new compounds. All of them showed potent cytotoxicity against HL-60, SMMC-7721 and BGC-83 cells. Five novel prenylated xanthones from Resina Garciniae. Feng F, Liu WY, Chen YS, Guo QL, You QD. No
18020421 Three new clerodane diterpene glycosides, tinosposinensides A-C ( 1- 3), and a new rearranged cadinane sesquiterpene glycoside, tinosinenside ( 4), were isolated from the stems of Tinospora sinensis. The structure including the relative configuration was elucidated on the basis of spectroscopic analysis. The absolute configuration was determined by application of the modified Mosher's method and chemical transformation. The inhibitory activities of the isolated compounds against alpha-glucosidase are also described. Structure and absolute configuration of clerodane diterpene glycosides and a rearranged cadinane sesquiterpene glycoside from the stems of Tinospora sinensis. Li W1, Wei K, Fu H, Koike K. No
18022654 Three xanthones, polyanxanthone A (1), B (2) and C (3) have been isolated from the methanol extract of the wood trunk of Garcinia polyantha, along with five known xanthones: 1,3,5-trihydroxyxanthone (4); 1,5-dihydroxyxanthone (5); 1,3,6,7-tetrahydroxyxanthone (6); 1,6-dihydroxy-5-methoxyxanthone (7) and 1,3,5,6-tetrahydroxyxanthone (8). Their structures were determined by means of 1D- and 2D-NMR techniques. Some of the above compounds were screened for their anticholinesterase activity on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. Polyanxanthone A, B and C, three xanthones from the wood trunk of Garcinia polyantha Oliv. Louh GN, Lannang AM, Mbazoa CD, Tangmouo JG, Komguem J, Castilho P, Ngninzeko FN, Qamar N, Lontsi D, Choudhary MI, Sondengam BL. No
18027663 OBJECTIVE: To study the chemical constituents from the root of Actinidia chinensis. METHOD: The roots of A. chinensis were extracted with 95% EtOH. The EtOH extract was suspended in H2O and extracted with petroleum ether, EtOAc and BuOH successively. The compounds were isolated with column chromatography from the EtOAc fraction, and elucidated on the basis of spectral analysis(MS, IR, 1H-NMR, 13C-NMR). RESULT: Seven compounds were isolated from the root of A. chinensis, and the structures were identified as 2alpha-hydroxyoleanolic acid (1), 2alpha-hydroxyursolic acid (2), euscaphic acid (3), 23-hydroxyursolic acid (4), 3beta-O-acetylursolic acid (5), ergosta4, 6, 8, (14), 22-tetraen-3-one (6), beta-steriol (7). CONCLUSION: All the compounds were obtained from the root of A. chinensis for the first time. [Chemical constituents from root of Actinidia chinensis]. Cui Y, Zhang XM, Chen JJ, Zhang Y, Lin XK, Zhou L. No
18051898 OBJECTIVE: To investigate the chemical constituents of Ilex pernyi.METHOD: The chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data.RESULT: Eight triterpenoid compounds were isolated and identified as ursolic acid (1), lupeol (2), alpha-amyrin (3), uvaol (4), 3beta-hydroxyurs-11-ene-13beta-olide (5), pomolic acid (6), lup-20 (29)-ene-3beta, 24-diol (7), 3beta, 23-dihydroxy-urs-12-en-28-oic acid (8).CONCLUSION: The eight compounds were obtained from this plant for the first time. [Studies on triterpenoid constituents in leaf of Ilex pernyi]. Xie GB, Zhou SX, Lei LD, Tu PF. No
18051899 OBJECTIVE: To study the chemical constituents of root of Actinidia macrosperma.METHOD: Chromatographic methods were used to isolate compounds from A. macrosperma and spectroscopic methods were used to identify the structures of the isolated compounds.RESULT: Eight compounds were obtained and identified as 12-oleanene-2alpha, 3alpha, 24-triol (1), isotachioside (2), asiatic acid (3), catechin (4), epicatechin (5), ursolic acid (6), beta-daucosterol (7), beta-sitosterol (8).CONCLUSION: All these compounds were isolated from this plant for the first time, compound 1, 2 were obtained from this genus for the first time. [Studies on chemical constituents from root of Actinidia macrosperma]. Ding LL, Wang SC, Wang ZT. No
18051900 OBJECTIVE: To study the chemical constituents from the roots and rhizome of Cynanchum atratum.METHOD: The chemical constituents were isolated and repeatedly purified by silica gel chromatography and the structures were elucidated by the NMR spectra and physicochemical properties.RESULT: Twelve compounds were obtained and nine of them were identified as 2, 4-dihyroxyacetophe none (1), 2, 6-dihyroxyacetophenone (2), 4-hydroxybenzenemethanol (3), benzoic acid (4), beta-amyrin acetate (5), palmitic acid (6), beta-sitosterol (7), beta-daucosterol (8), glaucogenin C-3-O-alpha-D-oleandropyranosyl-(1 --> 4)-beta-D-digitoxopyranosyl-(1 -->4)-alpha-D-oleandropyranoside (9).CONCLUSION: Seven compounds were obtained from C. atratum for the first time. [Studies on chemical constituents from root of Cynanchum atratum]. Yuan Y, Zhang WD, Zhang C, Liu RH, Su J, Jin HZ. No
18052029 Three new Securinega alkaloids, secu'amamines B-D ( 1- 3), were isolated from the wood of the Japanese medicinal plant Securinega suffruticosa var. amamiensis, together with five known analogues ( 4, 6- 9). The structures 1- 3 were elucidated by spectroscopic methods including 2D NMR, and all eight compounds were evaluated for cytotoxicity against two cancer cell lines. Securinega alkaloids from the wood of Securinega suffruticosa var. amamiensis. Ohsaki A1, Kobayashi Y, Yoneda K, Kishida A, Ishiyama H. No
18066132 Rosa nutkana Presl. (Rosaceae) is distributed abundantly throughout central and southern areas of British Columbia, Canada. Aboriginal people in the Pacific Northwest have traditionally used R. nutkana as a food, medicine, and source of cultural material. The methanolic extract of the fruits of R. nutkana was previously found to have inhibitory activity against methicillin-resistant Staphylococcus aureus (MRSA). In our study, bioactivity-guided fractionation of the methanol extract from R. nutkana led to the isolation of the following 10 compounds: (i) tormentic acid, (ii) euscaphic acid, (iii) ursolic acid, (iv) maslinic acid, (v) quercetin, (vi) catechin gallate, (vii) quercetin-3-O-glucoside, (viii) 1,2,3,4,6-penta-O-galloyl-beta-D-glucoside, (ix) L-ascorbic acid (vitamin C), and (x) 1,6-digalloyl-beta-D-glucoside. Structures were elucidated by ultraviolet, infrared, mass spectrometry, and nuclear magnetic resonance data, as well as by comparison with those of the literature. The compounds quercetin, catechin gallate, quercetin-3-O-glucoside, 1,2,3,4,6-penta-O-galloyl-beta-D-glucoside, and 1,6-digalloyl-beta-D-glucoside exhibited weak antibacterial activity against MRSA. Our research demonstrates the value of traditional knowledge held by Aboriginal people in the Pacific Northwest with respect to uses of R. nutkana. Some described uses in the ethnobotanical literature correspond to activities observed under laboratory conditions. Further work on British Columbia Rosa spp. may contribute to identifying other potential therapeutic uses Bioactivity-guided isolation of the active compounds from Rosa nutkana and quantitative analysis of ascorbic acid by HPLC. Jovel EM, Zhou XL, Ming DS, Wahbe TR, Towers GH. No
18069237 The aqueous methanolic extract of the aerial parts of Fagonia arabica L. (family Zygophyllaceae) was successively fractionated using certain organic solvents. From the ethyl acetate fraction, two flavonoid glycosides were isolated and identified as kaempferol-7-O-rhamnoside and acacetin-7-O-rhamnoside. Four triterpenoidal glycosides were isolated from the butanolic layer. Their structures were elucidated on the basis of the spectral and chemical data as 3-O-beta-D-glucopyranosyl-(1-->3)-alpha-L-arabinopyranoside oleanolic acid (1), 3-O-alpha-L-arabinopyranosyl quinovic acid 28-O-beta-D-glucopyranoside (2), 3-O-[beta-D-glucopyranosyl-(1-->2)]-beta-D-glucopyranosyl-(1-->3)-alpha-L-arabinosyl oleanolic acid (3) and 3-O-beta-D-glucopyranosyl-(1-->3)-alpha-L-arabino-pyranosyl quinovic acid 28-O-beta-D-glucopyranoside (4). The two monodesmosidic saponins 1 and 3 were found to possess strong molluscicidal activity against Biomphalaria alexandrina snails, the intermediate host of Schistosoma mansoni in Egypt (LC90 = 13.33 and 16.44 microM), whereas the other two bidesmosidic saponins 2 and 4 as well as the two flavonoid glycosides were inactive up to 50 microM. Phytochemical and molluscicidal investigations of Fagonia arabica. El-Wakil EA. No
18074843 OBJECTIVE: To study the chemical constituents of Gueldenstaeditia stenophylla.METHODS: The constituents were isolated by alcohol extraction, column chromatography on silica gel. Their structures were elucidated by chemical and spectroscopic methods.RESULTS: Six compounds were obtained, and five of them were identified as n-hexadecanioc acid (I), beta-sitosterol (II), daucosterol (III), apigenin (IV), D-fructose (VI). Compound V was being determined.CONCLUSION: Five compounds are isolated from Gueldenstaedtia stenophylla and compounds I, III are extracted from Gueldenstaedtia Fisch for the first time. [Studies on the chemical constituents of Gueldenstaedtia stenophylla]. Wei YX, Chen L, Wang JX. No
18074845 OBJECTIVE: To study the chemical constituents of Heterosmilax yunianensis.METHODS: The compounds were isolated and repeatedly purified with chromatograph and the structures were elucidated by physico-chemical properties and spectral analysis.RESULTS: Eight compounds were obtained and elucidated as beta-sitosterol (I), glycerol monopalmitate (II), daucosterol (IIl), hexacosanoic acid (IV), 5-hydroxymethyl furaldehyde (V), hergapen (VI), ursolic acid (VII), liquiritigenin (VIII).CONCLUSION: They have been isolated from this plant for the first time, and IV - VIII are obtained from the plants of Heterosmilax for the first time. [Studies on chemical constituents of Heterosmilax yunnanensis]. Qin WJ, Wang GL, Lin RC. No
18074850 Abstarct:"OBJECTIVE: To study the pharmacological effects of volatile oil of Valeriana amurensis on central nervous system.METHODS: The pharmacological effects of volatile oil of Valeriana amurensis on the autonomic activities of mice, the sleeping synergistic action of mice with pentoharbital sodium at subthreshold and hypnotic dosages, the sleep phases of rats, the writhing response of mice caused by acetic acid and the convulsion of mice induced by thio-semicarbazide were investigated.RESULTS: The autonomic activities of mice were significantly inhibited by the volatile oil of Valeriana amurensis. The rate of falling sleep and the extension of sleeping time of mice were significantly increased by the synergic action of pentobarbital sodium with the volatile oil. The sleep phases of SWS2 and REMS of rats were obviously extended by the volatile oil of Valeriana amurensis. In addition, the frequency of writhing response of mice caused by acetic acid was reduced, and the convulsion of mice induced by thio-semicarbazide was antagonized with the administration of the volatile oil.CONCLUSION: The volatile oil of Valeriana amurensis has significantly sedative analgesic and anti-hyperspasmia effects." Pharmacological effects of volatile oil of Valeriana amurensis on CNS Wu JK, Huo JH, Du XW No
18163556 Acacia confusa is traditionally used as a medicinal plant in Taiwan. In this study, phytochemicals and antioxidant activities of extracts from flowers of A. confusa were investigated for the first time. In addition, a rapid screening method, online RP-HPLC-DPPH system, for individual antioxidants in complex matrices was developed. Accordingly, six antioxidants including gallic acid ( 1), myricetin 3-rhamnoside ( 2), quercetin 3-rhamnoside ( 3), kaempferol 3-rhamnoside ( 4), europetin 3-rhamnoside ( 5), and rhamnetin 3-rhamnoside ( 6) were detected using the developed screening method. Of these, compounds 2, 3, and 5 were found to be major bioactive phytochemicals, and their contents were determined as 11.3, 6.7, and 8.7 mg/g of crude extract, respectively. By comparison with quercetin, a well-known antioxidant, these compounds had the order of compound 2 > compound 5 > quercetin > compound 3 for DPPH radical-scavenging activity. Their IC 50 values were 3.0, 3.2, 4.5, and 7.4 microM, respectively. Moreover, the same order was observed for superoxide radical-scavenging activity, and their IC50 values were 2.6, 2.7, 4.3, and 5.3 microM, respectively. However, for lipid peroxidation, quercetin, an aglycon, showed the best inhibitory activity. The IC50 values of quercetin, compound 2, compound 5, and compound 3 were 46.7, 88.5, 90.7, and 124.6 microM, respectively. These results indicated that a rhamnoside at the C3 position of flavonoids had a negative effect on radical-scavenging activity and antilipid peroxidation. In contrast, the number of hydroxyl groups on the B-ring exhibited a positive relationship with their inhibitory activities. Online RP-HPLC-DPPH screening method for detection of radical-scavenging phytochemicals from flowers of Acacia confusa. Wu JH, Huang CY, Tung YT, Chang ST. No
18165193 Composition of the essential oil obtained from dried flowering aerial parts of Teucrium scordium L. (Labiatae) was analyzed by GC and GC/MS. Fifty-six components were identified in the essential oil of T. scordium. The major constituents of the oil were beta-caryophyllene (22.8%), (E)-beta-farnesene (10.4%), caryophyllene oxide (8.6%), 1,8-cineole (6.1%) and beta-eudesmol (5.1%). Essential oil composition of Teucrium scordium L. Morteza-Semnani K, Saeedi M, Akbarzadeh M. No
18171023 Three new erythrina alkaloids, (+)-10,11-dioxoerythratine (1), (+)-10,11-dioxoepierythratidine (2), and (+)-10,11-dioxoerythratidinone (3), and a new pterocarpan, 1-methoxyerythrabyssin II (4), were isolated from the bark of Erythrina subumbrans, together with seven known pterocarpans, erythrabyssin II, erybraedin A, erystagallin A, erycristagallin, erythrabissin-1, eryvarin A, and hydroxycristacarpone, three flavanones, 5-hydroxysophoranone, abyssinone V, and lespedezaflavanone B, three triterpenes, sophoradiol, soyasapogenol B, and lupeol, and one isoflavanone, vogelin C. Their structures were elucidated on the basis of spectroscopic data. Some isolates were tested for antiplasmodial, antimycobacterial, and cytotoxic activities. Erythrina alkaloids and a pterocarpan from the bark of Erythrina subumbrans. Rukachaisirikul T1, Innok P, Suksamrarn A. No
18236747 Seven chemical constituents were isolated from the CHCl3 fraction and n-BuOH fraction from EtOH ext. of Liriope platyphylla Wang et Tang. Their structures were elucidated as lupenone (I), lupeol (II), ursolic acid (III), beta-sitosterol (IV), diosgenin (V), LP-A (VI) and LP-B (VII) respectively. Compound I, II and V were isolated from this genus of Liriope for the first time. Compound III and IV were isolated from this plant for the first time. [Studies on chemical constituents of Liriope platyphylla]. Jiang T, Huang BK, Zhang QY, Han T, Zheng HC, Qin LP. No
18236750 OBJECTIVE: To study the chemical constituents of flavonoids from Apocynum venetum L.METHODS: The compounds from water extracts were isolated by HPD-100 MCI-gel, polyamide, silica and Sephadex LH-20. Their structrues were characterized by chemical methods and spectroscopic methods.RESULTS: The five compounds were identified to be trifolin (I), hyperroside (III), hyperin (V), isoquercetin-6'-o-acetate (VII) and isoquercetin (VIII).CONCLUSION: Compound I and compound VII are isolated from Apocynum venetum L. for the first time. [Chemical constituents of flavonoids from Apocynum venetum]. Cheng XL, Zhang SQ, Li QS. No
18274278 Two new xanthones, characterized as 4-(1,1-dimethylprop-2-enyl)-1,3,5,8-tetrahydroxyxanthone (1) and penangianaxanthone (2), with three known xanthones, cudratricusxanthone H (3), macluraxanthone C (4) and gerontoxanthone C (5), as well as friedelin and stigmasterol were isolated from the leaves of Garcinia penangiana. Their structures were elucidated by analysis of spectroscopic data and comparison of the NMR data with the literature ones. Significant cytotoxicity against DU-145, MCF-7 and NCI-H460 cancer cell lines was demonstrated by compounds 1-5, with IC50 values ranging from 3.5 to 72.8 microM. Cytotoxic xanthones from Garcinia penangiana Pierre. Jabit ML, Khalid R, Abas F, Shaari K, Hui LS, Stanslas J, Lajis NH. No
18288811 Four new Daphniphyllum alkaloids, daphnioldhanines H-K ( 1- 4), along with 34 known alkaloids, were isolated from Daphniphyllum oldhami. The known alkaloid dehydrodaphnigraciline ( 5) is now reported as a natural product. Their structures were elucidated by spectroscopic methods, especially 2D NMR techniques. The effects against platelet aggregation of compounds 1, 3, and 5 were evaluated, and 3 showed stronger activity against platelet aggregation induced by PAF. This is the first report of quinolizidine alkaloids from the genus Daphniphyllum. Alkaloids from Daphniphyllum oldhami. Mu SZ1, Wang JS, Yang XS, He HP, Li CS, Di YT, Wang Y, Zhang Y, Fang X, Huang LJ, Hao XJ. No
18300491 OBJECTIVE: To study the chemical constituents of Phlomis younghusbandii.METHODS: Compounds were isolated from the ethanolic extract by silica gel column chromatography, and their structures were identified by physical and chemical evidences and spectral methods.RESULTS: Eight compounds were isolated and identified respectively as 8-acetylshanzhiside methyl ester (1), shanzhiside methyl ester (2), phlomiol (3), 2-butoxy-2-(hydroxymthyl) tetrahydro-2H-3,4,5-pyrantriol (4), sesamoside (5), pulchelloside-I (6), luteolin-7-O-beta-D-glucopyranoside (7) and daucosterol (8).CONCLUSION: All the compounds were isolated from the plant for the first time. [Studies on the chemical constituents of Phlomis younghusbandii]. Gao YL, Lin RC, Wang GL, Zhao HR, Gao Y, Ciren B. No
18300497 Six compounds were isolated and purified from Orobanche coerulescens by extraction and different kinds of column chromatography. The structures were determined on the basis of spectral analysis. The structures were elucidated as D-mannitol(I), beta-sitosterol(II), succinic acid(III), caffeic acid(IV), protocatechuic aldehyde(V) and daucosterol(VI). All compounds are obtained from this plant for the first time. [Study on chemical constituents of Orobanche coerulescens]. Zhao J, Yan M, Huang Y, He WY, Zhao Y. No
18309668 OBJECTIVE: To study the chemical constituents from the whole plants of Polygala telephioides.METHOD: Compounds were isolated by repeated silica gel and Sephadex LH -20 column chromatography, and their structures were determined by spectral analysis and physicochemical properties.RESULT: Six xanthones were isolated from P. telephioides, and their structures were identified as 1, 3, 7-trihydroxyxanthone (1), 1, 7-dihydroxy-3-methoxyxanthone (2), 1, 3-dihydroxyxanthone (3), 1, 7-dihydroxyxanthone (4), 1-methoxy-2, 3-methylenedioxyxanthone (5) and 1, 7-dimethoxyxanthone (6).CONCLUSION: All the compounds were obtained from this plant for the first time. [Studies on xanthones from herbs of Polygala telephioides]. Chang HT, Niu F, Wen J, Jiang Y, Tu PF. No
18313861 A new xanthone, yahyaxanthone (1), was isolated from Garcinia rigida leaves. Cytotoxicity evaluation showed that 1 was inhibitory to L1210 cell, with an IC50 value 4.08 microg/ml. A new cytotoxic xanthone from Garcinia rigida. Elya B, He HP, Kosela S, Hanafi M, Hao XJ. No
18323206 OBJECTIVE: To study the chemical constituents of Taxillus sutchuenenisis (Lecomte) Danser.METHODS: Chromatography and spectrum analysis were employed to isolated and elucidate the chemical constituents in the plant.RESULTS: 9 compounds were isolated and identified as quercetin (I), quervetin 3-O-beta-D-galactoside (II), isoquercitrin (III), quercitrin (IV), rutin (V), gallic acid (VI), ferulic acid (VII), beta-sitosterol (VIII), daucosterol (IX), respectively.CONCLUSION: Compounds III-IX are isolated from this plant for the first time. The work provide evidence for the exploitation and utilization of this plant resouce. [Studies on chemical constituents of Taxillus sutchuenenisis]. Chen JT, Feng F. No
18336006 Two new xanthone glycosides, corymbiferin 3-O-beta-D-glucopyranoside (1) and swertiabisxanthone-I 8'-O-beta- d-glucopyranoside (2), were isolated from Gentianella amarella ssp. acuta, along with eight known xanthones: triptexanthoside C, veratriloside, corymbiferin 1-O-glucoside, swertianolin, norswertianolin, swertiabisxanthone-I, bellidin, and bellidifolin, four of them identified for the first time in G. amarella ssp. acuta. The isolation was conducted mainly by centrifugal partition chromatography, and the structures of the isolated compounds were established on the basis of spectrometric data including 2D NMR and mass spectrometry. Xanthones were weakly active against acetylcholinesterase (AChE), except triptexanthoside C, which inhibited AChE with an IC(50) of 13.8 +/- 1.6 microM. Some compounds were active against monoamine oxidases (MAO): bellidin and bellidifolin showed interesting inhibitory activity of MAO A, while swertianolin, the 8-O-glucopyranoside form of bellidifolin, gave 93.6% inhibition of MAO B activity at 10(-5) M. Xanthones from Gentianella amarella ssp. acuta with acetylcholinesterase and monoamine oxidase inhibitory activities. Urbain A, Marston A, Grilo LS, Bravo J, Purev O, Purevsuren B, Batsuren D, Reist M, Carrupt PA, Hostettmann K. No
18336848 From a 1-BuOH-soluble fraction of the MeOH extract of leaves of Euodia meliaefolia, collected in Okinawa, seven megastigmane glucosides, named euodionosides A-G, were isolated together with three known megastgmane glucosides, and two aliphatic and three phenolic compounds. Their structures were elucidated through a combination of spectroscopic analyses and application of the modified Mosher's method. Euodionosides A-G: megastigmane glucosides from leaves of Euodia meliaefolia. Yamamoto M1, Akita T, Koyama Y, Sueyoshi E, Matsunami K, Otsuka H, Shinzato T, Takashima A, Aramoto M, Takeda Y. No
18341282 Sweet potato ( Ipomoea batatas) has been used as food and herb in many countries. In this research on the active constituents of sweet potato, nine compounds were isolated and identified, including seven new resin glycosides, batatosides A-G (1- 7), along with two known compounds, batatinoside I ( 8) and simonin IV ( 9). The structures of 1- 9 have been established by a combination of spectroscopic and chemical methods. The major characteristics of the new compounds are the presence of three different substituents. The absolute configuration of aglycones was established as S by Mosher's method. Batatoside E ( 5) showed weak cytotoxic activity against Hep-2 cells. Pentasaccharide glycosides from the tubers of sweet potato (Ipomoea batatas). Yin Y, Li Y, Kong L. No
18357734 To study the chemical constituents of Sparganium stoleniferum Buch. -Ham, various chromatographic techniques were used to separate and purify the chemical constituents. Their physicochemical properties and spectral data were used to elucidate the structures. Five compounds have been isolated by using silica gel column chromatography. They are beta-sitosterol plamitate (I), SanLeng diphenyllactone (II), SanLeng diphenylacetypene (III), 6,7,10-trihydroxy-8-octadecenoic acid (IV) and daucosterol plamitate (V). Compound II, III are two new compounds. [Chemical constituents of Sparganium stoleniferum]. Dong X, Wang GR, Yao QQ. No
18386487 Chromatographic separation of an ethyl acetate extract from Embelia schimperi led to the isolation of a new compound identified as 2,5-dihydroxy-3-methyl-1,4-benzoquinone (1) on the basis of spectroscopic and physical data. The plant's crude extract and pure compound 1 were assayed for in vitro antimicrobial activity against clinical strains of Salmonella spp., Proteus spp., Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Cryptococcus neoformans, Shigella dysentriae and Staphylococcus aureus. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antimicrobial activity. Standard antibiotics namely: augmentin, cotrimoxazole, gentamycin, tetracycline and lyncomycin were used as controls. The crude extract was inactive while the pure compound 1 showed significant activities against Salmonella spp., Proteus spp., Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Cryptococcus neoformans, Shigella dysentriae and Staphylococcus aureus with zones of inhibition ranging from 10-20 mm. The most sensitive microorganism was P aeruginosa while C. neoformans was insensitive to both the crude extract and compound 1. Antimicrobial activity of 2,5-dihydroxy-3-methyl-1,4-benzoquinone from Embelia schimperi. Awino OS, Kiprono PC, Keronei KP, Kaberia F, Obala AA. No
18387176 Abstarct:"BACKGROUND:Vector control is facing a threat due to the emergence of resistance to synthetic insecticides. Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. Although several plants have been reported for mosquitocidal activity, only a few botanicals have moved from the laboratory to field use, because they are poorly characterized, in most cases active principals are not determined and most of the works are restricted to preliminary screening. Solanum villosum is a common weed distributed in many parts of India with medicinal properties, but the larvicidal activity of this plant has not been reported so far.METHODS:Aqueous and polar/non-polar solvent extract of fresh, mature, green berries of S. villosum was tested against Stegomyia aegypti, a common vector of dengue fever. A phytochemical analysis of chloroform:methanol extract was performed to search for the active toxic ingredient. The lethal concentration was determined (log probit analysis) and compared with Malathion. The chemical nature of the active substance was also evaluated following ultraviolet-visual (UV-Vis) and infrared (IR) analysis.RESULTS:In a 72 hour bioassay experiment with the aqueous extract, the highest mortality was recorded in 0.5% extract. When the mortality of different solvent extracts was compared, the maximum (p < 0.05) mortality was recorded at a concentration of 50 ppm of chloroform:methanol extract (1:1, v/v). The larvicidal activity was lower when compared with the chemical insecticide, Malathion (p < 0.05). Results of regression analysis revealed that the mortality rate (Y) was positively correlated with the period of exposure (X) and the log probit analysis (95% confidence level) recorded lowest value (5.97 ppm) at 72 hours of exposure. Phytochemical analysis of the chlororm:methanol extract reported the presence of many bioactive phytochemicals. Two toxic compounds were detected having Rf = 0.82 (70% and 73.33% mortality in 24 and 48 hours, respectively) and Rf = 0.95 (40% and 50% mortality in 24 and 48 hours, respectively). IR analysis provided preliminary information about the steroidal nature of the active ingredient.CONCLUSION:S. villosum offers promise as potential bio control agent against S. aegypti particularly in its markedly larvicidal effect. The extract or isolated bioactive phytochemical could be used in stagnant water bodies for the control of mosquitoes acting as vector for many communicable diseases." Mosquito larvicidal activities of Solanum villosum berry extract against the dengue vector Stegomyia aegypti. Chowdhury N, Ghosh A, Chandra G. PMC2364612
18396905 Four new hasubanane-type alkaloids, periglaucines A-D (1-4), and three known alkaloids, norruffscine (5), (-)-8-oxotetrahydropalmatine (6), and (-)-8-oxocanadine (7), were isolated from the aerial parts of Pericampylus glaucus. Their structures were elucidated on the basis of extensive NMR and EIMS data, and that of periglaucine A (1) was confirmed by single-crystal X-ray diffraction. Alkaloids 1-4 inhibited hepatitis B virus (HBV) surface antigen (HBsAg) secretion in Hep G2.2.15 cells. (-)-8-Oxotetrahydropalmatine (6) possessed a high selectivity index (SI = 22.4) for HBsAg secretion of the Hep G2.2.15 cell line with an IC(50) value of 0.14 mM. Norruffscine (5) and (-)-8-oxotetrahydropalmatine (6) exhibited inhibitory activity against human immunodeficiency virus (HIV-1) with EC(50) values of 10.9 and 14.1 microM in C8166 cells (SI = 45.7 and 18.8), respectively. Periglaucines A-D, anti-HBV and -HIV-1 alkaloids from Pericampylus glaucus. Yan MH, Cheng P, Jiang ZY, Ma YB, Zhang XM, Zhang FX, Yang LM, Zheng YT, Chen JJ. No
18404325 From the fruits of Phaleria macrocarpa, icariside C(3) (1), phalerin (2), and mangiferin (3) were isolated and their structures were identified on the basis of spectroscopic data. Icariside C(3) (1) showed a slow vasorelaxant activity against noradrenaline-induced contraction of isolated rat aorta. The structure of phalerin (2) was revised as 2,4',6-trihydroxy-4-methoxybenzophenone-2-O-beta-D-glucoside. Studies on the constituents from the fruits of Phaleria macrocarpa. Oshimi S, Zaima K, Matsuno Y, Hirasawa Y, Iizuka T, Studiawan H, Indrayanto G, Zaini NC, Morita H. No
18415852 Three new caffeoyl galactoic acid derivatives, davidiosides A-C (1-3) together with four known caffeoyl derivatives such as: caffeic acid (4), methyl caffeate (5), chlorogenic acid (6), and methyl chlorogenate (7) were isolated from the leaves of Davidia involucrate. The new structures were determined by spectroscopic data and chemical evidence. New caffeoyl derivatives from the leaves of Davidia involucrata. Ouyang MA, Zhou JN, Wang SB. No
18415854 Two new phenolic water-soluble constituents, involcranoside A (1) and involcranoside B (2) have been isolated along with five known phenolic compounds: 3,4-dimethoxyphenyl-O-beta-D-gluco-pyranoside (3), picein (4), and 1,4-dihydroxy-3-methoxy-phenyl-4-O-beta-D-glucopyranoside (5), leonuriside A (6) and 4-hydroxy-3-methoxybenzoic acid (7) from the branch bark of Davidia involucrata. Identification of their structures was achieved by 1D and 2D NMR experiments, including (1)H-(1)H COSY, NOESY, HMQC and HMBC methods and FAB mass spectral data. Two new phenolic water-soluble constituents from branch bark of Davidia involucrata. Wu ZJ, Ouyang MA, Wang SB. No
18470799 Six new iridoidal glucosides, 2'-O-(3''-hydroxybenzoyl)-8-epikingiside (1), 2'-O-(3''-hydroxybenzoyl)-kingiside (2), 6'-O-p-coumaroyl-8-epikingiside (3), loganic acid 11-O-beta-glucopyranosyl ester (4), 6'-O-beta-glucopyranosyl secologanoside (5), and 6'-O-beta-glucopyranosyl secologanol (6), together with seven known iridoidal glucosides, loganic acid (7), 6'-O-beta-d-glucopyranosyl loganic acid (8), 8-epikingiside (9), kingiside (10), secologanoside (11), secoxyloganin (12), and alpigenoside (13), were isolated from the whole plant of Gentiana rhodantha (Gentianaceae). Their structures were elucidated by detailed spectroscopic analysis and chemical methods. Iridoidal glucosides from Gentiana rhodantha. Xu M, Wang D, Zhang YJ, Yang CR. No
18470814 A new secoiridoid, secamonoide A (1), and a new xanthone glycoside, secamonoide B (2), together with nine known compounds, were isolated from the aerial parts of Tylophora secamonoides Tsiang. Their structures were elucidated on the basis of spectroscopic methods. An antimicrobial bioassay showed that secamonoides A and B exhibited weak activities (MIC values greater than 100 mg/ml) against some hospital bacteria in vitro. Secoiridoids and xanthones from Tylophora secamonoides Tsiang. Yao S, Tang CP, Ye Y. No
18471018 The rare indazole-type alkaloid nigellidine (2) is accompanied by its 4-O-sulfite (4) in the seeds of Nigella sativa. Compound 4 may represent the true natural product leading to nigellidine (2) via hydrolysis of the sulfate functionality during the isolation process. The structure of nigellidine-4-O-sulfite (4) is confirmed by NMR, MS, and X-ray crystallographic data. This is the first report of the natural occurrence of sulfated indazole-type alkaloids. Nigellidine-4-O-sulfite, the first sulfated indazole-type alkaloid from the seeds of Nigella sativa. Ali Z1, Ferreira D, Carvalho P, Avery MA, Khan IA. No
18524603 Five new alkaloids, alstilobanines A (1)-E (5) were isolated from Alstonia angustiloba (Apocynaceae) and their structures were determined by MS and 2D NMR spectral analysis. Alstilobanines A-E showed a moderate vasorelaxant activity against phenylephrine-induced contraction of isolated rat aorta. Alstilobanines A-E, new indole alkaloids from Alstonia angustiloba. Koyama K1, Hirasawa Y, Zaima K, Hoe TC, Chan KL, Morita H. No
18533497 OBJECTIVE: To study the chemical constituents of Excoecaria agallocha L.METHOD: The constituents were isolated and purified by repeated column chromatography and their structures were elucidated by spectroscopic analysis.RESULT: Six triterpenoids including taraxerone (1), beta-amyrin acetate (2), 3beta-[(2E,4E)-6-oxo-decadienoyloxy]-olean-12-ene (3), taraxerol (4), acetylaleuritolic acid (5), and cycloart-22-ene-3beta, 25-diol (6), and three steroids including beta-sitostenone (7), (24R)-24-ethylcholesta-4,22-dien-3-one (8), and beta-sitosterol (9) were isolated and identified from the stems and twigs of the mangrove plant E. agallocha.CONCLUSION: Compounds 5-8 were isolated from E. agallocha for the first time. [Triterpenoids and steroids from Excoecaria agallocha]. Tian MQ, Bao GM, Ji NY, Li XM, Wang BG. No
18534639 As part of an ongoing search for antioxidants from medicinal plants, 20 constituents were isolated from the Nymphaea caerulea flowers, including two 2S,3S,4S-trihydroxypentanoic acid (1), and myricetin 3-O-(3''-O-acetyl)-alpha-L-rhamnoside (2), along with the known myricetin 3-O-alpha-L-rhamnoside (3), myricetin 3-O-beta-D-glucoside (4), quercetin 3-O-(3''-O-acetyl)-alpha-L-rhamnoside (5), quercetin 3-O-alpha-L-rhamnoside (6), quercetin 3-O-beta-D-glucoside (7), kaempferol 3-O-(3''-O-acetyl)-alpha-L-rhamnoside (8), kaempferol 3-O-beta-D-glucoside (9), naringenin (10), (S)-naringenin 5-O-beta-D-glucoside (11), isosalipurposide (12), beta-sitosterol (13), beta-sitosterol palmitate (14), 24-methylenecholesterol palmitate (15), 4alpha-methyl-5alpha-ergosta-7,24(28)-diene-3beta,4beta-diol (16), ethyl gallate (17), gallic acid (18), p-coumaric acid (19), and 4-methoxybenzoic acid (20). The structures were determined by spectroscopic means. Compounds were tested for antioxidant activity and nine compounds 2-7, 11, 12 and 18 were considered active with IC(50) of 1.16, 4.1, 0.75, 1.7, 1.0, 0.34, 11.0, 1.7 and 0.95 microg/ml, respectively, while 1 was marginally active (IC(50)>31.25 microg/ml). The most promising activity was found in the EtOAc fraction (IC(50) 0.2 microg/ml). This can be attributed to the synergistic effect of the compounds present in it. Antioxidant constituents of Nymphaea caerulea flowers. Agnihotri VK, Elsohly HN, Khan SI, Smillie TJ, Khan IA, Walker LA. No
18536375 OBJECTIVE: To study the chemical constituents in Huangjuhua (flowers of Chrysanthemum morifolium).METHOD: The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by NMR and MS evidences.RESULT: Eleven compounds were obtained and identified as luteolin (1), quercetin (2), acacetin 7-O-beta-D-(3"-acetyl) -glucopyranoside (3), luteolin 7-O-beta-D-(6"-acetyl)-glucopyranoside (4), hesperetin 7-O-beta-D-glucopyranoside (5), acacetin 7-O-beta-D-glucopyranoside (6), diosmetin 7-O-beta-D-glucopyranoside (7), apigenin 7-O-beta-D-glucopyranoside (8), hesperidin (9), linarin (10), and luteolin 7-O-beta-D-glucopyranoside (11).CONCLUSION: Compounds 4, 5 were isolated from the plant for the first time. [Studies on chemical constituents in Huangjuhua (flowers of Chrysanthemum morifolium)]. Wang YJ, Yang XW, Guo QS. No
18553922 Four new Daphniphyllum alkaloids, daphlongamines A-D (1-4), were isolated from the fruits of Daphniphyllum longeracemosum. Their structures were characterized by spectroscopic methods, especially 2D NMR techniques. A single-crystal X-ray diffraction analysis was used to confirm the stereochemistry of 3. Remarkably, this is the first report of aconitine- and veatchine-type diterpenoid alkaloids from the genus Daphniphyllum. Daphniphyllum and diterpenoid alkaloids from Daphniphyllum longeracemosum. Li CS1, Di YT, Mu SZ, He HP, Zhang Q, Fang X, Zhang Y, Li SL, Lu Y, Gong YQ, Hao XJ. No
18553925 A new prenylated xanthone, pinetoxanthone (1), and two new pyranochromanones, pinetoric acid I (2) and pinetoric acid II (3), together with 10 known compounds, namely, the triterpenes friedelin and canophyllol, the xanthone macluraxanthone, the pyranochromanone derivatives calophyllic acid, isocalophyllic acid, calolongic acid, apetalic acid, and isoapetalic acid, and the flavonoids amentoflavone and apigenin, were isolated from the stem bark and leaves of Calophyllum pinetorum, an endemic species in Cuba. The structures of 1-3 were elucidated by spectroscopic methods including 1D and 2D NMR experiments as well as HRESIMS analysis. Constituents of the Cuban endemic species Calophyllum pinetorum. Alarcón AB, Cuesta-Rubio O, Pérez JC, Piccinelli AL, Rastrelli L. No
18560334 Two new dibenzocyclooctadiene lignans, neglschisandrins C-D (1-2), were isolated from the stems of Schisandra neglecta. Their structures and stereochemistries were elucidated by spectroscopic methods, including 1D- and 2D-NMR and HR-ESI-MS techniques. Neglschisandrins C-D: two new dibenzocyclooctadiene lignans from Schisandra neglecta. Chen M, Liao Z, Xu X, Wen Y, Sun M, Zhang H, Ma W. No
18578539 Six new nortriterpenoids, propindilatones E-J (1-6), and two known (7, 8) schiartane-type nortriterpenoids were isolated from the stems of Schisandra propinqua var. propinqua. Their structures were elucidated by extensive spectroscopic analyses, and the structure of compound 4 was confirmed through single-crystal X-ray diffraction. The absolute configuration of compounds 1-3 was established using CD methods. Compounds 4-6 were noncytotoxic against K562, A549, and HT-29 human cancer cells. Propindilactones E-J, Schiartane Nortriterpenoids from Schisandra propinqua var. propinqua. Lei C, Huang SX, Chen JJ, Yang LB, Xiao WL, Chang Y, Lu Y, Huang H, Pu JX, Sun HD. No
18589747 OBJECTIVE: To study the chemical constituents of Primula sikkmensis Hook.METHODS: The chemical constituents of the plant were isolated and purified by column chromatography and their structures were elucidated by spectroscopic analysis.RESULTS: Seven compounds were isolated and identified as kaempferol, quercetin, Prunetin, hexacosanol, Apigenin, beta-sitosterol, quercetin-3-O-beta-D-glucoside, respectively.CONCLUSION: Compounds I--VII are obtained for the first time from this plant. [Studies on chemical constituents of Primula sikkmensis]. Peng T, Tu YQ, Deng Y, Zhang X. No
18589748 OBJECTIVE: To study chemical constituents of Changium smyrnioides Wolff.METHODS: The chemical components were isolated and purified by silica gel column and recrystallization. The chemical structures were elucidated on the basis of physico-chemical properties and spectral data.RESULTS: Ten compounds were isolated and identified as lignoceric acid (1), beta-sitosterol (2), stigmasterol (3), 5-hydroxy-8-methoxypsoralen (4), glycerylmonopalmitate (5), L-pyroglutamic acid (6), succinic acid (7), vanillic acid-4-O-beta-D-glucopyranoside (8 ), vanillic acid (9), daucosterol (10).CONCLUSION: Compounds 1, 4, 5, 6, 8 and 9 are obtained from the plant for the first time. [Chemical constituents of Changium smyrnioides]. Ren DC, Qian SH, Yang NY, Xie N, Duan JA. No
18589779 OBJECTIVE: To study the chemical constituents of Securidaca inappendiculata.METHOD: Column chromatographic techniques were applied to isolate constituents. A combination of MS and NMR spectroscopy was used to identify structures of constituents.RESULT: Seven compounds were isolated from the CHCl3 fraction and their structures were elucidated as 3, 4-dihydroxybezoic acid (1), 3-hydroxy-4-methoxylbenzoic acid (2), coniferaldehyde (3), 7-hydroxy-1, 2, 3, 8-tetrame thoxyxanthone (4), 1, 3, 8-trihydroxy-4-methoxyxanthone (5), 1, 3, 8-trihydroxy-2-methoxyxanth-one (6), 1, 3, 6-trihydroxy-2,7-dimethoxyxanthone (7).CONCLUSION: Compounds 1-6 were isolated for the first time from the genus. The antioxidant activities of compounds 3-6 were assayed by the method of DPPH and compound 3 showed the IC50 value of 4.14 microg x mL(-1). [Study on chemical constituents in rhizome of Securidaca inappendiculata]. Shi L, Kang WY. No
18590312 Ten new highly oxygenated triterpenoids, kadcoccilactones A-J (1-10), and two known triterpenoids, kadsuphilactone A and micrandilactone B, were isolated from the stems of the evergreen climbing plant Kadsura coccinea. The structures of the new compounds were elucidated by spectroscopic evidence, with that of 1 confirmed by single-crystal X-ray diffraction analysis. This is the first report on nortriterpenoids (2, 4-6, 8-10) from the genus Kadsura. Kadcoccilactones A-J, triterpenoids from Kadsura coccinea. Gao XM, Pu JX, Huang SX, Lu Y, Lou LG, Li RT, Xiao WL, Chang Y, Sun HD. No
18619237 OBJECTIVE: To investigate the chemical constituents from Anoectochilus roxburghii.METHODS: The compounds were isolated and purified by repeated column chromatographies with silica gel, Macroporous resin and Sephadex LH-20, and their structures were identified by their physical and spectral datas.RESULTS: Ten compounds were isolated and elucidated as: beta-D-glucopyranosyl-(3R)-hydroxybutanolide (I), stearic acid (II), palmitic acid (III), beta-sitosterol (IV) and succinic acid (V), p-hydroxy benzaldehyde (VI), daucosterol (VII), and methyl 4-beta-D-glucopyranosyl-hutanoate (VIII); as well as p-hydroxy cinnamic acid (IX) and o-hydroxy phenol (X) were identified.CONCLUSION: Compound I ,VIII, X are firstly isolated from this species. [Studies on chemical constituents from Anoectochilus roxburghii]. Cai JY, Gong LM, Zhang YH, Ruan HL, Pi HF, Wu JZ. No
18661821 OBJECTIVE: To study the chemical constituents of the roots of Angelica pubescens.METHODS: Fresh roots of Angelica pubescens were extracted with EtOH,the chemical constituents in the extract were isolated by column chromatography as macroporous absorption resin,and ODS etc. Their structures were confirmed by spectral analysis (NMR, MS).RESULTS: Seven compounds were isolated and their structures were identified as nodakenetin (I), umbelliferone (II), columbianetin (III), beta-D-glucosyl-columbianetin (IV), columbianin (V), tert. -O-beta-D- glucopyranosyl -(R)-by-akangelicin (VI) and sec. -O-beta-D- glucopyranosyl-(R) -byakangelicin (VII).CONCLUSION: Compound IV, VI and VII are isolated from Angelica pubescens for the first time. [Studies on chemical constituents of the roots of Angelica pubescens]. Ding XF, Feng X, Dong YF, Zhao XZ, Chen Y, Wang M. No
18679750 The antifungal activity of essential oil (EO) from the Brazilian epazote (Chenopodium ambrosioides L.) was evaluated by the poison food assay at concentrations of 0.3%, 0.1%, and 0.05% with eight postharvest deteriorating fungi (Aspergillus flavus, Aspergillus glaucus, Aspergillus niger, Aspergillus ochraceous, Colletotrichum gloesporioides, Colletotrichum musae, Fusarium oxysporum, and Fusarium semitectum). EO components were tentatively identified by Kováts retention indices (RIs) using gas chromatography and gas chromatography combined with mass spectrometry (GC-MS). Growth of all fungi was completely inhibited at 0.3% concentration, and by 90% to 100% at 0.1% concentration. The following 13 tentatively identified compounds (relative percent) accounted for 90.4% of the total volatile oil: alpha-terpinene (0.9), p-cymene (2.0), benzyl alcohol (0.3), p-cresol (0.3), p-mentha-1,3,8-triene (0.2), p-cimen-8-ol (0.6), alpha-terpineol (0.5), (Z)-ascaridole (61.4), piperitone (0.9), carvacrol (3.9), (E)-ascaridole (18.6), (E)-piperitol acetate (0.5), and (Z)-carvyl acetate (0.3). Autobiographic thin layer chromatography of the EO to separate the principal fungitoxic fraction yielded only one fraction that completely inhibited the growth of all test fungi at a concentration of 0.1%. This fraction was characterized by RIs and GC-MS presenting a composition (%) of p-cymene (25.4), (Z)-ascaridole (44.4), and (E)-ascaridole (30.2). The results suggest ascaridoles were the principal fungitoxic components of the EO. Composition and antifungal activity of the essential oil of the Brazilian Chenopodium ambrosioides L. Jardim CM, Jham GN, Dhingra OD, Freire MM. No
18691858 The compounds of an aqueous root extract of the African medicinal plant Pelargonium sidoides were analysed by LC-MS spectroscopy and the antiviral effect of this extract against herpes simplex virus was examined in cell culture. Besides predominant coumarins, simple phenolic structures as well as flavonoid and catechin derivatives were identified as major constituents in the Pelargonium extract. The inhibitory activity of this extract against herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2) was tested in vitro on RC-37 cells using a plaque reduction assay and exhibited high antiviral activity against both herpesviruses in viral suspension tests. The 50% inhibitory concentration (IC(50)) of the aqueous Pelargonium sidoides extract for herpes simplex virus plaque formation was determined at 0.00006% and 0.000005% for HSV-1 and HSV-2, respectively. At maximum noncytotoxic concentrations of the extract, plaque formation was significantly reduced by more than 99.9% for HSV-1 and HSV-2 and a clear concentration-dependent antiviral activity against HSV could be demonstrated for this extract. In order to determine the mode of antiviral action, the extract was added at different times to the cells or viruses during the infection cycle. Both herpesviruses were significantly inhibited when pretreated with the plant extract or when the extract was added during the adsorption phase, whereas acyclovir demonstrated antiviral activity only intracellularly during replication of HSV. These results indicate that P. sidoides extract affected the virus before penetration into the host cell and reveals a different mode of action when compared to the classical drug acyclovir. Hence this extract is capable of exerting an antiviral effect on herpes simplex virus and might be suitable for topical therapeutic use as antiviral drug both in labial and genital herpes infection. Efficacy of an aqueous Pelargonium sidoides extract against herpesvirus. Schnitzler P, Schneider S, Stintzing FC, Carle R, Reichling J. No
18693101 Extracts and essential oils of medicinal plants are increasingly of interest as novel drugs of antimicrobial and antiviral agents, since herpes simplex virus (HSV) might develop resistance to commonly used antiviral agents. Melissa officinalis essential oil was phytochemically examined by GC-MS analysis, its main constituents were identified as monoterpenaldehydes citral a, citral b and citronellal. The antiviral effect of lemon balm oil, the essential oil of Melissa officinalis, on herpes simplex virus was examined. The inhibitory activity against herpes simplex virus type 1 (HSV-1)and herpes simplex virus type 2 (HSV-2) was tested in vitro on monkey kidney cells using a plaque reduction assay. The 50% inhibitory concentration (IC50) of balm oil for herpes simplex virus plaque formation was determined at high dilutions of 0.0004% and 0.00008% for HSV-1 and HSV-2, respectively. At noncytotoxic concentrations of the oil,plaque formation was significantly reduced by 98.8% for HSV-1 and 97.2% for HSV-2, higher concentrations of lemon balm oil abolished viral infectivity nearly completely. In order to determine the mode of antiviral action of this essential oil, time-on-addition assays were performed. Both herpesviruses were significantly inhibited by pretreatment with balm oil prior to infection of cells. These results indicate that Melissa oil affected the virus before adsorption, but not after penetration into the host cell, thus lemon balm oil is capable of exerting a direct antiviral effect on herpesviruses. Considering the lipophilic nature of lemon balm essential oil, which enables it to penetrate the skin, and a high selectivity index, Melissa officinalis oil might be suitable for topical treatment of herpetic infections. Melissa officinalis oil affects infectivity of enveloped herpesviruses. Schnitzler P, Schuhmacher A, Astani A, Reichling J. No
18717338 To investigate the chemical constituents of the leaves of Adinandra nitida, several column chromatography methods were used to isolate the chemical constituents of this plant. The structures were elucidated on the basis of spectral data. Six compounds were isolated and identified as 2alpha, 3alpha, 19alpha-trihydroxy-olean-12-en-28-oic acid-28-O-beta-D-glucopyranoside (1), arjunetin (2), sericoside (3), glucosyl tormentate (4), nigaichigoside F1 (5) and arjunglucoside I (6), separately. Compound 1 is a new compound, and compounds 2 -6 were isolated from A. nitida for the first time. [Triterpene saponins from Adinandra nitida]. Wang Y, Ye WC, Yin ZQ, Zhao SX. No
18720865 OBJECTIVE: To investigate the chemical constituents of the herbs of Taraxacum mongolicum.METHOD: The chemical constituents were isolated by various column chromatographic methods and their structures elucidated mainly by NMR and MS evidences.RESULT: Forty-four components were obtained and identified were as artemetin (1), quercetin (2), quercetin-3', 4', 7-trime-thyl ether (3), luteolin (4), luteolin-7-O-beta-D-glucopyranoside (5), luteolin-7-O-beta-D-galactopyranoside (6), genkwanin (7), isoetin (8), hesperetin (9), genkwanin-4'-O-beta-D-lutinoside (10), hesperidin (11), quercetin-7-O-[beta-D-glucopyranosyl (1-->6) -beta-D-glucopyranoside (12), quercetin-3, 7-O-beta-D-diglucopyranoside (13), isoetin-7-O-beta-D-glucopyranosyl- 2'-O-alpha-L-arabinopyranoside (14), isoetin-7-O-beta-D-glucopyranosyl-2'-O-alpha-D-glucopyranoside (15), isoetin-7- O-beta-D-glucopyranosyl-2'-O-beta-D-xyloypyranoside (16), caffeic acid (17), furulic acid (18), 3-O-caffeoylquinic acid (19), 3, 5-di-O-caffeoylquinic acid (20), 3, 4-di-O-caffeoylquinic acid (21), 4, 5-di-O-caffeoylquinic acid (22), 1-hydroxymethyl-5-hydroxy-phenyl-2-O-beta-D-glucopyranoside (23), p-hydroxybenzoic acid (24), p-coumaric acid (25), 3, 5-dihydroxylbenzoic acid (26), gallic acid (27), gallicin (28), syringic acid (29), 3, 4-dihydroxybenzoic acid (30), caffeic acid ethyl ester (31), esculetin (32), rufescidride (33), mongolicumin A [6, 9, 10-trihydroxy-benzoxanthene-1, 2-dicarboxylic acid] (34), mongolicumin B [1 l-hydroxy-2-oxo-guaia-1 (10), 3, 5-trien-8, 12-lactone] (35), isodonsesquitin A (36), taraxacin (37), sesquiterpene ketolactone (38), taraxasteryl acetate (39), phi-taraxasteryl acetate (40) and lupenol acetate (41), palmitic acid (42), beta-sitosterol (43), and stigmasterol (44).CONCLUSION: Four compounds (14, 15, 34 and 35) were new compounds, compounds 1, 3, 6-13, 20-22, 30 and 31 were isolated from this genus for the first time, while compounds 18, 23-29, 32 and 37-42 were obtained from this species for the first time. [Studies on chemical constituents from herbs of Taraxacum mongolicum]. Shi SY, Zhou CX, Xu Y, Tao QF, Bai H, Lu FS, Lin WY, Chen HY, Zheng W, Wang LW, Wu YH, Zeng S, Huang KX, Zhao Y, Li XK, Qu J. No
18725180 A CHCl3-soluble fraction of 70% EtOH extract of the flower of Kayea assamica from Myanmar exhibited 100% preferential cytotoxicity (PC(100)) against human pancreatic cancer PANC-1 cells under nutrient-deprived conditions at 1 microg/mL. Bioassay-guided fractionation and isolation afforded nine new coumarins, kayeassamins A (8), B (9), and C-I (1-7), together with nine known coumarins (10-18). The structures of these compounds were identified by extensive spectroscopic techniques as well as by comparison with published data. Absolute configuration at C-1' of 1 was established as S-configuration by the modified Mosher method. All the isolates were evaluated for their in vitro preferential cytotoxicity using novel anti-austerity strategy. Among them, the novel coumarins, kayeassamins A (8), B (9), D (2), E (3), and G (5) exhibited the most potent preferential cytotoxicity (PC(100) 1 microM) in a concentration- and time-dependent manner and induced apoptosis-like morphological changes of PANC-1 cells within 24 h of treatment. Based on the observed cytotoxicity, structure-activity relationships have been established. Novel anticancer agents, kayeassamins C-I from the flower of Kayea assamica of Myanmar. Win NN, Awale S, Esumi H, Tezuka Y, Kadota S. No
18761075 ETHNOPHARMACOLOGICAL RELEVANCE:

Ethnopharmacological survey indicated leaves of Byrsonima fagifolia Nied. (Malpighiaceae) against gastrointestinal disorders.
AIM OF THE STUDY:

The methanolic extract from the leaves of Byrsonima fagifolia (denominated BF) was evaluated for toxic, mutagenic, gastroprotective, antidiarrheal, antibacterial and immunomodulatory activities.
MATERIALS AND METHODS:

The preventive and healing action of BF against gastric ulcer was evaluated in experimental models in rodents. We evaluated immunomodulatory (by murine peritoneal macrophages), antidiarrheal (by induced diarrhea with castor oil and intestinal motility) and antibacterial action of BF against standard strain of Escherichia coli, Staphylococcus aureus and Helicobacter pylori. The safety of use of BF was also evaluated by mutagenic (Ames assay) and by analyses of toxicity parameters.
RESULTS:

Phytochemical BF profile indicated the presence of phenolic compounds with antioxidant and radical-scavenging properties. BF significantly inhibited gastric lesions induced by ethanol and HCl/ethanol and endogenous mucosal sulphydryl groups (SHs) participated efficaciously in BF gastroprotection. BF blocked development of inflammation process and also has antidiarrheal actions. This extract accelerated the healing of the gastric ulcerated mucosa by stimulating proliferative factors and by increasing production of gastric mucus with no toxic action. The substances responsible for the protective action are concentrated in the ethyl acetate fraction that demonstrated no mutagenic action in vitro.
CONCLUSIONS:

Byrsonima fagifolia presents gastroprotective, healing and antidiarrheal activities supporting previous claims that its traditional use by Brazilians can treat these gastrointestinal ailments.
Byrsonima fagifolia: an integrative study to validate the gastroprotective, healing, antidiarrheal, antimicrobial and mutagenic action. Lima ZP, dos Santos Rde C, Torres TU, Sannomiya M, Rodrigues CM, dos Santos LC, Pellizzon CH, Rocha LR, Vilegas W, Souza Brito AR, Cardoso CR, Varanda EA, de Moraes HP, Bauab TM, Carli C, Carlos IZ, Hiruma-Lima CA. No
18778099 Seven new indole alkaloids of the Aspidosperma type, jerantinines A-G (1-7), were isolated from a leaf extract of the Malayan Tabernaemontana corymbosa. The structures were established using NMR and MS analysis. Five of the alkaloids isolated and two derivatives (1-5, 8, 9) displayed pronounced in vitro cytotoxicity against human KB cells (IC50 < 1 microg/mL). Jerantinines A-G, cytotoxic Aspidosperma alkaloids from Tabernaemontana corymbosa. Lim KH1, Hiraku O, Komiyama K, Kam TS. No
18790035 Dendrophthoe falcata (L.f) Ettingsh (Loranthaceae) of the order Santalales, is used ethnomedicinally for treating ulcers, asthma, impotence, paralysis, skin diseases, and wounds. In this context, validations of the ethnotherapeutic claims of the plant in wound healing activity was studied, besides anti-microbial activity and antioxidant activity were performed to understand the mechanism of wound healing potency. The ethanolic extract of aerial parts of Dendrophthoe falcata ethanolic extract (DFEE) was investigated for the evaluation of its healing efficiency on excision and incision wound models in rats. The results showed that Dendrophthoe falcata extract has potent wound healing capacity as evident from the wound contraction and increased tensile strength. Hydroxyproline and hexosamine expressions were also well correlative with the healing pattern observed. Three of the fractions A-C (petroleum ether, chloroform and ethanol, respectively) obtained from the extract exhibited significant antimicrobial activity against the organisms: Staphylococcus aureus, Staphylococcus pyogenes, Staphylococcus epidermidis, Micrococcus luteus, Bacillus subtilis, Bacillus cereus, Klebsiella pneumoniae, Enterobacter aerogenes, Pseudomonas aeroginosa, Serratia marcescens, and five fungi Candida albicans, Candida tropicalis: dimorphic fungi, Aspergillus fumigatus, Aspergillus niger: systemic fungi, and some infectious bacteria Escherichia coli, and Salmonella typhi. The results also indicated that DFEE possesses potent antioxidant activity by inhibiting lipid peroxidation, reduced glutathione, superoxide dismutase levels and increased the catalase activity. Wound healing, anti-microbial and antioxidant potential of Dendrophthoe falcata (L.f) Ettingsh. Pattanayak SP, Sunita P. No
18811206 Twelve new alkaloids, caldaphnidines G-R (1-12), along with 24 known ones, were isolated from the twigs of Daphniphyllum calycinum. Their structures were elucidated by spectroscopic methods, especially two-dimensional NMR techniques. Alkaloids from the twigs of Daphniphyllum calycinum. Zhang CR1, Yang SP, Yue JM. No
18826143 OBJECTIVE: To study the chemical constituents of Syringa veutina Kom.METHODS: The constituents were isolated and repeatedly purified on silica and Sephadex LH-20 column chromatography. They were dentified and structurally elucidated by spectral analysis.RESULTS: Seven compounds were obtained. They were identified as Syningin(1), Liriodendrin(2), (+) -Medioresinol di-O-beta-D-glucopyranosied (3), Rutin(4), Quercetin 3-O-beta-D-glucopyranoside (5), Kaempferol-3-O-beta-D-glucopyranosied (6), D-mannitol (7).CONCLUSION: Compound 2 and 3 were isolated from this genus for the first time. Compound 4, 5, 6 and 7 were isolated from this plant for the first time. [Studies on chemical constituents of Syringa veutina]. Zhou LG, Feng XS, Huang KY, He L, Deng XM, Wang DC. No
18830630 The petroleum ether extract of Viola websteri Hemsl. (Violaceae) was investigated for its activity against chloroquine-sensitive strains of Plasmodium falciparum using the parasite lactate dehydrogenase assay method. The main antiplasmodial principles, 6-(8'Z-pentadecenyl)-salicylic acid (1) and 6-(8'Z, 11'Z, 14'Z-heptadecatrienyl)-salicylic acid (2), have been isolated from V. websteri for the first time. This is the first report on the antiplasmodial activity of the compounds from V. websteri. Bioassay-guided isolation of antiplasmodial anacardic acids derivatives from the whole plants of Viola websteri Hemsl. Lee SJ, Park WH, Moon HI. No
18837345 OBJECTIVE: To study the chemical constituents in the aerial part of Acalypha australis.METHOD: The aerial part of Acalypha australis were extracted with EtOH at room temperature. The compounds were separated by various kinds of chromatographic methods and their structures were identified on the basis of spectroscopic techniques.RESULT: Eleven compounds have been isolated and identified: emodin (1), beta-sitosterol (2), loliolide (3), 2,6-dimethoxy-1,4-benzoquinone (4), nicotinic acid (5), protocatechuic acid (6), daucosterol (7), gallic acid (8), rutin (9), succinic acid (10), brevifolin (11).CONCLUSION: Compounds 1, 3-5, 10 and 11 were obtained from A. australis for the first time. [Chemical constituents of aerial part of Acalypha australis]. Wang XL, Yu KB, Peng SL. No
18844422 Phytochemical investigation of leaves of Macaranga tanarius resulted in the isolation of seven new prenylated flavanones, macaflavanones A-G (1-7), along with two known compounds, nymphaeol C (9) and the diterpene kolavenol. The structures of the new compounds were elucidated by means of a combination of spectroscopic methods and chemical conversion. The absolute structure of tanariflavanone B (8), isolated from the title plant, was also resolved. The cytotoxic activities of isolated flavanones were assayed using two cell lines, with macaflavanone G (7) being the most active compound in each case. Macaflavanones A-G, prenylated flavanones from the leaves of Macaranga tanarius. Kawakami S, Harinantenaina L, Matsunami K, Otsuka H, Shinzato T, Takeda Y. No
18848979 Abstarct:"AIM OF THE STUDY:Nine plants from Reunion Island, selected using ethnopharmacology and chemotaxonomy, were investigated for their potential antimalarial value.MATERIALS AND METHODS:Thirty-eight extracts were prepared by maceration using CH(2)Cl(2) and MeOH, and were tested for in vitro activity against the 3D7 and W2 strain of Plasmodium falciparum. The most active extracts were then tested for in vitro cytotoxicity on human WI-38 fibroblasts to determine the selectivity index. Those extracts were also investigated in vivo against Plasmodium berghei infected mice.RESULTS:Most active of the extracts tested were the dichloromethane leaves extracts of Nuxia verticillata Lam. (Buddlejaceae), Psiadia arguta Voigt. (Asteraceae), Lantana camara L. (Verbenaceae), the methanol extracts from Aphloia theiformis (Vahl) Benn. (Aphloiaceae) bark, and Terminalia bentzoe L. (Combretaceae) leaves displaying in vitro IC(50) values ranging from 5.7 to 14.1mug/ml. Extracts from Psiadia, Aphloia at 200mg/(kgday) and Teminalia at 50mg/(kgday) also exhibited significant (p<0.0005) parasite inhibition in mice: 75.5%, 65.6% and 83.5%, respectively.CONCLUSION:Two plants showed interesting antimalarial activity with good selectivity: Aphloia theiformis and Terminalia bentzoe. Nuxia verticillata still needs to be tested in vivo, with a new batch of plant material." Screening of medicinal plants from Reunion Island for antimalarial and cytotoxic activity. Jonville MC, Kodja H, Humeau L, Fournel J, De Mol P, Cao M, Angenot L, Frédérich M. No
18951592 Nortriterpenoids, sphenadilactone C (1) and sphenasin A (2), together with four known lignans (3-6), were isolated from the leaves and stems of Schisandra sphenanthera. Their structures were elucidated by extensive analysis of 1D and 2D NMR spectroscopic data and compound 2 was further confirmed by single-crystal X-ray diffraction. Compound 1 features a partial enol moiety and an acetamide group in its structure. In addition, compounds 1, 3-6 showed weak anti-HIV-1 activity with EC(50) values in the range of 15.5-29.5 microg/mL. Nortriterpenoids and lignans from Schisandra sphenanthera. Xiao WL, Huang SX, Wang RR, Zhong JL, Gao XM, He F, Pu JX, Lu Y, Zheng YT, Zheng QT, Sun HD. No
18955262 Methanolic extracts of 41 plant species belonging to 27 families used in the traditional medicine in Nepal have been investigated for in vitro antiviral activity against Herpes simplex virus type 1 (HSV-1) and influenza virus A by dye uptake assay in the systems HSV-1/Vero cells and influenza virus A/MDCK cells. The extracts of Astilbe rivularis, Bergenia ciliata, Cassiope fastigiata and Thymus linearis showed potent anti-herpes viral activity. The extracts of Allium oreoprasum, Androsace strigilosa, Asparagus filicinus, Astilbe rivularis, Bergenia ciliata and Verbascum thapsus exhibited strong anti-influenza viral activity. Only the extracts of A. rivularis and B. ciliata demonstrated remarkable activity against both viruses. Antiviral activity of some plants used in Nepalese traditional medicine. Rajbhandari M, Mentel R, Jha PK, Chaudhary RP, Bhattarai S, Gewali MB, Karmacharya N, Hipper M, Lindequist U. No
18956776 Radix Sophorae Flavescentis is a traditional Chinese medicine, which is the roots of Sophora flavescens and is used to treat abscess, dysentery, eczema and skin itch. The main active compounds of the roots of Sophora flavescens are alkaloids and flavonoids. To provide reference compounds for HPLC-DAD qualitification analysis of flavanonoids and for study on the bioactivities of flavanonoids, six main flavanonoids were isolated from the roots of Sophora flavescens. They are 5-methoxy-7, 2', 4'-trihydroxy-8-[3,3-dimethylallyl] -flavanone (1), isoxanthohumol (2), 3/beta, 7, 4'-trihydroxy-5-methoxy-8-[3,3-dimethylallyl] -flavanone (3), nor-kurarinone [(2S) -sophoraflavanone G] (4), kurarinone (5) and kushenol I (6). Compound 1 is a new compound, and compound 3 is obtained from Sophora flavecens for the first time. The 13C NMR data of compounds 3 and 5 are reported for the first time. [Main flavonoids from Sophora flavescenes]. Li W, Liang H, Yin T, Wang B, Zhao YY. No
18970387 Four flavonoids (rutin, hyperoside, quercitrin and quercetin) in Houttuynia cordata Thunb. and Saururus chinensis (Lour.) Bail. were determined by capillary electrophoresis with wall-jet amperometric detection. The working electrode was a 500 microm diameter carbon disc electrode and the detection potential was +0.95 V (versus Ag/AgCl). Effects of several important factors, such as the running buffer and its corresponding pH and concentration, separation voltage, injection time were investigated to acquire the optimum conditions for separation of these four flavonoids. Baseline separation for the four flavonoids was obtained within 21 min in a 60 cm length capillary at a separation voltage of 15 kV with a 60 mmoL/L Na(2)B(4)O(7)-120 mmoL/L NaH(2)PO(4) buffer (pH 8.8) as running buffer. The relationship between peak currents and analyte concentrations was linear over about two orders of magnitude with detection limits (defined as S/N=3) ranging from 0.02 to 0.05 microg/mL for all analytes. This method was applied for the determination of the above four flavonoids in H. cordata Thunb. and S. chinensis (Lour.) Bail. with simple extraction procedures, and the assay results were satisfactory. Determination of flavonoids in Houttuynia cordata Thunb. and Saururus chinensis (Lour.) Bail. by capillary electrophoresis with electrochemical detection. Xu X, Ye H, Wang W, Yu L, Chen G No
18973011 OBJECTIVE: To study the chemical constituents of Rhizoma Cyperi.METHODS: The constituents were separated and purified by silica gel column chromatography, their structures were identified on the basis of physico-chemical properties and spectral data.RESULTS: Six compounds were isolated and identified as physicion (1), hexadecanoic acid (2), beta-sitosterol (3), stigmasterol (4), catenarin (5), daucosterol (6).CONCLUSION: Compounds 1, 4, 5 were isolated from this plant for the first fime. [Study on the chemical constituents of Rhizoma Cyperi]. Wu X, Xia HL, Huang LH, Chen DD, Chen JY, Weng HT. No
18985282 Two new phenolic glycosides-3'-O-beta-D: -glucopyranosysalidroside (1) and cis-echinacoside (2)-together with four known ones-forsythoside B (3), decaffeoylacteoside (4), osmanthuside F (5) and (-)-olivil-4'-O-beta-D: -glucopyranoside (6)-were isolated from the leaves of Syringa reticulata. Their structures were established on the basis of spectral and chemical data. Two new phenolic glycosides from Syringa reticulata. Machida K, Ohkawa N, Ohsawa A, Kikuchi M. No
18997392 Eight compounds were isolated from the roots of Garcinia polyantha, and identified. Two of them, the xanthone garciniaxanthone I (1), and the triterpene, named garcinane (2), are reported as new natural products. The structures of the new compounds were elucidated on the basis of 1D and 2D NMR spectroscopic studies. The structure of compound 1 was confirmed by X-ray crystallography. Among the remaining six known compounds, three were known xanthones [smeathxanthone A (3), smeathxanthone B (4), and chefouxanthone (5)], one benzophenone [isoxanthochymol (6)], one triterpene [magnificol], and one sterol [beta-sitosterol]. The in vitro antimalarial activity of isoxanthochymol (6) against Plasmodium falciparum shows strong chemosuppression of parasitic growth. Antimalarial compounds from the root bark of Garcinia polyantha Olv. Lannang AM, Louh GN, Lontsi D, Specht S, Sarite SR, Flörke U, Hussain H, Hoerauf A, Krohn K. No
18998397 Bioactivity-guided fractionation of Polygala alpestris L. (Rchb.) extracts led to the identification of two new xanthones, 1,3,7-trihydroxy-2,6-dimethoxyxanthone (1) and 2,3-methylenedioxy-4,7-dihydroxyxanthone (2). In addition five known compounds 3,4-dimethoxy-1,7-dihydroxyxanthone (3), 1,3-dihydroxy-7-methoxyxanthone (4), 1,7-dihydroxy-2,3-dimethoxyxanthone (5), 3',6-O-disinapoyl sucrose (6) and 3',5'-dimethoxybiphenyl-4-olo (7) were isolated. The structures of the isolated compounds were established by means of high resolution mass spectrometry, mono- and bi-dimensional NMR spectroscopy. All isolated compounds were tested for cytotoxic activity against three tumor cell lines (LoVo, HL-60, K 562). Xanthones from Polygala alpestris (Rchb.). Dall'Acqua S, Viola G, Cappelletti EM, Innocenti G. No
18998564 Abstarct:"OBJECTIVE:To study on the main chemical components of essential oil from Fructus Canarii.METHODS:The essential oil from Fructus Canarii was extracted by steam-stilling and analyzed by GC-MS. The relative content of each component was determined by normalization method.RESULTS:121 compounds were separated and 65 compounds were identified, which weighed 91.25% of the total oil. The main chemical components of the oil were caryophilene (24.78%), (+/-)-2-methylene-6,6-dimethyl,-bicyclo [3.1.1]-heptane (13.51%), p-menth-1-en-8-ol (7.15%) and so on.CONCLUSION:This experiment has provided scientific foundation for further utilization of Fructus Canarii." [Study on chemical components of the essential oil from Fructus Canarii by GC-MS]. Tan SY, Yang XR, Yang J, Duan WJ. No
18998565 Abstarct:"OBJECTIVE:To analyse the compositions of essential oil from leaves of Discocleidion rufescens.METHODS:To isolate by steam distillation and analyze by GC-MS.RESULTS:37 compounds, representing 92.25% of total oil were indentified and their relative amount was determined.CONCLUSION:The oil is rich in Phytol (39.30%, n-Hexadecanoic acid (11.72%), (Z) -3-Teoadecen-5-yne (5.78%) and beta-Pinene (3.63%)." [Extraction and GC-MS analysis of essential oil from leaves of Discocleidion rufescens]. Jia XN, Zhang YY, Yang YZ, Wang JX. No
18998567 OBJECTIVE: To study the chemical constituents of leaves of Paulownia fortunei (Seem.) Hemsl.METHODS: The constituents were isolated by column chromatography and their structures were elucidated through spectroscopic analysis.RESULTS: The compounds were identified as mimulone (I), apigenin (II), luteolin (III), 2alpha, 3beta, 19beta-trihydroxyurs-28-O-beta-D-galactonopyranos ylester (anserinoside, IV), 3alpha-hydroxyl-ursolicacid (V), ursolicacid (VI), daucosterol (VII), beta-sitosterol (VIII).CONCLUSION: The compounds I - V are obtained from leaves of Paulownia fortunei (Seem.) Hemsl for the first time. [Chemical constituents from leaves of Paulownia fortunei]. Li XQ, Wu JL, Cao FH, Li C. No
19003612 Two new ent-labdane diterpenoid glycosides were isolated from the aerial parts of Andrographis paniculata. Their structures were elucidated as 3-O-beta-D-glucosyl-14-deoxyandrographiside (1) and 3-O-beta-D-glucosyl-14-deoxy-11,12-didehydroandrographiside (2) by means of 1D and 2D NMR spectral and chemical methods. Two new ent-labdane diterpenoid glycosides from the aerial parts of Andrographis paniculata. Zhou KL, Chen LX, Zhuang YL, Wang NL, Yao XS, Qiu F. No
19007011 OBJECTIVE: To study the chemical constituents of the stems of Clematis parviloba.METHOD: The compounds were isolated and purified by repeated column chromatography with silica gel, Sephadex LH-20 and HPLC. Their structures were identified by spectroscopic data together with physical and chemical property.RESULT: Ten compounds have been isolated from the stems of C. parviloba, and identified as: (+) pinoresionol (1), (+) pinoresionol-4'-O-p-D-glucopyranoside (2), ( +) pinoresionol4, 4'-O-bis-beta-D-glucopyranoside (3), (-) syringaresinol (4), (+) syringaresinol-4'-O-beta-D-glucopyranoside (5), (-)episyringaresinol (6), (+) medioresinol-4'-O-beta-D-glucopyranoside (7), (+) lariciresinol-4-O-beta-D-glucopyranoside (8), (+) lariciresinol-4'-O-beta-D-glucopyranoside (9), (+) lariciresinol-4, 4'-O-bis-beta-D-glucopyranoside (10), respectively.CONCLUSION: Compounds 6, 7 were isolated from this genus for the first time, and the other ones were isolated from this plant for the first time. [Studies on lignan constituents of Clematis parviloba]. Yan LH, Xu LZ, Lin J, Zou ZM, Zhao BH, Yang SL. No
19038060 Abstarct:"BACKGROUND:Eight medicinal plants were tested for their antimicrobial and antioxidant activities. Different extraction methods were also tested for their effects on the bioactivities of the medicinal plants.METHODS:Eight plants, namely Herba Polygonis Hydropiperis (Laliaocao), Folium Murraya Koenigii (Jialiye), Rhizoma Arachis Hypogea (Huashenggen), Herba Houttuyniae (Yuxingcao), Epipremnum pinnatum (Pashulong), Rhizoma Typhonium Flagelliforme (Laoshuyu), Cortex Magnoliae Officinalis (Houpo) and Rhizoma Imperatae (Baimaogen) were investigated for their potential antimicrobial and antioxidant properties.RESULTS:Extracts of Cortex Magnoliae Officinalis had the strongest activities against M. Smegmatis, C. albicans, B. subtilis and S. aureus. Boiled extracts of Cortex Magnoliae Officinalis, Folium Murraya Koenigii, Herba Polygonis Hydropiperis and Herba Houttuyniae demonstrated greater antioxidant activities than other tested medicinal plants.CONCLUSION:Among the eight tested medicinal plants, Cortex Magnoliae Officinalis showed the highest antimicrobial and antioxidant activities. Different methods of extraction yield different spectra of bioactivities." Antimicrobial and antioxidant activities of Cortex Magnoliae Officinalis and some other medicinal plants commonly used in South-East Asia Chan LW, Cheah EL, Saw CL, Weng W, Heng PW. PMC2631514
19048786 To study xanthones from the barks of Garcinia xanthochymus, the constituents were isolated by normal-phase and reverse-phase silica gel column chromatography from the EtOAc extract. Their structures were elucidated by spectral analysis. Three new xanthones were purified and identified as 1,2,5-trihydroxy-6-methoxyxanthone (1), 1,4,6-trihydroxy-5-methoxyxanthone (2), 1,2,7-trihydroxy-4-(1,1-dimethylallyl) xanthone (3). Three new xanthones from Garcinia xanthochymus. Zhong FF, Chen Y, Song FJ, Yang GZ. No
19053509 Species of Swietenia elaborate limonoid chemistry along only one route, which leads to the mexicanolide type in most species and the phragmalin type in S. mahogani. A hexane extract from leaves of S. macrophylla afforded six new phragmalins with a 8,9,30-ortho-ester unit, namely, 6-O-acetylswietephragmin E (1), 3beta-O-destigloyl-3beta-O-benzoyl-6-O-acetylswietephragmin E (2), 12alpha-acetoxyswietephragmin C (3), 3beta-O-destigloyl-3beta-O-benzoyl-12alpha-acetoxyswietephragmin C (4), 12alpha-acetoxyswietephragmin D (5), and 3beta-O-destigloyl-3beta-O-benzoyl-12alpha-acetoxyswietephragmin D (6). This appears to be the first record of phragamalins from S. macrophylla, and this study shows the potential of tricyclic [3.3.1(2,10).1(1,4)]-decane limonoids as taxonomically useful chemical markers in the Meliaceae. Limonoids of the phragmalin type from Swietenia macrophylla and their chemotaxonomic significance. da Silva MN, Arruda MS, Castro KC, da Silva MF, Fernandes JB, Vieira PC. No
19085005 Mosquito control is facing a threat due to the emergence of resistance to synthetic insecticides. Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. The acetone, chloroform, ethyl acetate, hexane, methanol and petroleum ether extracts of leaf, flower and seed of Cassia auriculata L., Leucas aspera (Willd.), Rhinacanthus nasutus KURZ., Solanum torvum Swartz and Vitex negundo Linn. were tested against fourth instar larvae of malaria vector, Anopheles subpictus Grassi and Japanese encephalitis vector, Culex tritaeniorhynchus Giles (Diptera: Culicidae). The larval mortality was observed after 24 h of exposure. All extracts showed moderate larvicidal effects; however, the highest mortality was found in leaf petroleum ether, flower methanol extracts of C. auriculata, flower methanol extracts of L. aspera and R. nasutus, leaf and seed methanol extracts of S. torvum and leaf hexane extract of V. negundo against the larvae of A. subpictus (LC(50) = 44.21, 44.69, 53.16, 41.07, 35.32, 28.90 and 44.40 ppm; LC(90) = 187.31, 188.29, 233.18, 142.66, 151.60, 121.05 and 192.11 ppm, respectively) and against the larvae of C. tritaeniorhynchus (LC(50) = 69.83, 51.29, 81.24, 71.79, 44.42, 84.47 and 65.35 ppm; LC(90) = 335.26, 245.63, 300.45, 361.83, 185.09, 351.41 and 302.42 ppm, respectively). These results suggest that the leaf petroleum ether, flower methanol extracts of C. auriculata, leaf and seed methanol extracts of S. torvum and leaf hexane extract of V. negundo have the potential to be used as an ideal eco-friendly approach for the control of the A. subpictus and C. tritaeniorhynchus. This is the first report on the mosquito larvicidal activity of the medicinal plant extracts. Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G. No
19086633 OBJECTIVE: To study the antioxidant constituents from the roots of Securidaca inappendiculata.METHOD: The bioassay-guided isolation of antioxidant constituents was carried out by the column chromatographic techniques. The combination of IR, MS, NMR and 2D-NMR spectroscopics methods was used to identify their structures. Result: Two new xanthones, 1, 2, 5-trihydroxy-6, 8-dimethoxy-9H-xanthen-9-one(1), 1, 5-dihydroxy-2, 6, 8-trimethoxy-9H-xanthen-9-one (2), along with seven known ones, 3, 8-dihydroxy-1, 4-dimethoxy-9H-xanthen-9-one(3), 4, 6-dihydroxy-1, 5, 7-trimethoxy-9H-xanthen-9-one(4), 7-hydroxy-1, 2, 3, 8-tetramethoxy-9H-xanthen- 9-one(5), 1, 7-dihydroxy-9H-xanthen-9-one(6), 4-hydroxy-3, 7-dimethoxy-9H-xanthen-9-one(7), 1,7-dimethoxy-9H-xanthen-9-one(8) and aucuparin(9), were isolated from the roots of S. inappendiculata.CONCLUSION: Compounds 1 and 2 were new xanthones, and compound 3 was isolated as a natural product for the first time, and compounds 4 and 6 were isolated for the first time from this genus. The antioxidant activities of all compounds were evaluated by ABTS, FRAP and DPPH assays respectively. Compound 9 showed significant activity by the ABTS and FRAP assays. Compound 1 showed significant activity with IC50 value of 0.31 mg x L(-1) in DPPH assay. Scavenging capacity of all compounds determined by all assays were well correlated between ABTS and FRAP assay (r = 0.9555). [Antioxidant xanthones from Securidaca inappendiculata]. Kang WY, Li CF, Song YL. No
19086637 OBJECTIVE: To study the chemical constituents from the roots of Pterospermum heterophyllum.METHOD: The chemical constituents of P. heterophyllum were isolated and purified by silica gel and sephadex LH-20 column chromatography. Their structures were identified on the basis of spectroscopic data and physicochemical properties.RESULT: Nine compounds were isolated and identified as taraxerol (1), betulin (2), betulinic acid (3), sumaresinolic acid (4), 2-methoxy-5-hydroxy-1, 4-naphthoquinone (5), 5, 7-dihydroxy-6, 8-dimethylchromone (6), alpha-monpalmitin (7), palmitic acid (8), beta-sitosterol (9).CONCLUSION: Compounds 2-7 were isolated from this genus for the first time. [Studies on chemical constituents from roots of Pterospermum heterophyllum]. Shi Y, Li S, Li HY, Cui BS, Yuan Y. No
19099464 Three new Daphniphyllum alkaloids, calyciphyllines N-P (1-3), were isolated from the leaves and stems of Daphniphyllum calycinum. The structures and relative stereochemistry of 1-3 were elucidated on the basis of spectroscopic data. Calyciphyllines N-P, alkaloids from Daphniphyllum calycinum. Yahata H1, Kubota T, Kobayashi J. No
19107824 From the whole plant of Gentianopsis paludosa seven compounds were isolated and identified to be 1,7-dihydroxy-3,8-dimethoxyxanthone (1), 1,7,8-trihydroxy-3-methoxyxanthone (2), 1-hydroxy-3,7,8-trimethoxyxanthone (3), 1,8-dihydroxy-2,6-dimethoxyxanthone (4), oleanolic acid (5), 4',5,7-trihydroxyflavone (6) and luteolin-7-O-glucoside (7). Compounds 1, 2, 3 and 5 showed modest inhibitory effect against the growth of Mycobacterium smegmatis and M. tuberculosis. Search for antimycobacterial constituents from a Tibetan medicinal plant, Gentianopsis paludosa. Yeung MF, Lau CB, Chan RC, Zong Y, Che CT. No
19110407 Fagara zanthoxyloides Lam. (syn. Zanthoxylum zanthoxyloides) (Rutaceae) is the most cited Fagara species for the treatment and the prevention of sickle cell disease crisis. Sickle cell anemia (SCA) is a public health problem in many countries particularly in Africa. The present study was designed to evaluate the antisickling properties of three isomeric divanilloylquinic acids (3,4-O-divanilloylquinic acid or burkinabin A; 3,5-O-divanilloylquinic acid or burkinabin B and 4,5-O-divanilloylquinic acid or burkinabin C) identified previously by LC/MS/NMR analysis in the root bark of F. zanthoxyloides [Ouattara et al., 2004. LC/MS/NMR analysis of isomeric divanilloylquinic acids from the root bark of Fagara zanthoxyloides Lam. Phytochemistry 65, 1145-1151]. The three isomers showed interesting antisickling properties which increased from burkinabins A to C. Antisickling properties of divanilloylquinic acids isolated from Fagara zanthoxyloides Lam. (Rutaceae). Ouattara B1, Jansen O, Angenot L, Guissou IP, Frédérich M, Fondu P, Tits M. No
19157124 OBJECTIVE: To study the chemical constituents in the aerial part of Reineckea carnea.METHOD: The compounds were isolated by extraction, silica gel, gel, and reversed-phase silica gel coloum chromatography, and high-performance liquid chromatography. The structures were identified by various spectroscopic methods including 1D and 2D NMR spectrum, MS, IR, etc.RESULT: Six compounds were isolated and identified as 1alpha, 3beta-dihydroxy-5beta-pregn-16-en-20-one-3-O-beta-D-glucopyranoside (1), syringaresinol-beta-D-glucoside (2), sophoraflavone B (3), stigmast-5, 22-dien-3-O-beta-D-glucopyranoside (4), daucosterol (5), a-D-glucose (6).CONCLUSION: Compound 1 was a new compound, coumpounds 2-6 were obtained from the plant for the first time. [Chemical constituents in aerial part of Reineckea carnea]. Xu X, Fu HZ. No
19157125 OBJECTIVE: To study the chemical constituents of the barks of Garcinia tetralata.METHOD: Compounds were isolated and purified repeatedly by silica gel and ODS column chromatography and their chemical structures were elucidated by their physicochemical properties and spectral data analysis.RESULT: Ten compounds were obtained and identified as 1, 7-dihydroxyxanthone (1), buchanaxanthone (2), garciniaxanthone H (3), 1, 2, 5-trihydroxyxanthone (4), subelliptenone H (5), 6-desoxyjacareubin (6), 6-deoxyisojacreubin (7), subelliptenone G (8), methyl orsellinate (9) and beta-sitosterol (10).CONCLUSION: Compounds 1-10 were isolated from this plant and compound 9 was obtained from the genus Garcinia for the first time. [Chemical constituents from barks of Garcinia tetralata]. Wang LL, Li ZL, Hua HM, Liu XQ. No
19161345 From the whole herbs of Delphinium majus, three new C(19)-diterpenoid alkaloids, majusines A-C (1-3), and six new C(20)-diterpenoid alkaloids, majusimines A-D (4-7) and majusidine A and B (8 and 9), have been isolated, together with 15 known compounds. The structures of compounds 1-9 were elucidated by spectroscopic data interpretation. Diterpenoid Alkaloids from Delphinium majus. Chen FZ, Chen DL, Chen QH, Wang FP. No
19166007 OBJECTIVE: To investigate the chemical constituents of the aerial parts of Ammopiptanthus mongolicus.METHOD: The chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data.RESULT: Ten compounds were isolated and identified as m-hydroxybenzoic acid (1), 1-(4-hydroxyphenyl) ethanone (2), beta-sitosterol (3), (-)-syringaresinol (4), (+)-lariciresinol (5), blumenol A (6), blumenol B (7), beta-daucosterol (8), coniferin (9), syringin (10).CONCLUSION: The ten compounds were obtained from the genus Ammopiptanthus for the first time. [Studies on chemical constituents of aerial parts of Ammopiptanthus mongolicus]. Tian XM, Chen SZ, Tu PF, Lei LD. No
19180950 OBJECTIVE: To study the chemical constituents of Solanum cathayanum.METHODS: Chemical constituents were isolated by chromatographic methods and identified by NMR analysis.RESULTS: Five compounds were isolated and identified as lignoceric acid (I), beta-sitosterol (II), lupeol (III) 8-hydroxy-3-methoxy-5H-pyrido [2, 1-c] pyrazin-5-one (IV), ethly 2,6-dioxo-1,2,3,6-tetrahy-dropyrimidine-4-carboxylate (V).CONCLUSION: Compound I and III are isolated from this plant for the first time. Compound IV and V are isolated from the genus of Solanum for the first time. [Chemical constituents of Solanum cathayanum]. Xie MX, Zhou Y, Zou K, Cheng F, Liu R. No
19180955 OBJECTIVE: To study the chemical constituents of Salacia prinoides.METHODS: The chemical constituents were isolated and purified with various column chrmoatography and the structures were elucidated by chemical evidence and spectral analysis.RESULTS: Sixteen compounds,including seven triterpenes : lupeol (1), Lup-20(29)-en-3beta,30-diol (2), 30-Hydorxylup-20 (29) -en-3-one (3), 3, 22-dioxo-29-normoretane (4), Ursolid acid (5), beta-Sitosterol (6), beta-Daucosterol (7); Four flavanoids: Quercetin (8), Quercet-in-3', 4'-dimethylether (9), Isorhamnetin (10), Kaempferol-4'-methylether (11); Three Phenolic acids:Gallic acid (12), Ethyl gallate (13), Egallic acid (14); Two Fatty series: Hentriacontanol (15), Hentriacontan-12-ol (16) were obtained from the titled plant.CONCLUSION: -4, 8 - 13 are isolated from the genus for the first time, 5, 14 -16 are isolated from the plant for the first time. [Studies on chemical constituents of Salacia prinoides]. Gao XH, Xie N, Feng F. No
19184276 Five new glycosides, quercetin 3'-O-beta-D: -galactopyranoside (1), quercetin 3-O-(2''-acetyl)-beta-D: -glucopyranoside (2), 4,6-dihydroxy-2-methoxyphenyl 1-O-beta-D: -glucopyranoside (3), 4-hydroxy-2,6-dimethoxyphenyl 1-O-alpha-L: -rhamnopyranosyl (1 --> 6)-beta-D: -glucopyranoside (4) and 3-methyl-but-2-en-1-yl beta-D: -glucopyranosyl (1 --> 6)-beta-D: -glucopyranoside (5), were isolated from Hypericum erectum Thunb. Their structures were established on the basis of spectral and chemical data. Five new glycosides from Hypericum erectum Thunb. Machida K, Matsuoka E, Kikuchi M. No
19191551 Four new alkaloids, alstiphyllanines A-D (1-4), were isolated from Alstonia macrophylla, and their structures were determined by MS and 2D NMR analyses. Alkaloids 1-4 showed moderate antiplasmodial activity against Plasmodium falciparum and vasorelaxant activity against phenylephrine-induced contraction of isolated rat aorta. Alstiphyllanines A-D, indole alkaloids from Alstonia macrophylla. Hirasawa Y1, Arai H, Zaima K, Oktarina R, Rahman A, Ekasari W, Widyawaruyanti A, Indrayanto G, Zaini NC, Morita H. No
19205264 The antimicrobial activities of aqueous and organic solvents (chloroform, ethanol and methanol) extracts of four plants Ceratophyllum demersum L., Eichhornia crassipes, Potamogeton crispus and Potamogeton pectinatus were tested in vitro against seventeen different microorganisms including Gram-positive and Gram-negative bacteria and fungi. Nine of these identified organisms were obtained from different sources, Bacillus subtilis 1020, Bacillus cereus 1080, Staphylococcus aureus, Erwinia carotovora NCPPB 312, Candida albicans, Candida tropicalis, Aspergillus niger, Fusarium oxysporum and Penicillium italicum. The other eight organisms were isolated from Manzalah lake water and identified using API 20E strip system (BioMereux). One hundred pathogenic bacterial isolates representing eight genera were identified to species level. These organisms are Escherichia coli (20%), Pseudomonas aeruginosa (16%), Klebsiella pneumoniae (14%), Salmonella colerasuis (13%), Shigella sp. (11%), Serratia liquefaciens (10%), Proteus vulgaris (9%) and Brenneria nigrifluens (7%). The extracts of all tested plants demonstrated antimicrobial activity against the used organisms. The efficiency of the extracts varied with, solvent used in the extraction as well as plant species and the part of plant used. The aqueous extract appeared to be the highly effective extract against all tested organisms especially Fusarium oxysporum causing inhibition zone 48 +/- 0.01 mm, Pseudomonas aeruginosa 59 +/- 0.02 mm and Salmonella cholerasuis 55 +/- 0.01 mm when using P. crispus, P. pectinatus and C. demersum, respectively. Ethanol extracts of C. demersum, P. crispus and E. crassipes root showed antimicrobial activities against all tested organisms except Aspergillus niger. At the same time the extract of P. pectinatus had no effect also on Fusarium oxysporum and the extract of E. crassipes leaves have no effect on Penicillium italicum. On using chloroform extracts Escherichia coli, Aspergillus niger and Penicillium italicum showed resistance. Comparing the effect of different plants extracts C. demersum appeared to be the most effective followed by P. pectinatus. Furthermore, the extracts of E. crassipes leaves being more effective than that, of its roots. Elemental analysis were also takes place in water and plant samples and the results revealed the presence of Mn and Pb in higher concentration in P. pectinatus (Mn 603 +/- 4.243 ppm and Pb 44 +/- 2.828 ppm), at the same time the highest values of Fe 1680 +/- 2.2 ppm, Zn 31.5 +/- 2.1 ppm and Cu 26.5 +/- 2.1 ppm were recorded for C. demersum. Comparing the two parts of E. crassipes (leaves and roots), the roots have the highest values of all studied metals. Antimicrobial activity of some macrophytes from Lake Manzalah (Egypt). Fareed MF, Haroon AM, Rabeh SA. No
19217125 Two seco-tabersonine alkaloids, jerantiphyllines A and B, in addition to a tabersonine hydroxyindolenine, jerantinine H, and a recently reported vincamine alkaloid 7, were isolated from the leaf extract of the Malayan Tabernaemontana corymbosa and the structures were established using NMR and MS analysis. Biomimetic conversion of jerantinines A and E to their respective vincamine and 16-epivincamine derivatives were also carried out. Seco-tabersonine alkaloids from Tabernaemontana corymbosa. Lim KH1, Thomas NF, Abdullah Z, Kam TS. No
19230398 OBJECTIVE: To investigate the chemical constituents of Ipomoea batatas.METHODS: Silica gel column chromatography and Sephadex LH-20 were used to separate and purify the compounds from the EtOAc and n-BuOH soluble fraction of ethanol extracts. The chemical structures were elucidated on the basis of physic-chemical properties and spectral data.RESULTS: Four compounds were isolated and identify as citrusin C (1), caffeicacid (2), 3,4-di-O-caffeoylquinic acid (3), 1,2,3,4-tetrahydro-beta-carboline-3-carboylic acid (4).CONCLUSION: Compounds 1-4 are isolated for the first time from I. batatas. [Studies on chemical constituents from Ipomoea batatas]. Yin YQ, Shen ZB, Kong LY. No
19230400 OBJECTIVE: To investigate the chemical constituents of Grewia biloba and find its bioactive compounds.METHODS: Compounds were isolated by silica gel, MCI, Sephadex LH-20 and Rp-18 column chromatography, and purified by recrystallization. Their structures were elucidated by spectral analysis and other methods.RESULTS: Six compounds were isolated and identified as friedelin (1) epi-friedelan-3-ol (2), heneicosanoic acid (3), beta-sitosterol (4), Propyl palmitate (5), eatechin (6), respectively.CONCLUSION: Compounds 1-3, 5, 6 are isolated from Grewia genus for the first time and compounds 4 is isolated from Grewia biloba for the first time. [Studies on chemical constituents from Grewia biloba]. Liu JQ, Wu JM, Kou XL, Hong Q. No
19235027 The essential oil of the aerial parts of the endemic Achillea umbellata (Compositae) was obtained by hydrodistillation and analysed by gas chromatography (GC) and gas chromatography coupled to mass spectrometry (GC-MS). Sixty-six constituents were identified. The major constituents of the oil were beta-thujone (62.8%) and camphor (8.7%). Chemical composition of the essential oil of Achillea umbellata growing in Greece. Tzakou O, Loukis A. No
19245240 Lycojapodine A, a novel C(16)N-type Lycopodium alkaloid with an unprecedented 6/6/6/7 tetracyclic ring system, was isolated from the club moss Lycopodium japonicum. The structure and relative stereochemistry were elucidated on the basis of spectroscopic data and were further confirmed by X-ray analysis. A possible biosynthetic pathway for 1 was proposed. Its inhibitory activity on acetylcholinestrease and anti-HIV-1 activity were also evaluated. Lycojapodine A, a novel alkaloid from Lycopodium japonicum. He J, Chen XQ, Li MM, Zhao Y, Xu G, Cheng X, Peng LY, Xie MJ, Zheng YT, Wang YP, Zhao QS. No
19260270 Abstarct:"OBJECTIVE:The chemical components of essential oil from Magnolia biondii were analyzed by GC-MS.METHODS:Essential oil was extracted by steam distillation (SD). The chemical components of essential oil were analyzed by GC-MS.RESULTS:The chemical components in the oil were qualitatively and quantitatively analyzed by GC-MS. 63 components were separated and 50 components were identified. The main components were Eucalyptol (28.92%), P-pinene (12.39%), alpha-Terpineol (8.28%).CONCLUSION:This is the first time to adopt GC-MS to analyze the chemical components of volatile oil of Magnolia biondii, and this study can provide science basis for further research development of Magnolia biondii." [Analysis of the chemical constituents of essential oil from Magnolia biondii by GC-MS]. Lu JQ, Xu YT, Zhan XL, Wan W, He XF, Wu DH, Cao RB, Hu XY, Yang XH. No
19260277 OBJECTIVE: To investigate the chemical constituents of root of Rubus biflorus. METHODS: Isolation and purification were carried out by silica gel column chromatography and recrystallization and so on. Structural determination of the pure compounds was based on physico-chemical properties and various spectral data analysis (1H-NMR, 13C-NMR, IR, EI-MS). RESULTS: Five compounds were isolated and identified as follows: lupeol acetate (I), corosolic acid (II), oleanic acid (III), 2alpha-hydroxyoleanolic acid (IV), sitosterol (V). CONCLUSION: All these compounds are isolated from this plant for the first time. [Triterpenoids and steroids of root of Rubus biflorus]. Kang SH, Shi YQ, Yang CX. No
19266907 Chemical constituents of the bark of Swietenia macrophylla King (Meliaceae) was investigated not only to develop further bark utilization but also to understand the biochemical function of the bark in the forest environment. A new phenylpropanoid-substituted catechin, namely, swietemacrophyllanin [(2R*,3S*,7"R*)-catechin-8,7"-7,2"-epoxy-(methyl 4",5"-dihydroxyphenylpropanoate)] (1) was isolated from the bark of S. macrophylla together with two known compounds, catechin (2) and epicatechin (3). The structure of 1 was elucidated by spectroscopic data and by comparison of the NMR data with those of catiguanins A and B, phenylpropanoid-substituted epicatechins. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity of the isolated compounds indicated that all of the three compounds have strong activity compared with trolox as a reference. Swietemacrophyllanin (1) had the strongest activity with a 50% inhibitory concentration (IC50) value of 56 microg mL(-1). Chemical constituents from Swietenia macrophylla bark and their antioxidant activity. Falah S, Suzuki T, Katayama T. No
19296616 Four new xanthones, butyraxanthones A-D (1-4), were isolated from the stem bark of Pentadesma butyracea, together with six known xanthones (5-10) and a triterpenoid (lupeol). The structures of 1-4 were established by spectroscopic methods. Compounds 1-10 were tested in vitro for antiplasmodial activity against a Plasmodium falciparum chloroquine-resistant strain and for cytotoxicity against a human breast cancer cell line (MCF-7). Nearly all of these xanthones exhibited good antiplasmodial activity, and some of them also demonstrated potent cytotoxicity. Cytotoxic and antiplasmodial xanthones from Pentadesma butyracea. Zelefack F, Guilet D, Fabre N, Bayet C, Chevalley S, Ngouela S, Lenta BN, Valentin A, Tsamo E, Dijoux-Franca MG. No
19320966 Abstarct:"BACKGROUND:Recent years have witnessed that there is a revival of interest in drug discovery from medicinal plants for the maintenance of health in all parts of the world. The aim of this work was to investigate 26 plants belonging to 17 families collected from a unique place in Yemen (Soqotra Island) for their in vitro anticancer, antimicrobial and antioxidant activities.METHODS:The 26 plants were extracted with methanol and hot water to yield 52 extracts. Evaluation for in vitro anticancer activity was done against three human cancer cell lines (A-427, 5637 and MCF-7) by using an established microtiter plate assay based on cellular staining with crystal violet. Antimicrobial activity was tested against three Gram-positive bacteria, two Gram-negative bacteria, one yeast species and three multiresistant Staphylococcus strains by using an agar diffusion method and the determination of MIC against three Gram-positive bacteria with the broth micro-dilution assay. Antioxidant activity was investigated by measuring the scavenging activity of the DPPH radical. Moreover, a phytochemical screening of the methanolic extracts was done.RESULTS:Notable cancer cell growth inhibition was observed for extracts from Ballochia atro-virgata, Eureiandra balfourii and Hypoestes pubescens, with IC50 values ranging between 0.8 and 8.2 microg/ml. The methanol extracts of Acanthospermum hispidum, Boswellia dioscorides, Boswellia socotrana, Commiphora ornifolia and Euphorbia socotrana also showed noticeable antiproliferative potency with IC50 values < 50 microg/ml. The greatest antimicrobial activity was exhibited by extracts from Acacia pennivenia, Boswellia dioscorides, Boswellia socotrana, Commiphora ornifolia, Euclea divinorum, Euphorbia socotrana, Leucas samhaensis, Leucas virgata, Rhus thyrsiflora, and Teucrium sokotranum with inhibition zones > 15 mm and MIC values <or= 250 microg/ml. In addition, the methanolic extracts of Acacia pennivenia, Boswellia dioscorides, Boswellia socotrana and Commiphora ornifolia showed good antioxidant potential at low concentrations (more than 80% at 50 microg/ml).CONCLUSION:Our results show once again that medicinal plants can be promising sources of natural products with potential anticancer, antimicrobial and antioxidative activity. The results will guide the selection of some plant species for further pharmacological and phytochemical investigations." Studies of the in vitro anticancer, antimicrobial and antioxidant potentials of selected Yemeni medicinal plants from the island Soqotra. Mothana RA, Lindequist U, Gruenert R, Bednarski PJ.
PMC2667473
19368377 A new 10-membered ring meroterpene (1), named globiferin, was isolated from root extracts of Cordia globifera. Biomimetic transformations of 1 and its derivatives, either by acid cyclization or by Cope rearrangement, provided information relating to the biogenesis of cordiachromes A-C. Globiferin (1) underwent Cope rearrangement upon refluxing in xylene and DMSO-d(6) to yield cordiachrome C (3) and cordiaquinol C (4), respectively. Heating in DMSO-d(6) resulted in an unexpected reduction of a quinone moiety. Globiferin diacetate (1b) cyclized under acidic conditions to give compounds 10 and 11, respective derivatives of natural cordiachromes B (2) and A (12). The present study indicates that globiferin (1) is a genuine intermediate for the biosynthesis of cordiachromes in Cordia species. Compounds 1 and 3 exhibited significant antimycobacterial activity, with MIC values of 6.2 and 1.5 mug/mL, respectively. Antimalarial, antifungal, and cytotoxic activities of 1 and its derivatives were also evaluated. Biomimetic transformation and biological activities of Globiferin, a terpenoid benzoquinone from Cordia globifera. Dettrakul S1, Surerum S, Rajviroongit S, Kittakoop P. No
19370886 The essential oil from the aerial parts of Stachys cretica L. subsp. smyrnaea Rech. fil. (Lamiaceae), endemic to Turkey, was investigated by using GC and GC-MS. Thirty-four of 37 components, represented 99.7% of the total oil, were identified. The major components of the essential oil were trans-beta-caryophyllene (51.0%), germacrene-D (32.8%), a-humulene (3.1%), delta-cadinene (2.1%) and delta-elemene (2.1%). The antimicrobial activity of the essential oil, trans-beta-caryophyllene and five different extracts of the aerial parts of S. cretica L. subsp. smyrnaea were investigated by the standard disc diffusion method. The essential oil and trans-beta-caryophyllene exhibited antibacterial and antifungal activities. The activity increased with increasing concentrations of the essential oil and the extracts. The essential oil showed antimicrobial activity, particularly against Pseudomonas aeruginosa and Bacillus subtilis. The extracts exhibited either moderate or no activity. GC-MS analysis and antimicrobial activity of essential oil of Stachys cretica subsp. smyrnaea. Oztürk M, Duru ME, Aydoğmuş-Oztürk F, Harmandar M, Mahliçli M, Kolak U, Ulubelen A. No
19370887 The essential oils of the aerial parts and fruits of Chaerophyllum aureum L., collected from two mountains in Serbia, were analyzed by GC and GC/MS. Sabinene (18.5-31.6%), p-cymene (7.9-25.4%) and limonene (1.9-10.9%) were characterized as the main constituents. The oils were tested against six bacterial strains and one strain of yeast, Candida albicans. The highest antimicrobial activity was observed against the Gram-positive bacteria Staphylococcus aureus, S. epidermidis and Micrococcus luteus, while of the Gram-negative strains, Escherichia coli was the most sensitive. Lakusić B, Slavkovska V, Pavlović M, Milenković M, Stanković JA, Couladis M. Chemical composition and antimicrobial activity of the essential oil from Chaerophyllum aureum L. (Apiaceae). No
19370888 The aerial parts of Tanacetum balsamita subsp. balsamita were collected at full flowering stage from Tabriz, Iran. The essential oil was isolated by hydrodistillation and analyzed by combination of capillary GC and GC-MS. Twenty seven components were identified, representing 99.2% of the total oil. Carvone (51.0%), beta-thujone (20.8%), 1,8-cineole (4.4%) and alpha-thujone (3.2%) were the main components. The in vitro antimicrobial activity of the essential oil of T. balsamita subsp. balsamita was studied against eight Gram-positive and Gram-negative bacteria (Bacillus subtilis, B. pumulis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The results of the antimicrobial activity of the essential oil according to the disc diffusion method and MIC values indicated that the oil exhibited moderate to high antimicrobial activity. The in vitro toxicological study based on the MTT cytotoxicity assay on human fetal skin fibroblast (HFSF) and Monkey kidney (Vero) cell lines showed IC50 values of 2500 and 1250 microg/mL, respectively. Cytotoxicity, antimicrobial activity and composition of essential oil from Tanacetum balsamita L. subsp. balsamita. Yousefzadi M, Ebrahimi SN, Sonboli A, Miraghasi F, Ghiasi S, Arman M, Mosaffa N. No
19385182 OBJECTIVE: To investigate the chemical constituents in the roots of Polygonum amplexicaule var. sinense.METHOD: The compounds were isolated by column chromatography on silica gel and Sephadex LH-20. The structures were identified by means of IR, 1H-NMR, 13C-NMR and MS analyses.RESULT: Eight compounds were isolated and identified as friedelin (1), beta-sitosterol (2), simiarenone (3), angelicin (4), psoralen (5), palmitic acid (6), (-)-epicatechin (7), and quercetin (8), respectively.CONCLUSION: All compounds were isolated from this species for the first time and compounds 1, 3-6 were obtained from this genus for the first time. [Studies on chemical constituents from roots of Polygonum amplexicaule]. Ren H, Wan D, Zou Z. No
19408151 Four new pentacyclic triterpene saponins, philoxeroidesides A-D (1-4) were isolated from the aerial parts of Alternanthera philoxeroides. Their structures were elucidated on the basis of 1D- and 2D-NMR experiments and MS analyses. Philoxeroidesides A-D (1-4) showed cytotoxic activities against SK-N-SH and HL60 cell lines. Cytotoxic triterpene saponins from Alternanthera philoxeroides. Fang JB, Yao Z, Chen JC, Liu YW, Takaishi Y, Duan HQ. No
19408685 To study the xanthones from the leaves of Calophyllum inophyllum Linn., several chromatography methods were employed to isolate the constituents. Investigation on the CHCl3 extract led to the isolation of a new xanthone named inophyxanthone A (1) and four known compounds, which were pancixanthone A (2), gerontoxanthone B (3), jacareubin (4) and pyranojacareubin (5). Among them, compound 2 was obtained from this plant firstly, and compound 3 was obtained for the first time from this genus. The structure of inophyxanthone A (1) was identified as 1, 3, 5-trihydroxy-2-(1, 1-dimethylallyl)xanthone by spectral analysis. [Xanthones from leaves of Calophyllum inophyllum Linn]. Li Y, Li ZL, Liu MS, Li DY, Zhang H, Hua HM. No
19409581 Iridoid glycosides, 2',3',6'-tri-O-acetyl-4'-O-trans-p-(O-beta-d-glucopyranosyl)coumaroyl-7-ketologanin (1), 2'-O-caffeoylloganic acid (2), 2'-O-p-hydroxybenzoylloganic acid (3), 2'-O-trans-p-coumaroylloganic acid (4), and 2'-O-cis-p-coumaroylloganic acid (5), were isolated from whole plants of Gentiana loureirii along with six known iridoids, 7-ketologanin (6), loganin (7), loganic acid (8), sweroside, boonein, and isoboonein, and three other known compounds. Their structures were elucidated by spectroscopic means and chemical correlations. The isolated iridoids were evaluated for antibacterial and antioxidant activities, but were either inactive or very weakly active. Iridoids from Gentiana loureirii. Wu M, Wu P, Liu M, Xie H, Jiang Y, Wei X. No
19413104 From the methanolic extract of the plant Hyptis verticillata Jacq, was isolated five compounds: the novel compounds cadina-10(15)-en-3-one and 3,7,11,15-tetramethylhexadec-1-en-3-ol and the known compounds 3,7,11,15-tetramethylhexadec-2-en-1-ol, 7,11,15-trimethyl-3-methylenehexadecane-1,2-diol and myo-inositol. All structures were established by spectroscopic means. Sesquiterpene and acyclic diterpenes from Hyptis verticillata Jacq. Biggs DA, Porter RB, Reynolds WF. No
19420781 Four novel prenylflavonols, macaranones A-D (1-4), were isolated from the leaves of Macaranga sampsonii. Their structures were elucidated on the basis of spectroscopic data. Macaranones C (3) and D (4) represent first two examples of flavonols having an unusual peltogynoid skeleton which is formed from a 2'-geranylflavonol by cyclization between 3-OH and C-1'' of the 2'-geranyl substituent of the flavonol. Compounds 1-4 were evaluated for the cytotoxicity against several human cancer cell lines. Prenylflavonols from the leaves of Macaranga sampsonii. Li X, Xu L, Wu P, Xie H, Huang Z, Ye W, Wei X. No
19420783 Three new Daphniphyllum alkaloids, daphnezomines T-V (1-3), were isolated from the leaves and branches of Daphniphyllum humile (Daphniphyllaceae). The structures and relative stereochemistry of 1-3 were elucidated on the basis of spectroscopic data. Daphnezomine T (1) is the first alkaloid without a branched C(1) unit at C-5 among all Daphiniphyllum alkaloids reported so far. Daphnezomines T--V, alkaloids from Daphniphyllum humile. Kubota T1, Suzuki T, Ishiuchi K, Kuhara T, Kobayashi J. No
19422205 Five new 7-O-methylkaempferol and -quercetin glycosides, namely, nervilifordins A-E (1-5), were isolated from the whole plant of Nervilia fordii, together with seven known flavonoids (6, 7, and 9-13) and one known coumarin (8). Their structures were elucidated on the basis of extensive spectroscopic analyses, including HSQC, HMBC, ROESY, and chemical methods. Compounds 1-3 and 6-13 were evaluated for their anti-herpes simplex virus 1 (HSV-1) activity and cytotoxicity on African green monkey kidney cells (Vero cells) in vitro. Of the tested compounds, only esculetin (8) exhibited antiviral activity against HSV-1, while the aglycones (11-13) showed stronger cytotoxicity on Vero cells than their glycosides (1-3, 6, and 7). 7-O-methylkaempferol and -quercetin glycosides from the whole plant of Nervilia fordii. Tian LW, Pei Y, Zhang YJ, Wang YF, Yang CR. No
19431011 Two new xanthones, 6-hydroxy-3,7-dimethoxy-8-(3-methylbut-2-enyl)-6',6'-dimethyl-5'-hydroxy-4',5'-dihydropyrano(2',3':1,2)xanthone (1) and 6-hydroxy-3,7-dimethoxy-8-(2-oxo-3-methylbut-3-enyl)-6',6'-dimethyl-5'-hydroxy-4',5'-dihydropyrano(2',3':1,2)xanthone (2), have been isolated from the stems of Cratoxylum cochinchinense (Lour.) Blume. Their structures were established on the basis of spectroscopic analysis. Two new xanthones from the stems of Cratoxylum cochinchinense. Jin SL, Wang NL, Zhang X, Dai Y, Yao XS. No
19441072 Three acylphloroglucinol derivatives have been isolated from the hexane and acetone extracts of the aerial parts of Hypericum densiflorum Pursch. The compounds were characterized by NMR spectroscopy and mass spectrometry and identified as 4-geranyloxy-2,6-dihydroxybenzophenone (1), 4-geranyloxy-1-(2-methylpropanoyl)- phloroglucinol (2) and 4-geranyloxy-1-(2-methylbutanoyl)-phloroglucinol (3). Compounds 1-3 were evaluated for in vitro cell proliferation inhibitory activity against human breast (MCF-7), lung (NCI H460), CNS (SF-268), stomach (AGS) and colon (HCT-116) tumor cell lines; antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA); inhibition of cyclooxygenase (COX-1 and -2) enzymes; and antioxidant activity in the lipid peroxidation (LPO) assay. All three compounds showed moderate to strong antitumor, antibacterial, antioxidant and inhibition of COX-2 activities. Also, this is the first reported occurrence of compound 3 in the Hypericum genus. Bioactive acylphloroglucinols from Hypericum densiflorum. Henry GE, Campbell MS, Zelinsky AA, Liu Y, Bowen-Forbes CS, Li L, Nair MG, Rowley DC, Seeram NP. No
19441852 Four new Daphniphyllum alkaloids, dapholdhamines A-D (1-4), were isolated from the leaves of Daphniphyllum oldhami. The structures and relative configurations of 1-4 were elucidated on the basis of spectroscopic data. Dapholdhamines A-D, alkaloids from Daphniphyllum oldhami. Zhang Y, Di YT, Mu SZ, Li CS, Zhang Q, Tan CJ, Zhang Z, Fang X, Hao XJ. No
19445122 OBJECTIVE: To study the chemical constituents from the aerial part of Herba Cirsii.METHODS: Compounds were isolated by various chromatographic techniques and identified by spectral data.RESULTS: Eight compounds were obtained. Their structures were characterized as (7R,8S)-dehydroconiferyl alcohol-8,5'-dehydroconiferyl aldehyde-9-O-beta-D-glucopyranoside(I),(7S,8R)-dihydro-dehydrodiconiferyl alcohol-9-O-beta-D-glucopyranoside(II), Isokaempferide-7-O-beta-D-glucopyranoside(III), Quercetin-3-O-beta-D-gluco-pyranoside (IV), Pectolinarin (V), Rutin(VI), Sucrose(VII), beta-sitosterol(VIII).CONCLUSION: Among these compounds, compounds I-IV are isolated from Herba Cirsii for the first time, Compounds first time. I and II as lignan compounds from this genus, are reported for the first time. [Study on chemical constituents of Herba Cirsii]. Meng YH, Wang QH, Jiang H, Yang BY, Kuang HX. No
19447614 Two new Lycopodium alkaloids, serratezomines D (1) and E (2), were isolated from the club moss Lycopodium serratum var. serratum. Serratezomine D (1) is a new lucidine-type alkaloid, while serratezomine E (2) is a new phlegmarane-type alkaloid. The structures and relative stereochemistry of 1 and 2 were elucidated on the basis of spectroscopic data. Serratezomine D (1) exhibited an inhibitory activity against acetylcholinesterase Serratezomines D and E, new Lycopodium alkaloids from Lycopodium serratum var. serratum. Kubota T, Yahata H, Yamamoto S, Hayashi S, Shibata T, Kobayashi J. No
19483344 Two new Amaryllidaceae alkaloids, named lycoranines A (1) and B (2), were isolated from the bulbs of Lycoris radiata. Their structures were elucidated on the basis of extensive spectroscopic analysis. Compound 2 was a new-type alkaloid, which provided a new insight into the biosynthesis of alkaloids in Amaryllidaceae plants. Two new amaryllidaceae alkaloids from the bulbs of Lycoris radiata. Wang L, Zhang XQ, Yin ZQ, Wang Y, Ye WC. No
19489592 Four new cycloartane triterpenoids, together with the known gardenolic acid B, were isolated from Kleinhovia hospita. The triterpenoids (1-3) contain a unique 21,23-diacetal side-chain, while compound 4 contains two alpha,beta-unsaturated ketone moieties. Their structures and relative configurations were determined by spectroscopic methods, including 2D NMR and IR. These compounds showed promising hepatoprotective effects on nitrofurantoin-induced cytotoxicity in human liver-derived Hep G2 cells. Cycloartane triterpenoids from Kleinhovia hospita. Gan LS, Ren G, Mo JX, Zhang XY, Yao W, Zhou CX. No
19499937 Four new spermidine glycosides, dracotanosides A-D (1-4), have been isolated from Dracocephalum tanguticum. These molecules represent the first spermidine glycosides from this plant genus. The structures, including absolute configurations, were determined by spectroscopic and chemical methods. The amide bond rotational barrier of aglycone 1a was calculated by density functional theory (DFT) computation. Dracotanosides A-D, spermidine glycosides from Dracocephalum tanguticum: structure and amide rotational barrier. Wang SQ1, Ren DM, Xiang F, Wang XN, Zhu CJ, Yuan HQ, Sun LM, Lv BB, Sun XJ, Lou HX. No
19504390 Three new xanthones, 1,3-dihydroxy-2,5,6,7-tetramethoxyxanthone (1), 3-hydroxy-1,2,5,6,7-pentamethoxyxanthone (2), and 3,8-dihydroxy-1,2,6-trimethoxyxanthone (3), together with two known compounds were isolated from the roots of Polygala japonica Houtt. Their structures were determined on the basis of spectroscopic data. Three new xanthones from the roots of Polygala japonica Houtt. Xue QC, Li CJ, Zuo L, Yang JZ, Zhang DM. No
19508747 Leishmaniasis is a vector-borne disease caused by flagellated protozoan parasites of the genus Leishmania, which affects both humans and other mammals. Most of the available drugs against the disease are toxic and parasite resistance to some of the drugs has already developed. In the present study, the leishmanicidal activities of methanolic extracts of some Israeli plants have been evaluated in vitro, against the free-living promastigotes and intracellular amastigotes of Leishmania major. Of the 41 extracts examined, those of two plants (Nuphar lutea>Withania somnifera) were highly effective (with a maximum inhibitory effect of >50%), those of three other species (Pteris vittata>Smyrnium olusatrum>Trifolium clypeatum) were moderately effective (25%-50%) and another four extracts (Erodium malacoides>Hyparrhenia hirta>Thymelaea hirsuta>Pulicaria crispa) showed a marginal effect (15%-22%) against the parasites. Extracts of nine plant species therefore showed antileishmanial activity but only the extract of N. lutea, used at 1.25 microg/ml, eliminated all the intracellular parasites within 3 days of treatment, with no detectable toxicity to the host macrophages. The mean (S.D.) values recorded for the median inhibitory concentrations of this extract (IC50) against the promastigotes [2.0 (0.12) microg/ml] and amastigotes [0.65 (0.023) microg/ml] and the median lethal concentration (LD50) against macrophages [2.1 (0.096) microg/ml] were encouraging, giving a therapeutic selectivity index [LD50/IC50 for amastigotes)] of 3.23. The extract of N. lutea was, in fact, generally as effective as the paromomycin that was used as the 'gold standard' drug. These results indicate that N. lutea and probably also Withania somnifera might be potential sources of clinically useful, antileishmanial compounds. Antileishmanial activity in Israeli plants. El-On J, Ozer L, Gopas J, Sneir R, Enav H, Luft N, Davidov G, Golan-Goldhirsh A. No
19521919 The chemical constituents and biological activities of leaves and roots of Aloe hijazensis, collected in Saudi Arabia, are reported here for the first time. Twenty-two compounds were obtained, among them eight hydroxyquinones: aloe-emodin (1), emodin (2), chrysophanol (3), aloesaponarin II 3-methyl ether (4), ziganein (5), ziganein-5-methyl ether (6a), aloesaponarin I (7) and chrysophanein (8), the dihydro-isocoumarin feralolide (9), 4,7-dichloro-quinoline (10), the triterpene lupeol (11), the anthrone aloin (12), three aloenin derivatives, aloenin (13) ethylidene-aloenin (14), and aloenin B (15), four flavonoids, quercetin (16), kaempferol (17) cosmosiin (18) and isovitexin (19), and cinnamic acid (20) and two further analogues, caffeic acid (21) and ferulic acid (22). While 15 of the isolated compounds were found in the leaves, 12 were isolated from roots of the plant. Compounds 6a and 10 are reported as new natural constituents, while the compounds 4, 5, 8, and 18 are reported here for the first time from Aloe spp. The structures of the compounds were deduced by intensive studies of their UV, NMR, MS data and by comparison with related structures. The biological activity of plant extracts was studied against various microbial strains, and potent anti-bacterial and anti-fungal activities were found New bioactive compounds from Aloe hijazensis. Abd-Alla HI, Shaaban M, Shaaban KA, Abu-Gabal NS, Shalaby NM, Laatsch H. No
19523224 BACKGROUND: Mentha longifolia L. (Lamiaceae) leaves have been traditionally implemented in the treatment of minor sore throat and minor mouth or throat irritation by the indigenous people of Iraq, although the compounds responsible for the medicinal properties have not been identified. In the present study, an antimicrobial compound was isolated and characterized, and its biological activity was assessed. METHODS: The compound was isolated and characterized from the extracted essential oil using different spectral techniques: TLC, FTIR spectra and HPLC. Antimicrobial activity of the compound was assessed using both disc diffusion and microdilution method in 96 multi-well microtiter plates.RESULTS: A known compound was isolated from the essential oil of the plant and was identified as (-) menthol. The isolated compound was investigated for its antimicrobial activity against seven selected pathogenic and non-pathogenic microorganisms: Staphylococcus aureus, Streptococcus mutans, Streptococcus faecalis, Streptococcus pyogenis, Lactobacillus acidophilus, Pseudomonas aeruginosa and the yeast Candida albicans. Menthol at different concentrations (1:1, 1:5, 1:10, 1:20) was active against all tested bacteria except for P. aeruginosa, and the highest inhibitory effect was observed against S. mutans (zone of inhibition: 25.3 mm) using the disc diffusion method. Minimal inhibitory concentration MIC values ranged from 15.6-125.0 microg/ml, and the most promising results were observed against S. aureus and S. mutans (MIC 15.6 microg/ml) while, S. faecalis, S. pyogenis and L. acidophilus ranked next (MIC 31.2 microg/ml). Furthermore, menthol achieved considerable antifungal activity against the yeast C. albicans (zone of inhibition range: 7.1-18.5 mm; MIC: 125.0). CONCLUSION: The isolation of an antimicrobial compound from M. longifolia leaves validates the use of this plant in the treatment of minor sore throat and minor mouth or throat irritation. Isolation and identification of antimicrobial compound from Mentha longifolia L. leaves grown wild in Iraq. Al-Bayati FA No
19526786 OBJECTIVE: To investigate the chemical constituents of the aerial parts of Polygala sibirica systematically.METHODS: The chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data.RESULT: Ten compounds were isolated and identified as 6-hydroxy-1, 2, 3, 7-tetramethoxy xanthone (1); 1, 2, 3, 6, 7-heptamethoxy xanthone (2); 1, 7-didydroxy-2, 3-methylenedioxy xanthone (3); 1, 7-dihydroxy-2, 3-dimethoxy xanthone (4); 1, 3, 7-trihydroxy-2-methoxy xanthone (5); 1, 6, 7-trihydroxy-2, 3-dimethoxy xanthone (6); alpha-spinasterol (7); alpha-spinasterolic-3-O-beta-D-glucoside (8); squalene (9) and polygital (10).CONCLUSION: Compounds 1-10 were isolated from this species for the first time. [Studies on xanthones from aerial parts of Polygala sibirica]. Song Y, Jiang Y, Zhou S, Bi D, Tu P. No
19526787 OBJECTIVE: To study the chemical constituents of the leaves of Psidium guajava.METHOD: The chemical constituents were isolated by column chromatography on silica gel, Sephadex LH-20 and MPLC. Their structures were elucidated on the basis of spectral analysis.RESULT: Nine compounds were isolated from this plant, and the structure of them were identified as ursolic acid (1), 2alpha-hydroxyursolic acid (2), 2alpha-hydroxyoleanolic acid (3), morin-3-O-alpha-L-arabopyranoside (4), quercetin (5), hyperin (6), myricetin-3-O-beta-D-glucoside (7), quercetin-3-O-beta-D-glucuronopyranoside (8), 1-O-galloyl-beta-D-glucose (9).CONCLUSION: Compounds 3, 7-9 were isolated from this plant for the first time. [Studies on chemical constituents of leaves of Psidium guajava]. Fu H, Luo Y, Zhang D. No
19526788 OBJECTIVE: To isolate and identify the active xanthone glycosides in Halenia elliptica. METHOD: The compounds were isolated by column chromatography, semi-preparative high performance liquid chromatography and related techniques. Their structures were elucidated through spectroscopic analysis (NMR and MS).RESULT: Six xanthone glycosides were isolated and identified as: 2,3,5-trimethoxy-1-O-primeverosyloxyxanthone (1), 2, 3, 4, 5-tetramethoxy-1-O-primeverosyloxyxanthone (2), 2, 3, 5, 7-tetramethoxy-1-O-primeverosyloxyxanthone (3), 2, 3, 7-trimethoxy-1-O-primeverosyloxyxanthone (4), 2, 3, 4, 7-tetramethoxy-1-O primeverosyloxyxanthone (5), and 2, 3, 4, 5, 7-pentamethoxy-1-O-primeverosyloxyxanthone (6).CONCLUSION: Compounds 4-6 were isolated from this plant for the first time. [Xanthone glycosides from Tibetan herb Halenia elliptica]. Liu X, Liu Y, Shi Y. No
19552385 Sixteen new D-ring-opened phragmalin limonoid orthoesters, swietenitins A-M (1-6, 8, 10, 12-16), 2-acetoxyswietenialide D (7), 2,11-diacetoxyswietenialide D (9), and 11-deoxyswietenialide D (11), and four known compounds were isolated from the twigs of Swietenia macrophylla. The structures of 1-16 were established on the basis of spectroscopic methods, and 1 and 2 were confirmed by single-crystal X-ray diffraction. The (1)H NMR-based conformational analysis on the epimeric compounds 1 and 2 provided a general approach to determine the absolute configuration of the 2,3-epoxy-2-methylbutyryloxy unit at C-3 borne by a large group of the known phragmalin-type limonoid orthoesters. D-ring-opened phragmalin-type limonoid orthoesters from the twigs of Swietenia macrophylla. Lin BD, Zhang CR, Yang SP, Zhang S, Wu Y, Yue JM. No
19557364 Two new secoiridoid glycosides, 4'''-O-beta-D-glucopyranosyltrifloroside 1 and 4'''-O-beta-D-glucopyranosylscabraside 2, along with three known secoiridoids were isolated from the rhizomes of Gentiana scabra (Gentianaceae) in our recent phytochemical study. Their chemical structures were determined by spectroscopic data including 1D and 2D NMR spectra. The chemotaxonomic significance of the secoiridoid glycosides is briefly discussed. Two new secoiridoid glycosides from the rhizomes of Gentiana scabra Bunge. Kim JA, Son NS, Son JK, Jahng Y, Chang HW, Jang TS, Na M, Lee SH. No
19563855 We have investigated the chemical composition of Piper gaudichaudianum essential oil, as well as its cytotoxic, mutagenic and genotoxic effects in V79 cells. The chemical analyses showed that the major compounds are (E)-nerolidol (22.4%), alpha-humulene (16.5%), (E)-caryophyllene (8.9%) and bicyclogermacrene (7.4%). Dose-dependent cytotoxic effects were observed in V79 cells treated with essential oil by using clonal survival, 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide reduction assay (MTT) and trypan blue exclusion assay (TB), and a significant decrease in survival was observed at concentrations of 0.5 microg/mL and higher. The P. gaudichaudianum essential oil treatment caused DNA strand breaks in V79 cells at concentrations up to 2 microg/mL, as detected by the alkaline comet assay, but did not induce double-strand breaks, as verified by neutral comet assay. It induced a significant increase in the frequency of micronucleated cells at 4, 6 and 10 microg/mL. Moreover, P. gaudichaudianum essential oil significantly increased lipid peroxidation at doses of 0.5 microg/mL and higher, suggesting that the observed oxidant potential can be responsible, at least in part, for its cytotoxic and genotoxic effects. Chemical composition and cytotoxic, mutagenic and genotoxic activities of the essential oil from Pipergaudichaudianum Kunth leaves. Péres VF, Moura DJ, Sperotto AR, Damasceno FC, Caramão EB, Zini CA, Saffi J. No
19565711 OBJECTIVE: To study the chemical constituents of Chelonopsis albiflora.METHODS: The compounds were isolated and purified by various column chromatographic techniques and their structures were elucidated on the basis of spectral analysis (EI-MS), 1H-NMR, 13C-NMR.RESULTS: Seven compounds were isolated and identified as 1beta-hydroxylupenol (1), lupenol (2), beta-sitosterol (3), 13-epi-torulosal (4), 19-acetoxy-13-hydroxylabada-8 (17), 14-diene (5), (E)-p-methyl cinnamic acid (6) and vanillic acid (7) .CONCLUSION: All compounds are isolated for the first time from this genus. [Studies on the chemical constituents of Chelonopsis albiflora]. Chen XH, Peng C. No
19571394 Rhinacanthone, a main bioactive naphthoquinone, isolated from roots of Rhinacanthus nasutus KURZ, (family Acanthaceae), a Thai traditional medicine, has been reported to possess anticancer effects, although the anticancer mechanism is still unclear. Therefore, we investigated the effects of rhinacanthone on cell proliferation, cell cycle progression and apoptosis induction in human cervical carcinoma (HeLa) cells. beta-Lapachone, an anticancer drug having a chemical structure related to rhinacanthone, was used as a positive control. The results demonstrated that rhinacanthone inhibited proliferation of HeLa cells in a dose-dependent manner and had greater efficacy than that of beta-lapachone: IC(50) values of the compound ranged from 1.2+/-0.1 to 5.5+/-0.86 muM for 2-24 h time periods. Rhinacanthone-treated HeLa cells displayed several apoptotic features as evidenced by the appearance of chromatin condensation, internucleosomal DNA fragmentation, increase in the proportion of sub G(1) apoptotic cells, and externalization of annexin-V. The apoptotic processes by the treatment with rhinacanthone involved in a marked increase in the level of pro-apoptotic protein Bax and decrease in the levels of anti-apoptotic proteins Bcl-2 and survivin as well as subsequent activation of caspase-9 and caspase-3. Moreover, rhinacanthone increased the expression of apoptosis-inducing factor (AIF) which would translocate from mitochondria to nucleus through cytosol, and induce apoptosis through caspase independent signaling pathway. Taken together, our findings for the first time demonstrate that rhinacanthone-induced apoptosis in HeLa cells is mediated primarily through the mitochondria-dependent signaling pathway, suggesting that it may be a promising agent for the treatment of human cervical cancer. Induction of apoptosis by rhinacanthone isolated from Rhinacanthus nasutus roots in human cervical carcinoma cells. Siripong P, Hahnvajanawong C, Yahuafai J, Piyaviriyakul S, Kanokmedhakul K, Kongkathip N, Ruchirawat S, Oku N. No
19571423 Phytochemical investigation of the leaves of Tamarix nilotica (Tamaricaceae) has led to isolation of methyl ferulate 3-O-sulphate (1) for the first time from natural sources. In addition, coniferyl alcohol 4-O-sulphate (2), kaempferol 4'-methyl ether (3), tamarixetin (4) and quercetin 3-O-beta-D-glucupyranuronide (5) were isolated from the n-butanol soluble fraction of the extract. The pentacyclic triterpenoid, 3alpha-(3'',4''-dihydroxy-trans-cinnamoyloxy)-D-friedoolean-14-en-28-oic acid (6) was isolated from the n-hexane soluble fraction of the extract. The structures of these compounds were determined on the basis of spectroscopic analyses including 2 dimensional NMR. Compounds 3, 4 and 6 exhibited 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity with IC(50) values of 35.2, 37.0 and 21.2 muM, respectively. A new ferulic acid ester and other constituents from Tamarix nilotica leaves. Abouzid SF, Ali SA, Choudhary MI. No
19631313 Phytochemical analysis of Solanum nigrum has resulted in the isolation of two novel disaccharides. Their structures were determined as ethyl beta-D-thevetopyranosyl-(1-->4)-beta-D-oleandropyranoside (1) and ethyl beta-d-thevetopyranosyl-(1-->4)-alpha-D-oleandropyranoside (2), respectively, by chemical and spectroscopic methods. Two novel oligosaccharides from Solanum nigrum. Chen R, Feng L, Li HD, Zhang H, Yang F. No
19634338 The hydrodistilled essential oils from flower, leaf and stem of Chaerophyllum macropodum Boiss. were analyzed by GC and GC/MS. The flower oil was characterized by high amounts of myristicin (42.5%) and trans-beta-ocimene (41.0%). In the leaf oil eighteen constituents, representing 99.3% of the total, were identified of which trans-beta-ocimene (24.9%), myristicin (15.7%), terpinolene (14.5%), fenchyl acetate (13.9%), cis-beta-ocimene (6.3%) and sabinene (6.1%) were major components. The main components of the stem oil were trans-beta-ocimene (54.2%), myristicin (22.4%) and sabinene (8.9%). Antibacterial activities of the flower, leaf and stem oils were evaluated using the micro-dilution broth method. Inhibitory effects on Escherichia coli and Salmonella typhi were recorded. Antibacterial activity and composition of essential oils from flower, leaf and stem of Chaerophyllum macropodum Boiss. from Iran. Shafaghat A. No
19634482 The aim of the present study was the evaluation of the antibacterial activity of Fennel essential oil on isolates of Acinetobacter baumannii. Forty eight isolates were collected from clinical specimens from burn wards of hospitals in Tehran, Iran between April and September, 2006. The susceptibility of isolates was determined using a broth microdilution method. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of isolates to Fennel essential oil were determined. The susceptibilities of isolates to different antibiotics were tested using agar disk diffusion method. The rates of resistance were determined to antibiotics as follows: cefazolin 100%, ciprofloxacin 100%, ofloxacin 95.8%, kanamycin 95.8%, carbenicillin 93.7%, ticarcillin 93.7%, piperacillin 88.9%, co-trimoxazole 79.1%, ceftizoxime 75%, gentamicin 70.8%, cefalotin 60.4%, amikacin 52% and imipenem 14.6%. Fennel essential oil possessed antibacterial effect against all isolates of A. baumannii. These results suggest the potential use of the Fennel essential oil for the control of multi-drug resistant A. baumannii infections. However, more adequate studies must be carried out to verify the possibility of using it for fighting bacterial infections in human. Antibacterial effects of Iranian fennel essential oil on isolates of Acinetobacter baumannii. Jazani NH, Zartoshti M, Babazadeh H, Ali-daiee N, Zarrin S, Hosseini S. No
19635670 Four natural pyranocoumarins clausenidin (1), nordentatin (2), clausarin (3), and xanthoxyletin (4) were isolated from the medicinal plant Clausena excavata. Recently, we found that 1 and 2 suppressed hepatitis B virus surface antigen in HepA2 cells, and in addition, 1-3 showed cytotoxic activity against four human cancer cell lines (A549, MCF7, KB, and KB-VIN). To explore the SAR of 1-4, 17 pyranocoumarin analogues (5-21) were designed and synthesized. Among these analogues, 5 and 10 were the most potent against hepatitis B virus with EC(50) values of 1.14 and 1.34microM, respectively. The most interesting result in the cytotoxicity assay was the significant activity of 1, 5, and 6 against the multi-drug resistant cell line, KB-VIN, without activity against the KB cell line. These data suggest that these three compounds could be useful hits for developing MDR-inverse drugs. Anti-HBV and cytotoxic activities of pyranocoumarin derivatives. Su CR, Yeh SF, Liu CM, Damu AG, Kuo TH, Chiang PC, Bastow KF, Lee KH, Wu TS. No
19639786 OBJECTIVE: To study the chemical constituents from the antioxidant fraction of Neo-Taraxacum siphonathum.METHOD: Various chromatographic techniques were used to isolate and purify the constituents. The structures were elucidated on the basis of chemical evidence and spectral analysis.RESULT: Ten compounds were isolated and identified from Neo-T. siphonathum, caffeic acid (1), chlorogenic acid (2), quercetin (3), luteolin (4), quercetin-3-O-beta-D-glucopyranoside (5), quercetin-3-O-alpha-D-arabinofuranoside (6), quercetin-3-O-alpha-D-arabinopyranoside (7), luteolin-7-O-beta-D-glucopyranoside (8), beta-sitosterol (9) and daucosterol (10).CONCLUSION: Compounds 1-10 were isolated from this plant for the first time. [Chemical constituents from Neo-Taraxacum siphonathum]. Shi S, Zhou H, Zhang Y, Huang K, Liu S. No
19652415 New quassinoids, delaumonones A (1) and B (2) have been isolated from the bark of Laumoniera bruceadelpha NOOTEBOOM (Simaroubaceae) and the structures were elucidated by 2D NMR analysis and a chemical correlation. Delaumonones showed an antimalarial activity against Plasmodium falciparum. Delaumonones A and B, new antiplasmodial quassinoids from Laumoniera bruceadelpha. Oshimi S1, Takasaki A, Hirasawa Y, Hosoya T, Awang K, Hadi AH, Ekasari W, Widyawaruyanti A, Morita H. No
19666102 Abstarct:"AIM OF THE STUDY:Egyptian medicinal plants are well known by their diverse uses in traditional folk medicine to cure various ailments including infectious diseases. Forty-two Egyptian medicinal plant species were selected from local market and were subjected to antiviral screening bioassay to investigate and to evaluate their biological activities.MATERIALS AND METHODS:Hydro-alcoholic extracts of each species were separately prepared and tested against three viruses: herpes simplex-1 virus (HSV), poliomyelitis-1 virus (POLIO) and vesicular stomatitis virus (VSV). The antiviral activity were determined by means of the end point titration technique (EPTT) that depends on the ability of plant extract dilutions to inhibit the produced cytopathogenic effect (CPE) and expressed as reduction factor (Rf) of the viral titer.RESULTS:Achillea fragrantissima, Jasonia montana and Globularia arabica are found to have antiviral activity against POLIO in a concentration dependent manner at complete non-toxic concentration range 10-100 microg/ml (Rf 10(6)), 10-100 microg/ml (Rf 10(5)) and 50-100 microg/ml (Rf 10(4)), respectively while Tanacetum sinaicum are found to have moderate antiviral activity against POLIO at concentration of 50-100 microg/ml (Rf 10(2)). Ephedra alata and Moringa peregrina are found to have antiviral activity against HSV (Rf 10(4)). Also, the results revealed that Capparis sinaica, Tamarix nilotica and Cyperus rotundus are found to have virucidal effect against HSV. All the forty-two plant species are found to have no reliable antiviral activity against VSV." Antiviral screening of forty-two Egyptian medicinal plants. Soltan MM, Zaki AK. No
19673381 OBJECTIVE: To study the chemical constituents in roots of Saussurea lappa.METHOD: Isolation and purification were carried out by silica gel, Sephadex LH-20 and RP-18 column chromatography. The chemical structures of constituents were elucidated on the basis of spectral data.RESULT: Eleven compounds were isolated and identified as: 5,7-dihydroxy-2-methylchromone (1), p-hydroxybenzaldehyde (2), 3,5-dimethoxy-4-hydroxy-benzaldehyde (3), 3,5-dimethoxy-4-hydroxy-acetophenone (4), ethyl 2-pyrrolidinone-5(s)-carboxylate (5), 5-hydroxymethyl-furaldehyde (6), palmitic acid (7), succinic acid (8), glucose (9), daucosterol (10), beta-sitosterol (11).CONCLUSION: Compounds 1, 2, 4, 5, 7, 9 were isolated from the genus Saussurea for the first time. [Study on chemical constituents from roots of Saussurea lappa]. Zhang T, Wang H, Du G, Chen R. No
19695651 An ellagitannin monomer, nilotinin M1 (1), and three dimers, nilotinins D1 (2), D2 (3), and D3 (4), were isolated from leaves of Tamarix nilotica (Ehrenb.) Bunge. Structures were elucidated based on analysis of spectroscopic data and chemical correlations with known compounds. In addition, six known tannins, hirtellin A (5) (dimer), remurin A (6), remurin B (7), 1,3-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-beta-D-glucose (8), gemin D (9), and hippomanin A (10) (monomers), were isolated for the first time from this plant species. The reported (13)C NMR assignments of the dehydrodigalloyl moiety and glucose cores of 5 are revised, and the (13)C NMR spectroscopic data for 6 and 7 are also reported for the first time. Monomeric and dimeric hydrolysable tannins of Tamarix nilotica. Orabi MA, Taniguchi S, Hatano T. No
19705860 Five new isoflavones, named 5,4'-dihydroxy-7-methoxy-3'-(3-methylbuten-2-yl)isoflavone (1), 5,2',4'-trihydroxy-7-methoxy-5'-(3-methylbuten-2-yl)isoflavone (2), 5,4'-dihydroxy-7-methoxy-3'-(3-methyl-2-hydroxybuten-3-yl)isoflavone (3), 3'-formyl-5,4'-dihydroxy-7-methoxyisoflavone (4), and 5-hydroxy-3''-hydroxy-2'',2''-dimethyldihydropyrano[5'',6'':3',4']isoflavone (5), as well as six known compounds, wighteone (6), 3'-isoprenylgenistein (7), isolupabigenin (8), alpinumisoflavone (9), erypoegin D (10), and crystacarpin (11), were isolated from Erythrina poeppigiana. The structures of the isolated compounds were elucidated on the basis of chemical and spectroscopic analysis. The affinity of these compounds for the estrogen receptors ERalpha and ERbeta was evaluated using a receptor binding assay. While isoprenyl and dimethylpyrano substituents in ring A reduced the affinity of binding to ERbeta ca. 100-fold compared to genistein, the isoprenyl substituent in ring B was better accommodated, allowing 7 to bind with ca. 10-fold lower affinity than genistein. Isoflavonoids from Erythrina poeppigiana: evaluation of their binding affinity for the estrogen receptor. Djiogue S1, Halabalaki M, Alexi X, Njamen D, Fomum ZT, Alexis MN, Skaltsounis AL. No
19708004 Two new steroidal glycosides, named dioseptemlosides I (1) and J (2), along with two known trihydroxy fatty acids, (12 Z,15 Z)-9,10,11-trihydroxy-12,15-octadecadienoic acid (3) and (12 Z)-9,10,11-trihydroxy-12-octadecenoic acid (4), were isolated from the rhizomes of Dioscorea septemloba. Their structures were determined by HRESIMS, 1D and 2D NMR experiments, physical data, and chemical methods. The antitumor activity of compounds 1-4 was evaluated against three tumor cell lines and all of them were inactive at a concentration of 10 microM. Cholestane glycosides and trihydroxy fatty acids from the rhizomes of Dioscorea septemloba. Liu XT1, Wang ZZ, Xiao W, Zhao HW, Yu B. No
19721265 Two new triterpenoid saponins, brachyposide A (1) {3-O-beta-D-galactopyranosyl-(1-->2)-[beta-D-xylopyranosyl-(1-->3)]-beta-D-glucuronopyranosyl-2beta,3beta,16alpha,23-tetrahydroxyolean-12-en-28-oic acid 28-O-beta-D-apiofuranosyl-(1-->3)-beta-D-xylopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranosyl ester} and brachyposide B (2) {3-O-beta-D-glucopyranosyl-(1-->3)-beta-D-galactopyranosyl-(1-->3)-beta-D-glucuronopyranosyl-2beta,3beta,23-trihydroxyolean-12-en-28-oic acid 28-O-beta-D-xylopyranosyl-(1-->4)-[beta-D-apiofuranosyl-(1-->3)]-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranosyl ester}, together with four known triterpenoid saponins, including tabguticoside A, nipponoside D, palmatoside E and ciwujianoside A(1), were isolated from the stem bark of Acanthopanax brachypus. Their structures were elucidated on the basis of spectroscopic and chemical evidence. Triterpenoid saponins from the stem bark of Acanthopanax brachypus HARMS. Hu HB1, Zheng XD, Cao H, Guo XQ, Hu HS. No
19731609 The essential oil of the aerial parts of Chenopodium ambrosioides L. has been isolated by hydrodistillation and analyzed using GC-MS. The major components were found to be alpha-terpinene (63.1%), p-cymene (26.4%) and ascaridole (3.9%). The oil displayed no antibacterial activity against either Gram-positive bacteria Bacillus cereus or Staphylococcus aureus, or the Gram-negative bacterium Escherichia coli (MIC=1250 microg/mL). A cluster analysis of C. ambrosioides essential oils reveals at least seven distinct chemotypes: ascaridole, alpha-terpinene, alpha-pinene, p-cymene, carvacrol, alpha-terpinyl acetate, and limonene. Volatile constituents and antibacterial screening of the essential oil of Chenopodium ambrosioides L. growing in Nigeria. Owolabi MS, Lajide L, Oladimeji MO, Setzer WN, Palazzo MC, Olowu RA, Ogundajo A. No
19739650 Four new alkaloids, 17-hydroxydaphnigraciline (1), subdaphnidine A (2), daphnezomine L methyl ester (3), and 11-hydroxycodaphniphylline (4), along with 24 known analogues, were isolated from the leaves of Daphniphyllum subverticillatum. The structures of 1-4 were elucidated on the basis of spectroscopic methods and from chemical evidence. Daphnilongeridine (5) showed cytotoxicity against several tumor cell lines at IC(50) values in the range 2.4-9.7 microM and against the HMEC human microvascular endothelial cell line with an IC(50) of 2.7 microM. Alkaloids from the leaves of Daphniphyllum subverticillatum. Zhang CR1, Liu HB, Feng T, Zhu JY, Geng MY, Yue JM. No
19746844 We report on the antiviral potency of an aqueous extract of root/stem bark of Rhus aromatica (fragrant sumac extract) against herpes simplex virus type 1 and type 2 in cell culture (RC-37 cells) using a plaque reduction assay. The extract exhibited a high level of anti-HSV activity with IC50-values of 0.0005% for HSV-1 and 0.0043% for HSV-2 as well as high selectivity indices (SI) of 5400 for HSV-1 and 628 for HSV-2. In order to determine the mode of antiviral action, the fragrant sumac extract was added at different times to the cells or viruses during the viral infection cycle. At maximum non-cytotoxic concentration (0.25%), plaque formation was significantly reduced by more than 99% when herpes simplex viruses were pretreated with the plant extract for 1 h prior to cell infection. When the host cells were pretreated with the fragrant sumac extract for 1 h prior to virus infection, the infectivity of viruses was reduced by 50% for HSV-1 but only moderately for HSV-2. No antiviral effect was seen when the plant extract was added to already infected host cells. Based on these findings the plant extract seems to interact not only with the viral envelope but also with the surface of the host cells impairing the ability of herpes simplex viruses to adsorb to and penetrate into the host cells. In conclusion, the aqueous fragrant sumac extract revealed a strong antiviral activity against HSV-1 and HSV-2 in vitro. Antiviral activity of Rhus aromatica (fragrant sumac) extract against two types of herpes simplex viruses in cell culture. Reichling J, Neuner A, Sharaf M, Harkenthal M, Schnitzler P. No
19747699 Two amides, heitziamide A and heitziamide B and two phenylethanoids, heitziethanoid A and heitziethanoid B together with thirteen known compounds were isolated from F. heitzii (Letouzey). The structures of all compounds were established by spectroscopic analysis. Nine compounds were evaluated for oxidative burst inhibitory activity in a chemoluminescence assay and for cytotoxicity against PC-3 prostate cancer cells. All compounds exhibited a clear suppressive effect on phagocytosis response upon activation with serum opsonized zymosan at the range of IC(50)=2.0-6.5 microM, but no cytotoxic effect was observed (IC(50)>100 microM). Oxidative burst inhibitory and cytotoxic amides and lignans from the stem bark of Fagara heitzii (Rutaceae). Mbaze LM1, Lado JA, Wansi JD, Shiao TC, Chiozem DD, Mesaik MA, Choudhary MI, Lacaille-Dubois MA, Wandji J, Roy R, Sewald N. No
19766276 A xanthonolignoid, 2-O-demethylkielcorin, and a phenylxanthone, chinexanthone A, were isolated from stems of Hypericum chinense together with four known xanthonolignoids and seven known xanthones. Their structures were established by spectroscopic analysis, as their optical properties and absolute stereochemistry determined. The cytotoxicities of the isolated xanthone derivatives as well as additional 32 xanthones against a panel of human cancer cell lines were also evaluated. Xanthones from Hypericum chinense and their cytotoxicity evaluation. Tanaka N, Kashiwada Y, Kim SY, Sekiya M, Ikeshiro Y, Takaishi Y. No
19771841 OBJECTIVE: To study the chemical constituents of Pithecellobium clypearia.METHODS: The compounds were isolated from Pithecellobium clypearia with column chromatography. Their structures were characterized on the basis of their physical and chemical preperties, as well as chromatographic and spectroscopic evidences.RESULTS: A pair of isomeric flavans consisting of 7, 3'-O-di-gallyoltricetiflavan (I) and 7,4'-O-di-gallyoltricetiflavan (II), along with a catechin compound (-)-epigallocatechin-7-O-gallate (III) were isolated from the leaves and twigs of Pithecellobium clypearia.CONCLUSION: Compound I is a new flavan. Compound III is isolated from Pithecellobium genus for the first time, and its 13C-NMR data is firstly given in the present study. [Studies on the chemical comstituents of Pithecellobium clypearia]. Su MX, Tang ZY, Huang WH, Li YL, Cen YZ. No
19771843 OBJECTIVE: To study the chemical constituents of the root and rhizoma of Ligusticum jeholense.METHODS: The constituents were isolated by silica gel column chromatography, Sephadex LH-20 column chromatography and their structures were elucidated through spectral analysis.RESULTS: Seven compounds were separated from the EtOH extracts. Their structures were identified as levistolide A (1), xiongterpene (2), linoleic acid (3), sucrose (4), daucosterol (5), ferulic acid (6) and beta-sitosterol (7).CONCLUSION: Compounds 1-5 are isolated from the genus for the first time. [Studies on the chemical constituents of the root and rhizoma of Ligusticum jeholense]. Zhang B, Sun JM, Chang RL, Zhang H. No
19778089 Five new (+)-oleuropeic acid derivatives, eucalmaidins A-E (1-5), together with 12 known compounds (6-17), were isolated from the fresh leaves of Eucalyptus maideni. Structures of the new compounds were determined on the basis of spectroscopic analyses (HSQC, HMBC, and (1)H-(1)H COSY), chemical degradation, and enzymatic hydrolysis. Of the tested compounds, only quercetin showed slight anti-herpes simplex virus 1 (HSV-1) activity in vitro. Eucalmaidins A-E, (+)-oleuropeic acid derivatives from the fresh leaves of Eucalyptus maideni. Tian LW, Zhang YJ, Wang YF, Lai CC, Yang CR. No
19795885 Ten new triterpenoid saponins (1-10), named rubusides A-J, and 21 known saponins (11-31) were isolated from the roots of Rubus ellipticus var. obcordatus. The structures of 1-10 were established on the basis of spectroscopic analyses, mainly NMR and MS, and chemical degradations. The compounds demonstrated inhibitory activities against alpha-glucosidase with IC50 values in the range 0.65-3.09 mM. Triterpenoid saponins from Rubus ellipticus var. obcordatus. Li W, Fu H, Bai H, Sasaki T, Kato H, Koike K. No
19799675 The growth-inhibiting activities of Sinapis alba L. seed-derived materials were examined on the growth of Bifidobacterium bifidum, B. breve, B. longum, Clostridium difficile, C. perfringens, Escherichia coli, Lactobacillus acidophilus, and L. casei. The active component of S. alba seeds was purified using silica gel column chromatography and HPLC and was identified as phenethyl isothiocyanate by various spectroscopic analyses. The antimicrobial activity of phenethyl isothiocyanate varied according to the dose and bacterial strain tested. Phenethyl isothiocyanate strongly inhibited the growth of C. difficile and C. perfringens at 1 mg/disc, and weakly (+) inhibited its growth at 0.1 mg/disc. Furthermore, phenethyl isothiocyanate moderately (++) inhibited the growth of E. coli at a dose of 2 mg/disc, but did not inhibit the growth of bifidobacteria and lactobacilli. Addition of various functional groups to isothiocyanates resulted in selective inhibitory activity against harmful bacteria with low concentrations of aromatic isothiocyanates demonstrating greater inhibitory activity against clostridia and E. coli than aliphatic isothiocyanates. In conclusion, aromatic isothiocyanates containing phenethyl-, benzyl-, and benzoyl-groups might be useful in the development of novel preventive and therapeutic agents against diseases caused by harmful intestinal bacteria. Growth-inhibiting activities of phenethyl isothiocyanate and its derivatives against intestinal bacteria. Kim MG, Lee HS. No
19809919 Ten compounds were isolated from the fruits of Hippophae rhamnoides. On the basis of spectroscopic and chemical methods, the structures of these compounds were elucidated as hippophae cerebroside (1), oleanolic acid (2), ursolic acid (3), 19-alpha-hydroxyursolic acid (4), dulcioic acid (5), 5-hydroxymethyl-2-furancarbox-aldehyde (6), cirsiumaldehyde (7), octacosanoic acid (8), palmitic acid (9) and 1-O-hexadecanolenin (10). Among them, 1 was a new compound, and 4-7 and 10 were obtained from the genus for the first time. Chemical constituents from the fruits of Hippophae rhamnoides. Zheng RX, Xu XD, Tian Z, Yang JS. No
19873730 Abstarct:"OBJECTIVE:To study the chemical constituents of the fruit handles from Schizandra chinensis.METHODS:Compounds from the 85% ethanol extracts were isolated by silica gel, Sephadex LH-20, recrystal, etc., and their structures were identified by the spectral analysis and chemical evidence.RESULTS:Eight compounds were isolated and identified as wuweizisu C (I), ganwuweizic acid(II), beta-sitosterol(III), gomisin A(IV), schizandrin(V), daucosterol(VI), wuweizisu A(VII), gamma-schizandrin (VIII).CONCLUSION:Compounds I - VIII are isolated from the fruit handles of Schizandra chinensis for the first time." [Study on the chemical constituents of the fruit handles from Schizandra chinensis]. Shi L, He XX, Pan Y, Han L, Yang XO, Zhao YQ. No
19873731 OBJECTIVE: To study the chemical constituents of Uvaria microcarpa.METHODS: The constituents were repeatedly separated and purified with silica gel column and Sephadex LH-20 column, and identified by physico-chemical properties and spectral methods.RESULTS: Nine compounds were separated and identified as beta-sitosterol palmitate (I), euphorginol (II), beta-sitosterol (III), benzoic acid(IV), stigmasterol-3-O-beta-D-glucopyranoside (V), taraxerol (VI), emodin (VII), aristololactam A II (VI), beta-daucosterol (IX) .CONCLUSION: Compounds I, II, V, V are isolated from this plant for the first time. [Study on the chemical constituents of Uvaria microcarpa]. Lu RM, Su X, Zhou YY, Wei JH. No
19873778 OBJECTIVE: To study the chemical constituents of the roots of Incarvillea mairei.METHOD: The chemical constituents were isolated and purified by chromatographic techniques with silica gel, Sephadex LH-20 column, and preparative TLC. Structures of the compounds were identified by spectroscopic methods.RESULT: Seven compounds were obtained and elucidated as 1-O-methyl-guaiacylglycerol (1), 1-O-feruloyl-3-O-(26"-hydroxylhexacosoyl) glycerol (2), incarvine D (3), piceid (4), 6'-8"E, 11"E-octadecadienoyl-clionasterol-3-glucoside (5), beta-sitosterol (6), and beta-daucosterol (7).CONCLUSION: Compounds 1-7 were isolated from I. mairei, and among them 1 and 2 were new compounds, 4, 5 were isolated from the genus Incarvillea for the first time. [Chemical constituents from roots of Incarvillea mairei]. Huang Z, Zhang W, Lin S, Liu C, Huang D, Song T, Lu L, Pei Y. No
19873783 OBJECTIVE: To investigate the chemical constituents in herbs of Polygonum jucundum.METHOD: The 85% ethanol extract was separated by means of silica gel and Sephadex LH-20 column chromatography. The compounds isolated from the plant were identified by physicochemical properties and spectroscopic evidence.RESULT: Eight compounds were isolated and identified as: quercetin-3'-O-beta-D-galactoside (1), 8-methoxyquercetin (2), pigenin (3), luteolin (4), quercetin (5), 3,5,7-trihydroxychromone (6), p-hydroxybenzaldehyde (7), beta-sitosterol (8).CONCLUSION: All compounds were isolated from this plant for the first time, compounds 5- 8 were isolated from the genus Polygonum for the first time. [Chemical constituents in herbs of Polygonum jucundum]. Lin Y, Zhang C, Zhang M. No
19881272 Chemical investigation on the whole plant of Houttuynia cordata has resulted in the isolation of two new compounds, named as houttuynoside A (1) and houttuynamide A (2), together with thirty-eight known compounds. The structures of 1 and 2 were elucidated on the basis of spectroscopic analysis. In the inhibitory effects on herpes simplex virus type 1 (HSV-1) assay, norcepharadione B (10) showed good inhibitory activity against the replication of HSV-1. In addition, the antioxidant and antityrosinase activities of some isolated compounds were also evaluated. Among these compounds, quercitrin (25) and quercetin-3-O-beta-D-galactopyranoside (26) showed excellent 2,2-diphenyl-1-picrylhydrazyl radical-scavenging property with IC50 values of 31 and 63 microM, respectively. Cepharadione B (9) exhibited strong tyrosinase inhibitory activity with an IC50 value of 170 microM. The constituents and their bioactivities of Houttuynia cordata. Chou SC, Su CR, Ku YC, Wu TS. No
19894511 OBJECTIVE: To study the compounds from the whole plant of Galium aparine var. tenerum.METHOD: The various column chromatographic techniques were applied to isolate the chemical constituents. MS, NMR and 2D-NMR spectroscopic techniques were uesd to identify chemical structures.RESULT: Six compounds were isolated from the EtOAc-soluble part of the 95% ethanol extract of the plant, and their chemical structures were identified as 1-(4-hydroxyphenyl)-ethanone (1), vanillic acid (2), 3,4-dihydroxybenzoic acid (3), p-hydroxycinnamic acid (4), gallic acid (5), 4-hydroxytruxillic acid (6).CONCLUSION: The NMR data of compound 6 were completely assigned by 2D-NMR techniques, including 1H-1H COSY, HMQC and HMBC spectra. This was the first time to report isolation of the compound 6 from natural product. The compounds 1-5 was isolated from the genus Galium for the first time. [Phenolic compounds from Galium aparine var. tenerum]. Yang J, Cai X, Mu S, Yang X. No
19921259 In order to prevent the destruction of the ecology and to sustain the flora mainly for medicinal plants, we investigated on alternative parts taken from four plants already known to display antiplasmodial activities and largely used by traditional healers in sub-Saharan Africa. The evaluated parts are bark of trunk for Zanthoxylum zanthoxyloides and leaves for Sarcocephalus latifolius instead of roots, and leaves for Combretum molle and Anogeissus leiocarpus instead of stem bark. The antiplasmodial activity of extracts of these plants was evaluated in vitro using the multi-resistant strain (W2) of Plasmodium falciparum. Antiproliferative activity was also assessed, using K562S human monocyte cell lines, along with calculation of the selectivity index (SI) of each extract. The highest in vitro antiplasmodial activity was found in the alkaloid extract of trunk bark from Z. zanthoxyloides and from the MeOH extract of A. leiocarpus leaves (IC(50) = 1.2 microg/mL and 4.9 microg/mL, respectively) with good selectivity index. Moderate activity was found in the MeOH extract (IC(50) = 5.7 microg/mL) and MeOH/H2O extract (IC(50) = 7.9 microg/mL) of C. molle leaves. Moderate activity was also found in the MeOH/H20 extract (IC(50) = 5.2 microg/mL) and the decoction (IC(50) = 8.2 microg/mL) from leaves of A. leiocarpus. No good activity was found with extracts from roots of S. latifolius. All extracts tested displayed low levels of cytotoxicity against K562S cells. The data generated clearly show that the trunk bark for Z. zanthoxyloides and the leaves for A. leiocarpus and C. molle could be used for the treatment of malaria instead of roots and stem bark. Antiplasmodial activity and toxicity of crude extracts from alternatives parts of plants widely used for the treatment of malaria in Burkina Faso: contribution for their preservation. Gansané A, Sanon S, Ouattara LP, Traoré A, Hutter S, Ollivier E, Azas N, Traore AS, Guissou IP, Sirima SB, Nebié I. No
19924033 One new drimane-type sesquiterpenoid, named ugandenial A (1), was isolated from the ethyl acetate extract of the bark of Warburgia ugandensis (Canellaceae) together with eight known drimane-type sesquiterpenoids: 11alpha-hydroxycinnamosmolide (2), warburganal (3), polygodial (4), mukaadial (5), dendocarbin A (6), 9alpha-hydroxycinnamolide (7), dendocarbin L (8) and dendocarbin M (9). Their structures were established by detailed spectroscopic analysis. In addition a keto-enol equilibrium was demonstrated for compound 1 through a detailed NMR analysis run in CD(2)Cl(2) at 190 K. Cytotoxicity of the isolated compounds against KB cells was evaluated. Ugandenial A, a new drimane-type sesquiterpenoid from Warburgia ugandensis. Xu M1, Litaudon M, Krief S, Martin MT, Kasenene J, Kiremire B, Dumontet V, Guéritte F. No
19926279 Six dammarane-type saponins, including three new compounds, floralginsenosides Ta-Tc (1-3), and three known, floralginsenoside Td (4), ginsenoside F(1) (5), and ginsenoside F(5) (6), were isolated from the flower buds of Panax ginseng. Floralginsenoside Td (4) was first isolated from natural plant sources. Their structures were elucidated on the basis of extensive chemical and spectroscopic methods. Compounds 1, 5, and 6 showed cytotoxic activities towards the HL-60 human leukemia cell line with respective IC(50) values of 36.3, 23.2, and 62.4microM. In addition, after the HL-60 cells were treated with these compounds, several apoptosis events, including chromatin condensation and increase in the population of sub-G1 hypodiploid cells, were observed. Dammarane-type saponins from the flower buds of Panax ginseng and their effects on human leukemia cells. Nguyen HT1, Song GY, Kim JA, Hyun JH, Kang HK, Kim YH. No
19937549 The new compounds guttiferone O ( 1) and 3-methoxycheffouxanthone ( 2) have been isolated from the seeds of Garcinia afzelii Engl., together with nine known compounds: 2-hydroxy-1,7-dimethoxyxanthone ( 3), smeathxanthone A ( 4), 1,5-dihydroxyxanthone ( 5), 1,6-dihydroxy-5-methoxyxanthone ( 6), cheffouxanthone ( 7), 1,3,5-trihydroxyxanthone ( 8), smeathxanthone B ( 9), isoxanthochymol ( 10) and guttiferone E ( 11). Their structures were elucidated by means of 1D and 2D-NMR techniques. All the isolates showed high cytotoxic activity and were found inactive when tested against HIV and influenza viruses. Cytotoxicity of natural compounds isolated from the seeds of Garcinia afzelii. Lannang AM, Louh GN, Biloa BM, Komguem J, Mbazoa CD, Sondengam BL, Naesens L, Pannecouque C, De Clercq E, Sayed El Ashry el H. No
19957430 A detailed chemical study of the aerial parts of Tamarix nilotica (Tamaricaceae) from Saudi Arabia led to the isolation of a new pentacyclic triterpenoid, 3-O-trans-caffeoylisomyricadiol, in addition to nine known compounds. The structures of all isolated compounds were unambiguously elucidated by 1D, 2D NMR, and mass spectrometry. In the radical scavenging (DPPH) assay, 3-O-trans-caffeoylisomyricadiol exhibited potent antioxidant activity with an IC50 value of 3.56 microM, while that for quercetin (standard antioxidant) was 5.72 microM. 3-O-trans-caffeoylisomyricadiol: a new triterpenoid from Tamarix nilotica growing in Saudi Arabia. Orfali RS, Ebada SS, El-Shafae AM, Al-Taweel AM, Lin WH, Wray V, Proksch P. No
19960942 OBJECTIVE: To study the volatile oil of Murraya exotica.METHODS: The volatile oil of Murraya exotica was extracted by steam-stilling and was identified by GC-MS-DS.RESULTS: More than 90 compounds were separated, and 59 compounds were identified, accounting for 93.9% of the total essential oil of Murraya exotica. The major constituents of volatile oil were bicyclogermacrene (26.0%), beta-caryophyllene (20.8%), alpha-caryophyllene (5.8%), delta-cadinene (4.7%), spathulenol (4.3%), trans-alpha-bergamotene (4.1%), germacrene D (3.7%), beta-bisabolene (3.0%), ar-Curcumene (2.5%).CONCLUSION: The major components of Murraya exotica are the terpenoids, including 80.6% of sesquiterpenoids and 11.9% of monoterpenoids. Bicyclogermacrene is identified in Murraya genus for the first time. [Study on the volatile oil of Murraya exotica]. Jiang PC, Zhou J, Cao B, Wei GN, He KJ, Liu BM. No
19960943 OBJECTIVE: To study the chemical constituents of the flower of Paulownia fortunei.METHODS: The constituents were isolated by column chromatography and their structures were elucidated through spectroscopic analysis.RESULTS: The compounds were identified as: apigenin (I), luteolin (II), Hesperetin (III), Naringenin-7-O-beta-D-glucoside (IV), Arbutin (V), 4-hydroxybenzyl-beta-D-glucoside (VI), abscisic acid (VII) and 1-acetoxy-3-hydroxypropan-2-yl-3-hydroxypentanoate (VIII).CONCLUSION: All these compounds are obtained from the flower of this plant for the first time. [Studies on the chemical constituents from flower of Paulownia fortunei]. Li XQ, Zhang PF, Duan WD, Zhang DL, Li C. No
19960945 OBJECTIVE: To study the chemical constituents of Dryopteris fragrans.METHODS: The constituents of CHCl3-soluble portion and ethyl acetate-soluble portion from the alcohol extract were isolated and purified by means of chromatography. All the compounds were identified by their physical characteristics and spectral features.RESULTS: Five compounds were isolated and identified as beta-sitosterol (I), rutin (II), quercetin (III), quercetin-3-O-beta-D-pyranglucoside (IV) and 5,7-dihydroxy-2-hydroxymethyl chromone (V).CONCLUSION: Compounds II - V are isolated from this plant for the first time. [Studies on the chemical constituents of Dryopteris fragrans]. Li B, Zhu JF, Zou ZJ, Yin YQ, Shen ZB. No
20015638 The essential oil from fresh leaves of Plinia cerrocampanensis Barrie (Myrtaceae), obtained by hydrodistillation, was analysed by GC-FID and GC-MS. Forty components, representing more than 91% of the oil, were identified. Oxygenated sesquiterpenes represented the main fraction with alpha-bisabolol (42.8%) as the major constituent, making this plant a new and good source of this substance. Biological activity of the essential oil was evaluated against several bacterial and fungal strains as well as larvae from Aedes aegypti. The highest activity was found against Staphylococcus aureus, Pseudomonas aeruginosa, Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrum with MIC values from 32 to 125 microg/ml. The essential oil also showed potent inhibitory and bactericidal activities against three H. pylori strains, with MIC and MBC values of 62.5 microg/ml, and caused 100% mortality of A. aegypti larvae at a concentration of 500 microg/ml. Composition and biological activity of the essential oil from leaves of Plinia cerrocampanensis, a new source of alpha-bisabolol. Vila R, Santana AI, Pérez-Rosés R, Valderrama A, Castelli MV, Mendonca S, Zacchino S, Gupta MP, Cañigueral S. No
20030083 OBJECTIVE: To study the chemical constituents of the twigs of Garcinia xipshuanbannaensis.METHOD: The compounds were isolated by column chromatography with silica gel, RP-18 and Sephadex LH-20, and their structures were elucidated by spectroscopic analysis.RESULT: Fifteen compounds were obtained and identified, which were bannaxanthone E (1), xanthochymol (2), isoxanthochymol (3), cycloxanthochymol (4), osajaxanthone (5), gentisein (6), mangostinone (7), kaempferol (8), quercetin (9), vitexin (10), 2"-O-acetylvitexin (11), 3-acetoxyoleanolic acid (12), (-)-epicatechin (13), beta-sitosterol (14) and daucosterol (15), respectively.CONCLUSION: Compounds 4-9 and 11-13 were isolated from the plant and compounds 11-13 were obtained from the genus Garcinia for the first time. [Chemical constituents from twigs of Garcinia xipshuanbannaensis]. Na Z, Xu Y. No
20030409 Korean ginseng (Panax ginseng C.A. Meyer) has been extensively used as a functional food for thousands of years. This study with the aim to evaluate the potential of P. ginseng flower components as a functional food with medicinal properties resulted in the identification of three new dammarane-type saponins, named floralginsenosides Ka-Kc (1-3), along with seventeen known ones (4-20). Their structures were elucidated on the basis of chemical and spectroscopic methods, and their antioxidant activities were evaluated by the intracellular ROS radical scavenging DCF-DA assay. Among them, floralginsenoside Ka (1) displayed potent scavenging activity with the inhibition value of 64% at 10 microM; and ginsenoside Rb(1) (13), floralginsenoside Kc (3), floralginsenoside Kb (2), vinaginsenoside R(9) (11), majoroside F(1) (12), ginsenoside I (17), and ginsenoside II (18) showed moderate scavenging capacity with the inhibition rate of 28, 33, 35, 35, 35, 38, and 38% at 10 microM, respectively. These results warrant further studies concerning the potential of saponin extracts of P. ginseng flowers for functional foods. Dammarane-type saponins from the flower buds of Panax ginseng and their intracellular radical scavenging capacity. Tung NH1, Song GY, Nhiem NX, Ding Y, Tai BH, Jin LG, Lim CM, Hyun JW, Park CJ, Kang HK, Kim YH. No
20041704 Nineteen monoterpenoid indole alkaloids including seven new ones, melodinines A-G (1-7), were isolated from Melodinus henryi. The structures of the new compounds were elucidated using spectroscopic methods, and the structure of compound 4 was confirmed by single-crystal X-ray diffraction analysis. The known compounds were identified by comparing their spectroscopic data with those reported in the literature. All of the compounds were evaluated for cytotoxic activity against five human cancer cell lines, and compound 11 exhibited cytotoxicity against HL-60, SMMC-7721, A-549, and SK-BR-3 cells with IC50 values of 2.0, 16.8, 25.9, and 24.7 microM, respectively. Melodinines A-G, monoterpenoid indole alkaloids from Melodinus henryi. Feng T, Cai XH, Liu YP, Li Y, Wang YY, Luo XD. No
20050684 Two new quassinoids, javanicolide E (1) and javanicolide F (2), along with fifteen known C-20 quassinoids were isolated from the seeds of Brucea javanica (L.) Merr. The antitobacco mosaic virus (TMV) activity of these quassinoids was screened by the conventional half-leaf and leaf-disk method along with Western blot analysis. All of the seventeen quassinoids showed potent anti-TMV activity. Among them, eight compounds, brusatol (3), bruceine B (4), bruceoside B (5), yadanzioside I (6), yadanzioside L (7), bruceine D (8), yadanziolide A (9), and aglycone of yadanziolide D (17), showed strong antiviral activities, with IC(50) values in the range of 3.42-5.66 microM, and were much more effective than the positive control, ningnanmycin (IC(50) = 117.3 microM). The antiviral structure-activity relationships of quassinoids against TMV were also discussed. Anti-tobacco mosaic virus (TMV) Quassinoids from Brucea javanica (L.) Merr. Yan XH1, Chen J, Di YT, Fang X, Dong JH, Sang P, Wang YH, He HP, Zhang ZK, Hao XJ. No
20055135 Chemical constituents of the roots and stem of Salacia hainanensis Chun et How were isolated and purified with column chromatography on silica gel, Sephadex LH-20 and preparative HPLC. Their structures were elucidated based on physicochemical and spectral spectroscopic analysis. Depending on the activities of anti-alpha-glucosidase and inhibiting AGEs (advanced glycation end products, AGEs) formation in vitro, nine compounds were identified as 26, 27-dihydroxy-7, 24-tirucalladien-3-one (1), abruslactone A (2), lupeol (3), 21alpha, 30-dihydroxy-D: A-friedooleanan-3-one (4), 15alpha-hydroxyfriedelan-3-one (5), friedelin (6), mangiferin (7), epicatechin (8) and beta-sitosterol (9), separately. Among them, compound 1 is a new compound, and compound 2 was isolated from the Salacia genus for the first time, while, compounds 3, 4, 5, 8 were obtained from this plant for the first time. [A new trincallane derivative from Salacia hainanensis Chun et How]. Guo ZH, Xi RG, Wang XB, Wu LJ, Gao HY. No
20067016 OBJECTIVE: To investigate antitumor constituents from n-butyl alcohol extract of Alternanthera philoxeroides.METHOD: The constituents were isolated with silica gel, gel permeation chromatography, and purified by HPLC. Their structures were elucidated by spectroscopy and acid hydrolysis. The antitumor effects of extracts and isolated compounds were tested by MTH method in vitro.RESULT: Seven compounds were isolated and elucidated as followings: oleanolic acid 3-O-beta-D-glucuronopyranoside (1), oleanolic acid 28-O-beta-D-glucopyranoside (2), oleanolic acid 3-O-beta-D-glucuronopyranoside-6'-O-methyl ester (3), chikusetsusaponin IV a methyl ester (4), hederagenin 3-O-beta-D-glucuronopyranoside-6'-O-methyl ester (5), 4,5-dihydroblumenol (6), 6S,7E,9R-6,9-di-hydroxymegastigma-4,7-dien-3-one-9-O-beta-D-glucopyranoside (7). Compound 1 showed significant inhibitory effect against Hela and L929 with inhibitive ratios 91.3% and 92.9% at 30 mg x L(-1), respectively.CONCLUSION: Compounds 4, 5 and 7 were isolated from this genus for the first time. Compound 1 showed significant inhibitory effect against Hela and L929 at 30 mg x L(-1) [Constituents from Alternanthera philoxeroides and their antitumor activity]. Fang J, Chen J, Liu Y, Duan H. No
20069904 OBJECTIVE: To study the chemical constituents in the active portion from the flowers of Polygonum oriental.METHOD: Chromatographic techniques were employed for isolation and purification of the constituents and their structures were determined by spectral analysis and chemical evidence.RESULT: Nine compounds were obtained and identified as alphitonin (1), methyl 3, 4-dihydroxybenzoate (2), apocynin (3), kaempferol-3-O-beta-D-glucoside (4), 1,3,5-trihydroxybenzene (5), 3,3'-dimethoxyellagic-acid-4-O-beta-D-glucoside (6), kaempferol-3-O-alpha-L-rhamnoside (7), quercetin-3-O-alpha-L-rhamnoside (8), kaempferol (9).CONCLUSION: Compounds 2, 4, 5 were isolated from P. oriental for the first time and compounds 1, 3 were firstly obtained from the genus Polygonum. [Chemical constituents of flowers from Polygonum orientale]. Li Y, He X, Liu Z, Huang Y, Lan Y, Wang A, Wang Y. No
20078113 Three novel sesquiterpenoid-based meroterpenoids of psidials A-C (1-3) have been isolated from the leaves of Psidium guajava L. Their complete structures were elucidated by spectral and chemical methods, and that of 1 was confirmed by single-crystal X-ray diffraction analysis. Psidial B (2) and C (3) represented the new skeleton of the 3,5-diformylbenzyl phloroglucinol-coupled sesquiterpenoid. A possible biosynthetic pathway for 2-3 was postulated. 2-3 showed activity to enzyme PTP1B in 10 microM Psidials A-C, three unusual meroterpenoids from the leaves of Psidium guajava L. Fu HZ, Luo YM, Li CJ, Yang JZ, Zhang DM. No
20079815 Chemical investigation of the stems of Erythrina fusca Lour. led to the isolation of three new pterocarpans, named fuscacarpans A-C (1-3), together with fourteen known compounds, sandwicensin (4), erythribyssin A (5), erythrabissin I (6), demethylmedicarpin (7), eryvarin D (8), erypoegin I (9), hydroxycristacarpone (10), orientanol A (11), scandenone (12), genistein (13), liquiritigenin (14), isoliquiritigenin (15), vestitone (16) and 3,7,4'-trihydroxyflavone (17). Structures 1-3 were elucidated by spectroscopic and chemical methods. The isolates were evaluated for antibacterial, antiplasmodial and cytotoxic activities. Fuscacarpans A-C, new pterocarpans from the stems of Erythrina fusca. Innok P1, Rukachaisirikul T, Phongpaichit S, Suksamrarn A. No
20087473 We evaluated the in vitro antioxidant property and phytochemical constituents of the aqueous crude leaf extract of Helichrysum pedunculatum. The scavenging activity on superoxide anions, DPPH, H(2)O(2), NO and ABTS; and the reducing power were determined, as well as the flavonoid, proanthocyanidin and phenolic contents of the extract. The extract exhibited scavenging activity towards all radicals tested due to the presence of relatively high total phenol and flavonoids contents. Our findings suggest that H. pedunculatum is endowed with antioxidant phytochemicals and could serve as a base for future drugs. Phytochemical screening and polyphenolic antioxidant activity of aqueous crude leaf extract of Helichrysum pedunculatum. Aiyegoro OA, Okoh AI. PMC2808019
20097393 Megastigmane glycosides (1-5) together with seven (6-12) related known compounds were isolated from the whole plants of Gynostemma pentaphyllum. The structures were elucidated by means of spectroscopic methods, including 2D NMR, HR-ESIMS, and circular dichroism (CD), as well as chemical transformations to be (3R,4R,5S,6S,7E)-3,4,6-trihydroxymegastigmane-7-en-9-one-3-O-beta-D-glucopyranoside (gynostemoside A, 1), (3S,4S,5R,6R,7E,9R)-3,4,6,9-tetrahydroxymegastigmane-7-en-3-O-beta-D-glucopyranoside (gynostemoside B, 2), (3S,4S,5S,6S,7E,9R)-3,4,9-trihydroxymegastigmane-7-en-9-O-beta-D-glucopyranoside (gynostemoside C, 3), (3S,4S,5S,6S,7E,9R)-3,4,9-trihydroxymegastigmane-7-en-3-O-beta-D-glucopyranoside (gynostemoside D, 4), and (3S,4S,5S,6S,7E,9R)-3,4,9-trihydroxymegastigmane-7-en-4-O-beta-D-glucopyranoside (gynostemoside E, 5), respectively. Gynostemosides A-E, megastigmane glycosides from Gynostemma pentaphyllum. Zhang Z1, Zhang W, Ji YP, Zhao Y, Wang CG, Hu JF. No
20112712 OBJECTIVE: To study the chemical constituents of Flos Chrysanthemi Indici.METHODS: Chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were indentified by means of physicochemical and spectral data.RESULTS: From the 80% ethanol extract of the material, seven compounds were isolated. Their structures were identified as luteolin (1), luteolin-7-O-beta-D-glucopyranoside (2), luteolin-7-O-(6"-O-acetyl)-beta-D-glucopyranoside (3), diosmetin (4), diosmetin-7-O-beta-D-glucopyranoside (5), eupatilin (6) and apigenin (7), respectively.CONCLUSION: Compounds 3-6 are isolated from Flos Chrysanthemi Indici for the first time. [Studies on flavones from Flos Chrysanthemi Indici]. Tang Y, Gao MH, Yao MC. No
20112713 OBJECTIVE: To study the chemical constituents from Clematis brevicaudata.METHODS: The compounds were isolated by column chromatography and their structures were elucidated through spectroscopic analysis (NMR).RESULTS: Eight compounds were isolated and identified as: palmitic acid (1), 1-docosanol (2), pentacosanoic acid-2', 3'-dihydroxypropyl ester (3), beta-sitosterol (4), daucosterol (5), a mixture of the trans-p-coumarate of the n-alkanols (6), 3,4-dihydroxy-trans coumatate ethyl ester (7), syringaresinol-O-D-glucopyranoside (8).CONCLUSION: All these compounds are obtained from Clematis brevicaudata for the first time. [Study on the chemical constituents from Clematis brevicaudata]. Yang AM, Du J, Miao ZH, Yuan HJ. No
20161915 The methanolic leaf extract of Newbouldia laevis was subjected to preliminary phytochemical screening and in-vitro antimicrobial tests. The extract revealed the presence of flavonoids, tannins, terpenes, steroidal and cardiac glycosides. The antimicrobial activity of the plant extract was assayed by the agar plate disc diffusion and nutrient broth dilution techniques. Test microorganisms were Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Klebsiella spp. and Candida albicans; all the organisms were laboratory isolates. The extract inhibited the growth of all the test organisms especially against Klebsiella spp. and S. aureus which had mean inhibition zone of 42.3+/-1.5 and 32.3+/-1.5 mm respectively. The results showed minimum inhibitory concentration (MIC) of 1.563 mg/ml against Escherichia coli and Klebsiella spp. and 3.125 mg/ml against Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhi. The minimal bactericidal concentration (MBC) against Escherichia coli and Staphylococcus aureus was 0.39 mg/ml. This study has justified the traditional use of this plant for the treatment of stomach discomfort, diarrhea, dysentery and as a remedy for wound healing whose causative agents are some of the organisms used in this study. Phytochemical and in vitro antimicrobial assay of the leaf extract of Newbouldia laevis. Usman H, Osuji JC. No
20161916 Anthocephalus cadamba (Roxb.) Miq. Syn A. chinensis (Lamk) A. Rich (Rubiaceae) is ethnomedicinally widely used in the form of paste by tribe in western Ghats for treating skin diseases. In this context, antimicrobial potential of A. cadamba against a wide range of microorganisms was studied. To validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing. The alchoholic and aqueous extract of this plant showed significant antibacterial and antifungal activity against almost all the organisms: Micrococcus luteus, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and four fungi Candida albicans, Trichophyton rubrum--dermatophyte fungi, Aspergillus niger, Aspergillus flavus and Aspergillus nidulans--systemic fungi, with especially good activity against the dermatophyte (Trichophyton rubrum) and some infectious bacteria (Escherichia coli, Proteus mirabilis and Staphylococcus aureus) with an MIC of 2.5 microg/disc. The results show that A. cadamba extract has potent wound healing capacity as shown from the wound contraction and increased tensile strength. The results also indicated that A. cadamba extract possesses potent antioxidant activity by inhibiting lipid peroxidation and increase in the superoxide dismutase (SOD) and catalase activity. Antimicrobial, wound healing and antioxidant activities of Anthocephalus cadamba. Umachigi SP, Kumar GS, Jayaveera K, Kishore KD, Ashok KC, Dhanapal R. PMC2816507
20161918 Four plants (Amphimas pterocaroides, Harungana madagascariensis, Myrianthus arboreus, and Cussonia barteri) that are commonly used in Cameroon for the management or reversal of anaemia were screened for their phytochemical content and antioxidant potential. Four extracts (methanolic, hydro-ethanolic, aqueous, and hydrolysed) from each of the plants were prepared and analysed. Qualitative phytochemical tests were used to detect the presence of alkaloids, tannins, saponins, flavonoids, glycosides and phenols, while three quantitative methods; Folin, Ferric Reducing Antioxidant Power (FRAP) and diphenyl-1, 2-picryl hydrazyl (DPPH) were used to determine the antioxidant potential of these extracts. With the exception of the extracts of Cussonia barteri (negative for triterpenes and phenols) and the aqueous extract of Harungana madagascariensis (negative result for cardiac glycosides and glycosides), all other extracts contained the phytochemicals tested. The highest antioxidant activities were observed in the hydrolysed extracts of each plant, while the aqueous extract showed the least activity irrespective of the method used. The presence of active phytochemical substances with antioxidant activities may provide substantial basis for the use of these plants in ethnomedicine. Phytochemical studies and antioxidant properties of four medicinal plants used in Cameroon. Biapa PC, Agbor GA, Oben JE, Ngogang JY. PMC2816508
20161952 Malaria remains one of the leading public health problems in Cameroon as in other parts of Sub-Saharan Africa. In the past decades, this situation has been aggravated by the increasing spread of drug-resistant Plasmodium falciparum strains. New antimalarial drug leads are therefore urgently needed. Traditional healers have long used plants to prevent or cure infections. This article reviews the current status of botanical screening efforts in Cameroon as well as experimental studies done on antimalarial plants. Data collected from 54 references from various research groups in the literature up to June 2007 shows that 217 different species have been cited for their use as antimalarials in folk medicine in Cameroon. About a hundred phytochemicals have been isolated from 26 species some among which are potential leads for development of new antiamalarials. Crude extracts and or essential oils prepared from 54 other species showed a wide range of activity on Plasmodium spp. Moreover, some 137 plants from 48 families that are employed by traditional healers remain uninvestigated for their presumed antimalarial properties. The present study shows that Cameroonian flora represents a high potential for new antimalarial compounds. Further ethnobotanical surveys and laboratory investigations are needed to fully exploit the potential of the identified species in the control of malaria. The antimalarial potential of medicinal plants used for the treatment of malaria in Cameroonian folk medicine. Titanji VP, Zofou D, Ngemenya MN. PMC2816552
20162089 Ten Nigerian plants suggested from their ethnomedical uses to possess antimicrobial and antioxidant activities were studied for their anti-microbial and anti-oxidant properties. Antimicrobial activity was tested against Escherichia coli NCTC 10418, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Candida albicans, Candida pseudotropicalis and Trichophyton rubrum (clinical isolate). Trichilia heudelotti leaf extract showed both antibacterial and antifungal activities and was the most active against all the strains of bacteria tested. Boerhavia diffusa, Markhamia tomentosa and T. heudelotti leaf extracts inhibited the gram negative bacteria E. coli and P. aeruginosa strains whereas those of M. tomentosa, T. heudelotti and Sphenoceutrum jollyamum root inhibited at least one of the fungi tested. At a concentration of 312 microg/ml, hexane and chloroform fractions of T. heudelotti extract inhibited 6 and 14% of the fifty multi-drug resistant bacteria isolates from clinical infections, respectively. At < or = 5 mg/ml, the CHCl(3) (64%) and aqueous (22%) fractions of T. heudelotti and those of CHCl(3) (34%) and EtOAC (48%) of M. tomentosa gave the highest inhibition that was stronger than their corresponding methanol extracts. The corresponding EC(50) of the extracts on M. acuminata, T. heudelotti, E. senegalensis and M. tomentosa were 4.00, 6.50, 13.33, and 16.50 ig/ml using the TLC staining and 1,1-dipheyl-2-picry-hydrazyl (DPPH) free radical scavenging assay. Therefore, leaf extracts of M. tomentosa and T. heudelotti, especially the latter, possess strong antimicrobial and antioxidant activities and should be further investigated. These activities justified the ethnomedical uses of these plants. Antimicrobial and antioxidant activities of some Nigerian medicinal plants. Aladesanmi AJ, Iwalewa EO, Adebajo AC, Akinkunmi EO, Taiwo BJ, Olorunmola FO, Lamikanra A. PMC2816440
20176087 Two new prenylated xanthones and a new prenylated tetrahydroxanthone, garcimangosxanthone A-C (1-3), along with fourteen known xanthones were isolated from the pericarp of Garcinia mangostana. Their structures were elucidated on the basis of spectroscopic data. Compounds 1 and 2 exhibited in vitro cytotoxicity against A549, LAC and A375 cell lines with IC(50) values of 5.7-24.9 microM, which were comparable to those of doxorubicin. Two new prenylated xanthones and a new prenylated tetrahydroxanthone from the pericarp of Garcinia mangostana. Zhang Y, Song Z, Hao J, Qiu S, Xu Z No
20183255 A new farnesylated flavonol derivative, macagigantin (1), together with two known flavonoids, glyasperin A (2) and apigenin (3), had been isolated from the acetone extract of the leaves of Macaranga gigantea. The structure of the new compound was elucidated as 6-farnesylkaempferol based on its spectroscopic data, including UV, IR, 1D and 2D NMR, and HR-EI-MS spectra. Compounds 1-3 were evaluated for their cytotoxic properties against P-388 cells, their IC(50) values being 11.3, 6.0, and 5.1 microM, respectively. Macagigantin, a farnesylated flavonol from Macaranga gigantea. Tanjung M, Hakim EH, Mujahidin D, Hanafi M, Syah YM. No
20183302 Two new triterpenoids taraxer-14-ene-1alpha,3beta-diol (1) and 3beta-hydroxytaraxer-14-ene-1-one (2), together with the known triterpenes taraxerol (3), betulin (4), betulinic acid (5), sumaresinolic acid (6), and 5-hydroxy-2-methoxy-1,4-naphthoquinone (7), 5,7-dihydroxy-6,8-dimethylchromone (8), alpha-monpalmitin (9), palmitic acid (10), 6beta-hydroxystigmast-4-en-3-one (11), beta-sitostero1 (12), have been isolated from the petroleum ether fraction of the ethanolic extract of Pterospermum heterophyllum. Their structures were established by spectroscopic methods including IR, MS, 1D, and 2D NMR experiments. Compounds 1-8 were evaluated against several human cancer cell lines. Compound 1 showed in vitro selective cytotoxicity against human lung cancer cell lines (A549) with an IC(50) value of 1.22 microM. Compound 7 showed significant cytotoxicity against the A549, HCT-8, Bel7402, BGC-823, and A2780 cancer cell lines with IC(50) values of 0.21, 0.55, 0.40, 0.59, and 0.34 microM, respectively. However, the other compounds were inactive (IC(50)>10 microM). Triterpenoids from the roots of Pterospermum heterophyllum Hance. Li S, Shi Y, Shang XY, Cui BS, Yuan Y, Chen XG, Yang YC, Shi JG. No
20183333 Two new triterpenoid saponins, incarvillosides A (1) and B (2), and two new monoterpenoid glycosides, incarvillosides C (3) and D (4), were isolated from the high-polarity fraction of the whole plant of Incarvillea delavayi. By means of spectroscopic data and chemical degradation, the structures were established as (3beta,21beta)-3,19,21,23-tetrahydroxyurs-12-en-28-oic acid 28-O-beta-d-glucopyranoside (1), (2beta,3beta,19alpha)-2,3,19,23-tetrahydroxyolean-12-en-28-oic acid 28-O-beta-d-glucopyranoside (2), (2S,6R)-2,6-dimethyl-1,8-octanediol 1-O-beta-d-glucopyranoside (3), and (2S,6R)-2,6-dimethyl-1,8-octanediol 8-O-beta-d-glucopyranoside (4). Triterpenoid saponins and monoterpenoid glycosides from Incarvillea delavayi. Ge X, Lin DC, Zhang WD, Zhang XR. No
20184022 Chemical investigation of Andrographis paniculata Nees (Acanthaceae) resulted in the isolation of three flavones identified as 5-hydroxy-7,8,2'-trimethoxyflavone 1, 5-hydroxy-7,8-dimethoxyflavone 2 and 5-hydroxy-7,8,2',5'-tetramethoxyflavone 3 from the hexane, methanol and chloroform extracts of the root and stem. Flavones from the stem of Andrographis paniculata Nees. Radhika P, Prasad YR, Lakshmi KR. No
20184037 The essential oil compositon of Achillea clusiana Tausch from Bulgaria has been studied by GC and GC/MS. Fifty-four components were registered, representing 92.5% of the oil. The oil was characterized by the presence of oxygenated mono- and sesquiterpenoids. The main components were beta-thujone (17.2%), 1,8-cineole (11.2%), camphor (11.1%) and alpha-thujone (7.8%). Farnesol (3.1%), nerolidol (2.7%) and oxygenated nerolidol derivatives (cabreuva oxides A-D, isohumbertiols A-D, bejarol and 7-hydroxy-6,7-dihydro-5,6E-dehydronerolidol) were the main sesquiterpenoids in the oil. Essential oil composition of Achillea clusiana from Bulgaria. Trendafilova A, Todorova M, Vitkova A. No
20190446 Two new xanthones, namely pruniflorone K (1) and L (2), have been isolated from the roots of Cratoxylum formosum ssp. pruniflorum, along with thirteen known xanthones (3-15). Their structures were mainly established using the spectroscopic methods. Only isolated compounds with sufficient amount were evaluated for antibacterial and antifungal activities. Chromene and prenylated xanthones from the roots of Cratoxylum formosum ssp. pruniflorum. Boonnak N, Karalai C, Chantrapromma S, Ponglimanont C, Kanjana-Opas A, Chantrapromma K, Kato S. No
20209910 OBJECTIVE: To investigate the chemical constituents of the extract of Galium verum.METHOD: The compounds were isolated by chromatography and identified by spectral data.RESULT: The eleven compounds obtained were identified as (+)-pinoresinol 4,4'-O-bis-beta-D-glucopyranoside (1), epipinoresinol (2), (+) -medioresinol (3), isorhamnetin (4), isorhamnetin 3-O-alpha-L-rhamnopyranosyl-(1-6)-beta-D-glucopyranoside (5), diosmetin (6), diosmetin 7-O-beta-D-glucopyranoside (7), quercetin-3-O-beta-D-glucopyranoside (8), ursolic acid (9), ursolic aldehyde (10) and rubifolic acid (11).CONCLUSION: Compounds 1-5 and 9-11 were isolated from this genus for the first time. [Chemical constituents of Galium verum]. Zhao C, Shao J, Cao D, Zhang Y, Li X. No
20334149 The composition and antimicrobial activity of the essential oil from the underground parts (roots and rhizomes) of Laserpitium zernyi Hayek (Apiaceae) were investigated. The pale blue essential oil, obtained by hydrodistillation, was analyzed by GC and GC-MS. Forty-three compounds were identified (94.3% of total oil). The main constituent, beside alpha-pinene (31.6%) was alpha-bisabolol (30.9%), so this oil may be a novel potential natural source of this sesquiterpene alcohol. The antimicrobial activity was tested using the microdilution method against Gram (+) bacteria (Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Enterococcus faecalis), Gram (-) bacteria (Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli), and two strains of yeast (Candida albicans). L. zernyi oil showed significant antibacterial activity against S. epidermidis, S. aureus and M. luteus, but lower activities against the other tested strains. Essential oil from the underground parts of Laserpitium zernyi: potential source of alpha-bisabolol and its antimicrobial activity. Popović V, Petrović S, Pavlović M, Milenković M, Couladis M, Tzakou O, Duraki S, Niketić M. No
20335930 Chemical investigation of the EtOH extract of the twigs of Calophyllum inophyllum collected in Hainan Province of China resulted in the isolation of two new prenylated xanthones, caloxanthone O (1) and caloxanthone P (2). Their structures were elucidated by a study of their physical and spectral data. Compound 1 exhibited cytotoxicity against human gastric cancer cell line (SGC-7901),with an IC50 value of 22.4 microg mL-1. Caloxanthones O and P: two new prenylated xanthones from Calophyllum inophyllum. Dai HF, Zeng YB, Xiao Q, Han Z, Zhao YX, Mei WL. No
20336209 Argyreia speciosa commonly known as Vridha daraka in Sanskrit is one of the important plants used in indigenous system of medicine. The root is regarded as an alternative tonic and useful in the diseases of nervous system. To confirm the veracity of aforementioned claim, we have evaluated the anticonvulsant effect of the extract. In this investigation, the mice were pretreated with different doses of Argyreia speciosa extract (100, 200, 400 mg/kg) for 10 days and then, they were subjected to either pentylenetetrazole (80 mg/kg) or maximal electroshock seizures (50 mA, 0.2 s) treatment. The hydroalcoholic extract of Argyreia speciosa at the dose of 200 and 400 mg/kg significantly delayed the latency to the onset of first clonus as well as onset of death in unprotected mice and exhibited protection in 16.66% and 33.33% of pentylenetetrazole treated mice respectively. Whereas in case of maximal electroshock-seizures, the dose of 200 and 400 mg/kg significantly reduced the duration of hind limb extension and both the doses were statistically found to be equipotent. The reference standards, clonazepam (0.1 mg/kg) and phenytoin (20 mg/kg) provided complete protection. Thus, present study revealed anticonvulsant effect of Argyreia speciosa against pentylenetetrazole- and maximal electroshock-induced convulsions in mice. Anticonvulsant Activity of Argyreia speciosa in Mice. Vyawahare NS, Bodhankar SL. PMC2839397
20336211 Seventeen Indian folklore medicinal plants were investigated to evaluate antibacterial activity of aqueous, ethanol and acetone extracts against 66 multidrug resistant isolates of major urinary tract pathogens (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Enterococcus faecalis) by disc diffusion method. Ethanol extract of Zingiber officinale and Punica granatum showed strong antibacterial activity against Escherichia coli. Ethanol extracts of Terminalia chebula and Ocimum sanctum exhibited antibacterial activity against Klebsiella pneumoniae. Ethanol extract of Cinnamomum cassia showed maximum antibacterial activity against Pseudomonas aeruginosa while ethanol extract of Azadirachta indica and Ocimum sanctum exhibited antibacterial activity against Enterococcus faecalis. The results support the folkloric use of these plants in the treatment of urinary tract infections by the tribals of Mahakoshal region of central India. Antibacterial Activity of Medicinal Plants Against Pathogens causing Complicated Urinary Tract Infections. Sharma A, Chandraker S, Patel VK, Ramteke P. PMC2839399
20336224 Abstarct"OBJECTIVES:The purpose of the present study was to investigate the anti-inflammatory properties of aqueous extract of the leaves of H. integrifolia, Planch.MATERIALS AND METHODS:The hind paw edema was produced in rats by subplanter injection of carageenan. The aqueous extract of H. integrifolia, Planch. (AHI) at dose (250 and 500 mg/kg p.o) was given to observe % inhibition of paw edema which were comparable with indomethacin (10 mg/kg p.o) used as a reference drug.RESULTS:The extract administered orally at doses of 250 and 500 mglkg p.o produced a significant (P < 0.05) dose dependent inhibition of edema formationCONCLUSIONS:A significant % inhibition of paw edema by the aqueous extract of leaves of H. integrifolia, Planch. and its almost nearby same % inhibition with indomethacin suggest its usefulness as an anti-inflammatory agent." Studies on anti-inflammatory effect of aqueous extract of leaves of Holoptelea integrifolia, Planch. in rats Sharma S, Lakshmi KS, Patidar A, Chaudhary A, Dhaker S. PMC2841239
20354804 New benzophenone and flavonol galloyl glycosides were isolated from an 80% MeOH extract of Psidium guajava L. (Myrtaceae) together with five known quercetin glycosides. The structures of the novel glycosides were elucidated to be 2,4,6-trihydroxybenzophenone 4-O-(6''-O-galloyl)-beta-D: -glucopyranoside (1, guavinoside A), 2,4,6-trihydroxy-3,5-dimethylbenzophenone 4-O-(6''-O-galloyl)-beta-D: -glucopyranoside (2, guavinoside B), and quercetin 3-O-(5''-O-galloyl)-alpha-L: -arabinofuranoside (3, guavinoside C) by NMR, MS, UV, and IR spectroscopies. Isolated phenolic glycosides showed significant inhibitory activities against histamine release from rat peritoneal mast cells, and nitric oxide production from a murine macrophage-like cell line, RAW 264.7. New benzophenone and quercetin galloyl glycosides from Psidium guajava L. Matsuzaki K, Ishii R, Kobiyama K, Kitanaka S. No
20390751 Two new xanthones, pyranocycloartobiloxanthone A (1) and dihydroartoindonesianin C (2), were isolated from the stem bark of Artocarpus obtusus Jarrett by chromatographic separation. Their structures were determined by using spectroscopic methods and comparison with known related compounds. Pyranocycloartobiloxanthone A (1) showed strong free radical scavenging activity by using DPPH assay as well as cytotoxicity towards K562, HL-60, and MCF7 cell lines. Two new xanthones from Artocarpus obtusus. Hashim N, Rahmani M, Sukari MA, Ali AM, Alitheen NB, Go R, Ismail HB. No
20428067 In our screening program for new agrochemicals from local wild plants, Artemisia capillaris and A. mongolica were found to possess insecticidal activity against the maize weevil, Sitophilus zeamais. The essential oils of aerial parts of the two plants were obtained by hydrodistillation and were investigated by GC and GC-MS. The main components of A. capillaris essential oil were 1,8-cineole (13.75%), germacrene D (10.41%), and camphor (8.57%). The main constituents of A. mongolica essential oil were alpha-pinene (12.68%), germacrene D (8.36%), and gamma-terpinene (8.17%). Essential oils of A. capillaris and A. mongolica possess fumigant toxicity against S. zeamais adults with LC50 values of 5.31 and 7.35 mg/L respectively. The essential oils also show contact toxicity against S. zeamais adults with LD50 values of 105.95 and 87.92 microg/adult, respectively. Chemical composition and insecticidal activity against Sitophilus zeamais of the essential oils of Artemisia capillaris and Artemisia mongolica. Liu ZL, Chu SS, Liu QR. No
20432153 Gas chromatography and gas chromatography/mass specteometry analyses of root volatiles of Achillea distans Willd. subsp. distans, collected from wild populations in Serbia, enabled the identification of 185 constituents, accounting for 93.6% of the total oil. Main constituents of the oil were tau-cadinol (17.6%), alismol (14.1%), alpha-cadinol (9.1%) and caryophyllene oxide (5.0%). The root oil was additionally characterised by the presence of five different amides containing the olefinic C(10) acid moieties. This is the first report on A. distans subsp. distans root volatiles and on the occurrence of piperidides and piperideides as essential oil constituents. Composition of Achillea distans Willd. subsp. distans root essential oil. Lazarevic J, Radulovic N, Zlatkovic B, Palic R. No
20435124 Abstarct:"AIM OF THE STUDY:The aim of this study was to evaluate the antiplasmodial properties of 13 plants used against malaria in traditional medicine in Burkina Faso.MATERIALS AND METHODS:In vitro antiplasmodial activity of dichloromethane, methanol and aqueous crude extracts obtained from vegetal samples collected in Burkina Faso was first evaluated on the Plasmodium falciparum 3D7 chloroquine-sensitive strain using a colorimetric method. Evaluation of 13 selected medicinal plants from Burkina Faso for their antiplasmodial properties Jansen O, Angenot L, Tits M, Nicolas JP, De Mol P, Nikiéma JB, Frédérich M. No
20442821 Abstarct:"OBJECTIVES:Screening of the medicinal plants and determination of minimum inhibitory concentration (MIC) against Vibrio cholerae and Vibrio parahaemolyticus.MATERIALS AND METHODS:A simple in vitro screening assay was employed for the standard strain of Vibrio cholerae, 12 isolates of Vibrio cholerae non-O1, and Vibrio parahaemolyticus. Aqueous and organic solvent extracts of different parts of the plants were investigated by using the disk diffusion method. Extracts from 16 medicinal plants were selected on account of the reported traditional uses for the treatment of cholera and gastrointestinal diseases, and they were assayed for vibriocidal activities.RESULTS:The different extracts differed significantly in their vibriocidal properties with respect to different solvents. The MIC values of the plant extracts against test bacteria were found to be in the range of 2.5-20 mg/ml. Vibriocidal activity of certain medicinal plants used in Indian folklore medicine by tribals of Mahakoshal region of central India. Sharma A, Patel VK, Chaturvedi AN. PMC2861814
20460806 Three new dibenzocyclooctadiene lignans, schilancifolignans A-C (1-3), together with thirteen known ones, were isolated from the leaves and stems of Schisandra lancifolia. The structures of 1-3 were elucidated by spectroscopic methods, including extensive 1D- and 2D-NMR techniques. Compounds 1-3 were tested for their anti-human immunodeficiency virus-1 activities and showed weak bioactivities. Dibenzocyclooctadiene lignans from Schisandra lancifolia and their anti-human immunodeficiency virus-1 activities. Yang GY, Fan P, Wang RR, Cao JL, Xiao WL, Yang LM, Pu JX, Zheng YT, Sun HD. No
20462230 Four new bisindole alkaloids, melodinines H-K (1-4), a new monomer, melodinine L (5), and 11 known alkaloids were isolated from Melodinus tenuicaudatus. The structures of 1-5 were elucidated by extensive spectroscopic methods, and the known compounds were identified by comparison with data in the literature. All of the compounds were evaluated for their cytotoxicity against five human cancer cell lines. Alkaloids 1, 3, and 4 and the known compound 11-methoxytabersonine (8) exhibited inhibitory effects, with IC(50) values comparable to those of cisplatin and vinorelbine. Cytotoxic indole alkaloids from Melodinus tenuicaudatus. Feng T, Li Y, Wang YY, Cai XH, Liu YP, Luo XD. No
20462233 Twelve new dibenzocyclooctadiene lignans, marlignans A-L (1-12), together with 16 known compounds, were isolated from the leaves and stems of Schisandra wilsoniana. The structures of 1-12 were elucidated by spectroscopic methods including 1D- and 2D-NMR techniques. Compounds 1-12 were evaluated for their anti-HIV activities, of which compounds 3, 6, 8, and 12 showed modest activities with therapeutic index values of 13.2, 15.6, 17.6, and 16.4, respectively. Dibenzocyclooctadiene lignans from Schisandra wilsoniana and their anti-HIV-1 activities. Yang GY, Li YK, Wang RR, Li XN, Xiao WL, Yang LM, Pu JX, Zheng YT, Sun HD. No
20462416 BACKGROUND:There is an urgent need to identify new anti-malarial drug targets for both prophylaxis and chemotherapy, due to the increasing problem of drug resistance to malaria parasites. In the present study, the aim was to discover novel, effective plant-based extracts for the activity against malaria. In vitro and in vivo anti-malarial activity of Boerhavia elegans and Solanum surattense Ramazani A, Zakeri S, Sardari S, Khodakarim N, Djadidt ND. PMC2881073
20486077 A new C(14) pterosin sesquiterpenoid, named (2R)-pterosin P (1), and a new natural product, named dehydropterosin B (3), were isolated from the aerial parts of Pteris multifida Poir., along with nine known compounds (2, 4-11). By chiral HPLC, compounds 1 and 2 were isolated as a pair of enantiomeric pterosin sesquiterpenoids. The planar structure of 1 was elucidated on the basis of NMR spectroscopy analysis, and the absolute configuration was established by the CD spectrum. In addition, the absolute structure of 1 was further confirmed by single-crystal X-ray diffraction (CuK α). Compounds 3, 5, and 6 showed potent cytotoxicity against PANC-1 (human pancreatic cancer) and NCI-H446 (human small-cell lung cancer) cell lines, with IC(50) values in the range of 4.27-14.63 µM. Pterosins from Pteris multifida. Ouyang DW, Ni X, Xu HY, Chen J, Yang PM, Kong DY. No
20488223 Essential oil extracted by hydrodistillation from Tunisian variety of Cuminumcyminum was characterized by means of GC and GC-MS. Twenty-one components were identified and C. cyminum contained cuminlaldehyde (39.48%), gamma-terpinene (15.21%), O-cymene (11.82%), beta-pinene (11.13%), 2-caren-10-al (7.93%), trans-carveol (4.49%) and myrtenal (3.5%) as a major components. Moreover, C. cyminum oil exhibited higher antibacterial and antifungal activities with a high effectiveness against Vibrio spp. strains with a diameter of inhibition zones growth ranging from 11 to 23 mm and MIC and MBC values ranging from (0.078-0.31 mg/ml) to (0.31-1.25mg/ml), respectively. On the other hand, the cumin oil was investigated for its antioxidant activities using four different tests then compared with BHT. Results showed that cumin oil exhibit a higher activity in each antioxidant system with a special attention for beta-carotene bleaching test (IC(50): 20 microg/ml) and reducing power (EC(50): 11 microg/ml). In the light of these findings, we suggested that C. cyminum essential oil may be considered as an interesting source of antibacterial, antifungal and antioxidants components used as potent agents in food preservation and for therapeutic or nutraceutical industries. Chemical composition and biological activities of Tunisian Cuminum cyminum L. essential oil: a high effectiveness against Vibrio spp. strains. Hajlaoui H, Mighri H, Noumi E, Snoussi M, Trabelsi N, Ksouri R, Bakhrouf A. No
20523878 Ten plants indigenous to Sudan and of common use in Sudanese folk-medicine, were examined in vitro for antimalarial activity against schizonts maturation of Plasmodium falciparum, the major human malaria parasite. All plant samples displayed various antiplasmodial activity. Three plant extracts caused 100% inhibition of the parasite growth at concentrations of plant material </= 500 ug/ml. The two most active extracts that produced 100% inhibition of the parasite growth at concentration of plant material </= 50 mug/ml were obtained from the seeds of Nigella sativa and the whole plant of Aristolochia bracteolata. The ten plants were phytochemically screened for their active constituents. The two most active plants showed the presence of sterols, alkaloids and tannins. Antiplasmodial Activity of Some Medicinal Plants Used in Sudanese Folk-medicine Ahmed el-HM, Nour BY, Mohammed YG, Khalid HS. PMC2879607
20540535 Seven new triterpene saponins, clematochinenosides A-G (1-7), together with 17 known saponins (8-24), were isolated from the roots and rhizomes of Clematis chinensis. Their structures were elucidated on the basis of spectroscopic evidence and hydrolysis products. Compounds 1, 3-7, and 20-24 showed inhibitory activities against COX-1 and COX-2 enzymes. Triterpene saponins from Clematis chinensis and their potential anti-inflammatory activity. Fu Q, Zan K, Zhao M, Zhou S, Shi S, Jiang Y, Tu P. No
20552523 The chemical composition of the essential oil from Heracleum rechingeri was analysed using GC-MS. Twenty-seven compounds, accounting for 94.62% of the extracted essential oil, were identified. The main oil compounds were octyl acetate (29.49%), elemicine (23.06%), (E)-caryophyllene (9.26%), caryophyllene oxide (6.42%), terpinolene (6.12%) and (Z)-3-octenyl acetate (4.72%). The antimicrobial activity of the essential oil was tested against three different bacteria by disc diffusion method and showed maximum inhibitory activity against Gram-positive bacteria, especially Bacillus subtilis. Chemical composition and antibacterial activity of essential oil of Heracleum rechingeri Manden from Iran. Habibi Z, Eshaghi R, Mohammadi M, Yousefi M. No
20570298 Three xanthones, named cratoxylumxanthones B-D (1-3), along with five known xanthones (4-8), were isolated from the stems of Cratoxylum cochinchinense (Lour.) Blume. Their structures were elucidated by interpretation of spectroscopic data. Among these xanthones, cochinxanthone D (4) exhibited the most potent antioxidant activity in both the DPPH radical scavenging and the lipid peroxidation inhibition assays. Xanthones from the stems of Cratoxylum cochinchinense. Udomchotphruet S, Phuwapraisirisan P, Sichaem J, Tip-Pyang S. No
20571171 Abstarct:"BACKGROUND & OBJECTIVES:Emergence of multi-drug resistant (MDR) and extensively-drug resistant (XDR) strains of Mycobacterium tuberculosis has further complicated the problem of tuberculosis (TB) control. Medicinal plants offer a hope for developing alternate medicines for the treatment of TB. The present study was done to evaluate in vitro anti-tubercular activity of five medicinal plants viz., Acalypha indica, Adhatoda vasica, Allium cepa, Allium sativum and Aloe vera.METHODS:Aqueous extracts of leaves of A. indica, A. vasica, bulbs of A. cepa, cloves of A. sativum and pure gel of A. vera leaves, were tested in vitro for their activity against two MDR isolates (DKU-156 and JAL-1236), reference susceptible strain M. tuberculosis H37Rv as well as rapid grower mycobacterial pathogen M. fortuitum (TMC-1529) using Lowenstein Jensen (L-J) medium and colorimetric BacT/ ALERT 3D system. Activity in L-J medium was evaluated by percentage inhibition which was calculated by mean reduction in number of colonies on extract containing as compared to extract free controls.RESULTS:Extracts of all the five plants A. indica, A. vasica, A. cepa, A. sativum and A. vera exhibited anti-tuberculosis activity in L-J medium, the proportion of inhibition of these plants extract in respect mentioned above is 95, 32, 37, 72, 32 per cent, respectively for MDR isolate DKU-156 and 68, 86, 79, 72, 85 per cent, respectively for another MDR isolate JAL-1236, while for sensitive M. tuberculosis H37Rv, inhibition was found to be 68, 70, 35, 63 and 41 per cent, at 4 per cent v/v concentration in L-J medium. There was no inhibition against rapid grower M. fortuitum (TMC-1529). In BacT/ALERT also, extracts of these plants showed significant inhibition against M. tuberculosis.INTERPRETATION & CONCLUSION:Our findings showed that all these plants exhibited activity against MDR isolates of M. tuberculosis. While the anti-TB activity of A. vera, A. vasica and A. sativum against MDR isolates confirm earlier results, activity of the extracts of A. indica and A. cepa is reported for the first time. Further studies aimed at isolation and identification of active substances from the extracts which exhibited promising activities, need to be carried out." Anti-tuberculosis activity of selected medicinal plants against multi-drug resistant Mycobacterium tuberculosis isolates. Gupta R, Thakur B, Singh P, Singh HB, Sharma VD, Katoch VM, Chauhan SV. No
20575413 OBJECTIVE: To study the chemical constituents from pine needles of Cedrus deodara.METHODS: Chemical constituents were isolated by silica gel and Sephadex LH-20 column chromatography. The structures of the isolated compounds were elucidated through spectroscopic analysis.RESULTS: The compounds were identified as 9-hydroxy-dodecanoic acid (I), ethyl laurate (II), ethyl stearate (III), 3beta-hydroxy-oleanolic acid methyl ester (IV), beta-sitosterol (V), shikimic acid (VI), methylconiferin (VII), ferulic acid beta-glucoside (VIII).CONCLUSION: Compounds I-IV, VI-VIII are isolated and identified from this genus for the first time, compound V is isolated from pine needles of this genus for the first time. [Study on the chemical constituents from pine needles of Cedrus deodara]. Zhang JM, Shi XF, Li C, Fan B, Wang DD, Liu DY. No
20576577 Eight new indole alkaloids, alpneumines A-H (1-8) were isolated from the Malaysian Alstonia pneumatophora (Apocynaceae) and their structures were determined by MS and 2D NMR spectroscopic methods. Alpneumines E and G (5 and 7), vincamine, and apovincamine showed anti-melanogenesis in B16 mouse melanoma cells. Alpneumines A-H, new anti-melanogenic indole alkaloids from Alstonia pneumatophora. Koyama K1, Hirasawa Y, Hosoya T, Hoe TC, Chan KL, Morita H. No
20593515 Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. Brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. As an alternative to conventional antibiotics, medicinal plants are valuable resources for new agents against antibiotic-resistant strains. The aim of this study was to investigate the usage of native plants for brucellosis treatment. For this purpose, the anti-brucella activities of ethanolic and methanolic extracts of Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, Plantago ovata, Cordia myxa, and Crocus sativus were assessed. The activity against a resistant Br. melitensis strain was determined by disc diffusion method at various concentrations from 50-400 mg/ml. Antibiotic discs were also used as a control. Among the evaluated herbs, six plant (Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, and Crocus sativus) showed anti-brucella activity. Oliveria decumbens was chosen as the most effective plant for further studies. A tested isolate exhibited resistance to tetracycline, nafcillin, oxacillin, methicillin, and colistin. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values for Oliveria decumbens against resistant Br. melitensis were the same (5 mg/ml), and for gentamicin they were both 2 mg/ml. Time-kill kinetics for a methanolic extract of Oliveria decumbens was 7 h whereas for an ethanolic extract it was 28 h. Also, Oliveria decumbens extracts showed a synergistic effect in combination with doxycycline and tetracycline. In general, the similar values of MIC and MBC for Oliveria decumbens suggest that these extracts could act as bactericidal agents against Br. melitensis. In addition to Oliveria decumbens, Crocus sativus and Salvia sclarea also had good anti-brucella activity and these should be considered for further study. In vitro assay for the anti-Brucella activity of medicinal plants against tetracycline-resistant Brucella melitensis. Motamedi H, Darabpour E, Gholipour M, Seyyed Nejad SM. PMC2897020
20607490 Five compounds, styraxjaponoside A (1), matairesinoside (2), egonol glucoside (3), dihydrodehydrodiconiferyl alcohol 9'-O-glucoside (4), and styraxjaponoside B (5) were isolated from the stem bark of Styrax japonica. Among them, compounds 1 and 5 showed significantly high virus-cell fusion inhibitory activity. In addition, compound 5 exhibited almost equivalent virus-cell fusion inhibitory activity to that of dextran sulfate, which is used as a positive control. Isolation of virus-cell fusion inhibitory components from the stem bark of Styrax japonica S. et Z. Lee DG, Jin Q, Jin HG, Shin JE, Choi EJ, Woo ER. No
20608716 Six new compounds, pruniflorones M-R (1-6), together with 19 known compounds (7-25) were isolated from the stems of Cratoxylum formosum ssp. pruniflorum. The structures of the new compounds were established on the basis of extensive spectroscopic data interpretation. In addition, their RXRalpha transcriptional activities were evaluated using an in vitro assay. Bioactive xanthones from the stems of Cratoxylum formosum ssp. pruniflorum. Duan YH, Dai Y, Wang GH, Zhang X, Chen HF, Chen JB, Yao XS, Zhang XK. No
20614816 A new flavone, vogeol, and a new isoflavone, vogliiol, have been isolated from Erythrina vogelii, a Cameroonian medicinal plant, along with seven known constituents (salvigenin, carpachromene, euchrenone b10, scandenone, 5,4'-dihydroxy-8-(3"-methylbut-2"-enyl)-2'''-(4'''-hydroxy-4'''-methylethyl)-furano-[4''',5''':6,7] isoflavone, erythrivarone C, and 5,7,4'-trihydroxy-6-[3"-methylbut-3"-enyl]-flavone). Their structures were elucidated by spectroscopic methods. Flavonoids from Erythrina vogelii (Fabaceae) of Cameroon. Ali MI1, Ahmed Z, Waffo AF, Ali MS. No
20659547 Hypericum perforatum L. (Hypericaceae) is a perennial herb that is commonly known as St. John's Wort. The plant has been valued for its important biological and chemical perspectives and its use in the treatment of infectious diseases has been documented in ethnobotanical reports. Most recent interest in H. perforatum has focused on its antidepressant effects, and only recently has its antimicrobial activity been evaluated against a number of bacterial and fungal strains. The present review gives a comprehensive summary of the ethnobotanical uses, chemical constituents and biological effects (antibacterial and antifungal) of this species. A comprehensive account of the chemical constituents including anthraquinone derivatives (naphthodianthrones), flavonoids, prenylated phloroglucinols, tannins and volatile oils is also included. Various types of preparations, ointments, creams and extracts prepared with and compounds isolated from this species have been found to possess a broad spectrum of biological and pharmacological effects such as antidepressant effects, wound-healing, antiviral and antimicrobial activity. The antibacterial activity of crude extracts can be related to the use of the herb as a wound healer in ancient times. The sole antibacterial principle isolated to date is a tetraketone, hyperforin, also thought to be responsible for the antidepressant activity of the herb. The available literature indicates that it has a higher antibacterial activity against Gram-positive than Gram-negative bacteria, and alcoholic extracts (methanolic/ethanolic) were shown to possess more pronounced activity than aqueous extracts. Based on the chemical and pharmacological characteristics of H. perforatum, we concluded that this species has beneficial therapeutic properties and has the potential for use as an effective adaptogenic herbal remedy. A review of the antibacterial activity of Hypericum perforatum L. Saddiqe Z, Naeem I, Maimoona A. No
20668579 Satureja Macrostema is used both as a functional food and as a drug. In this study, the antioxidative potential of the methanol extract of Satureja Macrostema (SM) was evaluated using various antioxidant assays, including DPPH, superoxide, nitric oxide (NO), hydroxyl radical scavenging and iron-chelating activity. Total phenolic and flavonoid content of SM was also determined by a colorimetric method. The extract exhibited powerful free radical scavenging, especially against DPPH, hydroxyl radical scavenging and iron-chelating activity as well as a moderate effect on NO and superoxide anions. The protective effects of methanol extract of SM were studied in carbon tetrachloride-reduced biochemical markers of hepatic injury such as glutamate pyruvate transaminase (SGPT), serum glutamate oxalaoacetate transaminase (SGOT), alkaline phosphatase (ALP), serum bilirubin, cholesterol alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. The increased level of HDL demonstrated dose dependant reduction in the in vivo peroxidation induced by CCl4. SM could protect from paracetamol-induced lipid peroxidation eliminating the deleterious effects of toxic metabolites from paracetamol. Degree of protection was measured by using biochemical parameters such as serum transaminase (GOT and GPT), alkaline phosphatase (ALKP) and bilirubin. Hexane and chloroform extracts did not show any effects. Results obtained in the present study suggest that S. Macrostema elicits hepatoprotectivity through antioxidant activity on carbon tetrachloride- and paracetamol-induced hepatic damage in rats. Antioxidant and hepatoprotective effects of the methanol extract of the leaves of Satureja macrostema. Gutierrez RM, Navarro YT. PMC2900060
20668877 The present study was carried out to establish the larvicidal activities of crude and solvent extracts of Solanum nigrum L. leaves against Culex quinquefasciatus Say as target species. The results indicated that the mortality rates at 0.5% concentration were highest amongst all concentrations of the crude extracts tested against all the larval instars at 24, 48 and 72 h of exposure. Result of log probit analysis (at 95% confidence level) revealed that lethal concentration LC(50) and LC(90) values gradually decreased with the exposure periods in bioassay experiment with the crude plant extract. The results of regression analysis of crude extract of S. nigrum revealed that the mortality rate is positively correlated with the concentration of the extracts. Mature leaves of S. nigrum were also extracted with six different solvents [viz. petroleum ether, benzene, ethyl acetate, chloroform: methanol (1:1 v/v), acetone and absolute alcohol] to determine the best extractant for subsequent isolation and characterization of active ingredient. Mortality rate with ethyl acetate extract was significantly higher (p < 0.05) than other extracts when 50-ppm doses were used. The corresponding LC(50) value of acetone, absolute alcohol, petroleum ether, chloroform: methanol (1:1 v/v), benzene and ethyl acetate extracts were 72.91 ppm, 59.81 ppm, 54.11 ppm, 32.69 ppm, 27.95 ppm and 17.04 ppm, respectively, after 24 h of exposure period. Results of this study show that the ethyl acetate extract of S. nigrum may be considered as a potent source of a mosquito larvicidal agent. Mosquito larvicidal activities of Solanum nigrum L. leaf extract against Culex quinquefasciatus Say. Rawani A, Ghosh A, Chandra G. No
20677781 Two new dimeric xanthone O-glycosides, puniceasides A (1) and B (2), a new trimeric O-glycoside, puniceaside C (3), and two new trimeric C-glycosides, puniceasides D (4) and E (5), together with 12 known xanthones were isolated from the entire plant of Swertia punicea. The structures of 1-5 were determined by HRESIMS and NMR spectroscopic methods. Compounds 2, 6, and 7 exhibited potent neuroprotective activity against H(2)O(2)-induced PC12 cell damage. Neuroprotective xanthone glycosides from Swertia punicea. Du XG, Wang W, Zhang SP, Pu XP, Zhang QY, Ye M, Zhao YY, Wang BR, Khan IA, Guo DA. No
20707195 OBJECTIVE: To study the alkaloids in the stems and leaves of Stephania cepharantha.METHOD: The dried stems and leaves of S. cepharantha were percolated with 95% ethanol and the solvent was removed by rotary evaporation to give a concentrate, and the concentrate was extracted by petroleum ether and chloroform. Column chromatograghy on MCI CHP 20P, silica gel, Rp-18, Sephadex LH-20 and polyamide were applied for the isolation and purification of the chloroform fraction. The structures were elucidated by their physicochemical properties and spectral data.RESULT: Eleven alkaloids were obtained and identified as lysicamine (1), tetrahadropalmatine (2), palmatine (3), isocorydione (4), corydalmine (5), corypalmine (6), sinoracutine (7), sinoacutine (8), cepharamine (9), isocorydine (10) and corydine (11).CONCLUSION: Compounds 2-7 were isolated from S. cepharantha for the first time, and compound 7 was isolated from the genus Stephania for the first time, compound 4 was isolated from the Menispermaceae family for the first time. [Alkaloids in stems and leaves of Stephania cepharantha]. He L, Zhang Y, Tang L, Song S, Sun Q. No
20730967 In our screening program for new agrochemicals from local wild plants, Artemisia lavandulaefolia and A. sieversiana were found to possess insecticidal activity against the maize weevil Sitophilus zeamais. The essential oils of the aerial parts of the two plants were obtained by hydrodistillation and analyzed by GC and GC/MS. The main components of A. lavandulaefolia oil were caryophyllene (15.5%), beta-thujone (13.8%), eucalyptol (13.1%), and beta-farnesene (12.3%), and the principal compounds identified in A. sieversiana oil were eucalyptol (9.2%), geranyl butyrate (9.2%), borneol (7.9%), and camphor (7.9%). The essential oils of A. lavandulaefolia and A. sieversiana possessed fumigant toxicity against S. zeamais adults with LC(50) values of 11.2 and 15.0 mg/l air, respectively. Both essential oils also showed contact toxicity against S. zeamais adults with LD(50) values of 55.2 and 112.7 microg/adult, respectively. Insecticidal activity and chemical composition of the essential oils of Artemisia lavandulaefolia and Artemisia sieversiana from China. Liu ZL, Liu QR, Chu SS, Jiang GH. No
20732375 The essential oil and methyl ester of hexane extract of Salvia chionantha Boiss. were analysed by GC and GC-MS. Totally, 54 components were detected in the essential oil and all of them were fully determined. Germacrene D (25.03%), β-caryophyllene (8.71%), spathulenol (5.86%) and α-humulene (4.82%) were identified as the major compounds. In the methylated hexane extract, 3-hydroxy hexadecanoic acid (39.39%), 3-hydroxy tetradecanoic acid (12.66%) and palmitic acid (12.02%) were the major fatty acids elucidated. The antioxidant activity of the essential oil and the hexane extract was determined by using four complementary test systems; namely, β-carotene-linoleic acid, DPPH() scavenging, ABTS(+)* scavenging, and CUPRAC assays. In β-carotene-linoleic acid assay, the extract showed 81.2±0.1% lipid peroxidation inhibition at 0.8 mg/mL concentration, while in ABTS(+)* assay the essential oil exhibited 77.4±0.5% inhibition at same concentration. Since, acetylcholinesterase and butyrylcholinesterase enzymes are taking place in pathogenesis of Alzheimer's disease, in vitro anticholinesterase activity of the essential oil and the extract was also studied spectrophotometrically. At 0.5mg/mL concentration, the essential oil showed moderate acetylcholinesterase (56.7±1.9%) and butyrylcholinesterase (41.7±2.9%) inhibitory activity, while the extract was only exhibited activity (63.1±0.8%) against butyrylcholinesterase enzyme. Hence, the essential oil may be useful as a moderate anticholinesterase agent, particularly against acetylcholinesterase. Chemical composition of the essential oil and hexane extract of Salvia chionantha and their antioxidant and anticholinesterase activities. Tel G, Oztürk M, Duru ME, Harmandar M, Topçu G. No
20736910 In our screening program for new agrochemicals from Chinese medicinal herbs, Murraya exotica was found to possess insecticidal activity against the maize weevil, Sitophilus zeamais and red flour beetle, Tribolium castaneum. The essential oil of aerial parts of M. exotica was obtained by hydrodistillation and investigated by GC and GC-MS. The main components of M. exotica essential oil were spathulenol (17.7%), alpha-pinene (13.3%), caryophyllene oxide (8.6%), and alpha-caryophyllene (7.3%). Essential oil of M. exotica possessed fumigant toxicity against S. zeamais and T. castaneum adults with LC(50) values of 8.29 and 6.84 mg/L, respectively. The essential oils also show contact toxicity against S. zeamais and T. castaneum adults with LD(50) values of 11.41 and 20.94 microg/adult, respectively. Chemical composition and toxicity against Sitophilus zeamais and Tribolium castaneum of the essential oil of Murraya exotica aerial parts. Li WQ, Jiang CH, Chu SS, Zuo MX, Liu ZL. No
20803382 A novel dimeric secoiridoid glucoside, gentimacroside (1), and six known compounds (2-7) were isolated from the roots of Gentiana macrophylla Pall. Their structures were established on the basis of spectroscopic analysis, especially by means of 1D-, 2D-NMR and HRESIMS analyses. Jiang ZB, Liu HL, Liu XQ, Shang JN, Zhao JR, Yuan CS. Chemical constituents of Gentiana macrophylla Pall. No
20806076 Abstarct:"PURPOSE:Advanced glycation end products (AGE) are associated in the development of several pathophysiologies including diabetic cataract. Earlier we have reported that some common dietary agents have antiglycating activity and ginger (Zingiber officinalis) was one of the few prominent agents that effectively prevented AGE formation in vitro. In this study we investigated the potential of ginger to prevent diabetic cataract in rats. Antiglycating potential of Zingiber officinalis and delay of diabetic cataract in rats. Saraswat M, Suryanarayana P, Reddy PY, Patil MA, Balakrishna N, Reddy GB. PMC2925903
20815211 The xanthones in the ethyl acetate extract of Comastoma pedunlulatum were investigated. The chromatographic and spectroscopic techniques were used to isolate and identify the constituents. Nine xanthones were isolated from the active parts of the ethyl acetate portion of the 70% ethanolic extract of C. Pedunlulatum, which possess the protective activity against hepatocyte damage caused by DL-GalN, and identified as 1,8-dihydroxy-2,6-dimethoxyxanthone (1), 8-hydroxy-1,2,6-trimethoxyxanthone (2), 1,6,8-trihydroxy-2-methoxyxanthone (3), 1,8-dihydroxy-3,5-dimethoxyxanthone (4), 1-hydroxy-3,5,8-trimethoxyxanthone (5), 1 -hydroxy-3,7-dimethoxyxanthone (6), 1,2,6,8-tetrahydroxyxanthone (7), 1,3,7-trihydroxy4- methoxyxanthone (8), 6,8-dihydroxy-1, 2-dimethoxyxanthone (9). Among them, compounds 6-9 were isolated from the genus Comastoma for the fist time. [Xanthones of Comastoma pedunlulatum]. Yuan Y, Cui B, Zhang Y, Li S. No
20818927 Abstarct:"OBJECTIVE:The study evaluates, qualitatively and quantitatively, the volatile oil profiles of the aerial parts of Jordanian garland Chrysanthemum coronarium L. (Asteraceae) and compares the findings with literature reports of garland of other sources, in terms of general composition and content of potentially active components.MATERIALS AND METHODS:The chemical composition of the essential oils isolated by hydrodistillation from dried material composed of flowerheads (FH) and aerial parts except for flowerheads (AEF) was assessed by GC-FID and GC-MS.RESULTS AND DISCUSSION:More than 60 components were identified in the studied oils, corresponding to about 99.6 and 99.7% of total oil constituents of FH and AEF, respectively. The oil was characterized by substantial levels of monoterpenes (76.9% in FH and 61.9% in AEF) and moderate levels of sesquiterpenes (15.7% in FH and 27.7% in AEF). The oil from FH was characterized by high levels of oxygenated monoterpenes (64.3%, compared to 15.3% in AEF) and moderate levels of both monoterpene-hydrocarbons (12.6%, compared to 46.6% in AEF as the major fraction) and sesquiterpene-hydrocarbons (14.7%, compared to 23.5% in AEF), while very low levels of oxygenated sesquiterpenes were observed in both oils (1.0% in FH and 4.2% in AEF). The principal oil component was camphor (17.5%) in FH and myrcene (36.7%) in AEF. Other major components identified in the FH oil were santolina triene (4.3%), neoiso-3-thujanol (5.6%), cis-chrysanthenyl acetate (10.8%), perilla aldehyde (11.7%), iso-italicene (4.7%), phenylpropyl butanoate (4.9%), and germacrene D (4.3%), while Z-β-ocimene (5.2%), isobornyl acetate (5.2%), E-β-farnesene (12.1%), and germacrene D (4.5%) were the major constituents of AEF oil." Volatile oil profiles of the aerial parts of Jordanian garland, Chrysanthemum coronarium. Tawaha K, Hudaib M. No
20839620 Two geranylated and methylated flavonol derivatives, macarhizinoidins A (1) and B (2), along with a known phenolic compound methyl 4-isoprenyloxycinnamate (3), have been isolated from the methanol extract of the leaves M. rhizinoides. The structures of these compounds were identified based on their spectroscopic data. On cytotoxic evaluation against murine leukemia P-388 cells, compounds 1-2 showed IC50 values of 11.4 and 13.9 microM, respectively, while compound 3 was inactive. Geranylated flavonols from Macaranga rhizinoides. Tanjung M, Mujahidin D, Hakim EH, Darmawan A, Syah YM. No
20839636 The essential oil from the leaves of Tagetes pusilla Kunth (Asteraceae) collected from Mérida, Venezuela, was analyzed by GC/MS. A yield of 0.38% oil was obtained by hydrodistillation. Only two components, trans-anethole and 4-allylanisole were identified by comparison of their mass spectra with those in the Wiley GC-MS Library data base. Volatile compounds from Tagetes pusilla (Asteraceae) collected from the Venezuela Andes. Buitrago D, Rojas LB, Rojas J, Morales A. No
20845784 Abstarct:"OBJECTIVE:To study the chemical constituents from Solanum nigrum.METHODS:Compounds were isolated and purified by silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified by physicochemical properties and spectral analysis.RESULTS:Six compounds were isolated and identified as (+)-pinoresinol (I), (+)-syringaresinol (II), (+)-medioresinol (III), scopoletin (IV), tetracosanoic acid (V) and beta-sitosterol (VI).CONCLUSION:Compounds I - III are isolated from this genus for the first time, while compounds IV and V are isolated from this plant for the first time." [Studies on the chemical constituents of Solanum nigrum]. Zhao Y, Liu F, Lou HX. No
20890571 Abstarct:"INTRODUCTION: Fractioning of an extract of Valeriana pavonii, a native species used in Colombian folk medicine as tranquilizer, led to the isolation and identification of isovaleramide, one of the active constituents responsible for its central nervous system activity as anticonvulsant.OBJECTIVE: Description of the isolation and identification of isovaleramide, an active principle on central nervous system from Valeriana pavonii.MATERIALS AND METHODS: The purification of isovaleramide was carried out by chromatographic techniques. Its structural elucidation was determined by nuclear magnetic resonance and mass spectrometry. Maximal electroshock seizure was used as in vivo pharmacological test, additionally in vitro GABA-A/BDZ-binding site studies were performed.RESULTS: Isovaleramide was isolated from the most active fraction of Valeriana pavonii. This compound, at 100 mg/Kg, p.o, evidenced a 90% index protection against the maximal electroshock seizure in mice (MES), comparable to the reference agent: sodium phenytoin (20 mg/kg, p.o, 100%). In the in vitro assay, isovaleramide (300 µM) exhibited a 42% of inhibition of the binding of ³H-FNZ to its sites.CONCLUSION: Isovaleramide is one of the active anticonvulsant constituents of Valeriana pavonii, for the first time reported in this species. These results support the traditional use of Valeriana pavonii and its interest as a therapeutic source." Isovaleramide, an anticonvulsant molecule isolated from Valeriana pavonii Giraldo SE, Rincón J, Puebla P, Marder M, Wasowski C, Vergel N, Guerrero MF No
20927694 Bioassay-guided fractionation of the fruit pericarp of Pentadesma butyracea, using the antiplasmodial test, led to the isolation of a new xanthone, named pentadexanthone (1), together with six known compounds: cratoxylone (2), α-mangostin (3), 1,3,5-trihydroxy-2-methoxyxanthone (4), garcinone E (5), (-)-epicathechin (6), and lupeol (7). The structure of 1 was elucidated by spectroscopic data analysis. An antiplasmodial assay was performed with the isolates, in which compounds 1- 3 and 5 exhibited potent activity in vitro against Plasmodium falciparum chloroquine-resistant strain W2, with IC₅₀ values below 3 µM. Antiplasmodial constituents from the fruit pericarp of Pentadesma butyracea. Lenta BN, Kamdem LM, Ngouela S, Tantangmo F, Devkota KP, Boyom FF, Rosenthal PJ, Tsamo E. No
20929258 Psiguadials A (1) and B (2), two novel sesquiterpenoid-diphenylmethane meroterpenoids with unusual skeletons, along with a pair of known epimers, psidial A (3) and guajadial (4), were isolated from the leaves of Psidium guajava. Their structures with absolute configurations were elucidated by means of NMR, X-ray diffraction, and quantum chemical CD calculation. Compounds 1, 2, and 4 exhibited potent inhibitory effects on the growth of human hepatoma cells. Psiguadials A and B, two novel meroterpenoids with unusual skeletons from the leaves of Psidium guajava. Shao M, Wang Y, Liu Z, Zhang DM, Cao HH, Jiang RW, Fan CL, Zhang XQ, Chen HR, Yao XS, Ye WC. No
20948498 Bioassay-guided fractionation of the ethyl acetate bark extract of Rheedia acuminata led to the isolation of the new compound 1,5,6-trihydroxy-3-methoxy-7-geranyl-xanthone, together with four known compounds. These compounds were tested in vitro for their antiplasmodial activity on a chloroquine-resistant strain of Plasmodium falciparum (FcB1) and for their cytotoxicity against the human diploid embryonic lung cell line MRC-5. A new xanthone from the bark extract of Rheedia acuminata and antiplasmodial activity of its major compounds. Marti G, Eparvier V, Litaudon M, Grellier P, Guéritte F. No
20954093 A new prenylated xanthone, named bannaxanthone I, has been isolated from the leaves of Garcinia xipshuanbannaensis, along with five other known compounds, bannaxanthone E, mangostinone, tovophyllin A, garcinone E, and γ-mangostin. The structure of the new compound was elucidated on the basis of high-resolution fast atom bombardment mass spectra (HRFABMS), 1D-and 2D-NMR experiments, including heteronuclear multiple bond connectivity (HMBC), heteronuclear single quantum coherence (HSQC) and ¹H-¹H-COSY (correlated spectroscopy, COSY). A new prenylated xanthone from Garcinia xipshuanbannaensis Y.H. Li. Na Z, Xu YK. No
21043475 Two new norlignans, hyperiones A (1) and B (2), three new acylphloroglucinols, aspidinol C (3) and hyperaspidinols A (5) and B (6), the known compound aspidinol D (4), and the symmetrical dimeric xanthone hyperidixanthone (7) were isolated from Hypericum chinense. Their structures were established by spectroscopic analysis. In an antibacterial assay using a panel of multidrug-resistant (MDR) strains, compounds 3 and 4 exhibited promising activity against the NorA efflux protein overexpressing MDR Staphylococcus aureus strain SA-1199B with a minimum inhibitory concentration (MIC) of 2 μg/mL (8.4 μM) and 4 μg/mL (16.8 μM), respectively. The positive control antibiotic norfloxacin showed activity at MIC 32 μg/mL (100 μM). Norlignans, acylphloroglucinols, and a dimeric xanthone from Hypericum chinense. Wang W, Zeng YH, Osman K, Shinde K, Rahman M, Gibbons S, Mu Q. No
21048341 A new geranylated coumarin, (E)-4-(1-hydroxypropyl)-5,7-dihydroxy-6-(3,7-dimethyl-2,6-octadienyl)-8-(3-methyl-1-oxobutyl)coumarin (named surangin D), was isolated from the bark of Mammea siamensis collected in Vietnam, along with four known coumarins, surangins B and C, and theraphins B and C, and seven xanthones, 1,7-dihydroxyxanthone, 7-hydroxy-1-methoxyxanthone, 1,7-dimethoxyxanthone, 1,7-dimethoxy-6-hydroxyxanthone, 1,6,7-trihydroxyxanthone, 1,3,7-trihydroxyxanthone, and 1,7-dihydroxy-3-methoxyxanthone. Their structures were determined by spectroscopic methods (mainly 1D- and 2D-NMR) and preparation of methylated derivatives. The four coumarins, surangins C and D and theraphins B and C, were tested for inhibition of cell proliferation in DLD-1 (colon cancer), MCF-7 (breast adenocarcinoma), HeLa (human cervical cancer) and NCI-H460 (human lung cancer) cell lines using the sulforhodamine B (SRB) assay. In all four cell lines, theraphin C showed the strongest activity (IC₅₀ in the range of 1.6-5.7 µM). Testing the anti-proliferative effect of the methylated derivatives showed reduced cellular effects of all derivatives, indicating that the number and position of free hydroxyl groups were very important for the anti-proliferative effect. Cytotoxic Coumarins from the Bark of Mammea siamensis. Ngo NT, Nguyen VT, Vo HV, Vang O, Duus F, Ho TD, Pham HD, Nguyen LH. No
21055781 From the 1-BuOH-soluble fraction of a MeOH extract of leaves of Croton cascarilloides, collected in Okinawa, Japan, seven megastigmane glycosides, named crotonionosides A-G, were isolated together with three known megastigmane glucosides, dendranthemosides A and B, and citroside A. This structures were elucidated by a combination of spectroscopic analyses, HPLC analyses, and application of the modified Mosher's method. Crotonionosides A-G: megastigmane glycosides from leaves of Croton cascarilloides Räuschel. Kawakami S1, Matsunami K, Otsuka H, Shinzato T, Takeda Y. No
21105198 Abstarct:"AIM:To evaluate the anti-Helicobacter pylori (H. pylori) activity of 50 traditional Chinese herbal medicines in order to provide the primary evidence for their use in clinical practice.METHODS:A susceptibility test of water extract from 50 selected traditional Chinese herbal medicines for in vitro H. pylori Sydney strain 1 was performed with broth dilution method. Anti-H. pylori activity of the selected Chinese herbal medicines was evaluated according to their minimum inhibitory concentration (MIC).RESULTS:The water extract from Rhizoma Coptidis, Radix Scutellariae and Radix isatidis could significantly inhibit the H. pylori activity with their MIC less than 7.8 mg/mL, suggesting that traditional Chinese herbal medicines have anti-inflammatory and antibacterial effects and can thus be used in treatment of H. pylori infection.CONCLUSION:Rhizoma Coptidis, Radix Scutellariae and Radix isatidis are the potential sources for the synthesis of new drugs against H. pylori." Screening test for anti-Helicobacter pylori activity of traditional Chinese herbal medicines. Ma F, Chen Y, Li J, Qing HP, Wang JD, Zhang YL, Long BG, Bai Y. PMC2992683
21128147 A new triterpenoid saponin, clematomandshurica saponin E, together with four known saponins were isolated and characterized from the roots and rhizomes of Clematis mandshurica (Ranunculaceae), a commonly used traditional Chinese medicine with anti-inflammatory and antirheumatoid activities. On the basis of spectroscopic analysis, including HR-ESI-MS, IR, 1D, and 2D NMR spectral data and hydrolysis followed by chromatographic analysis, the structure of the new triterpenoid saponin was elucidated as 3-O-α-L-rhamnopyranosyl-(1 → 6)-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 4)-β-D-ribopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl oleanolic acid 28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside. Clematomandshurica saponin E, a new triterpenoid saponin from Clematis mandshurica. Dong FY, Cui GH, Zhang YH, Zhu RN, Wu XJ, Sun TT, Wang W. No
21213980 Phytochemical investigation of Nepeta distans Raul resulted in the isolation of a new phenolic compound, nepatanol (1), and eight known compounds, markhamioside F, netidiol, nepedinol, thymoquinone, eugenol, oleanolic acid, ursolic acid, and beta-sitosterol, which have been isolated for the first time from this source. Structures of all the isolates were established on the basis of MS, and 1D and 2D NMR spectral data and by comparison with reported data. Chemical constituents of Nepeta distans. Hussain J, Bukhari N, Hussain H, Rehman NU, Hussain SM. No
21214233 Four new hasubanan-type alkaloids, cepharatines A-D (1-4), were isolated from the leaves and stems of Stephania cepharantha, and their structures were elucidated by spectroscopic analysis. The structure of 1 was further confirmed by X-ray crystallographic diffraction. Cepharatines A-D, hasubanan-type alkaloids from Stephania cepharantha. He L, Zhang YH, Guan HY, Zhang JX, Sun QY, Hao XJ. No
21247464 Abstarct:"BACKGROUND:Hepatitis C is a major health problem causes liver cirrhosis, hepatocellular carcinoma and death. The current treatment of standard interferon in combination with ribavirin, has limited benefits due to emergence of resistant mutations during long-term treatment, adverse side effects and high cost. Hence, there is a need for the development of more effective, less toxic antiviral agents.RESULTS:The present study was designed to search anti-HCV plants from different areas of Pakistan. Ten medicinal plants were collected and tested for anti-HCV activity by infecting the liver cells with HCV 3a innoculum. Methanol and chloroform extracts of Solanum nigrum (SN) seeds exhibited 37% and more than 50% inhibition of HCV respectively at non toxic concentration. Moreover, antiviral effect of SN seeds extract was also analyzed against HCV NS3 protease by transfecting HCV NS3 protease plasmid into liver cells. The results demonstrated that chloroform extract of SN decreased the expression or function of HCV NS3 protease in a dose- dependent manner and GAPDH remained constant.CONCLUSION:These results suggest that SN extract contains potential antiviral agents against HCV and combination of SN extract with interferon will be better option to treat chronic HCV." In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus. Javed T, Ashfaq UA, Riaz S, Rehman S, Riazuddin S PMC3032714
21288335 Abstarct:"BACKGROUND:The increasing number of multidrug-resistant Plasmodium strains warrants exploration of new anti-malarials. Medicinal plant research has become more important, particularly after the development of Chinese anti-malarial drug artemisnin from Artemisia annua. The present study shows evaluation of anti-malarial effects of two plants commonly used against malaria in the Garhwal region of north-west Himalaya, in order to discover the herbal-based medicine.METHODS:In vitro anti-plasmodial sensitivity of plant extracts was assessed using schizont maturation and parasite lactate dehydrogenase (pLDH) assay. Cytotoxic activities of the examined extracts were determined on L-6 cells of rat skeletal muscle myoblast. The 4-day test for anti-malarial activity against a chloroquine sensitive Plasmodium berghei NK65 strain in Swiss albino mice was used for monitoring in vivo activity of plant extracts.RESULTS:Chloroform extract of H. antidysenterica (HA-2) and petroleum ether extract of V. canescens (VC-1) plants significantly reduced parasitaemia in P. berghei infected mice. The extract HA-2 showed in vitro anti-plasmodial activity with its IC50 value 5.5 μg/ml using pLDH assay and ED50 value 18.29 mg/kg in P. berghei infected Swiss albino mice. Similarly petroleum ether extract of V. canescens (VC-1) showed in vitro anti-plasmodial activity with its IC50 value 2.76 μg/ml using pLDH assay and ED50 15.8 mg/kg in P. berghei infected mice. The extracts coded as HA-2 at 30 mg/kg and VC-1 at 20 mg/kg exhibited parasite inhibition in mice: 73.2% and 63.0% respectively. Of these two plant extracts, petroleum ether extract of V. canescens was found slightly cytotoxic.CONCLUSION:The present investigation reflects the use of these traditional medicinal plants against malaria and these plants may work as potential source in the development of variety of herbal formulations for the treatment of malaria." Anti-malarial activity of Holarrhena antidysenterica and Viola canescens, plants traditionally used against malaria in the Garhwal region of north-west Himalaya. Verma G, Dua VK, Agarwal DD, Atul PK. PMC3042422
21297304 Two new xanthone glycosides, namely pruniflorosides A and B (1, 2), a new benzophenone glycoside, prunifloroside C (3), and a new xanthone, pruniflorone S (4) were isolated from the stems of Cratoxylum formosum ssp. pruniflorum, along with six known xanthones (5-10). Their structures were determined on the basis of extensive spectroscopic analysis. In addition, their retinoid X receptor α (RXRα) transcriptional activities were evaluated in vitro. Xanthone and benzophenone glycosides from the stems of Cratoxylum formosum ssp. pruniflorum. Duan YH, Dai Y, Wang GH, Chen HF, Gao H, Chen JB, Yao XS, Zhang XK. No
21299135 The chemical compositions of the essential oils from the peel of ripe and unripe fruits of Hymenaea courbaril L., obtained by hydrodistillation, were analyzed by GC and GC-MS. The main constituents of the essential oil from the peel of the ripe fruits were the sesquiterpenes alpha-copaene (11.1%), spathulenol (10.1%) and beta-selinene (8.2%), while germacrene-D (31.9%), beta-caryophyllene (27.1%) and bicyclogermacrene (6.5%) were the major compounds in the oil from unripe fruits. The essential oils were tested against Aedes aegypti larvae and showed LC50 values of 14.8 +/- 0.4 microg/mL and 28.4 +/- 0.3 microg/mL for the ripe and unripe fruit peel oils, respectively. From the peel of the ripe fruits, the diterpenes zanzibaric acid and isoozic acid were isolated, along with the sesquiterpene caryolane-1,9beta-diol. To the best of our knowledge, this is the first report of this sesquiterpene in the genus. The structures of all compounds isolated were identified on the basis of their spectral data (IR, MS, 1D- and 2D-NMR) and by comparison with literature spectral data. Chemical constituents and larvicidal activity of Hymenaea courbaril fruit peel. Aguiar JC, Santiago GM, Lavor PL, Veras HN, Ferreira YS, Lima MA, Arriaga AM, Lemos TL, Lima JQ, de Jesus HC, Alves PB, Braz-Filho R. No
21304613 Bulb extracts of Gladiolus dalenii reportedly used in the treatment of fungal infections in HIV/AIDS patients in the Lake Victoria region were tested for antifungal activity using the disc diffusion assay technique. Commercially used antifungal drugs, Ketaconazole and Griseofulvin (Cosmos Pharmaceuticals) were used as standards. Dichloromethane (CH₂CL₂)/Methanol (MeOH) in the ratio 1:1. Soluble extracts showed antifungal activity against Aspergillus niger. Direct bioautography on silica gel Thin Layer Chromatography (TLC) and appropriate spraying agents were used to identify the active component in the extract. The activities of both the extracts were higher than that of Griseofulvin. CH₂CL₂ soluble extract in addition showed ability to delay sporulation in A.niger. The active group of compounds in the extracts was identified as alkaloids, which offer immense potential for development of new and valuable pharmaceutical products. Antifungal Activity of Crude Extracts of Gladiolus Dalenii van Geel (Iridaceae) Odhiambo JA, Siboe GM, Lukhoba CW, Dossaji SF. PMC3005389
21306653 Abstarct:"BACKGROUND:Many medicinal plants from Leguminosae family can be found easily in Malaysia. These plants have been used as traditional medicines by local ethnic groups, where they are prepared as decoction, pastes for wound infections, and some have been eaten as salad. This paper focused on the assessment of antioxidant potential, antibacterial activity and classes of phytochemicals of nine plants from the Leguminosae family. Assessment of phytochemical content, polyphenolic composition, antioxidant and antibacterial activities of Leguminosae medicinal plants in Peninsular Malaysia Chew YL, Chan EW, Tan PL, Lim YY, Stanslas J, Goh JK. No
PMC3045388
21338993 An acylphloroglucinol, elliptophenone A, and two xanthones, elliptoxanthone A and elliptoxanthone B, were isolated from the aerial portions of Hypericum ellipticum together with three known xanthones, 1,3,7-trihydroxy-8-(3-methyl-2-butenyl)-9H-xanthen-9-one, 1,6-dihydroxy-4-methoxy-9H-xanthen-9-one, and 1,4,5-trihydroxy-9H-xanthen-9-one. Their structures were determined by spectroscopic analyses. The acylphloroglucinol and xanthones were evaluated for cytotoxicity using three human colon cancer cell lines cell lines (HT-29, HCT-116 and Caco-2) and a normal human colon cell line (CCD-18Co). Acylphloroglucinol and xanthones from Hypericum ellipticum. Manning K, Petrunak E, Lebo M, González-Sarrías A, Seeram NP, Henry GE. No
21348423 To investigate the chemical constituents of Psidium Guajava L, the EtOH/H2O extract of the fresh leaves was subjected to various chromatography. Five constituents with galloyl moiety were isolated and elucidated as 1-O-(1, 2-propanediol)-6-O-galloyl-beta-D-glucopyranoside (1), gallic acid (2), ellagic acid (3), ellagic acid-4-O-beta-D-glucopyranoside (4) and quercetin-3-O-(6"-galloyl) beta-D-galactopyranoside (5) by spectroscopic methods, including 2D NMR and HR-ESI-MS spectrometry as well as by comparison with published data. Compounds 4 and 5 were obtained from P. guajava for the first time, and compound 1 is a new polyhydroxyl compound. One new galloyl glycoside from fresh leaves of Psidium guajava L. Shu JC, Chou GX, Wang ZT. No
21348450 Three novel triterpenoids, henrischinins A-C (1-3), featuring the unique motif of a 3-one-2-oxabicyclo[3.2.1]-octane, were isolated from the leaves and stems of Schisandra henryi. Their structures were elucidated by spectroscopic methods, and the absolute configuration of 2 was confirmed by a single crystal X-ray diffraction. Compounds 1 and 2 showed weak cytotoxicity against HL-60 cell lines. Henrischinins A-C: three new triterpenoids from Schisandra henryi. Xue YB, Yang JH, Li XN, Du X, Pu JX, Xiao WL, Su J, Zhao W, Li Y, Sun HD. No
21351474 To study the chemical constituents of the fruits of Psoralea corylifolia L., the constituents were isolated and purified by chromatography. Two new compounds were isolated from the ethyl acetate-soluble fraction of the ethanol extract. On the basis of chemical and spectroscopic data 1D and 2D NMR, they were identified as 2-(3, 4-dihydroxyphenyl)-8, 8-dimethyl-8H-pyrano[2, 3-h]chromen-4-one (1) and 5-(2-hydroxyl-1-methyl)-ethyl-4, 5-dihydrofurano[2, 3-h](4'-hydroxyl-3', 5'-dimethoxy)-isoflavone (2), and named as coryfolia D and bavarigenin separately. [Two new compounds from Psoralea corylifolia L]. Yang TT, Li J, Qin MJ, Zhao M, Ouyang Z, Fu HZ. No
21354229 Abstarct:"BACKGROUND & AIMS:Hepatitis C virus (HCV) infection affects 3% of the world population and is the leading cause of chronic liver disease worldwide. Current standard of care is effective in only 50% of the patients, poorly tolerated, and associated with significant side effects and viral resistance. Recently, our group and others demonstrated that the HCV lifecycle is critically dependent on host lipid metabolism and that its production is metabolically modulated.METHODS:The JFH1/Huh7.5.1 full lifecycle model of HCV was used to study the antiviral effects of naringenin on viral replication, assembly, and production. Activation of PPARα was elucidated using GAL4-PPARα fusion reporters, PPRE reporters, qRT-PCR, and metabolic studies. Metabolic results were confirmed in primary human hepatocytes.RESULTS:We demonstrate that the grapefruit flavonoid naringenin dose-dependently inhibits HCV production without affecting intracellular levels of the viral RNA or protein. We show that naringenin blocks the assembly of intracellular infectious viral particles, upstream of viral egress. This antiviral effect is mediated in part by the activation of PPARα, leading to a decrease in VLDL production without causing hepatic lipid accumulation in Huh7.5.1 cells and primary human hepatocytes. Long-term treatment with naringenin leads to a rapid 1.4 log reduction in HCV, similar to 1000U of interferon. During the washout period, HCV levels returned to normal, consistent with our proposed mechanism of action.CONCLUSIONS:The data demonstrates that naringenin is a non-toxic assembly inhibitor of HCV and that other PPARα agonists play a similar role in blocking viral production. The combination of naringenin with STAT-C agents could potentially bring a rapid reduction in HCV levels during the early treatment phase, an outcome associated with sustained virological response." Naringenin inhibits the assembly and long-term production of infectious hepatitis C virus particles through a PPAR-mediated mechanism. Goldwasser J, Cohen PY, Lin W, Kitsberg D, Balaguer P, Polyak SJ, Chung RT, Yarmush ML, Nahmias Y. PMC3197749
21355192 OBJECTIVE: To study the alkaloids in the stems and leaves of Stephania cepharantha Hayata.METHODS: The dried stems and leaves of Stephania cepharantha Hayata were percolated with 95% ethanol and the solvent was removed by rotary evaporation to give a concentrate, and the concentrate was extracted by petroleum ether and chloroform. Column chromatograghy on MCI CHP 20P, silica gel, Rp-18, Sephadex LH-20 and polyamide were applied for the isolation and purification of the chloroform fraction. The structures were elucidated by their physicochemical properties and spectral data.RESULTS: Five alkaloids were obtained and identified as, Stephasunoline (I) Aknadinine (II), Discretamine (III), Acutumine (IV), Sinomenine (V).CONCLUSION: Compounds I, III, IV are isolated from this plant for the first time, and compound IV is isolated from the genus for the first time. [Study on the alkaloids in the stems and leaves of Stephania cepharantha (II)]. He L, Zhang YH, Tang LJ, Song SH, Sun QY. No
21372415 Two novel alkaloids, saprosmine A (1) and saprosmine B (2), were isolated from the stem of Saprosma hainanense MERR., along with five known alkaloids: marcanine A (3); cleistopholine (4); 4-methoxycarbonyl-5,10-benzogquinolinequinone (5); liriodenine (6); and quinoline (7). The chemical structures were established on the basis of extensive spectroscopic (IR, 1D-NMR, 2D-NMR, MS) data analysis and by comparison with spectroscopic data reported in the literature. Compounds 1 to 6 were evaluated for in vitro cytotoxic activities against the SPC-A-1 (human lung cancer), BEL-7402 (human hepatocellular carcinoma), SGC-7901 (human gastric cancer), and K-562 (human myelogenous leukaemia) cancer cell lines. Compounds 1 and 2 exhibited weak cytotoxic activities against K-562 cells. Compounds 3 and 5 showed cytotoxic activities against all four cancer cell lines. Two novel alkaloids from the stem of Saprosma hainanense and their cytotoxic activities in vitro. Wang L, Chen GY, Han CR, Yuan Y, Yang B, Zhang Y, Wang J, Zhong XQ, Huang X. No
21409690 The investigation of chemical constituents from the branches of Calophyllum inophyllum Linn led to the isolation of a new prenylated xanthone, named caloxanthone Q (1), together with three known compounds, 2-deprenylrheediaxanthone B (2), jacareubin (3), and 6-deoxyjacareubin (4). Their structures were completely elucidated on the basis of spectroscopic methods (UV, IR, HR-ESI-MS, 1D NMR, and 2D NMR). A new prenylated xanthone from the branches of Calophyllum inophyllum. Wei DJ, Mei WL, Zhong HM, Zeng YB, Wu XD, Dai HF. No
21414372 The chemical composition of the essential oil from Gnaphlium affine was determined, and its antimicrobial and antioxidant activities were evaluated. Twenty-four compounds, representing 94.95% of the amount of total oil, were identified by gas chromatography-mass spectrometry (GC-MS) analysis. Main constituents of the essential oil were found to be eugenol (18.24%), linalool (10.62%), trans-caryophyllene (8.86%), α-terpineol (5.97%), p-cymene (5.75%), hexadecanoic acid (5.63%), γ-cadinene (4.98%), δ-cadinene (4.22%), α-humulene (3.22%), and (-)-β-elemene (3.15%). The essential oil revealed a remarkable antimicrobial effect against the tested food-borne microorganisms with the MIC and MBC values in the ranges of 0.2-1.56 μg/ml and 0.39-3.13 μg/ml, respectively. The essential oil showed a potent antioxidant activity in ABTS radical scavenging, lipid peroxidation and reducing power assay. It was suggested that the essential oil from G. affine may be a new potential source as natural antimicrobial and antioxidant agents applied in food systems. Chemical composition, antimicrobial and antioxidant activities of essential oil from Gnaphlium affine. Zeng WC, Zhu RX, Jia LR, Gao H, Zheng Y, Sun Q. No
21425684 The essential oils yield and composition of the aerial parts of A. annua var. CIM-Arogya grown in Uttarakhand, India were analyzed and compared by capillary GC and GC-MS at different stages of development. The analysis led to the identification of 81 constituents forming 91.0%-97.1% of the essential oils compositions. The essential oil content of the aerial parts was found to vary from 0.3% to 0.7% at different stages of growth. A. annua crop harvested at full flowering and seed setting stage gave higher yield of essential oil (0.6%, 0.7%) than that harvested at pre flowering (0.5%), late vegetative (0.4%, 0.5%), mid vegetative (0.4%, 0.4%) and early vegetative stages (0.3%, 0.3%). The essential oils at different stages of growth showed monoterpenoids (38.5%-72.0%) and sesquiterpenoids (22.2%-48.2%) as major grouped constituents. The major constituents identified were camphor (22.8%-42.6%), 1,8-cineole (3.7%-8.4%), linalool (<0.1%-11.9%), beta-caryophyllene (2.0%-9.2%), (E)-beta-farnesene (1.3%-8.5%), germacrene D (0.5%-7.3%) and 1-epi-cubenol (0.7%-5.2%) in essential oil samples collected at different crop stages. Variation in the volatile constituents of Artemisia annua var. CIM-Arogya during plant ontogeny. Padalia RC, Verma RS, Chauhan A, Chanotiya CS, Yadav A. No
21440018 Hepatitis C virus infection is a major health problem worldwide. Developing effective antiviral therapy for HCV is the need of the hour. The viral enzymes NS3 protease and NS5B RNA dependent RNA polymerase are essential enzymes for polyprotein processing and viral RNA replication and thus can be potential targets for screening anti-HCV compounds. A large number of phytochemicals are present in plants, which are found to be promising antiviral agents. In this study, we have screened inhibitory effect of different plant extracts against the NS3 and NS5B enzymes of hepatitis C virus. Methanolic extracts were prepared from various plant materials and their inhibitory effects on the viral enzymes were determined by in vitro enzyme assays. Effect on viral RNA replication was investigated by using TaqMan Real time RT-PCR. Interestingly, Phyllanthus amarus root (PAR) extract showed significant inhibition of HCV-NS3 protease enzyme; whereas P. amarus leaf (PAL) extract showed considerable inhibition of NS5B in the in vitro assays. Further, the PAR and PAL extracts significantly inhibited replication of HCV monocistronic replicon RNA and HCV H77S viral RNA in HCV cell culture system. However, both PAR and PAL extracts did not show cytotoxicity in Huh7 cells in the MTT assay. Furthermore, addition of PAR together with IFN-α showed additive effect in the inhibition of HCV RNA replication. Results suggest the possible molecular basis of the inhibitory activity of PA extract against HCV which would help in optimization and subsequent development of specific antiviral agent using P. amarus as potent natural source. Inhibition of hepatitis C virus replication by herbal extract: Phyllanthus amarus as potent natural source. Ravikumar YS, Ray U, Nandhitha M, Perween A, Raja Naika H, Khanna N, Das S. No
21467674 Eleven new phragmalin-type limonoids, swietenitins N-X (1-11), together with a known one, epoxyfebrinin B (12), were isolated from the twigs of Swietenia macrophylla. The structures of the new compounds were established on the basis of spectroscopic methods, and that of compound 1 was confirmed by single-crystal X-ray diffraction. The stereochemistry of epoxyfebrinin B (12) was fully assigned in this study. Phragmalin-type limonoid orthoesters from the twigs of Swietenia macrophylla. Lin BD, Zhang CR, Yang SP, Wu Y, Yue JM. No
21480510 Hydrolysis of isoligustroside (1) and isooleuropein (2), secoiridoid glucosides, in the presence of β-glucosidase provided 2-(4-hydroxyphenyl)methyl (2R,3S,4S)-3-formyl-3,4-dihydro-4-(2-methoxy-2-oxoethyl)-2-methyl-2H-pyran-5-carboxylate (3) and 2-(3,4-dihydroxyphenyl)methyl (2R,3S,4S)-3-formyl-3,4-dihydro-4-(2-methoxy-2-oxoethyl)-2-methyl-2H-pyran-5-carboxylate (4), respectively. The structures of 3 and 4 were elucidated on the basis of extensive spectral analyses, including 2D-NMR experiments. Compounds 3 and 4 were found to be new rearrangement products of the aglycones of 1 and 2. The cytotoxic activities of 3 and 4 were evaluated using a disease-oriented panel of 39 human cancer cell lines and showed moderate cytotoxic activity for 4, while 3 exhibited weaker activity compared to that of 4. Structure and cytotoxic activity of enzymatic hydrolysis products of secoiridoid glucosides, isoligustroside and isooleuropein. Kikuchi M1, Yaoita Y, Mano N, Kikuchi M. No
21501041 Abstarct:"CONTEXT:Development of resistance in human pathogens against conventional antibiotic necessitates searching indigenous medicinal plants having antibacterial property. Twenty-seven medicinal plants used actively in folklore, ayurvedic and traditional system of medicine were selected for the evaluation of their antimicrobial activity for this study. Eleven plants chosen from these 27 are used as spices in local cuisine.OBJECTIVE:Evaluation of the effectiveness of some medicinal plant extracts against clinical isolates.MATERIAL AND METHODS:Nonedible plant parts were extracted with methanol and evaporated in vacuo to obtain residue. Powdered edible parts were boiled three times and cooled in sterile distilled water for 2 min each and filtrate collected. The minimum inhibitory concentration (MIC) of plant extracts and filtrates/antibiotics was evaluated against clinical isolates by microbroth dilution method.RESULTS:Water extract of Syzygium aromaticum L. (Myrtaceae) buds, methanol extracts of Ficus carica L. (Moraceae) and Olea europaea L. (Oleaceae) leaves and Peganum harmala L. (Nitrariaceae) seeds had MIC ranges of 31.25-250 µg/ml. S. aromaticum inhibited growth of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Salmonella enterica serovar Typhi and Pseudomonas aeruginosa. F. carica and O. europaea inhibited growth of S. aureus, S. epidermidis, and S. pyogenes whereas P. harmala was effective against S. aureus, Acinetobacter calcoaceticus and Candida albicans. Ampicillin, velosef, sulfamethoxazole, tetracycline and ceftazidime, cefotaxime, cefepime, which are used as control, had MIC ≥ 50 and 1.5 µg/ml, respectively, for organisms sensitive to extracts.DISCUSSION AND CONCLUSION:Mono/multiextract from identified plants will provide an array of safe antimicrobial agents to control infections by drug-resistant bacteria." Antibacterial activity in spices and local medicinal plants against clinical isolates of Karachi, Pakistan. Ali NH, Faizi S, Kazmi SU. No
21527328 ETHNOPHARMACOLOGICAL RELEVANCE:

In a search for new plant-derived biologically active compounds against protozoan parasites, an ethnopharmacological study was carried out to evaluate extracts from selected 17 traditional medicinal plants which were used by healers from the Garhwal region of North West Himalaya for the treatment of protozoal infections and fever including malaria.
MATERIALS AND METHODS:

In vitro activity against erythrocytic stages of Plasmodium falciparum was determined using a modified [3H]-hypoxanthine incorporation assay with the chloroquine- and pyrimethamine-resistant K1 strain. Activity against Trypanosoma brucei rhodesiense was performed on the STIB 900 strain and activity against Trypanosoma cruzi on infected rat skeletal myoblasts (L6 cells) seeded in 96-well microtitre plates while amastigotes of Leishmania donovani strain MHOM/ET/67/L82 were used to assess activity against Leishmania donovani. Cytotoxicity assays were performed against rat skeletal myoblasts (L6-cells).
RESULTS AND CONCLUSIONS:

Extracts of Artemisia roxburghiana, Roylea cinerea, Leucas cephalotes, Nepeta hindostana and Viola canescens showed good antiplasmodial activity (IC50<5 μg/ml). The chloroform extract of Artemisia roxburghiana was the most active (IC50 value of 0.42 μg/ml) and the most selective (SI=78) extract for Plasmodium falciparum among all plants extracts examined. The chloroform extract of Leucas cephalotes and the petroleum ether extract of Viola canescens exhibited substantial activities against Leishmania donovani with IC50 values of 3.61 μg/ml (SI=8) and 0.40 μg/ml (SI=30), respectively. The petroleum ether extract of Viola canescens exhibited activity against Trypanosoma cruzi with an IC50 value of 1.86 μg/ml (SI=7). Methanol and water extracts from all plants under investigation were found inactive against all parasites tested. These results support investigation of components of traditional medicines as potential new antiprotozoal agents. On the other hand since herbalism has become the main stream throughout the world, investigation demonstrates that these non-polar plant extracts of six of the plants examined in this study could play an important role in herbal formulations for the treatment of vector borne protozoal diseases.
Antiprotozoal activities of traditional medicinal plants from the Garhwal region of North West Himalaya, India. Dua VK, Verma G, Agarwal DD, Kaiser M, Brun R. No
21534036 Two new dibenzocyclooctadiene lignans, rubrilignans A and B (1, 2), together with 17 known ones, were isolated from the fruits of Schisandra rubriflora. The structures of 1 and 2 were elucidated by spectroscopic methods including extensive 1D and 2D NMR techniques. Compounds 1 and 2 were also evaluated for their anti-HIV-1 activities and showed weak anti-HIV-1 activity with EC(50) values of 2.26 and 1.82 μg/ml, and therapeutic index values of 35.5 and 18.6, respectively. Dibenzocyclooctadiene lignans from the fruits of Schisandra rubriflora and their anti-HIV-1 activities. Mu HX, Li XS, Fan P, Yang GY, Pu JX, Sun HD, Hu QF, Xiao WL. No
21534043 A new compound, 3β,18,19β-trihydroxylupane, was isolated from Garcinia tetralata, along with five known compounds, garcinexanthone B, morolic acid acetate, toxyloxanthone A, 6,11-dihydroxy-2,2-dimethylpyrano[3,2-c]xanthen-7(2H)-one, and 1,4-dihydroxy-5,6-dimethoxy-xanthone. The structure of the new compound was established by extensive spectroscopic techniques. Triterpenes and xanthones from the stem bark of Garcinia tetralata. Guo YE, Wang LL, Li ZL, Niu SL, Liu XQ, Hua HM, Chen H, Chu J, Zhang TC. No
21548365 OBJECTIVE: To study the alkaloids in Stephania hernandifolia.METHODS: The dried herbs of S. hernandifolia were extracted with 95% ethanol. After removal of the solvent, the residue was first partitioned between acid water and petroleum ether, then the aqueous layer was basified and extracted with chloroform to obtain crude alkaloids. Column chromatography on silica gel, Rp-18, MCI CHP 20P, Sephadex LH-20 were applied for the isolation and purification of the crude alkaloids fraction. The structures were elucidated by their physicochemical properties and spectral data.RESULTS: Six alkaloids were obtained and identified as Cepharamine (I), l-tetrahadropalmatine (II), Plmatine (III), Stephamiersine (IV), Telitoxine (V), Daurioxoisoporphine D (VI).CONCLUSIONS: Compounds I - VI are isolated from this plant for the first time, and compounds V, VI are isolated from the plants of Stephania for the first time. [Study on the alkaloids in Stephania hernandifolia]. Tang LJ, Guan HY, Zhang YH, He L, Yang XS, Hao XJ. No
21579475 The title compound, C(31)H(38)O(10) [systematic name: (αR,4R,4aR,6aS,7R,8S,10R,11S)-methyl α,10-di-acet-oxy-4-(3-furyl)-4a,7,9,9-tetra-methyl-2,13-dioxo-1,4,4a,5,6,6a,7,8,9,10,11,12-dodeca-hydro-7,11-methano-2H-cyclo-octa-[f][2]benzo-pyran-8-acetate], was isolated from the seeds of Swietenia macrophylla. The mol-ecule contains four six-membered rings connected together in the shape of a bowl; one of the inner rings adopts a twisted chair conformation owing to the carbon-carbon double bond. The furyl substitutent is connected to an outer ring, and it points away from the bowl cavity. Swietenolide diacetate from the seeds of Swietenia macrophylla. Goh BH, Abdul Kadir H, Abdul Malek SN, Ng SW. PMC2979530
21588997 The mol-ecule of O-acetyl-swietenolide, C(29)H(36)O(9), isolated from the seeds of Swietenia macrophylla, features four six-membered rings connected together in the shape of a bowl; one of the inner rings adopts a twisted chair conformation owing to the C=C double bond. The furyl substitutent is connected to an outer ring, and it points away from the bowl cavity. The hy-droxy group is connected to a carbonyl O atom of an adjacent mol-ecule by an O-H⋯O hydrogen bond, generating a chain running along the b axis.
Goh BH, Abdul Kadir H, Abdul Malek SN, Ng SW.
21602779 Swietenia mahogani crude methanolic (SMCM) seed extract was investigated for the antifungal activity against Candida albicans which has not been evaluated previously. The antifungal activity was evaluated against C. albicans via disk diffusion, minimum inhibition concentration (MIC), scanning electron microscope (SEM), transmission electron microscope (TEM) and time killing profile. The MIC value of SMCM seed extract is 12.5 mg/ml. The SEM and TEM findings showed there is morphological changes and cytological destruction of C. albicans at the MIC value. Animal model was used to evaluate the in vivo antifungal activity of SMCM seed extract. The colony forming unit (CFU) were calculated per gram of kidney sample and per ml of blood sample respectively for control, curative and ketaconazole treated groups. There was significant reduction for the CFU/ml of blood and CFU/g of kidney. This indicated that the extract was observed to be effective against C. albicans in vitro and in vivo conditions. In vitro and in vivo anticandidal activity of Swietenia mahogani methanolic seed extract. Sahgal G, Ramanathan S, Sasidharan S, Mordi MN, Ismail S, Mansor SM No
21626787 To investigate the chemical constituents of the roots of Andrographis paniculata, 28 compounds were isolated and identified from the 80% ethanol extract. There are 20 flavonoids: 5, 5'-dihydroxy-7, 8, 2'-trimetroxyflavone (1), 5-hydroxy-7, 8, 2', 6'-tetramethoxyflavone (2), 5, 3'-dihydroxy-7, 8, 4'-trimethoxyflavone (3), 2'-hydroxy-5, 7, 8-trimethoxyflavone (4), 5-hydroxy-7, 8, 2', 3', 4'-pentamethoxyflavone (6), wightin (7), 5, 2', 6'-trihydroxy-7-methoxyflavone 2'-O-beta-D-glucopyranoside (8), 5, 7, 8, 2'-tetramethoxyflavone (10), 5-hydroxy-7, 8-dimethoxyflavanone (11), 5-hydroxy-7, 8-dimethoxyflavone (12), 5, 2'-dihydroxy-7, 8-dimethoxyflavone (13), 5-hydroxy-7, 8, 2', 5'-tetramethoxyflavone (14), 5-hydroxy-7, 8, 2', 3'-tetramethoxyflavone (15), 5-hydroxy-7, 8, 2'-trimethoxyflavone (16), 5, 4'-dihydroxy-7, 8, 2', 3'-tetramethoxyflavone (17), dihydroneobaicalein (18), andrographidine A (19), andrographidine B (20), andrographidine C (21) and 5, 2'-dihydroxy-7, 8-dimethoxyflavone 2'-O-beta-D-glucopyranoside (22); three diterpenoids: andrograpanin (23), neoandrographolide (24) and andrographolide (25); two phenylpropanoids: trans-cinnamic acid (26) and 4-hydroxy-2-methoxycinnamaldehyde (5); and oleanolic acid (9), beta-sitosterol (27) and beta-daucosterol (28). Compound 1 is a new flavone, compound 4 is a new natural product, compounds 2, 3 and 5 were isolated from the Androggraphis genus for the first time and compounds 6-9 were isolated from this plant for the first time. [Chemical constituents from roots of Andrographis paniculata]. Xu C, Wang ZT. No
21641973 Bioassay-directed separation of the ethyl acetate extract from the aerial parts of Saussurea involucrata (Kar. et Kir. ex Maxim) led to the isolation of three new sesquiterpene lactones sausinlactones A (1), B (2), C (3) and six known ones (4-9). The structures were established by spectroscopic data (UV, IR, HRESIMS, 1D and 2D NMR). CD technique was also employed to determine the absolute configurations of new compounds 1-3. The anti-inflammatory activities of compounds 1-9 and antitumor activities of new compounds 1-3 were tested. The results presented that compounds 5 and 6 were responsible for anti-inflammatory activities, and compounds 1 and 2 showed significant cytotoxic activities against A549 cells. Sesquiterpene lactones from Saussurea involucrata. Xiao W, Li X, Li N, Bolati M, Wang X, Jia X, Zhao Y. No
21656353 Phytochemical investigation of Verbascum thapsus led to the isolation and identification of one new iridoid compound named verbathasin A, along with ten known compounds. The structure and relative stereochemistry of verbathasin A were elucidated by analysis of spectroscopic data. All the isolates except 10-deoxyeucommiol and ajugol were tested for antiangiogenic and antiproliferative activities, and compounds luteolin and 3-O-fucopyranosylsaikogenin F showed promising antiproliferative activities, with an obvious effect of inducing apoptosis of A549 lung cancer cells. Isolation of chemical constituents from the aerial parts of Verbascum thapsus and their antiangiogenic and antiproliferative activities Zhao YL, Wang SF, Li Y, He QX, Liu KC, Yang YP, Li XL. No
21663482 This study evaluated, in vitro, the antimicrobial activity of the hexane extract (JCHE), methanol extract (JCME), and chloroform fraction (JCCF) of bark from Jacaranda cuspidifolia Mart. (Family Bignoniaceae), a Brazilian medicinal plant, traditionally used as anti-syphilis and anti-gonorrhea treatment. The antimicrobial activity was evaluated using the disc diffusion method followed by the determination of minimum inhibitory concentration (MIC) values. JCHE was not active against the bacteria evaluated. JCME presented antibacterial activity against Streptococcus pyogenes, Staphylococcus aureus, and Neisseria gonorrhoeae with MIC values of 16.3 mg/mL, 9.1 mg/mL, and 25.2 mg/mL, respectively. JCCF was active against Staphylococcus epidermidis, S. aureus, Proteus mirabilis, Serratia marcescens, S. pyogenes, Enterobacter aerogenes, and N. gonorrhoeae with MIC values of 18.3 mg/mL, 9.3 mg/mL, 6.3 mg/mL, 6.1 mg/mL, 9.2 mg/mL, 6.2 mg/mL, and 25.2 mg/mL, respectively. Phytochemical analysis of JCME and JCCF gave positive results for saponins, coumarins, flavonoids, tannins, quinones, alkaloids, triterpenes, and steroids. Verbascoside was isolated and identified as a major peak in JCME and JCCF high-performance liquid chromatography fingerprints and might contribute to the observed antimicrobial activity. Jacaranda cuspidifolia Mart. (Bignoniaceae) as an antibacterial agent. Arruda AL, Vieira CJ, Sousa DG, Oliveira RF, Castilho RO. No
21682873 Abstarct:"BACKGROUND:Malaria is a major public health threat in Africa, and traditional medicine continues to play a key role in its control especially in rural areas. A bioassay-guided fractionation was carried out in order to evaluate the anti-malarial potential and the safety of the methanol extract of the Hypericum lanceolatum stem bark.METHODS:The anti-plasmodial activity was assayed by the lactate dehydrogenase method (pLDH) against the multidrug-resistant W2mef laboratory strain, and a field isolate (SHF4) of Plasmodium falciparum. Cytotoxicity tests were carried out using the LLC-MK2 monkey kidney epithelial cells.RESULTS:Five compounds were isolated from the most active and least cytotoxic ethylacetate sub-extract: betulinic acid (HLT1), 2,2',5,6'-tetrahydroxybenzophenone (HLT2), 5-hydroxy-3-methoxyxanthone (HLT3), 3-hydroxy-5-methoxyxanthone (HLT4) and HLT0 (yet to be identified). Three of the tested compounds presented significant anti-plasmodial activities (with 50% inhibitory concentration, IC₅₀ < 5 μM), with 5-hydroxy-3-methoxyxanthone exerting the highest activity, followed by HLT0 and betulinic acid. All the compounds with significant anti-plasmodial activity were non-cytotoxic, except betulinic acid which showed a 50% cytotoxic concentration, CC₅₀ of 25 μg/mL.CONCLUSIONS:These findings justify the use of H. lanceolatum stem bark as anti-malarial by traditional healers of Western Cameroon, and could constitute a good basis for further studies towards development of new drug candidates or phytomedicines for malaria." Hypericum lanceolatum (Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark Zofou D, Kowa TK, Wabo HK, Ngemenya MN, Tane P, Titanji VP. PMC3131257
21687350 Abstarct:"AIM:This study was designed to examine the chemical composition and in vitro antimicrobial potential of methanolic extract of Psidium guajava Linn (Myrtaceae).MATERIALS AND METHODS:The inhibitory effect of methanolic extract of P. guajava was tested against three bacterial and two fungal strains by using the paper disc diffusion method.RESULTS:The methanolic extract exhibited antibacterial activity against E. coli with minimum inhibitory concentration, 0.78 μg/ml, minimum bactericidal concentration of 50 μg/ml, and appreciable antifungal activity with minimum inhibitory concentration of 12.5 μg/ml. Preliminary phytochemical analysis of methanolic extract revealed the presence of antimicrobial compounds such as flavonoids, steroids, and tannins, which may contribute for the antimicrobial action of P. guajava.CONCLUSION:The extract was found to be bacteriostatic and fungistatic in action." In vitro antimicrobial activity of methanolic leaf extract of Psidium guajava L. Dhiman A, Nanda A, Ahmad S, Narasimhan B. PMC3103916
21695005 Greater cardamom (Amomum subulatum Roxb. Zingiberaceae) commonly known as "Bari ilaichi" is a well known plant used in Ayurvedic and Unani medicine. It has been used for the treatment of various diseases and disorders like gastric ulcer. Therefore antimicrobial activity of petroleum ether, methanol and aqueous extracts from leaves and roots, essential oil and isolated vasicine from A. vasica were tested against various microorganisms. Antimicrobial activity was done by disc diffusion method. The zone of inhibition observed was compared with that of standard drugs, ciprofloxacin and fluconazole. Minimum inhibitory concentration was determined against microorganisms used in the study. The results of this study reveal that methanol extract of fruits of A. subulatum shows remarkable antimicrobial activity against Escherichia coli whereas in case of other microorganisms used it was found inferior to the standard drug used. Methanol extract of rind showed good antimicrobial activity against Staphylococcus aureus. It was found that the essential oil isolated was effective against majority of microorganisms used viz. Bacillus pumilus, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Saccharomyces cerevisiae. Antimicrobial activity of essential oil and various extracts of fruits of greater cardamom. Agnihotri S, Wakode S. PMC3116318
21716619 Abstarct:"BACKGROUND:Moringa peregrina is a wild plant that grown in the eastern desert mountains in Egypt. Although, this plant is native to Egypt, no details studies were traced on its chemical composition and biological activity.MATERIALS AND METHODS:The different fractions of the ethanolic extract of the dried aerial parts of the plants were subjected to fractionation and purification on various silica and sephadex columns for the isolation of the major compounds which were tested for there anticancer activity. The aqueous and ethanolic extract as well as its different fractions were tested for antihyperglycemic effect on Streptozitocin-induced diabetes in rats.RESULTS:Investigation of the different fractions of the ethanolic extract of the aerial parts of M. peregrina yielded lupeol acetate (1), β-amyrin (2), α-amyrin (3), β-sitosterol (4), β-sitosterol-3-O-glucoside (5), apigenin (6), rhamnetin (7), neochlorogenic acid (10), rhamnetin-3-O-rutinoside (12), and 6-methoxy-acacetin-8-C-β-glucoside (13) which were isolated for the first time from the plant. Compound (13) was isolated for the first time from genus Moringa. In addition, quercetin (8), chryseriol-7-O-rhamnoside (9) and quercetin-3-O-rutinoside (11) were also isolated. Identification has been established by spectral data (UV, MS, IR, 1H, 1H -1H COSY, and 13C-NMR). The major isolated compounds were found to have valuable cytotoxic activities against breast (MCF 7) and colon (HCT 116) cancer cell lines and their activities were comparable to the reference drug doxorubicin. On the other hand, the aqueous and ethanolic extracts as well as the n-hexane fraction were found to have potent antihyperglycemic effect on Streptozitocin-induced diabetes in rats.CONCLUSION:The Egyptian plant M. peregrina is rich in biologically active ingredients which showed potent cytotoxic activity and also its ethanolic extraxt exert a significant antihyperglycemic effect." Isolation of biologically active constituents from Moringa peregrina (Forssk.) Fiori. (family: Moringaceae) growing in Egypt. El-Alfy TS, Ezzat SM, Hegazy AK, Amer AM, Kamel GM. PMC3113348
21717161 OBJECTIVE To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn. (A.E.), a Chinese medicinal herb, against coxsackievirus B3 (CVB3). METHODS The antiviral effects of A.E. against CVB3 in vitro (primarily cultured myocardial cells) and in vivo (BALB/c mice) were determined. Serum pharmacological method was also adopted by in vitro experiments. The effects of A.E. inhibiting the CVB3 mRNA expression were compared by RT-PCR in mice in vivo.RESULTS A.E. exhibited obvious antiviral: effects in vivo, and serum samples obtained from the rats with oral administration of A.E. (10 μg/mL, 5 μg/mL), reduced the virus titers in the infected myocardial cells (3.00±0.70, 3.55±0.52, P<0.01). Meanwhile, the viral myocarditis induced by CVB3 was inhibited significantly by A.E., and the 15-day mortality was reduced to 40% and 45% (P<0.01) in mice treated with A.E. at doses of 50 mg/kg and 100 mg/kg, respectively, while the 30-day mortality was decreased to 45% and 50%, respectively (P<0.01). Moreover, the mRNA expression of Coxsackie virus B3 was significantly inhibited by A.E.CONCLUSION Aqueous extract of Spatholobus suberectus Dunn. (A.E.) has inhibitory effect on CVB3 both in vitro and in vivo. Antiviral effects of aqueous extract from Spatholobus suberectus Dunn. against coxsackievirus B3 in mice. Pang J, Guo JP, Jin M, Chen ZQ, Wang XW, Li JW No
21718504
Antibacterial activity of some selected medicinal plants of Pakistan. Bibi Y, Nisa S, Chaudhary FM, Zia M. PMC3141602
21751496 In order to find the anti-virus constituents of Alternanthera philoxeroides (Mart.) Griseb, the investigation was carried out. The paper reported the five triterpenoid saponins isolated from n-BuOH fraction: 3-O-beta-D-glucopyranosyl (1-->3)-O-[beta-D-glucopyranosyl-oleanolic acid]-28-O-beta-D-glucuronopyranoside (1), oleanolic acid-3-O-beta-D-glucuronopyranoside (calenduloside E, 2), oleanolic acid-3-O-beta-D-glucopyranosyl-28-Obeta-D-glucopyranosyl ester (chikusetsusaponin-IVa, 3), 3-O-(6'-O-butyl-beta-D-glucuronopyranosyl)-oleanolic acid-28-O-beta-D-glucopyranosyl ester (4) and hederagenin-3-O-beta-D-glucuronopyranoside (HN-sapoins K, 5). 1 is a new compound, saponins 4 and 5 were isolated from the plant for the first time. [Triterpenoid saponins of Alternanthera philoxeroides (Mart.) Griseb]. Guo QL, Li B, Li J, Li JJ, Xia LY, Dong JX. No
21751849 A new diterpenoid, 15(S)-isopimar-7-en-1-oxo-15,16-diol (1), was isolated from the stems of mangrove plant Rhizophora apiculata. The structure of the new compound was elucidated by MS, IR, 1D, and 2D NMR techniques, including HMQC, HMBC, and NOESY correlations. In addition, seven known constituents were isolated from this plant for the first time. A new diterpenoid from Rhizophora apiculata. Gao MZ, Yuan XY, Cheng MC, Xiao HB, Bao SX. No
21762538 Abstarct:"BACKGROUND:Hepatitis C virus is a major cause of chronic liver diseases which can lead to permanent liver damage, hepatocellular carcinoma and death. The presently available treatment with interferon plus ribavirin, has limited benefits due to adverse side effects such as anemia, depression, fatigue, and ""flu-like"" symptoms. Herbal plants have been used for centuries against different diseases including viral diseases and have become a major source of new compounds to treat bacterial and viral diseases.MATERIAL:The present study was design to study the antiviral effect of Glycyrrhizin (GL) against HCV. For this purpose, HCV infected liver cells were treated with GL at non toxic doses and HCV titer was measured by Quantitative real time RT-PCR. Glycyrrhizin as antiviral agent against Hepatitis C Virus. Ashfaq UA, Masoud MS, Nawaz Z, Riazuddin S. No
21772760 Bioassay-guided isolation of methanolic extract of the leaves of Origanum vulgare Linn., yielded two protocatechuic acid ester derivatives, origanol A (1) and origanol B (2) along with ursolic acid (3), oleanolic acid (4), β-sitosterol (5), and triacontanol (6). Structures of the compound were established based on physical and spectral data (UV, IR, (1)H and (13)C NMR and mass). Origanol A (1) showed significant mushroom tyrosinase inhibition activity. Chemical constituents and biological studies of Origanum vulgare Linn. Venkateswara Rao G, Mukhopadhyay T, Annamalai T, Radhakrishnan N, Sahoo MR. PMC3129025
21808141 BACKGROUND & OBJECTIVES:

Mosquitoes transmit serious human diseases, causing millions of deaths every year and the development of resistance to chemical insecticides resulting in rebounding vectorial capacity. Plants may be alternative sources of mosquito control agents. The present study assessed the role of larvicidal activities of hexane, chloroform, ethyl acetate, acetone, and methanol dried leaf and bark extracts of Annona squamosa L., Chrysanthemum indicum L., and Tridax procumbens L. against the fourth instar larvae of malaria vector, Anopheles subpictus Grassi and Japanese encephalitis vector, Culex tritaeniorhynchus Giles (Diptera: Culicidae).
METHODS:

Larvicidal activities of three medicinal plant extracts were studied in the range of 4